Restriction map for
pF1KB4920
(Circular) (Six-base) MAPSORT of: pF1KB4920.seq Check: 6289 from: 1 to: 5396
pF1KB4920 5396 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 252 638 2434 252
Size: 386 1796 3214
Fragments arranged by size:
3214 1796 386
Acc65I G'GTAC_C
Cuts at: 2412 2412
Size: 5396
AclI AA'CG_TT
Cuts at: 5236 5236
Size: 5396
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1389 1473 1557 1663 1893 3221 3653 4006 4668
Size: 84 84 106 230 1328 432 353 662
Cuts at: 4668 1389
Size: 2117
Fragments arranged by size:
2117 1328 662 432 353 230 106 84
84
AflII C'TTAA_G
Cuts at: 331 331
Size: 5396
AflIII A'CryG_T
Cuts at: 2361 2748 4548 4829 2361
Size: 387 1800 281 2928
Fragments arranged by size:
2928 1800 387 281
AhdI GACnn_n'nnGTC
Cuts at: 1658 1658
Size: 5396
AleI CACnn'nnGTG
Cuts at: 1391 1391
Size: 5396
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 120 152 120
Size: 32 5364
AlwNI CAG_nnn'CTG
Cuts at: 2701 4139 2701
Size: 1438 3958
ApaLI G'TGCA_C
Cuts at: 1415 1499 2005 4234 1415
Size: 84 506 2229 2577
Fragments arranged by size:
2577 2229 506 84
ApoI r'AATT_y
Cuts at: 258 267 368 800 965 1234 1439 1775 2279
Size: 9 101 432 165 269 205 336 504
Cuts at: 2279 2400 4749 5005 258
Size: 121 2349 256 649
Fragments arranged by size:
2349 649 504 432 336 269 256 205
165 121 101 9
AseI AT'TA_AT
Cuts at: 20 1595 20
Size: 1575 3821
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5396
AvaI C'yCGr_G
Cuts at: 2416 2416
Size: 5396
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1254 1287 1254
Size: 33 5363
BamHI G'GATC_C
Cuts at: 2421 2421
Size: 5396
BanI G'GyrC_C
Cuts at: 2374 2412 2826 3074 3109 2374
Size: 38 414 248 35 4661
Fragments arranged by size:
4661 414 248 38 35
BanII G_rGCy'C
Cuts at: 326 2410 3440 326
Size: 2084 1030 2282
Fragments arranged by size:
2282 2084 1030
BbeI G_GCGC'C
Cuts at: 2830 3078 2830
Size: 248 5148
BbsI GAAGACnn'nnnn_
Cuts at: 557 914 1657 557
Size: 357 743 4296
Fragments arranged by size:
4296 743 357
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 308 342 308
Size: 34 5362
BciVI GTATCCnnnnn_n'
Cuts at: 455 3294 4350 5396 455
Size: 2839 1056 1046 455
Fragments arranged by size:
2839 1056 1046 455
BclI T'GATC_A
Cuts at: 2916 2916
Size: 5396
BfrBI ATG'CAT
Cuts at: 2305 2305
Size: 5396
BglII A'GATC_T
Cuts at: 2911 4948 2911
Size: 2037 3359
BlpI GC'TnA_GC
Cuts at: 2508 2508
Size: 5396
Bme1580I G_kGCm'C
Cuts at: 1419 1503 2009 3021 3114 4238 1419
Size: 84 506 1012 93 1124 2577
Fragments arranged by size:
2577 1124 1012 506 93 84
BmrI ACTGGGnnnn_n'
Cuts at: 435 666 1709 2608 2689 3024 5168 435
Size: 231 1043 899 81 335 2144 663
Fragments arranged by size:
2144 1043 899 663 335 231 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 372 3763 372
Size: 3391 2005
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 836 1217 1385 1469 1553 1637 1805 1889
Size: 427 381 168 84 84 84 168 84
Cuts at: 1889 1973 2141 2225 409
Size: 84 168 84 3580
Fragments arranged by size:
3580 427 381 168 168 168 84 84
84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 2572 3451 4165 4463 102
Size: 2470 879 714 298 1035
Fragments arranged by size:
2470 1035 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 238 336 2205 5100 238
Size: 98 1869 2895 534
Fragments arranged by size:
2895 1869 534 98
BsaAI yAC'GTr
Cuts at: 2749 2749
Size: 5396
BsaBI GATnn'nnATC
Cuts at: 6 2910 4947 6
Size: 2904 2037 455
Fragments arranged by size:
2904 2037 455
BsaHI Gr'CG_yC
Cuts at: 2827 3075 3777 5273 2827
Size: 248 702 1496 2950
Fragments arranged by size:
2950 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2585 3106 3784 4195 4342 4616 2585
Size: 521 678 411 147 274 3365
Fragments arranged by size:
3365 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 850 880 850
Size: 30 5366
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 503 503
Size: 5396
BseYI C'CCAG_C
Cuts at: 2821 3579 4244 4652 2821
Size: 758 665 408 3565
Fragments arranged by size:
3565 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 697
Size: 5396
BsiEI CG_ry'CG
Cuts at: 104 2938 2984 4214 104
Size: 2834 46 1230 1286
Fragments arranged by size:
2834 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 326 1419 1503 2009 2410 3188 3378 4238 326
Size: 1093 84 506 401 778 190 860 1484
Fragments arranged by size:
1484 1093 860 778 506 401 190 84
BsmI GAATG_Cn'
Cuts at: 1077 1361 1387 1990 1077
Size: 284 26 603 4483
Fragments arranged by size:
4483 603 284 26
Bsp1286I G_dGCh'C
Cuts at: 326 1419 1503 2009 2410 3021 3114 3188 3378
Size: 1093 84 506 401 611 93 74 190
Cuts at: 3378 3440 4238 326
Size: 62 798 1484
Fragments arranged by size:
1484 1093 798 611 506 401 190 93
84 74 62
BspHI T'CATG_A
Cuts at: 3828 3828
Size: 5396
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2446 2660 2962 3343 2446
Size: 214 302 381 4499
Fragments arranged by size:
4499 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3688 3742 5226 5391 50
Size: 3638 54 1484 165 55
Fragments arranged by size:
3638 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1644 1728 3308 1644
Size: 84 1580 3732
Fragments arranged by size:
3732 1580 84
BsrFI r'CCGG_y
Cuts at: 3394 3575 3394
Size: 181 5215
BsrGI T'GTAC_A
Cuts at: 746 1092 1176 746
Size: 346 84 4966
Fragments arranged by size:
4966 346 84
BssSI C'ACGA_G
Cuts at: 3667 4375 3667
Size: 708 4688
BstAPI GCAn_nnn'nTGC
Cuts at: 2773 2773
Size: 5396
BstBI TT'CG_AA
Cuts at: 3757 3757
Size: 5396
BstEII G'GTnAC_C
Cuts at: 600 600
Size: 5396
BstXI CCAn_nnnn'nTGG
Cuts at: 1393 1393
Size: 5396
BstYI r'GATC_y
Cuts at: 1 2421 2888 2911 3246 3492 3798 3810 3896
Size: 2420 467 23 335 246 306 12 86
Cuts at: 3896 4948 1
Size: 1052 449
Fragments arranged by size:
2420 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 781 781
Size: 5396
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3346 3527 3690 3346
Size: 181 163 5052
Fragments arranged by size:
5052 181 163
BtsI GCAGTG_nn'
Cuts at: 473 1980 2639 473
Size: 1507 659 3230
Fragments arranged by size:
3230 1507 659
ClaI AT'CG_AT
Cuts at: 613 613
Size: 5396
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1626 1661 2046 2081 1626
Size: 35 385 35 4941
Fragments arranged by size:
4941 385 35 35
DraI TTT'AAA
Cuts at: 2052 2395 2052
Size: 343 5053
DrdI GACnn_nn'nnGTC
Cuts at: 3102 4446 3102
Size: 1344 4052
EaeI y'GGCC_r
Cuts at: 2981 3155 3546 3573 4918 2981
Size: 174 391 27 1345 3459
Fragments arranged by size:
3459 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2981 2981
Size: 5396
EarI CTCTTCn'nnn_
Cuts at: 497 3419 3629 497
Size: 2922 210 2264
Fragments arranged by size:
2922 2264 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3604 4346 4492 5206 3604
Size: 742 146 714 3794
Fragments arranged by size:
3794 742 714 146
EcoICRI GAG'CTC
Cuts at: 324 2408 324
Size: 2084 3312
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 836 1217 1385 1389 1469 1473 1553 1557
Size: 427 381 168 4 80 4 80 4
Cuts at: 1557 1637 1663 1805 1889 1893 1973 2141 2225
Size: 80 26 142 84 4 80 168 84
Cuts at: 2225 3221 3653 4006 4668 409
Size: 996 432 353 662 1137
Fragments arranged by size:
1137 996 662 432 427 381 353 168
168 142 84 84 80 80 80 80
26 4 4 4 4
EcoO109I rG'GnC_Cy
Cuts at: 811 2535 811
Size: 1724 3672
EcoRI G'AATT_C
Cuts at: 1439 1775 2279 2400 1439
Size: 336 504 121 4435
Fragments arranged by size:
4435 504 336 121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1054 3415 1054
Size: 2361 3035
FspI TGC'GCA
Cuts at: 3177 3177
Size: 5396
HaeII r_GCGC'y
Cuts at: 2830 3078 4308 5079 2830
Size: 248 1230 771 3147
Fragments arranged by size:
3147 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 850 882 1584 1616 1644 1676 1908 1940 3238
Size: 32 702 32 28 32 232 32 1298
Cuts at: 3238 3268 3270 3300 850
Size: 30 2 30 2946
Fragments arranged by size:
2946 1298 702 232 32 32 32 32
30 30 28 2
HincII GTy'rAC
Cuts at: 253 313 2435 253
Size: 60 2122 3214
Fragments arranged by size:
3214 2122 60
HindIII A'AGCT_T
Cuts at: 2156 2156
Size: 5396
KasI G'GCGC_C
Cuts at: 2826 3074 2826
Size: 248 5148
KpnI G_GTAC'C
Cuts at: 2416 2416
Size: 5396
MluI A'CGCG_T
Cuts at: 4829 4829
Size: 5396
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1711 4155 4339 1711
Size: 2444 184 2768
Fragments arranged by size:
2768 2444 184
MscI TGG'CCA
Cuts at: 3157 3157
Size: 5396
MslI CAynn'nnrTG
Cuts at: 974 1380 1391 1548 1716 1968 2360 2372 2387
Size: 406 11 157 168 252 392 12 15
Cuts at: 2387 3385 3512 974
Size: 998 127 2858
Fragments arranged by size:
2858 998 406 392 252 168 157 127
15 12 11
MspA1I CmG'CkG
Cuts at: 2507 2821 3181 3965 4210 2507
Size: 314 360 784 245 3693
Fragments arranged by size:
3693 784 360 314 245
NaeI GCC'GGC
Cuts at: 3577 3577
Size: 5396
NarI GG'CG_CC
Cuts at: 2827 3075 2827
Size: 248 5148
NcoI C'CATG_G
Cuts at: 781 781
Size: 5396
NdeI CA'TA_TG
Cuts at: 915 1167 1377 915
Size: 252 210 4934
Fragments arranged by size:
4934 252 210
NgoMIV G'CCGG_C
Cuts at: 3575 3575
Size: 5396
NsiI A_TGCA'T
Cuts at: 2307 2307
Size: 5396
NspI r_CATG'y
Cuts at: 571 848 2261 2309 2449 2949 4552 571
Size: 277 1413 48 140 500 1603 1415
Fragments arranged by size:
1603 1415 1413 500 277 140 48
PciI A'CATG_T
Cuts at: 4548 4548
Size: 5396
PfoI T'CCnGG_A
Cuts at: 1194 1194
Size: 5396
PmeI GTTT'AAAC
Cuts at: 2395 2395
Size: 5396
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 3842
Size: 32 5364
PshAI GACnn'nnGTC
Cuts at: 1490 1490
Size: 5396
PsiI TTA'TAA
Cuts at: 1799 1799
Size: 5396
PstI C_TGCA'G
Cuts at: 2443 3128 2443
Size: 685 4711
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5396
PvuII CAG'CTG
Cuts at: 2821 3181 2821
Size: 360 5036
RsrII CG'GwC_CG
Cuts at: 3591 3591
Size: 5396
SacI G_AGCT'C
Cuts at: 326 2410 326
Size: 2084 3312
SalI G'TCGA_C
Cuts at: 251 2433 251
Size: 2182 3214
SapI GCTCTTCn'nnn_
Cuts at: 3419 3629 3419
Size: 210 5186
SbfI CC_TGCA'GG
Cuts at: 2443 2443
Size: 5396
ScaI AGT'ACT
Cuts at: 1752 4913 1752
Size: 3161 2235
SfcI C'TryA_G
Cuts at: 33 635 1025 2439 3124 4092 4283 33
Size: 602 390 1414 685 968 191 1146
Fragments arranged by size:
1414 1146 968 685 602 390 191
SfoI GGC'GCC
Cuts at: 2828 3076 2828
Size: 248 5148
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5396
SmaI CCC'GGG
Cuts at: 2418 2418
Size: 5396
SmlI C'TyrA_G
Cuts at: 117 331 2551 3466 4180 4442 117
Size: 214 2220 915 714 262 1071
Fragments arranged by size:
2220 1071 915 714 262 214
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5396
SphI G_CATG'C
Cuts at: 2449 2949 2449
Size: 500 4896
StyI C'CwwG_G
Cuts at: 781 2530 2884 781
Size: 1749 354 3293
Fragments arranged by size:
3293 1749 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 661 2347 2979 3112 3571 3780 661
Size: 1686 632 133 459 209 2277
Fragments arranged by size:
2277 1686 632 459 209 133
TatI w'GTAC_w
Cuts at: 746 847 1092 1176 1666 1750 2338 4911 746
Size: 101 245 84 490 84 588 2573 1231
Fragments arranged by size:
2573 1231 588 490 245 101 84 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 728 760 4913 4945 728
Size: 32 4153 32 1179
Fragments arranged by size:
4153 1179 32 32
Tth111I GACn'n_nGTC
Cuts at: 3193 3193
Size: 5396
XbaI T'CTAG_A
Cuts at: 498 2427 498
Size: 1929 3467
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2891 2891
Size: 5396
XmaI C'CCGG_G
Cuts at: 2416 2416
Size: 5396
XmnI GAAnn'nnTTC
Cuts at: 1486 1654 1779 1906 1990 2158 1486
Size: 168 125 127 84 168 4724
Fragments arranged by size:
4724 168 168 127 125 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AlfI ApaI AscI AvrII
BbvCI BglI BmgBI BmtI Bpu10I BsiWI BsmBI BspEI
BssHII BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NheI NotI NruI PacI PasI PflMI
PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI