Restriction map for
pF1KB4917
(Circular) (Six-base) MAPSORT of: pF1KB4917.seq Check: 110 from: 1 to: 4082
pF1KB4917 4082 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 187 460 187
Size: 273 3809
AccI GT'mk_AC
Cuts at: 1022 1120 1022
Size: 98 3984
Acc65I G'GTAC_C
Cuts at: 1098 1098
Size: 4082
AclI AA'CG_TT
Cuts at: 3922 3922
Size: 4082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 859 982 1907 2339 3086 3354 508
Size: 351 123 925 432 747 268 1236
Fragments arranged by size:
1236 925 747 432 351 268 123
AflIII A'CryG_T
Cuts at: 785 1434 2538 3515 785
Size: 649 1104 977 1352
Fragments arranged by size:
1352 1104 977 649
AgeI A'CCGG_T
Cuts at: 2470 2470
Size: 4082
AleI CACnn'nnGTG
Cuts at: 181 181
Size: 4082
AlwNI CAG_nnn'CTG
Cuts at: 408 1387 2954 408
Size: 979 1567 1536
Fragments arranged by size:
1567 1536 979
ApaI G_GGCC'C
Cuts at: 949 949
Size: 4082
ApaLI G'TGCA_C
Cuts at: 2852 2852
Size: 4082
ApoI r'AATT_y
Cuts at: 1086 3435 3691 1086
Size: 2349 256 1477
Fragments arranged by size:
2349 1477 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4082
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
AvaI C'yCGr_G
Cuts at: 340 676 1102 340
Size: 336 426 3320
Fragments arranged by size:
3320 426 336
BamHI G'GATC_C
Cuts at: 908 1107 908
Size: 199 3883
BanI G'GyrC_C
Cuts at: 448 571 967 1098 1512 1760 1795 448
Size: 123 396 131 414 248 35 2735
Fragments arranged by size:
2735 414 396 248 131 123 35
BanII G_rGCy'C
Cuts at: 949 1096 2126 949
Size: 147 1030 2905
Fragments arranged by size:
2905 1030 147
BbeI G_GCGC'C
Cuts at: 971 1516 1764 971
Size: 545 248 3289
Fragments arranged by size:
3289 545 248
BbsI GAAGACnn'nnnn_
Cuts at: 862 862
Size: 4082
BciVI GTATCCnnnnn_n'
Cuts at: 1980 2741 4082 1980
Size: 761 1341 1980
Fragments arranged by size:
1980 1341 761
BclI T'GATC_A
Cuts at: 1602 1602
Size: 4082
BglI GCCn_nnn'nGGC
Cuts at: 829 829
Size: 4082
BglII A'GATC_T
Cuts at: 1597 3634 1597
Size: 2037 2045
BlpI GC'TnA_GC
Cuts at: 1194 1194
Size: 4082
Bme1580I G_kGCm'C
Cuts at: 576 949 1707 1800 2856 576
Size: 373 758 93 1056 1802
Fragments arranged by size:
1802 1056 758 373 93
BmrI ACTGGGnnnn_n'
Cuts at: 1294 1375 1710 3854 1294
Size: 81 335 2144 1522
Fragments arranged by size:
2144 1522 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 966 2449 966
Size: 1483 2599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 850 578
Size: 272 3810
Bpu10I CC'TnA_GC
Cuts at: 691 691
Size: 4082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1258 2137 2629 2927 1258
Size: 879 492 298 2413
Fragments arranged by size:
2413 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 667 3786 667
Size: 3119 963
BsaAI yAC'GTr
Cuts at: 786 1435 786
Size: 649 3433
BsaBI GATnn'nnATC
Cuts at: 6 1596 3633 6
Size: 1590 2037 455
Fragments arranged by size:
2037 1590 455
BsaHI Gr'CG_yC
Cuts at: 760 884 968 1513 1761 2463 3959 760
Size: 124 84 545 248 702 1496 883
Fragments arranged by size:
1496 883 702 545 248 124 84
BsaWI w'CCGG_w
Cuts at: 1271 1792 2470 2744 2891 3302 1271
Size: 521 678 274 147 411 2051
Fragments arranged by size:
2051 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 302 332 302
Size: 30 4052
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 781 781
Size: 4082
BseYI C'CCAG_C
Cuts at: 575 620 747 811 1050 1507 2265 2842 3338
Size: 45 127 64 239 457 758 577 496
Cuts at: 3338 575
Size: 1319
Fragments arranged by size:
1319 758 577 496 457 239 127 64
45
BsiEI CG_ry'CG
Cuts at: 104 1624 1670 2878 104
Size: 1520 46 1208 1308
Fragments arranged by size:
1520 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1096 1874 2064 2856 1096
Size: 778 190 792 2322
Fragments arranged by size:
2322 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 576 949 1096 1707 1800 1874 2064 2126 2856
Size: 373 147 611 93 74 190 62 730
Cuts at: 2856 576
Size: 1802
Fragments arranged by size:
1802 730 611 373 190 147 93 74
62
BspEI T'CCGG_A
Cuts at: 3302 3302
Size: 4082
BspHI T'CATG_A
Cuts at: 3258 3258
Size: 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 187 460 644 1132 1346 1648 2029 187
Size: 273 184 488 214 302 381 2240
Fragments arranged by size:
2240 488 381 302 273 214 184
BsrBI CCG'CTC
Cuts at: 50 281 726 2374 2428 3912 4077 50
Size: 231 445 1648 54 1484 165 55
Fragments arranged by size:
1648 1484 445 231 165 55 54
BsrDI GCAATG_nn'
Cuts at: 515 653 1994 515
Size: 138 1341 2603
Fragments arranged by size:
2603 1341 138
BsrFI r'CCGG_y
Cuts at: 658 799 2080 2261 2470 658
Size: 141 1281 181 209 2270
Fragments arranged by size:
2270 1281 209 181 141
BsrGI T'GTAC_A
Cuts at: 242 242
Size: 4082
BssHII G'CGCG_C
Cuts at: 980 980
Size: 4082
BssSI C'ACGA_G
Cuts at: 246 940 2353 2711 246
Size: 694 1413 358 1617
Fragments arranged by size:
1617 1413 694 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1459 1459
Size: 4082
BstBI TT'CG_AA
Cuts at: 2443 2443
Size: 4082
BstXI CCAn_nnnn'nTGG
Cuts at: 198 357 477 198
Size: 159 120 3803
Fragments arranged by size:
3803 159 120
BstYI r'GATC_y
Cuts at: 1 491 533 908 929 1107 1574 1597 1932
Size: 490 42 375 21 178 467 23 335
Cuts at: 1932 2178 3190 3276 3288 3634 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 490 467 449 375 346 335 246
178 86 42 23 21 12
BtgI C'CryG_G
Cuts at: 107 476 611 648 107
Size: 369 135 37 3541
Fragments arranged by size:
3541 369 135 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 141 975 993 1020 2032 2213 2376 141
Size: 834 18 27 1012 181 163 1847
Fragments arranged by size:
1847 1012 834 181 163 27 18
BtsI GCAGTG_nn'
Cuts at: 1325 1325
Size: 4082
DraI TTT'AAA
Cuts at: 1081 1081
Size: 4082
DrdI GACnn_nn'nnGTC
Cuts at: 1788 2646 1788
Size: 858 3224
EaeI y'GGCC_r
Cuts at: 200 231 614 797 830 1667 1841 2232 2259
Size: 31 383 183 33 837 174 391 27
Cuts at: 2259 3604 200
Size: 1345 678
Fragments arranged by size:
1345 837 678 391 383 183 174 33
31 27
EagI C'GGCC_G
Cuts at: 1667 1667
Size: 4082
EarI CTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2290 2600 2746 3892 2290
Size: 310 146 1146 2480
Fragments arranged by size:
2480 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1094 1094
Size: 4082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 578 850 859 982 1907 2339 3086 3354
Size: 70 272 9 123 925 432 747 268
Cuts at: 3354 508
Size: 1236
Fragments arranged by size:
1236 925 747 432 272 268 123 70
9
EcoO109I rG'GnC_Cy
Cuts at: 945 946 1065 1221 945
Size: 1 119 156 3806
Fragments arranged by size:
3806 156 119 1
EcoRI G'AATT_C
Cuts at: 1086 1086
Size: 4082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 4082
FspI TGC'GCA
Cuts at: 1863 1863
Size: 4082
HaeII r_GCGC'y
Cuts at: 971 1516 1764 2786 3765 971
Size: 545 248 1022 979 1288
Fragments arranged by size:
1288 1022 979 545 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 371 403 757 789 886 918 1924 1954 1956
Size: 32 354 32 97 32 1006 30 2
Cuts at: 1956 1986 371
Size: 30 2467
Fragments arranged by size:
2467 1006 354 97 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 837 888 1121 837
Size: 51 233 3798
Fragments arranged by size:
3798 233 51
KasI G'GCGC_C
Cuts at: 967 1512 1760 967
Size: 545 248 3289
Fragments arranged by size:
3289 545 248
KpnI G_GTAC'C
Cuts at: 1102 1102
Size: 4082
MluI A'CGCG_T
Cuts at: 3515 3515
Size: 4082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 281 402 2753 2937 281
Size: 121 2351 184 1426
Fragments arranged by size:
2351 1426 184 121
MscI TGG'CCA
Cuts at: 202 1843 202
Size: 1641 2441
MslI CAynn'nnrTG
Cuts at: 181 250 475 481 2071 2198 181
Size: 69 225 6 1590 127 2065
Fragments arranged by size:
2065 1590 225 127 69 6
MspA1I CmG'CkG
Cuts at: 498 598 624 747 826 1193 1507 1867 2880
Size: 100 26 123 79 367 314 360 1013
Cuts at: 2880 3125 498
Size: 245 1455
Fragments arranged by size:
1455 1013 367 360 314 245 123 100
79 26
NaeI GCC'GGC
Cuts at: 801 2263 801
Size: 1462 2620
NarI GG'CG_CC
Cuts at: 968 1513 1761 968
Size: 545 248 3289
Fragments arranged by size:
3289 545 248
NcoI C'CATG_G
Cuts at: 107 476 107
Size: 369 3713
NgoMIV G'CCGG_C
Cuts at: 799 2261 799
Size: 1462 2620
NspI r_CATG'y
Cuts at: 255 303 1135 1635 2542 255
Size: 48 832 500 907 1795
Fragments arranged by size:
1795 907 832 500 48
PasI CC'CwG_GG
Cuts at: 546 564 546
Size: 18 4064
PciI A'CATG_T
Cuts at: 2538 2538
Size: 4082
PfoI T'CCnGG_A
Cuts at: 911 911
Size: 4082
PmeI GTTT'AAAC
Cuts at: 1081 1081
Size: 4082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 4050
PpuMI rG'GwC_Cy
Cuts at: 1065 1065
Size: 4082
PshAI GACnn'nnGTC
Cuts at: 331 331
Size: 4082
PspOMI G'GGCC_C
Cuts at: 945 945
Size: 4082
PstI C_TGCA'G
Cuts at: 218 503 1064 1129 1814 218
Size: 285 561 65 685 2486
Fragments arranged by size:
2486 685 561 285 65
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4082
PvuII CAG'CTG
Cuts at: 498 598 624 747 1507 1867 498
Size: 100 26 123 760 360 2713
Fragments arranged by size:
2713 760 360 123 100 26
RsrII CG'GwC_CG
Cuts at: 2277 2277
Size: 4082
SacI G_AGCT'C
Cuts at: 1096 1096
Size: 4082
SalI G'TCGA_C
Cuts at: 1119 1119
Size: 4082
SapI GCTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
SbfI CC_TGCA'GG
Cuts at: 1129 1129
Size: 4082
ScaI AGT'ACT
Cuts at: 3599 3599
Size: 4082
SfcI C'TryA_G
Cuts at: 33 214 499 1060 1125 1810 2803 2994 33
Size: 181 285 561 65 685 993 191 1121
Fragments arranged by size:
1121 993 685 561 285 191 181 65
SfoI GGC'GCC
Cuts at: 969 1514 1762 969
Size: 545 248 3289
Fragments arranged by size:
3289 545 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
SmaI CCC'GGG
Cuts at: 1104 1104
Size: 4082
SmlI C'TyrA_G
Cuts at: 1237 2152 2644 2906 1237
Size: 915 492 262 2413
Fragments arranged by size:
2413 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4082
SphI G_CATG'C
Cuts at: 1135 1635 1135
Size: 500 3582
StyI C'CwwG_G
Cuts at: 107 476 1216 1570 107
Size: 369 740 354 2619
Fragments arranged by size:
2619 740 369 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 634 1665 1798 2257 2466 634
Size: 1031 133 459 209 2250
Fragments arranged by size:
2250 1031 459 209 133
TatI w'GTAC_w
Cuts at: 242 716 3597 242
Size: 474 2881 727
Fragments arranged by size:
2881 727 474
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 302 334 3599 3631 302
Size: 32 3265 32 753
Fragments arranged by size:
3265 753 32 32
Tth111I GACn'n_nGTC
Cuts at: 1879 1879
Size: 4082
XbaI T'CTAG_A
Cuts at: 1113 1113
Size: 4082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1577 1577
Size: 4082
XmaI C'CCGG_G
Cuts at: 1102 1102
Size: 4082
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI