Restriction map for
pF1KB4953
(Circular) (Six-base) MAPSORT of: pF1KB4953.seq Check: 9242 from: 1 to: 6560
pF1KB4953 6560 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AatII G_ACGT'C
Cuts at: 1819 1819
Size: 6560
AccI GT'mk_AC
Cuts at: 911 3598 911
Size: 2687 3873
Acc65I G'GTAC_C
Cuts at: 3576 3576
Size: 6560
AclI AA'CG_TT
Cuts at: 6400 6400
Size: 6560
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1999 2429 2935 3527 4385 4817 5170 5832 1999
Size: 430 506 592 858 432 353 662 2727
Fragments arranged by size:
2727 858 662 592 506 432 430 353
AflII C'TTAA_G
Cuts at: 961 961
Size: 6560
AflIII A'CryG_T
Cuts at: 2819 3912 5712 5993 2819
Size: 1093 1800 281 3386
Fragments arranged by size:
3386 1800 1093 281
AleI CACnn'nnGTG
Cuts at: 1531 2504 3022 1531
Size: 973 518 5069
Fragments arranged by size:
5069 973 518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1378 2150 1378
Size: 772 5788
AlwNI CAG_nnn'CTG
Cuts at: 1267 3321 3865 5303 1267
Size: 2054 544 1438 2524
Fragments arranged by size:
2524 2054 1438 544
ApaLI G'TGCA_C
Cuts at: 1377 5398 1377
Size: 4021 2539
ApoI r'AATT_y
Cuts at: 250 942 998 1134 1207 1498 1617 2586 3564
Size: 692 56 136 73 291 119 969 978
Cuts at: 3564 5913 6169 250
Size: 2349 256 641
Fragments arranged by size:
2349 978 969 692 641 291 256 136
119 73 56
AseI AT'TA_AT
Cuts at: 20 20
Size: 6560
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6560
AvaI C'yCGr_G
Cuts at: 3580 3580
Size: 6560
BamHI G'GATC_C
Cuts at: 2108 3585 2108
Size: 1477 5083
BanI G'GyrC_C
Cuts at: 829 2605 3576 3990 4238 4273 829
Size: 1776 971 414 248 35 3116
Fragments arranged by size:
3116 1776 971 414 248 35
BanII G_rGCy'C
Cuts at: 638 873 1054 3574 4604 638
Size: 235 181 2520 1030 2594
Fragments arranged by size:
2594 2520 1030 235 181
BbeI G_GCGC'C
Cuts at: 3994 4242 3994
Size: 248 6312
BbsI GAAGACnn'nnnn_
Cuts at: 172 193 172
Size: 21 6539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1754 1788 90
Size: 34 1630 34 4862
Fragments arranged by size:
4862 1630 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4458 5514 6560 4458
Size: 1056 1046 4458
Fragments arranged by size:
4458 1056 1046
BclI T'GATC_A
Cuts at: 4080 4080
Size: 6560
BglII A'GATC_T
Cuts at: 2681 4075 6112 2681
Size: 1394 2037 3129
Fragments arranged by size:
3129 2037 1394
BlpI GC'TnA_GC
Cuts at: 1053 3672 1053
Size: 2619 3941
Bme1580I G_kGCm'C
Cuts at: 1381 4185 4278 5402 1381
Size: 2804 93 1124 2539
Fragments arranged by size:
2804 2539 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 558 850 1675 3010 3772 3853 4188 6332 558
Size: 292 825 1335 762 81 335 2144 786
Fragments arranged by size:
2144 1335 825 786 762 335 292 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 159 207 4927 159
Size: 48 4720 1792
Fragments arranged by size:
4720 1792 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1562 3227 3343 1562
Size: 1665 116 4779
Fragments arranged by size:
4779 1665 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3320 3376 3461 3736 4615 5329 5627 3320
Size: 56 85 275 879 714 298 4253
Fragments arranged by size:
4253 879 714 298 275 85 56
BsaI GGTCTCn'nnnn_
Cuts at: 2749 6264 2749
Size: 3515 3045
BsaAI yAC'GTr
Cuts at: 3417 3913 3417
Size: 496 6064
BsaBI GATnn'nnATC
Cuts at: 6 4074 6111 6
Size: 4068 2037 455
Fragments arranged by size:
4068 2037 455
BsaHI Gr'CG_yC
Cuts at: 1816 3991 4239 4941 6437 1816
Size: 2175 248 702 1496 1939
Fragments arranged by size:
2175 1939 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2264 3749 4270 4948 5359 5506 5780 2264
Size: 1485 521 678 411 147 274 3044
Fragments arranged by size:
3044 1485 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 560 590 1580 1610 2611 2641 3338 3368 560
Size: 30 990 30 1001 30 697 30 3752
Fragments arranged by size:
3752 1001 990 697 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 881 2384 881
Size: 1503 5057
BseYI C'CCAG_C
Cuts at: 239 3985 4743 5408 5816 239
Size: 3746 758 665 408 983
Fragments arranged by size:
3746 983 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1466 1981 1466
Size: 515 6045
BsiEI CG_ry'CG
Cuts at: 104 4102 4148 5378 104
Size: 3998 46 1230 1286
Fragments arranged by size:
3998 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 873 1381 2669 3574 4352 4542 5402 873
Size: 508 1288 905 778 190 860 2031
Fragments arranged by size:
2031 1288 905 860 778 508 190
BsmI GAATG_Cn'
Cuts at: 1074 2862 1074
Size: 1788 4772
BsmBI CGTCTCn'nnnn_
Cuts at: 1807 1807
Size: 6560
Bsp1286I G_dGCh'C
Cuts at: 638 873 1054 1381 2669 3574 4185 4278 4352
Size: 235 181 327 1288 905 611 93 74
Cuts at: 4352 4542 4604 5402 638
Size: 190 62 798 1796
Fragments arranged by size:
1796 1288 905 798 611 327 235 190
181 93 74 62
BspEI T'CCGG_A
Cuts at: 2264 2264
Size: 6560
BspHI T'CATG_A
Cuts at: 4992 4992
Size: 6560
BspMI ACCTGCnnnn'nnnn_
Cuts at: 612 3610 3824 4126 4507 612
Size: 2998 214 302 381 2665
Fragments arranged by size:
2998 2665 381 302 214
BsrBI CCG'CTC
Cuts at: 50 143 3431 4852 4906 6390 6555 50
Size: 93 3288 1421 54 1484 165 55
Fragments arranged by size:
3288 1484 1421 165 93 55 54
BsrDI GCAATG_nn'
Cuts at: 1362 1447 2529 2774 4472 1362
Size: 85 1082 245 1698 3450
Fragments arranged by size:
3450 1698 1082 245 85
BsrFI r'CCGG_y
Cuts at: 4558 4739 4558
Size: 181 6379
BsrGI T'GTAC_A
Cuts at: 993 3014 3266 993
Size: 2021 252 4287
Fragments arranged by size:
4287 2021 252
BssHII G'CGCG_C
Cuts at: 147 147
Size: 6560
BssSI C'ACGA_G
Cuts at: 4831 5539 4831
Size: 708 5852
BstAPI GCAn_nnn'nTGC
Cuts at: 3937 3937
Size: 6560
BstBI TT'CG_AA
Cuts at: 4921 4921
Size: 6560
BstXI CCAn_nnnn'nTGG
Cuts at: 3162 3162
Size: 6560
BstYI r'GATC_y
Cuts at: 1 137 1236 2108 2681 2717 3585 4052 4075
Size: 136 1099 872 573 36 868 467 23
Cuts at: 4075 4410 4656 4962 4974 5060 6112 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1099 1052 872 868 573 467 449 335
306 246 136 86 36 23 12
BtgI C'CryG_G
Cuts at: 3459 3459
Size: 6560
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3090 4510 4691 4854 3090
Size: 1420 181 163 4796
Fragments arranged by size:
4796 1420 181 163
BtsI GCAGTG_nn'
Cuts at: 272 361 500 1265 1538 3803 272
Size: 89 139 765 273 2265 3029
Fragments arranged by size:
3029 2265 765 273 139 89
DraI TTT'AAA
Cuts at: 2900 3294 3559 2900
Size: 394 265 5901
Fragments arranged by size:
5901 394 265
DrdI GACnn_nn'nnGTC
Cuts at: 4266 5610 4266
Size: 1344 5216
EaeI y'GGCC_r
Cuts at: 1308 4145 4319 4710 4737 6082 1308
Size: 2837 174 391 27 1345 1786
Fragments arranged by size:
2837 1786 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4145 4145
Size: 6560
EarI CTCTTCn'nnn_
Cuts at: 324 1979 2402 2914 4583 4793 324
Size: 1655 423 512 1669 210 2091
Fragments arranged by size:
2091 1669 1655 512 423 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4768 5510 5656 6370 4768
Size: 742 146 714 4958
Fragments arranged by size:
4958 742 714 146
EcoICRI GAG'CTC
Cuts at: 871 3572 871
Size: 2701 3859
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1562 1999 2429 2935 3227 3343 3527 4385 4817
Size: 437 430 506 292 116 184 858 432
Cuts at: 4817 5170 5832 1562
Size: 353 662 2290
Fragments arranged by size:
2290 858 662 506 437 432 430 353
292 184 116
EcoO109I rG'GnC_Cy
Cuts at: 3699 3699
Size: 6560
EcoRI G'AATT_C
Cuts at: 942 3564 942
Size: 2622 3938
EcoRV GAT'ATC
Cuts at: 723 723
Size: 6560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2224 2655 3417 4579 2224
Size: 431 762 1162 4205
Fragments arranged by size:
4205 1162 762 431
FspI TGC'GCA
Cuts at: 4341 4341
Size: 6560
HaeII r_GCGC'y
Cuts at: 1118 3994 4242 5472 6243 1118
Size: 2876 248 1230 771 1435
Fragments arranged by size:
2876 1435 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1548 1580 1612 2705 2737 3316 3348 4402 4432
Size: 32 32 1093 32 579 32 1054 30
Cuts at: 4432 4434 4464 1548
Size: 2 30 3644
Fragments arranged by size:
3644 1093 1054 579 32 32 32 32
30 30 2
HincII GTy'rAC
Cuts at: 735 3599 735
Size: 2864 3696
KasI G'GCGC_C
Cuts at: 3990 4238 3990
Size: 248 6312
KpnI G_GTAC'C
Cuts at: 3580 3580
Size: 6560
MfeI C'AATT_G
Cuts at: 1612 1612
Size: 6560
MluI A'CGCG_T
Cuts at: 5993 5993
Size: 6560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 113 546 689 783 1501 5319 5503 113
Size: 433 143 94 718 3818 184 1170
Fragments arranged by size:
3818 1170 718 433 184 143 94
MscI TGG'CCA
Cuts at: 1310 4321 1310
Size: 3011 3549
MslI CAynn'nnrTG
Cuts at: 1531 2182 2504 3022 3144 3160 3238 3464 4549
Size: 651 322 518 122 16 78 226 1085
Cuts at: 4549 4676 1531
Size: 127 3415
Fragments arranged by size:
3415 1085 651 518 322 226 127 122
78 16
MspA1I CmG'CkG
Cuts at: 243 3671 3985 4345 5129 5374 243
Size: 3428 314 360 784 245 1429
Fragments arranged by size:
3428 1429 784 360 314 245
NaeI GCC'GGC
Cuts at: 4741 4741
Size: 6560
NarI GG'CG_CC
Cuts at: 3991 4239 3991
Size: 248 6312
NcoI C'CATG_G
Cuts at: 3459 3459
Size: 6560
NdeI CA'TA_TG
Cuts at: 3522 3522
Size: 6560
NgoMIV G'CCGG_C
Cuts at: 4739 4739
Size: 6560
NspI r_CATG'y
Cuts at: 1248 1355 2823 3613 4113 5716 1248
Size: 107 1468 790 500 1603 2092
Fragments arranged by size:
2092 1603 1468 790 500 107
PciI A'CATG_T
Cuts at: 2819 5712 2819
Size: 2893 3667
PfoI T'CCnGG_A
Cuts at: 2345 2345
Size: 6560
PmeI GTTT'AAAC
Cuts at: 3559 3559
Size: 6560
PmlI CAC'GTG
Cuts at: 3417 3417
Size: 6560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5006 5038 5006
Size: 32 6528
PsiI TTA'TAA
Cuts at: 580 2194 2290 580
Size: 1614 96 4850
Fragments arranged by size:
4850 1614 96
PstI C_TGCA'G
Cuts at: 371 2537 3607 4292 371
Size: 2166 1070 685 2639
Fragments arranged by size:
2639 2166 1070 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6560
PvuII CAG'CTG
Cuts at: 243 3985 4345 243
Size: 3742 360 2458
Fragments arranged by size:
3742 2458 360
RsrII CG'GwC_CG
Cuts at: 4755 4755
Size: 6560
SacI G_AGCT'C
Cuts at: 873 3574 873
Size: 2701 3859
SalI G'TCGA_C
Cuts at: 3597 3597
Size: 6560
SapI GCTCTTCn'nnn_
Cuts at: 324 4583 4793 324
Size: 4259 210 2091
Fragments arranged by size:
4259 2091 210
SbfI CC_TGCA'GG
Cuts at: 2537 3607 2537
Size: 1070 5490
ScaI AGT'ACT
Cuts at: 6077 6077
Size: 6560
SfcI C'TryA_G
Cuts at: 33 367 1862 2533 3603 4288 5256 5447 33
Size: 334 1495 671 1070 685 968 191 1146
Fragments arranged by size:
1495 1146 1070 968 685 671 334 191
SfoI GGC'GCC
Cuts at: 3992 4240 3992
Size: 248 6312
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6560
SmaI CCC'GGG
Cuts at: 3582 3582
Size: 6560
SmlI C'TyrA_G
Cuts at: 961 3335 3391 3476 3715 4630 5344 5606 961
Size: 2374 56 85 239 915 714 262 1915
Fragments arranged by size:
2374 1915 915 714 262 239 85 56
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6560
SphI G_CATG'C
Cuts at: 3613 4113 3613
Size: 500 6060
SspI AAT'ATT
Cuts at: 2578 2792 2578
Size: 214 6346
StuI AGG'CCT
Cuts at: 2531 2531
Size: 6560
StyI C'CwwG_G
Cuts at: 2250 2876 3459 3694 4048 2250
Size: 626 583 235 354 4762
Fragments arranged by size:
4762 626 583 354 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3105 3388 4143 4276 4735 4944 3105
Size: 283 755 133 459 209 4721
Fragments arranged by size:
4721 755 459 283 209 133
TatI w'GTAC_w
Cuts at: 615 993 2242 3014 3266 3354 6075 615
Size: 378 1249 772 252 88 2721 1100
Fragments arranged by size:
2721 1249 1100 772 378 252 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 285 317 2996 3028 6077 6109 285
Size: 32 2679 32 3049 32 736
Fragments arranged by size:
3049 2679 736 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 129 4357 129
Size: 4228 2332
XbaI T'CTAG_A
Cuts at: 3591 3591
Size: 6560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4055 4055
Size: 6560
XmaI C'CCGG_G
Cuts at: 3580 3580
Size: 6560
XmnI GAAnn'nnTTC
Cuts at: 1074 3495 1074
Size: 2421 4139
ZraI GAC'GTC
Cuts at: 1817 1817
Size: 6560
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PfoI PmeI PmlI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AgeI AhdI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsiWI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI NheI NotI NruI NsiI PacI
PasI PflMI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI