Restriction map for
pF1KB4911
(Circular) (Six-base) MAPSORT of: pF1KB4911.seq Check: 5139 from: 1 to: 3695
pF1KB4911 3695 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 733 733
Size: 3695
Acc65I G'GTAC_C
Cuts at: 711 711
Size: 3695
AclI AA'CG_TT
Cuts at: 3535 3535
Size: 3695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 1520 1952 2699 2967 650
Size: 870 432 747 268 1378
Fragments arranged by size:
1378 870 747 432 268
AflIII A'CryG_T
Cuts at: 1047 2151 3128 1047
Size: 1104 977 1614
Fragments arranged by size:
1614 1104 977
AgeI A'CCGG_T
Cuts at: 2083 2083
Size: 3695
AlwNI CAG_nnn'CTG
Cuts at: 1000 2567 1000
Size: 1567 2128
ApaLI G'TGCA_C
Cuts at: 2465 2465
Size: 3695
ApoI r'AATT_y
Cuts at: 271 602 699 3048 3304 271
Size: 331 97 2349 256 662
Fragments arranged by size:
2349 662 331 256 97
AseI AT'TA_AT
Cuts at: 20 20
Size: 3695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
AvaI C'yCGr_G
Cuts at: 139 715 139
Size: 576 3119
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 137 170 137
Size: 33 3662
BamHI G'GATC_C
Cuts at: 720 720
Size: 3695
BanI G'GyrC_C
Cuts at: 711 1125 1373 1408 711
Size: 414 248 35 2998
Fragments arranged by size:
2998 414 248 35
BanII G_rGCy'C
Cuts at: 125 709 1739 125
Size: 584 1030 2081
Fragments arranged by size:
2081 1030 584
BbeI G_GCGC'C
Cuts at: 1129 1377 1129
Size: 248 3447
BbsI GAAGACnn'nnnn_
Cuts at: 194 663 194
Size: 469 3226
BbvCI CC'TCA_GC
Cuts at: 115 115
Size: 3695
BciVI GTATCCnnnnn_n'
Cuts at: 138 279 1593 2354 3695 138
Size: 141 1314 761 1341 138
Fragments arranged by size:
1341 1314 761 141 138
BclI T'GATC_A
Cuts at: 1215 1215
Size: 3695
BglII A'GATC_T
Cuts at: 1210 3247 1210
Size: 2037 1658
BlpI GC'TnA_GC
Cuts at: 807 807
Size: 3695
Bme1580I G_kGCm'C
Cuts at: 1320 1413 2469 1320
Size: 93 1056 2546
Fragments arranged by size:
2546 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 907 988 1323 3467 907
Size: 81 335 2144 1135
Fragments arranged by size:
2144 1135 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 2062
Size: 3695
Bpu10I CC'TnA_GC
Cuts at: 115 315 115
Size: 200 3495
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 1750 2242 2540 871
Size: 879 492 298 2026
Fragments arranged by size:
2026 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 538 3399 538
Size: 2861 834
BsaAI yAC'GTr
Cuts at: 1048 1048
Size: 3695
BsaBI GATnn'nnATC
Cuts at: 6 1209 3246 6
Size: 1203 2037 455
Fragments arranged by size:
2037 1203 455
BsaHI Gr'CG_yC
Cuts at: 617 1126 1374 2076 3572 617
Size: 509 248 702 1496 740
Fragments arranged by size:
1496 740 702 509 248
BsaWI w'CCGG_w
Cuts at: 884 1405 2083 2357 2504 2915 884
Size: 521 678 274 147 411 1664
Fragments arranged by size:
1664 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1120 1878 2455 2951 1120
Size: 758 577 496 1864
Fragments arranged by size:
1864 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1237 1283 2491 104
Size: 1133 46 1208 1308
Fragments arranged by size:
1308 1208 1133 46
BsiHKAI G_wGCw'C
Cuts at: 125 709 1487 1677 2469 125
Size: 584 778 190 792 1351
Fragments arranged by size:
1351 792 778 584 190
Bsp1286I G_dGCh'C
Cuts at: 125 709 1320 1413 1487 1677 1739 2469 125
Size: 584 611 93 74 190 62 730 1351
Fragments arranged by size:
1351 730 611 584 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2915 2915
Size: 3695
BspHI T'CATG_A
Cuts at: 2871 2871
Size: 3695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 632 745 959 1261 1642 632
Size: 113 214 302 381 2685
Fragments arranged by size:
2685 381 302 214 113
BsrBI CCG'CTC
Cuts at: 50 1987 2041 3525 3690 50
Size: 1937 54 1484 165 55
Fragments arranged by size:
1937 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 449 1607 449
Size: 1158 2537
BsrFI r'CCGG_y
Cuts at: 1693 1874 2083 1693
Size: 181 209 3305
Fragments arranged by size:
3305 209 181
BssSI C'ACGA_G
Cuts at: 1966 2324 1966
Size: 358 3337
BstAPI GCAn_nnn'nTGC
Cuts at: 1072 1072
Size: 3695
BstBI TT'CG_AA
Cuts at: 2056 2056
Size: 3695
BstYI r'GATC_y
Cuts at: 1 720 1187 1210 1545 1791 2803 2889 2901
Size: 719 467 23 335 246 1012 86 12
Cuts at: 2901 3247 1
Size: 346 449
Fragments arranged by size:
1012 719 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3695
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1645 1826 1989 1645
Size: 181 163 3351
Fragments arranged by size:
3351 181 163
BtsI GCAGTG_nn'
Cuts at: 938 938
Size: 3695
ClaI AT'CG_AT
Cuts at: 476 476
Size: 3695
DraI TTT'AAA
Cuts at: 694 694
Size: 3695
DrdI GACnn_nn'nnGTC
Cuts at: 1401 2259 1401
Size: 858 2837
EaeI y'GGCC_r
Cuts at: 1280 1454 1845 1872 3217 1280
Size: 174 391 27 1345 1758
Fragments arranged by size:
1758 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1280 1280
Size: 3695
EarI CTCTTCn'nnn_
Cuts at: 371 1718 1928 371
Size: 1347 210 2138
Fragments arranged by size:
2138 1347 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1903 2213 2359 3505 1903
Size: 310 146 1146 2093
Fragments arranged by size:
2093 1146 310 146
EcoICRI GAG'CTC
Cuts at: 123 707 123
Size: 584 3111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 1520 1952 2699 2967 650
Size: 870 432 747 268 1378
Fragments arranged by size:
1378 870 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 834 834
Size: 3695
EcoRI G'AATT_C
Cuts at: 602 699 602
Size: 97 3598
EcoRV GAT'ATC
Cuts at: 366 366
Size: 3695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 680 1714 680
Size: 1034 2661
FspI TGC'GCA
Cuts at: 1476 1476
Size: 3695
HaeII r_GCGC'y
Cuts at: 115 1129 1377 2399 3378 115
Size: 1014 248 1022 979 432
Fragments arranged by size:
1022 1014 979 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1537 1567 1569 1599 1537
Size: 30 2 30 3633
Fragments arranged by size:
3633 30 30 2
HincII GTy'rAC
Cuts at: 734 734
Size: 3695
KasI G'GCGC_C
Cuts at: 1125 1373 1125
Size: 248 3447
KpnI G_GTAC'C
Cuts at: 715 715
Size: 3695
MluI A'CGCG_T
Cuts at: 3128 3128
Size: 3695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2366 2550 2366
Size: 184 3511
MscI TGG'CCA
Cuts at: 1456 1456
Size: 3695
MslI CAynn'nnrTG
Cuts at: 1684 1811 1684
Size: 127 3568
MspA1I CmG'CkG
Cuts at: 292 806 1120 1480 2493 2738 292
Size: 514 314 360 1013 245 1249
Fragments arranged by size:
1249 1013 514 360 314 245
NaeI GCC'GGC
Cuts at: 1876 1876
Size: 3695
NarI GG'CG_CC
Cuts at: 1126 1374 1126
Size: 248 3447
NcoI C'CATG_G
Cuts at: 107 107
Size: 3695
NgoMIV G'CCGG_C
Cuts at: 1874 1874
Size: 3695
NspI r_CATG'y
Cuts at: 748 1248 2155 748
Size: 500 907 2288
Fragments arranged by size:
2288 907 500
PciI A'CATG_T
Cuts at: 2151 2151
Size: 3695
PmeI GTTT'AAAC
Cuts at: 694 694
Size: 3695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2834 2866 2834
Size: 32 3663
PstI C_TGCA'G
Cuts at: 348 742 1427 348
Size: 394 685 2616
Fragments arranged by size:
2616 685 394
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3695
PvuII CAG'CTG
Cuts at: 292 1120 1480 292
Size: 828 360 2507
Fragments arranged by size:
2507 828 360
RsrII CG'GwC_CG
Cuts at: 1890 1890
Size: 3695
SacI G_AGCT'C
Cuts at: 125 709 125
Size: 584 3111
SalI G'TCGA_C
Cuts at: 732 732
Size: 3695
SapI GCTCTTCn'nnn_
Cuts at: 1718 1928 1718
Size: 210 3485
SbfI CC_TGCA'GG
Cuts at: 742 742
Size: 3695
ScaI AGT'ACT
Cuts at: 3212 3212
Size: 3695
SfcI C'TryA_G
Cuts at: 33 344 738 1423 2416 2607 33
Size: 311 394 685 993 191 1121
Fragments arranged by size:
1121 993 685 394 311 191
SfoI GGC'GCC
Cuts at: 1127 1375 1127
Size: 248 3447
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
SmaI CCC'GGG
Cuts at: 717 717
Size: 3695
SmlI C'TyrA_G
Cuts at: 850 1765 2257 2519 850
Size: 915 492 262 2026
Fragments arranged by size:
2026 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3695
SphI G_CATG'C
Cuts at: 748 1248 748
Size: 500 3195
SspI AAT'ATT
Cuts at: 438 438
Size: 3695
StyI C'CwwG_G
Cuts at: 107 829 1183 107
Size: 722 354 2619
Fragments arranged by size:
2619 722 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 393 1278 1411 1870 2079 393
Size: 885 133 459 209 2009
Fragments arranged by size:
2009 885 459 209 133
TatI w'GTAC_w
Cuts at: 326 3210 326
Size: 2884 811
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 3663
Tth111I GACn'n_nGTC
Cuts at: 1492 1492
Size: 3695
XbaI T'CTAG_A
Cuts at: 726 726
Size: 3695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1190 1190
Size: 3695
XmaI C'CCGG_G
Cuts at: 715 715
Size: 3695
XmnI GAAnn'nnTTC
Cuts at: 305 305
Size: 3695
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BcgI BfrBI BglI BmgBI BmtI
BpmI BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoNI
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI