Restriction map for
pF1KB4961
(Circular) (Six-base) MAPSORT of: pF1KB4961.seq Check: 5980 from: 1 to: 3767
pF1KB4961 3767 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 805 805
Size: 3767
Acc65I G'GTAC_C
Cuts at: 783 783
Size: 3767
AclI AA'CG_TT
Cuts at: 3607 3607
Size: 3767
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 301 1592 2024 2771 3039 275
Size: 26 1291 432 747 268 1003
Fragments arranged by size:
1291 1003 747 432 268 26
AflIII A'CryG_T
Cuts at: 255 1119 2223 3200 255
Size: 864 1104 977 822
Fragments arranged by size:
1104 977 864 822
AgeI A'CCGG_T
Cuts at: 2155 2155
Size: 3767
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 482 482
Size: 3767
AlwNI CAG_nnn'CTG
Cuts at: 443 604 1072 2639 443
Size: 161 468 1567 1571
Fragments arranged by size:
1571 1567 468 161
ApaLI G'TGCA_C
Cuts at: 2537 2537
Size: 3767
ApoI r'AATT_y
Cuts at: 205 217 711 771 3120 3376 205
Size: 12 494 60 2349 256 596
Fragments arranged by size:
2349 596 494 256 60 12
AseI AT'TA_AT
Cuts at: 20 397 496 20
Size: 377 99 3291
Fragments arranged by size:
3291 377 99
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
AvaI C'yCGr_G
Cuts at: 151 551 787 151
Size: 400 236 3131
Fragments arranged by size:
3131 400 236
BamHI G'GATC_C
Cuts at: 792 792
Size: 3767
BanI G'GyrC_C
Cuts at: 783 1197 1445 1480 783
Size: 414 248 35 3070
Fragments arranged by size:
3070 414 248 35
BanII G_rGCy'C
Cuts at: 781 1811 781
Size: 1030 2737
BbeI G_GCGC'C
Cuts at: 1201 1449 1201
Size: 248 3519
BbsI GAAGACnn'nnnn_
Cuts at: 178 685 178
Size: 507 3260
BciVI GTATCCnnnnn_n'
Cuts at: 1665 2426 3767 1665
Size: 761 1341 1665
Fragments arranged by size:
1665 1341 761
BclI T'GATC_A
Cuts at: 134 1287 134
Size: 1153 2614
BglII A'GATC_T
Cuts at: 1282 3319 1282
Size: 2037 1730
BlpI GC'TnA_GC
Cuts at: 879 879
Size: 3767
Bme1580I G_kGCm'C
Cuts at: 1392 1485 2541 1392
Size: 93 1056 2618
Fragments arranged by size:
2618 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 979 1060 1395 3539 979
Size: 81 335 2144 1207
Fragments arranged by size:
2144 1207 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 3767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1822 2314 2612 943
Size: 879 492 298 2098
Fragments arranged by size:
2098 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3471 3471
Size: 3767
BsaAI yAC'GTr
Cuts at: 1120 1120
Size: 3767
BsaBI GATnn'nnATC
Cuts at: 6 1281 3318 6
Size: 1275 2037 455
Fragments arranged by size:
2037 1275 455
BsaHI Gr'CG_yC
Cuts at: 380 1198 1446 2148 3644 380
Size: 818 248 702 1496 503
Fragments arranged by size:
1496 818 702 503 248
BsaWI w'CCGG_w
Cuts at: 350 956 1477 2155 2429 2576 2987 350
Size: 606 521 678 274 147 411 1130
Fragments arranged by size:
1130 678 606 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1192 1950 2527 3023 1192
Size: 758 577 496 1936
Fragments arranged by size:
1936 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 617 617
Size: 3767
BsiEI CG_ry'CG
Cuts at: 104 1309 1355 2563 104
Size: 1205 46 1208 1308
Fragments arranged by size:
1308 1208 1205 46
BsiHKAI G_wGCw'C
Cuts at: 781 1559 1749 2541 781
Size: 778 190 792 2007
Fragments arranged by size:
2007 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 371 371
Size: 3767
Bsp1286I G_dGCh'C
Cuts at: 781 1392 1485 1559 1749 1811 2541 781
Size: 611 93 74 190 62 730 2007
Fragments arranged by size:
2007 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 350 2987 350
Size: 2637 1130
BspHI T'CATG_A
Cuts at: 650 2943 650
Size: 2293 1474
BspMI ACCTGCnnnn'nnnn_
Cuts at: 817 1031 1333 1714 817
Size: 214 302 381 2870
Fragments arranged by size:
2870 381 302 214
BsrBI CCG'CTC
Cuts at: 50 345 2059 2113 3597 3762 50
Size: 295 1714 54 1484 165 55
Fragments arranged by size:
1714 1484 295 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1679 1679
Size: 3767
BsrFI r'CCGG_y
Cuts at: 1765 1946 2155 1765
Size: 181 209 3377
Fragments arranged by size:
3377 209 181
BssSI C'ACGA_G
Cuts at: 2038 2396 2038
Size: 358 3409
BstAPI GCAn_nnn'nTGC
Cuts at: 1144 1144
Size: 3767
BstBI TT'CG_AA
Cuts at: 2128 2128
Size: 3767
BstYI r'GATC_y
Cuts at: 1 286 792 1259 1282 1617 1863 2875 2961
Size: 285 506 467 23 335 246 1012 86
Cuts at: 2961 2973 3319 1
Size: 12 346 449
Fragments arranged by size:
1012 506 467 449 346 335 285 246
86 23 12
BtgI C'CryG_G
Cuts at: 383 383
Size: 3767
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1717 1898 2061 1717
Size: 181 163 3423
Fragments arranged by size:
3423 181 163
BtsI GCAGTG_nn'
Cuts at: 329 362 1010 329
Size: 33 648 3086
Fragments arranged by size:
3086 648 33
DraI TTT'AAA
Cuts at: 306 766 306
Size: 460 3307
DrdI GACnn_nn'nnGTC
Cuts at: 1473 2331 1473
Size: 858 2909
EaeI y'GGCC_r
Cuts at: 1352 1526 1917 1944 3289 1352
Size: 174 391 27 1345 1830
Fragments arranged by size:
1830 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1352 1352
Size: 3767
EarI CTCTTCn'nnn_
Cuts at: 1790 2000 1790
Size: 210 3557
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1975 2285 2431 3577 1975
Size: 310 146 1146 2165
Fragments arranged by size:
2165 1146 310 146
EcoICRI GAG'CTC
Cuts at: 779 779
Size: 3767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 301 1592 2024 2771 3039 275
Size: 26 1291 432 747 268 1003
Fragments arranged by size:
1291 1003 747 432 268 26
EcoO109I rG'GnC_Cy
Cuts at: 906 906
Size: 3767
EcoRI G'AATT_C
Cuts at: 771 771
Size: 3767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 331 1786 331
Size: 1455 2312
FspI TGC'GCA
Cuts at: 1548 1548
Size: 3767
HaeII r_GCGC'y
Cuts at: 1201 1449 2471 3450 1201
Size: 248 1022 979 1518
Fragments arranged by size:
1518 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1609 1639 1641 1671 1609
Size: 30 2 30 3705
Fragments arranged by size:
3705 30 30 2
HincII GTy'rAC
Cuts at: 418 806 418
Size: 388 3379
KasI G'GCGC_C
Cuts at: 1197 1445 1197
Size: 248 3519
KpnI G_GTAC'C
Cuts at: 787 787
Size: 3767
MluI A'CGCG_T
Cuts at: 3200 3200
Size: 3767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2438 2622 2438
Size: 184 3583
MscI TGG'CCA
Cuts at: 1528 1528
Size: 3767
MslI CAynn'nnrTG
Cuts at: 1756 1883 1756
Size: 127 3640
MspA1I CmG'CkG
Cuts at: 878 1192 1552 2565 2810 878
Size: 314 360 1013 245 1835
Fragments arranged by size:
1835 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1948 1948
Size: 3767
NarI GG'CG_CC
Cuts at: 1198 1446 1198
Size: 248 3519
NcoI C'CATG_G
Cuts at: 383 383
Size: 3767
NgoMIV G'CCGG_C
Cuts at: 1946 1946
Size: 3767
NspI r_CATG'y
Cuts at: 820 1320 2227 820
Size: 500 907 2360
Fragments arranged by size:
2360 907 500
PciI A'CATG_T
Cuts at: 2223 2223
Size: 3767
PmeI GTTT'AAAC
Cuts at: 766 766
Size: 3767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2906 2938 2906
Size: 32 3735
PsiI TTA'TAA
Cuts at: 266 266
Size: 3767
PstI C_TGCA'G
Cuts at: 814 1499 814
Size: 685 3082
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3767
PvuII CAG'CTG
Cuts at: 1192 1552 1192
Size: 360 3407
RsrII CG'GwC_CG
Cuts at: 1962 1962
Size: 3767
SacI G_AGCT'C
Cuts at: 781 781
Size: 3767
SalI G'TCGA_C
Cuts at: 804 804
Size: 3767
SapI GCTCTTCn'nnn_
Cuts at: 1790 2000 1790
Size: 210 3557
SbfI CC_TGCA'GG
Cuts at: 814 814
Size: 3767
ScaI AGT'ACT
Cuts at: 3284 3284
Size: 3767
SfcI C'TryA_G
Cuts at: 33 227 626 810 1495 2488 2679 33
Size: 194 399 184 685 993 191 1121
Fragments arranged by size:
1121 993 685 399 194 191 184
SfoI GGC'GCC
Cuts at: 1199 1447 1199
Size: 248 3519
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
SmaI CCC'GGG
Cuts at: 789 789
Size: 3767
SmlI C'TyrA_G
Cuts at: 922 1837 2329 2591 922
Size: 915 492 262 2098
Fragments arranged by size:
2098 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3767
SphI G_CATG'C
Cuts at: 820 1320 820
Size: 500 3267
StyI C'CwwG_G
Cuts at: 383 901 1255 383
Size: 518 354 2895
Fragments arranged by size:
2895 518 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 294 1350 1483 1942 2151 127
Size: 167 1056 133 459 209 1743
Fragments arranged by size:
1743 1056 459 209 167 133
TatI w'GTAC_w
Cuts at: 3282 3282
Size: 3767
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 599 631 3284 3316 599
Size: 32 2653 32 1050
Fragments arranged by size:
2653 1050 32 32
Tth111I GACn'n_nGTC
Cuts at: 1564 1564
Size: 3767
XbaI T'CTAG_A
Cuts at: 798 798
Size: 3767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1262 1262
Size: 3767
XmaI C'CCGG_G
Cuts at: 787 787
Size: 3767
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI