Restriction map for
pF1KB4948
(Circular) (Six-base) MAPSORT of: pF1KB4948.seq Check: 3852 from: 1 to: 4853
pF1KB4948 4853 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 219 1071 219
Size: 852 4001
AatII G_ACGT'C
Cuts at: 129 129
Size: 4853
AccI GT'mk_AC
Cuts at: 701 1891 701
Size: 1190 3663
Acc65I G'GTAC_C
Cuts at: 1869 1869
Size: 4853
AclI AA'CG_TT
Cuts at: 4693 4693
Size: 4853
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2678 3110 3463 4125 2678
Size: 432 353 662 3406
Fragments arranged by size:
3406 662 432 353
AflIII A'CryG_T
Cuts at: 2205 4005 4286 2205
Size: 1800 281 2772
Fragments arranged by size:
2772 1800 281
AleI CACnn'nnGTG
Cuts at: 922 922
Size: 4853
AlwNI CAG_nnn'CTG
Cuts at: 249 1066 1512 1731 2158 3596 249
Size: 817 446 219 427 1438 1506
Fragments arranged by size:
1506 1438 817 446 427 219
ApaLI G'TGCA_C
Cuts at: 3691 3691
Size: 4853
ApoI r'AATT_y
Cuts at: 111 481 1857 4206 4462 111
Size: 370 1376 2349 256 502
Fragments arranged by size:
2349 1376 502 370 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4853
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4853
AvaI C'yCGr_G
Cuts at: 1571 1873 1571
Size: 302 4551
BamHI G'GATC_C
Cuts at: 274 383 1003 1878 274
Size: 109 620 875 3249
Fragments arranged by size:
3249 875 620 109
BanI G'GyrC_C
Cuts at: 236 1615 1869 2283 2531 2566 236
Size: 1379 254 414 248 35 2523
Fragments arranged by size:
2523 1379 414 254 248 35
BanII G_rGCy'C
Cuts at: 1341 1519 1867 2897 1341
Size: 178 348 1030 3297
Fragments arranged by size:
3297 1030 348 178
BbeI G_GCGC'C
Cuts at: 2287 2535 2287
Size: 248 4605
BbsI GAAGACnn'nnnn_
Cuts at: 638 1790 638
Size: 1152 3701
BbvCI CC'TCA_GC
Cuts at: 1388 1451 1744 1388
Size: 63 293 4497
Fragments arranged by size:
4497 293 63
BciVI GTATCCnnnnn_n'
Cuts at: 2751 3807 4853 2751
Size: 1056 1046 2751
Fragments arranged by size:
2751 1056 1046
BclI T'GATC_A
Cuts at: 2373 2373
Size: 4853
BglI GCCn_nnn'nGGC
Cuts at: 322 1162 322
Size: 840 4013
BglII A'GATC_T
Cuts at: 746 1462 2368 4405 746
Size: 716 906 2037 1194
Fragments arranged by size:
2037 1194 906 716
BlpI GC'TnA_GC
Cuts at: 1965 1965
Size: 4853
Bme1580I G_kGCm'C
Cuts at: 2478 2571 3695 2478
Size: 93 1124 3636
Fragments arranged by size:
3636 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1515 2065 2146 2481 4625 1515
Size: 550 81 335 2144 1743
Fragments arranged by size:
2144 1743 550 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3220 3220
Size: 4853
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 282 332 1096 254
Size: 28 50 764 4011
Fragments arranged by size:
4011 764 50 28
Bpu10I CC'TnA_GC
Cuts at: 1388 1451 1513 1744 1388
Size: 63 62 231 4497
Fragments arranged by size:
4497 231 63 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2029 2908 3622 3920 2029
Size: 879 714 298 2962
Fragments arranged by size:
2962 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4557 4557
Size: 4853
BsaAI yAC'GTr
Cuts at: 2206 2206
Size: 4853
BsaBI GATnn'nnATC
Cuts at: 6 460 2367 4404 6
Size: 454 1907 2037 455
Fragments arranged by size:
2037 1907 455 454
BsaHI Gr'CG_yC
Cuts at: 126 2284 2532 3234 4730 126
Size: 2158 248 702 1496 249
Fragments arranged by size:
2158 1496 702 249 248
BsaWI w'CCGG_w
Cuts at: 1525 2042 2563 3241 3652 3799 4073 1525
Size: 517 521 678 411 147 274 2305
Fragments arranged by size:
2305 678 521 517 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1223 1253 1588 1618 1223
Size: 30 335 30 4458
Fragments arranged by size:
4458 335 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 246 255 1439 246
Size: 9 1184 3660
Fragments arranged by size:
3660 1184 9
BseYI C'CCAG_C
Cuts at: 242 852 1376 1380 1403 1667 1763 2278 3036
Size: 610 524 4 23 264 96 515 758
Cuts at: 3036 3701 4109 242
Size: 665 408 986
Fragments arranged by size:
986 758 665 610 524 515 408 264
96 23 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1669 1669
Size: 4853
BsiEI CG_ry'CG
Cuts at: 104 2395 2441 3671 104
Size: 2291 46 1230 1286
Fragments arranged by size:
2291 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1867 2645 2835 3695 1867
Size: 778 190 860 3025
Fragments arranged by size:
3025 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 133 133
Size: 4853
Bsp1286I G_dGCh'C
Cuts at: 1341 1519 1867 2478 2571 2645 2835 2897 3695
Size: 178 348 611 93 74 190 62 798
Cuts at: 3695 1341
Size: 2499
Fragments arranged by size:
2499 798 611 348 190 178 93 74
62
BspEI T'CCGG_A
Cuts at: 1525 1525
Size: 4853
BspHI T'CATG_A
Cuts at: 3285 3285
Size: 4853
BspMI ACCTGCnnnn'nnnn_
Cuts at: 164 188 219 528 1071 1903 2117 2419 2800
Size: 24 31 309 543 832 214 302 381
Cuts at: 2800 164
Size: 2217
Fragments arranged by size:
2217 832 543 381 309 302 214 31
24
BsrBI CCG'CTC
Cuts at: 50 3145 3199 4683 4848 50
Size: 3095 54 1484 165 55
Fragments arranged by size:
3095 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2765 2765
Size: 4853
BsrFI r'CCGG_y
Cuts at: 2851 3032 2851
Size: 181 4672
BssSI C'ACGA_G
Cuts at: 3124 3832 3124
Size: 708 4145
BstAPI GCAn_nnn'nTGC
Cuts at: 2230 2230
Size: 4853
BstBI TT'CG_AA
Cuts at: 3214 3214
Size: 4853
BstEII G'GTnAC_C
Cuts at: 1547 1547
Size: 4853
BstXI CCAn_nnnn'nTGG
Cuts at: 1000 1465 1000
Size: 465 4388
BstYI r'GATC_y
Cuts at: 1 274 383 461 571 746 1003 1462 1528
Size: 273 109 78 110 175 257 459 66
Cuts at: 1528 1878 2345 2368 2703 2949 3255 3267 3353
Size: 350 467 23 335 246 306 12 86
Cuts at: 3353 4405 1
Size: 1052 449
Fragments arranged by size:
1052 467 459 449 350 335 306 273
257 246 175 110 109 86 78 66
23 12
Bsu36I CC'TnA_GG
Cuts at: 320 330 1816 320
Size: 10 1486 3357
Fragments arranged by size:
3357 1486 10
BtgI C'CryG_G
Cuts at: 224 432 932 224
Size: 208 500 4145
Fragments arranged by size:
4145 500 208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2803 2984 3147 2803
Size: 181 163 4509
Fragments arranged by size:
4509 181 163
BtsI GCAGTG_nn'
Cuts at: 2096 2096
Size: 4853
ClaI AT'CG_AT
Cuts at: 398 398
Size: 4853
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 348 383 348
Size: 35 4818
DraI TTT'AAA
Cuts at: 1852 1852
Size: 4853
DrdI GACnn_nn'nnGTC
Cuts at: 538 1273 2559 3903 538
Size: 735 1286 1344 1488
Fragments arranged by size:
1488 1344 1286 735
EaeI y'GGCC_r
Cuts at: 149 935 1085 1262 2438 2612 3003 3030 4375
Size: 786 150 177 1176 174 391 27 1345
Cuts at: 4375 149
Size: 627
Fragments arranged by size:
1345 1176 786 627 391 177 174 150
27
EagI C'GGCC_G
Cuts at: 2438 2438
Size: 4853
EarI CTCTTCn'nnn_
Cuts at: 1238 1673 2876 3086 1238
Size: 435 1203 210 3005
Fragments arranged by size:
3005 1203 435 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 589 3061 3803 3949 4663 589
Size: 2472 742 146 714 779
Fragments arranged by size:
2472 779 742 714 146
EcoICRI GAG'CTC
Cuts at: 1865 1865
Size: 4853
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 282 332 1096 2678 3110 3463 4125 254
Size: 28 50 764 1582 432 353 662 982
Fragments arranged by size:
1582 982 764 662 432 353 50 28
EcoNI CCTnn'n_nnAGG
Cuts at: 1391 1421 1391
Size: 30 4823
EcoO109I rG'GnC_Cy
Cuts at: 315 352 763 1384 1429 1772 1992 315
Size: 37 411 621 45 343 220 3176
Fragments arranged by size:
3176 621 411 343 220 45 37
EcoRI G'AATT_C
Cuts at: 481 1857 481
Size: 1376 3477
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 603 2872 603
Size: 2269 2584
FspI TGC'GCA
Cuts at: 2634 2634
Size: 4853
HaeII r_GCGC'y
Cuts at: 1318 2287 2535 3765 4536 1318
Size: 969 248 1230 771 1635
Fragments arranged by size:
1635 1230 969 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 330 362 368 400 1108 1140 1178 1210 2695
Size: 32 6 32 708 32 38 32 1485
Cuts at: 2695 2725 2727 2757 330
Size: 30 2 30 2426
Fragments arranged by size:
2426 1485 708 38 32 32 32 32
30 30 6 2
HincII GTy'rAC
Cuts at: 684 1892 684
Size: 1208 3645
KasI G'GCGC_C
Cuts at: 2283 2531 2283
Size: 248 4605
KpnI G_GTAC'C
Cuts at: 1873 1873
Size: 4853
MluI A'CGCG_T
Cuts at: 4286 4286
Size: 4853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1031 1778 3612 3796 1031
Size: 747 1834 184 2088
Fragments arranged by size:
2088 1834 747 184
MscI TGG'CCA
Cuts at: 151 937 1087 2614 151
Size: 786 150 1527 2390
Fragments arranged by size:
2390 1527 786 150
MslI CAynn'nnrTG
Cuts at: 922 2842 2969 922
Size: 1920 127 2806
Fragments arranged by size:
2806 1920 127
MspA1I CmG'CkG
Cuts at: 1121 1224 1257 1380 1964 2278 2638 3422 3667
Size: 103 33 123 584 314 360 784 245
Cuts at: 3667 1121
Size: 2307
Fragments arranged by size:
2307 784 584 360 314 245 123 103
33
NaeI GCC'GGC
Cuts at: 3034 3034
Size: 4853
NarI GG'CG_CC
Cuts at: 2284 2532 2284
Size: 248 4605
NcoI C'CATG_G
Cuts at: 224 432 932 224
Size: 208 500 4145
Fragments arranged by size:
4145 500 208
NgoMIV G'CCGG_C
Cuts at: 3032 3032
Size: 4853
NspI r_CATG'y
Cuts at: 537 610 862 1240 1906 2406 4009 537
Size: 73 252 378 666 500 1603 1381
Fragments arranged by size:
1603 1381 666 500 378 252 73
PasI CC'CwG_GG
Cuts at: 624 722 759 1366 624
Size: 98 37 607 4111
Fragments arranged by size:
4111 607 98 37
PciI A'CATG_T
Cuts at: 4005 4005
Size: 4853
PflMI CCAn_nnn'nTGG
Cuts at: 249 759 249
Size: 510 4343
PfoI T'CCnGG_A
Cuts at: 270 908 270
Size: 638 4215
PmeI GTTT'AAAC
Cuts at: 1852 1852
Size: 4853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 4821
PpuMI rG'GwC_Cy
Cuts at: 352 763 1772 352
Size: 411 1009 3433
Fragments arranged by size:
3433 1009 411
PstI C_TGCA'G
Cuts at: 662 715 1900 2585 662
Size: 53 1185 685 2930
Fragments arranged by size:
2930 1185 685 53
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4853
PvuII CAG'CTG
Cuts at: 1121 1257 1380 2278 2638 1121
Size: 136 123 898 360 3336
Fragments arranged by size:
3336 898 360 136 123
RsrII CG'GwC_CG
Cuts at: 3048 3048
Size: 4853
SacI G_AGCT'C
Cuts at: 1867 1867
Size: 4853
SalI G'TCGA_C
Cuts at: 1890 1890
Size: 4853
SanDI GG'GwC_CC
Cuts at: 1772 1772
Size: 4853
SapI GCTCTTCn'nnn_
Cuts at: 2876 3086 2876
Size: 210 4643
SbfI CC_TGCA'GG
Cuts at: 1900 1900
Size: 4853
ScaI AGT'ACT
Cuts at: 4370 4370
Size: 4853
SfcI C'TryA_G
Cuts at: 33 612 658 711 1896 2581 3549 3740 33
Size: 579 46 53 1185 685 968 191 1146
Fragments arranged by size:
1185 1146 968 685 579 191 53 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 322 322
Size: 4853
SfoI GGC'GCC
Cuts at: 2285 2533 2285
Size: 248 4605
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4853
SmaI CCC'GGG
Cuts at: 1573 1875 1573
Size: 302 4551
SmlI C'TyrA_G
Cuts at: 2008 2923 3637 3899 2008
Size: 915 714 262 2962
Fragments arranged by size:
2962 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4853
SphI G_CATG'C
Cuts at: 610 1906 2406 610
Size: 1296 500 3057
Fragments arranged by size:
3057 1296 500
StuI AGG'CCT
Cuts at: 961 1603 961
Size: 642 4211
StyI C'CwwG_G
Cuts at: 224 432 932 1333 1793 1987 2341 224
Size: 208 500 401 460 194 354 2736
Fragments arranged by size:
2736 500 460 401 354 208 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 714 1101 2436 2569 3028 3237 714
Size: 387 1335 133 459 209 2330
Fragments arranged by size:
2330 1335 459 387 209 133
TatI w'GTAC_w
Cuts at: 4368 4368
Size: 4853
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1223 1255 1764 1796 4370 4402 1223
Size: 32 509 32 2574 32 1674
Fragments arranged by size:
2574 1674 509 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2650 2650
Size: 4853
XbaI T'CTAG_A
Cuts at: 1884 1884
Size: 4853
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 387 1177 2348 387
Size: 790 1171 2892
Fragments arranged by size:
2892 1171 790
XmaI C'CCGG_G
Cuts at: 1571 1873 1571
Size: 302 4551
XmnI GAAnn'nnTTC
Cuts at: 427 427
Size: 4853
ZraI GAC'GTC
Cuts at: 127 127
Size: 4853
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsrGI BssHII BstZ17I DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI