Restriction map for
pF1KB4976
(Circular) (Six-base) MAPSORT of: pF1KB4976.seq Check: 7176 from: 1 to: 4367
pF1KB4976 4367 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 167 920 1405 167
Size: 753 485 3129
Fragments arranged by size:
3129 753 485
Acc65I G'GTAC_C
Cuts at: 1333 1383 1333
Size: 50 4317
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2192 2624 3371 3639 2192
Size: 432 747 268 2920
Fragments arranged by size:
2920 747 432 268
AflIII A'CryG_T
Cuts at: 1719 2823 3800 1719
Size: 1104 977 2286
Fragments arranged by size:
2286 1104 977
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 846 846
Size: 4367
AlwNI CAG_nnn'CTG
Cuts at: 1293 1672 3239 1293
Size: 379 1567 2421
Fragments arranged by size:
2421 1567 379
ApaI G_GGCC'C
Cuts at: 506 506
Size: 4367
ApaLI G'TGCA_C
Cuts at: 3137 3137
Size: 4367
ApoI r'AATT_y
Cuts at: 564 591 1371 3720 3976 564
Size: 27 780 2349 256 955
Fragments arranged by size:
2349 955 780 256 27
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 125 379 1097 1387 125
Size: 254 718 290 3105
Fragments arranged by size:
3105 718 290 254
BamHI G'GATC_C
Cuts at: 237 1392 237
Size: 1155 3212
BanI G'GyrC_C
Cuts at: 894 1033 1333 1383 1797 2045 2080 894
Size: 139 300 50 414 248 35 3181
Fragments arranged by size:
3181 414 300 248 139 50 35
BanII G_rGCy'C
Cuts at: 506 1381 2411 506
Size: 875 1030 2462
Fragments arranged by size:
2462 1030 875
BbeI G_GCGC'C
Cuts at: 1801 2049 1801
Size: 248 4119
BbsI GAAGACnn'nnnn_
Cuts at: 515 1001 515
Size: 486 3881
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 884 918 884
Size: 34 4333
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 1887 1887
Size: 4367
BglII A'GATC_T
Cuts at: 725 803 1116 1882 3919 725
Size: 78 313 766 2037 1173
Fragments arranged by size:
2037 1173 766 313 78
BlpI GC'TnA_GC
Cuts at: 1479 1479
Size: 4367
Bme1580I G_kGCm'C
Cuts at: 506 862 1992 2085 3141 506
Size: 356 1130 93 1056 1732
Fragments arranged by size:
1732 1130 1056 356 93
BmgBI CAC'GTC
Cuts at: 765 1134 765
Size: 369 3998
BmrI ACTGGGnnnn_n'
Cuts at: 1579 1660 1995 4139 1579
Size: 81 335 2144 1807
Fragments arranged by size:
2144 1807 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 1543 2422 2914 3212 970
Size: 573 879 492 298 2125
Fragments arranged by size:
2125 879 573 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 655 4071 655
Size: 3416 951
BsaAI yAC'GTr
Cuts at: 1720 1720
Size: 4367
BsaBI GATnn'nnATC
Cuts at: 6 802 1229 1881 3918 6
Size: 796 427 652 2037 455
Fragments arranged by size:
2037 796 652 455 427
BsaHI Gr'CG_yC
Cuts at: 1216 1798 2046 2748 4244 1216
Size: 582 248 702 1496 1339
Fragments arranged by size:
1496 1339 702 582 248
BsaWI w'CCGG_w
Cuts at: 611 1556 2077 2755 3029 3176 3587 611
Size: 945 521 678 274 147 411 1391
Fragments arranged by size:
1391 945 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1326 1356 1326
Size: 30 4337
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 586 1339 586
Size: 753 3614
BseYI C'CCAG_C
Cuts at: 429 647 898 1792 2550 3127 3623 429
Size: 218 251 894 758 577 496 1173
Fragments arranged by size:
1173 894 758 577 496 251 218
BsiEI CG_ry'CG
Cuts at: 104 1909 1955 3163 104
Size: 1805 46 1208 1308
Fragments arranged by size:
1805 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 440 1381 2159 2349 3141 440
Size: 941 778 190 792 1666
Fragments arranged by size:
1666 941 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1207 1207
Size: 4367
Bsp1286I G_dGCh'C
Cuts at: 440 506 862 1381 1992 2085 2159 2349 2411
Size: 66 356 519 611 93 74 190 62
Cuts at: 2411 3141 440
Size: 730 1666
Fragments arranged by size:
1666 730 611 519 356 190 93 74
66 62
BspEI T'CCGG_A
Cuts at: 611 3587 611
Size: 2976 1391
BspHI T'CATG_A
Cuts at: 3543 3543
Size: 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 289 1417 1631 1933 2314 289
Size: 1128 214 302 381 2342
Fragments arranged by size:
2342 1128 381 302 214
BsrBI CCG'CTC
Cuts at: 50 258 2659 2713 4197 4362 50
Size: 208 2401 54 1484 165 55
Fragments arranged by size:
2401 1484 208 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2279 2279
Size: 4367
BsrFI r'CCGG_y
Cuts at: 372 1336 2365 2546 2755 372
Size: 964 1029 181 209 1984
Fragments arranged by size:
1984 1029 964 209 181
BssSI C'ACGA_G
Cuts at: 205 2638 2996 205
Size: 2433 358 1576
Fragments arranged by size:
2433 1576 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1744 1744
Size: 4367
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstXI CCAn_nnnn'nTGG
Cuts at: 1134 1134
Size: 4367
BstYI r'GATC_y
Cuts at: 1 237 461 578 725 803 1046 1116 1392
Size: 236 224 117 147 78 243 70 276
Cuts at: 1392 1859 1882 2217 2463 3475 3561 3573 3919
Size: 467 23 335 246 1012 86 12 346
Cuts at: 3919 1
Size: 449
Fragments arranged by size:
1012 467 449 346 335 276 246 243
236 224 147 117 86 78 70 23
12
BtgI C'CryG_G
Cuts at: 107 735 869 1139 107
Size: 628 134 270 3335
Fragments arranged by size:
3335 628 270 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2317 2498 2661 2317
Size: 181 163 4023
Fragments arranged by size:
4023 181 163
BtsI GCAGTG_nn'
Cuts at: 490 1610 490
Size: 1120 3247
DraI TTT'AAA
Cuts at: 1366 1366
Size: 4367
DrdI GACnn_nn'nnGTC
Cuts at: 313 541 2073 2931 313
Size: 228 1532 858 1749
Fragments arranged by size:
1749 1532 858 228
EaeI y'GGCC_r
Cuts at: 297 350 1136 1952 2126 2517 2544 3889 297
Size: 53 786 816 174 391 27 1345 775
Fragments arranged by size:
1345 816 786 775 391 174 53 27
EagI C'GGCC_G
Cuts at: 1952 1952
Size: 4367
EarI CTCTTCn'nnn_
Cuts at: 869 1316 2390 2600 869
Size: 447 1074 210 2636
Fragments arranged by size:
2636 1074 447 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2575 2885 3031 4177 2575
Size: 310 146 1146 2765
Fragments arranged by size:
2765 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1379 1379
Size: 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2192 2624 3371 3639 2192
Size: 432 747 268 2920
Fragments arranged by size:
2920 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 122 306 339 502 963 1506 122
Size: 184 33 163 461 543 2983
Fragments arranged by size:
2983 543 461 184 163 33
EcoRI G'AATT_C
Cuts at: 591 1371 591
Size: 780 3587
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 201 2386 201
Size: 2185 2182
FspI TGC'GCA
Cuts at: 2148 2148
Size: 4367
HaeII r_GCGC'y
Cuts at: 1801 2049 3071 4050 1801
Size: 248 1022 979 2118
Fragments arranged by size:
2118 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 731 763 1108 1140 2209 2239 2241 2271 731
Size: 32 345 32 1069 30 2 30 2827
Fragments arranged by size:
2827 1069 345 32 32 30 30 2
HincII GTy'rAC
Cuts at: 756 1406 756
Size: 650 3717
KasI G'GCGC_C
Cuts at: 1797 2045 1797
Size: 248 4119
KpnI G_GTAC'C
Cuts at: 1337 1387 1337
Size: 50 4317
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 665 3038 3222 665
Size: 2373 184 1810
Fragments arranged by size:
2373 1810 184
MscI TGG'CCA
Cuts at: 352 2128 352
Size: 1776 2591
MslI CAynn'nnrTG
Cuts at: 1159 2356 2483 1159
Size: 1197 127 3043
Fragments arranged by size:
3043 1197 127
MspA1I CmG'CkG
Cuts at: 1478 1792 2152 3165 3410 1478
Size: 314 360 1013 245 2435
Fragments arranged by size:
2435 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2548 2548
Size: 4367
NarI GG'CG_CC
Cuts at: 1798 2046 1798
Size: 248 4119
NcoI C'CATG_G
Cuts at: 107 735 869 107
Size: 628 134 3605
Fragments arranged by size:
3605 628 134
NgoMIV G'CCGG_C
Cuts at: 2546 2546
Size: 4367
NspI r_CATG'y
Cuts at: 1420 1920 2827 1420
Size: 500 907 2960
Fragments arranged by size:
2960 907 500
PciI A'CATG_T
Cuts at: 2823 2823
Size: 4367
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 994 1026 3506 3538 994
Size: 32 2480 32 1823
Fragments arranged by size:
2480 1823 32 32
PpuMI rG'GwC_Cy
Cuts at: 122 306 339 122
Size: 184 33 4150
Fragments arranged by size:
4150 184 33
PspOMI G'GGCC_C
Cuts at: 502 502
Size: 4367
PstI C_TGCA'G
Cuts at: 410 497 1414 2099 410
Size: 87 917 685 2678
Fragments arranged by size:
2678 917 685 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 1792 2152 1792
Size: 360 4007
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 1381 1381
Size: 4367
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SanDI GG'GwC_CC
Cuts at: 122 122
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 2390 2600 2390
Size: 210 4157
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SfcI C'TryA_G
Cuts at: 33 406 493 1410 2095 3088 3279 33
Size: 373 87 917 685 993 191 1121
Fragments arranged by size:
1121 993 917 685 373 191 87
SfoI GGC'GCC
Cuts at: 1799 2047 1799
Size: 248 4119
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 1389 1389
Size: 4367
SmlI C'TyrA_G
Cuts at: 985 1522 2437 2929 3191 985
Size: 537 915 492 262 2161
Fragments arranged by size:
2161 915 537 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 1420 1920 1420
Size: 500 3867
StuI AGG'CCT
Cuts at: 639 639
Size: 4367
StyI C'CwwG_G
Cuts at: 107 735 869 971 1501 1855 107
Size: 628 134 102 530 354 2619
Fragments arranged by size:
2619 628 530 354 134 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 912 1950 2083 2542 2751 912
Size: 1038 133 459 209 2528
Fragments arranged by size:
2528 1038 459 209 133
TatI w'GTAC_w
Cuts at: 851 3882 851
Size: 3031 1336
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3884 3916 3884
Size: 32 4335
Tth111I GACn'n_nGTC
Cuts at: 2164 2164
Size: 4367
XbaI T'CTAG_A
Cuts at: 1398 1398
Size: 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1862 1862
Size: 4367
XmaI C'CCGG_G
Cuts at: 1387 1387
Size: 4367
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI BpmI Bpu10I
BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI