Restriction map for
pF1KB4908
(Circular) (Six-base) MAPSORT of: pF1KB4908.seq Check: 3473 from: 1 to: 5045
pF1KB4908 5045 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 556 618 1166 1309 1889 556
Size: 62 548 143 580 3712
Fragments arranged by size:
3712 580 548 143 62
AatII G_ACGT'C
Cuts at: 629 629
Size: 5045
AccI GT'mk_AC
Cuts at: 2083 2083
Size: 5045
Acc65I G'GTAC_C
Cuts at: 2061 2061
Size: 5045
AclI AA'CG_TT
Cuts at: 2039 4885 2039
Size: 2846 2199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 1029 1113 1449 1533 1617 1701 2870 3302
Size: 548 84 336 84 84 84 1169 432
Cuts at: 3302 4049 4317 481
Size: 747 268 1209
Fragments arranged by size:
1209 1169 747 548 432 336 268 84
84 84 84
AflIII A'CryG_T
Cuts at: 675 1475 2397 3501 4478 675
Size: 800 922 1104 977 1242
Fragments arranged by size:
1242 1104 977 922 800
AgeI A'CCGG_T
Cuts at: 1187 1675 3433 1187
Size: 488 1758 2799
Fragments arranged by size:
2799 1758 488
AleI CACnn'nnGTG
Cuts at: 1676 1676
Size: 5045
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1159 1174 1159
Size: 15 5030
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 832 864 1336 1368 832
Size: 32 472 32 4509
Fragments arranged by size:
4509 472 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1115 1367 1836 2350 3917 1115
Size: 252 469 514 1567 2243
Fragments arranged by size:
2243 1567 514 469 252
ApaI G_GGCC'C
Cuts at: 443 443
Size: 5045
ApaLI G'TGCA_C
Cuts at: 1069 1165 1489 1573 1657 3815 1069
Size: 96 324 84 84 2158 2299
Fragments arranged by size:
2299 2158 324 96 84 84
ApoI r'AATT_y
Cuts at: 640 2010 2049 4398 4654 640
Size: 1370 39 2349 256 1031
Fragments arranged by size:
2349 1370 1031 256 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 5045
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5045
AvaI C'yCGr_G
Cuts at: 130 184 682 2065 130
Size: 54 498 1383 3110
Fragments arranged by size:
3110 1383 498 54
AvrII C'CTAG_G
Cuts at: 351 351
Size: 5045
BamHI G'GATC_C
Cuts at: 156 1947 2070 156
Size: 1791 123 3131
Fragments arranged by size:
3131 1791 123
BanI G'GyrC_C
Cuts at: 468 557 600 832 2061 2475 2723 2758 468
Size: 89 43 232 1229 414 248 35 2755
Fragments arranged by size:
2755 1229 414 248 232 89 43 35
BanII G_rGCy'C
Cuts at: 131 443 518 889 1813 2059 3089 131
Size: 312 75 371 924 246 1030 2087
Fragments arranged by size:
2087 1030 924 371 312 246 75
BbeI G_GCGC'C
Cuts at: 561 604 836 2479 2727 561
Size: 43 232 1643 248 2879
Fragments arranged by size:
2879 1643 248 232 43
BbsI GAAGACnn'nnnn_
Cuts at: 142 199 142
Size: 57 4988
BciVI GTATCCnnnnn_n'
Cuts at: 2943 3704 5045 2943
Size: 761 1341 2943
Fragments arranged by size:
2943 1341 761
BclI T'GATC_A
Cuts at: 2565 2565
Size: 5045
BglII A'GATC_T
Cuts at: 2560 4597 2560
Size: 2037 3008
BlpI GC'TnA_GC
Cuts at: 1147 1885 2157 1147
Size: 738 272 4035
Fragments arranged by size:
4035 738 272
Bme1580I G_kGCm'C
Cuts at: 443 473 1073 1169 1493 1577 1661 2670 2763
Size: 30 600 96 324 84 84 1009 93
Cuts at: 2763 3819 443
Size: 1056 1669
Fragments arranged by size:
1669 1056 1009 600 324 96 93 84
84 30
BmgBI CAC'GTC
Cuts at: 1944 1944
Size: 5045
BmrI ACTGGGnnnn_n'
Cuts at: 2257 2338 2673 4817 2257
Size: 81 335 2144 2485
Fragments arranged by size:
2485 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2048 3412 2048
Size: 1364 3681
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 987 1003 1827 987
Size: 16 824 4205
Fragments arranged by size:
4205 824 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2221 3100 3592 3890 2221
Size: 879 492 298 3376
Fragments arranged by size:
3376 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4749 4749
Size: 5045
BsaAI yAC'GTr
Cuts at: 1899 2398 1899
Size: 499 4546
BsaBI GATnn'nnATC
Cuts at: 6 2559 4596 6
Size: 2553 2037 455
Fragments arranged by size:
2553 2037 455
BsaHI Gr'CG_yC
Cuts at: 558 601 626 833 2476 2724 3426 4922 558
Size: 43 25 207 1643 248 702 1496 681
Fragments arranged by size:
1643 1496 702 681 248 207 43 25
BsaWI w'CCGG_w
Cuts at: 1187 1675 2234 2755 3433 3707 3854 4265 1187
Size: 488 559 521 678 274 147 411 1967
Fragments arranged by size:
1967 678 559 521 488 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 832 862 1756 1786 832
Size: 30 894 30 4091
Fragments arranged by size:
4091 894 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 286 418 594 879 286
Size: 132 176 285 4452
Fragments arranged by size:
4452 285 176 132
BseYI C'CCAG_C
Cuts at: 668 1914 2470 3228 3805 4301 668
Size: 1246 556 758 577 496 1412
Fragments arranged by size:
1412 1246 758 577 556 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 782 886 1128 1380 1716 1822 1940 349
Size: 433 104 242 252 336 106 118 3454
Fragments arranged by size:
3454 433 336 252 242 118 106 104
BsiEI CG_ry'CG
Cuts at: 104 2587 2633 3841 104
Size: 2483 46 1208 1308
Fragments arranged by size:
2483 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 573 889 973 989 1073 1169 1493 1577 1661
Size: 316 84 16 84 96 324 84 84
Cuts at: 1661 1813 1829 2059 2837 3027 3819 573
Size: 152 16 230 778 190 792 1799
Fragments arranged by size:
1799 792 778 324 316 230 190 152
96 84 84 84 84 16 16
BsmI GAATG_Cn'
Cuts at: 952 952
Size: 5045
Bsp1286I G_dGCh'C
Cuts at: 131 443 473 518 573 889 973 989 1073
Size: 312 30 45 55 316 84 16 84
Cuts at: 1073 1169 1493 1577 1661 1813 1829 2059 2670
Size: 96 324 84 84 152 16 230 611
Cuts at: 2670 2763 2837 3027 3089 3819 131
Size: 93 74 190 62 730 1357
Fragments arranged by size:
1357 730 611 324 316 312 230 190
152 96 93 84 84 84 84 74
62 55 45 30 16 16
BspEI T'CCGG_A
Cuts at: 4265 4265
Size: 5045
BspHI T'CATG_A
Cuts at: 4221 4221
Size: 5045
BspMI ACCTGCnnnn'nnnn_
Cuts at: 556 618 1166 1309 1490 1889 2095 2309 2611
Size: 62 548 143 181 399 206 214 302
Cuts at: 2611 2992 556
Size: 381 2609
Fragments arranged by size:
2609 548 399 381 302 214 206 181
143 62
BsrBI CCG'CTC
Cuts at: 50 528 1095 1391 3337 3391 4875 5040 50
Size: 478 567 296 1946 54 1484 165 55
Fragments arranged by size:
1946 1484 567 478 296 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2957 2957
Size: 5045
BsrFI r'CCGG_y
Cuts at: 504 815 905 989 1003 1171 1187 1493 1577
Size: 311 90 84 14 168 16 306 84
Cuts at: 1577 1675 3043 3224 3433 504
Size: 98 1368 181 209 2116
Fragments arranged by size:
2116 1368 311 306 209 181 168 98
90 84 84 16 14
BssSI C'ACGA_G
Cuts at: 1486 3316 3674 1486
Size: 1830 358 2857
Fragments arranged by size:
2857 1830 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1115 1367 1535 1703 1927 2422 1115
Size: 252 168 168 224 495 3738
Fragments arranged by size:
3738 495 252 224 168 168
BstBI TT'CG_AA
Cuts at: 3406 3406
Size: 5045
BstXI CCAn_nnnn'nTGG
Cuts at: 981 981
Size: 5045
BstYI r'GATC_y
Cuts at: 1 113 156 1331 1947 2070 2537 2560 2895
Size: 112 43 1175 616 123 467 23 335
Cuts at: 2895 3141 4153 4239 4251 4597 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1175 1012 616 467 449 346 335 246
123 112 86 43 23 12
Bsu36I CC'TnA_GG
Cuts at: 649 649
Size: 5045
BtgI C'CryG_G
Cuts at: 107 409 614 107
Size: 302 205 4538
Fragments arranged by size:
4538 302 205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 956 2995 3176 3339 956
Size: 2039 181 163 2662
Fragments arranged by size:
2662 2039 181 163
BtsI GCAGTG_nn'
Cuts at: 361 2288 361
Size: 1927 3118
DraI TTT'AAA
Cuts at: 2044 2044
Size: 5045
DrdI GACnn_nn'nnGTC
Cuts at: 2751 3609 2751
Size: 858 4187
EaeI y'GGCC_r
Cuts at: 1859 2630 2804 3195 3222 4567 1859
Size: 771 174 391 27 1345 2337
Fragments arranged by size:
2337 1345 771 391 174 27
EagI C'GGCC_G
Cuts at: 2630 2630
Size: 5045
EarI CTCTTCn'nnn_
Cuts at: 667 3068 3278 667
Size: 2401 210 2434
Fragments arranged by size:
2434 2401 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 868 3253 3563 3709 4855 868
Size: 2385 310 146 1146 1058
Fragments arranged by size:
2385 1146 1058 310 146
EcoICRI GAG'CTC
Cuts at: 887 1811 2057 887
Size: 924 246 3875
Fragments arranged by size:
3875 924 246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 987 1003 1029 1113 1449 1533 1617 1701
Size: 506 16 26 84 336 84 84 84
Cuts at: 1701 1827 2870 3302 4049 4317 481
Size: 126 1043 432 747 268 1209
Fragments arranged by size:
1209 1043 747 506 432 336 268 126
84 84 84 84 26 16
EcoO109I rG'GnC_Cy
Cuts at: 302 355 439 440 691 744 2184 302
Size: 53 84 1 251 53 1440 3163
Fragments arranged by size:
3163 1440 251 84 53 53 1
EcoRI G'AATT_C
Cuts at: 640 2049 640
Size: 1409 3636
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1035 3064 1035
Size: 2029 3016
FspI TGC'GCA
Cuts at: 2826 2826
Size: 5045
HaeII r_GCGC'y
Cuts at: 561 604 836 912 1115 2479 2727 3749 4728
Size: 43 232 76 203 1364 248 1022 979
Cuts at: 4728 561
Size: 878
Fragments arranged by size:
1364 1022 979 878 248 232 203 76
43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 367 372 399 404 2887 2917 2919
Size: 32 157 5 27 5 2483 30 2
Cuts at: 2919 2949 178
Size: 30 2274
Fragments arranged by size:
2483 2274 157 32 30 30 27 5
5 2
HincII GTy'rAC
Cuts at: 283 2084 283
Size: 1801 3244
KasI G'GCGC_C
Cuts at: 557 600 832 2475 2723 557
Size: 43 232 1643 248 2879
Fragments arranged by size:
2879 1643 248 232 43
KpnI G_GTAC'C
Cuts at: 2065 2065
Size: 5045
MluI A'CGCG_T
Cuts at: 675 1475 4478 675
Size: 800 3003 1242
Fragments arranged by size:
3003 1242 800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 305 1603 3716 3900 305
Size: 1298 2113 184 1450
Fragments arranged by size:
2113 1450 1298 184
MscI TGG'CCA
Cuts at: 1861 2806 1861
Size: 945 4100
MslI CAynn'nnrTG
Cuts at: 1676 3034 3161 1676
Size: 1358 127 3560
Fragments arranged by size:
3560 1358 127
MspA1I CmG'CkG
Cuts at: 1049 1133 1340 1469 1508 1637 2156 2470 2830
Size: 84 207 129 39 129 519 314 360
Cuts at: 2830 3843 4088 1049
Size: 1013 245 2006
Fragments arranged by size:
2006 1013 519 360 314 245 207 129
129 84 39
NaeI GCC'GGC
Cuts at: 817 3226 817
Size: 2409 2636
NarI GG'CG_CC
Cuts at: 558 601 833 2476 2724 558
Size: 43 232 1643 248 2879
Fragments arranged by size:
2879 1643 248 232 43
NcoI C'CATG_G
Cuts at: 107 409 614 107
Size: 302 205 4538
Fragments arranged by size:
4538 302 205
NgoMIV G'CCGG_C
Cuts at: 815 3224 815
Size: 2409 2636
NspI r_CATG'y
Cuts at: 420 1155 2098 2598 3505 420
Size: 735 943 500 907 1960
Fragments arranged by size:
1960 943 907 735 500
PasI CC'CwG_GG
Cuts at: 596 2019 596
Size: 1423 3622
PciI A'CATG_T
Cuts at: 3501 3501
Size: 5045
PflMI CCAn_nnn'nTGG
Cuts at: 620 620
Size: 5045
PmeI GTTT'AAAC
Cuts at: 2044 2044
Size: 5045
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 832 864 4184 4216 832
Size: 32 3320 32 1661
Fragments arranged by size:
3320 1661 32 32
PpuMI rG'GwC_Cy
Cuts at: 355 691 355
Size: 336 4709
PspOMI G'GGCC_C
Cuts at: 439 439
Size: 5045
PstI C_TGCA'G
Cuts at: 578 1138 1615 2092 2777 578
Size: 560 477 477 685 2846
Fragments arranged by size:
2846 685 560 477 477
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5045
PvuII CAG'CTG
Cuts at: 1049 1133 1469 1637 2470 2830 1049
Size: 84 336 168 833 360 3264
Fragments arranged by size:
3264 833 360 336 168 84
RsrII CG'GwC_CG
Cuts at: 3240 3240
Size: 5045
SacI G_AGCT'C
Cuts at: 889 1813 2059 889
Size: 924 246 3875
Fragments arranged by size:
3875 924 246
SalI G'TCGA_C
Cuts at: 2082 2082
Size: 5045
SanDI GG'GwC_CC
Cuts at: 691 691
Size: 5045
SapI GCTCTTCn'nnn_
Cuts at: 3068 3278 3068
Size: 210 4835
SbfI CC_TGCA'GG
Cuts at: 2092 2092
Size: 5045
ScaI AGT'ACT
Cuts at: 4562 4562
Size: 5045
SfcI C'TryA_G
Cuts at: 33 574 632 1134 1611 2088 2773 3766 3957
Size: 541 58 502 477 477 685 993 191
Cuts at: 3957 33
Size: 1121
Fragments arranged by size:
1121 993 685 541 502 477 477 191
58
SfoI GGC'GCC
Cuts at: 559 602 834 2477 2725 559
Size: 43 232 1643 248 2879
Fragments arranged by size:
2879 1643 248 232 43
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5045
SgrAI Cr'CCGG_yG
Cuts at: 905 989 1003 1171 1493 1577 1675 905
Size: 84 14 168 322 84 98 4275
Fragments arranged by size:
4275 322 168 98 84 84 14
SmaI CCC'GGG
Cuts at: 2067 2067
Size: 5045
SmlI C'TyrA_G
Cuts at: 184 2200 3115 3607 3869 184
Size: 2016 915 492 262 1360
Fragments arranged by size:
2016 1360 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5045
SphI G_CATG'C
Cuts at: 420 1155 2098 2598 420
Size: 735 943 500 2867
Fragments arranged by size:
2867 943 735 500
StuI AGG'CCT
Cuts at: 958 1042 1126 1210 1294 1378 1630 958
Size: 84 84 84 84 84 252 4373
Fragments arranged by size:
4373 252 84 84 84 84 84
StyI C'CwwG_G
Cuts at: 107 224 351 409 614 740 1856 2179 2533
Size: 117 127 58 205 126 1116 323 354
Cuts at: 2533 107
Size: 2619
Fragments arranged by size:
2619 1116 354 323 205 127 126 117
58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 280 1906 2628 2761 3220 3429 280
Size: 1626 722 133 459 209 1896
Fragments arranged by size:
1896 1626 722 459 209 133
TatI w'GTAC_w
Cuts at: 4560 4560
Size: 5045
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4562 4594 4562
Size: 32 5013
Tth111I GACn'n_nGTC
Cuts at: 2842 2842
Size: 5045
XbaI T'CTAG_A
Cuts at: 2076 2076
Size: 5045
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1959 2540 1959
Size: 581 4464
XhoI C'TCGA_G
Cuts at: 184 184
Size: 5045
XmaI C'CCGG_G
Cuts at: 2065 2065
Size: 5045
XmnI GAAnn'nnTTC
Cuts at: 874 1015 1042 1126 1378 1435 1462 1519 1603
Size: 141 27 84 252 57 27 57 84
Cuts at: 1603 1630 874
Size: 27 4289
Fragments arranged by size:
4289 252 141 84 84 57 57 27
27 27
ZraI GAC'GTC
Cuts at: 627 627
Size: 5045
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AscI BaeI BbvCI BcgI BfrBI
BglI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SnaBI SrfI SspI SwaI