Restriction map for
pF1KB4902
(Circular) (Six-base) MAPSORT of: pF1KB4902.seq Check: 4033 from: 1 to: 5345
pF1KB4902 5345 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1379 2383 1379
Size: 1004 4341
Acc65I G'GTAC_C
Cuts at: 2361 2361
Size: 5345
AclI AA'CG_TT
Cuts at: 5185 5185
Size: 5345
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 607 1055 2047 3170 3602 3955 4617 274
Size: 333 448 992 1123 432 353 662 1002
Fragments arranged by size:
1123 1002 992 662 448 432 353 333
AflII C'TTAA_G
Cuts at: 2304 2304
Size: 5345
AflIII A'CryG_T
Cuts at: 861 2697 4497 4778 861
Size: 1836 1800 281 1428
Fragments arranged by size:
1836 1800 1428 281
AlwNI CAG_nnn'CTG
Cuts at: 1170 2650 4088 1170
Size: 1480 1438 2427
Fragments arranged by size:
2427 1480 1438
ApaLI G'TGCA_C
Cuts at: 4183 4183
Size: 5345
ApoI r'AATT_y
Cuts at: 466 675 784 1410 2290 2349 4698 4954 466
Size: 209 109 626 880 59 2349 256 857
Fragments arranged by size:
2349 880 857 626 256 209 109 59
AseI AT'TA_AT
Cuts at: 20 20
Size: 5345
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5345
AvaI C'yCGr_G
Cuts at: 818 1652 2365 818
Size: 834 713 3798
Fragments arranged by size:
3798 834 713
BamHI G'GATC_C
Cuts at: 573 2268 2370 573
Size: 1695 102 3548
Fragments arranged by size:
3548 1695 102
BanI G'GyrC_C
Cuts at: 2004 2361 2775 3023 3058 2004
Size: 357 414 248 35 4291
Fragments arranged by size:
4291 414 357 248 35
BanII G_rGCy'C
Cuts at: 953 2359 3389 953
Size: 1406 1030 2909
Fragments arranged by size:
2909 1406 1030
BbeI G_GCGC'C
Cuts at: 2779 3027 2779
Size: 248 5097
BbsI GAAGACnn'nnnn_
Cuts at: 262 1275 1759 262
Size: 1013 484 3848
Fragments arranged by size:
3848 1013 484
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 659 693 659
Size: 34 5311
BciVI GTATCCnnnnn_n'
Cuts at: 3243 4299 5345 3243
Size: 1056 1046 3243
Fragments arranged by size:
3243 1056 1046
BclI T'GATC_A
Cuts at: 2865 2865
Size: 5345
BglII A'GATC_T
Cuts at: 1236 1805 1868 2860 4897 1236
Size: 569 63 992 2037 1684
Fragments arranged by size:
2037 1684 992 569 63
BlpI GC'TnA_GC
Cuts at: 2457 2457
Size: 5345
Bme1580I G_kGCm'C
Cuts at: 2111 2970 3063 4187 2111
Size: 859 93 1124 3269
Fragments arranged by size:
3269 1124 859 93
BmrI ACTGGGnnnn_n'
Cuts at: 1446 1639 2557 2638 2973 5117 1446
Size: 193 918 81 335 2144 1674
Fragments arranged by size:
2144 1674 918 335 193 81
BmtI G_CTAG'C
Cuts at: 2324 2324
Size: 5345
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 242 3712 242
Size: 3470 1875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 815 815
Size: 5345
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2521 3400 4114 4412 2521
Size: 879 714 298 3454
Fragments arranged by size:
3454 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1396 5049 1396
Size: 3653 1692
BsaAI yAC'GTr
Cuts at: 2106 2698 2106
Size: 592 4753
BsaBI GATnn'nnATC
Cuts at: 6 2224 2859 4896 6
Size: 2218 635 2037 455
Fragments arranged by size:
2218 2037 635 455
BsaHI Gr'CG_yC
Cuts at: 2776 3024 3726 5222 2776
Size: 248 702 1496 2899
Fragments arranged by size:
2899 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2534 3055 3733 4144 4291 4565 2534
Size: 521 678 411 147 274 3314
Fragments arranged by size:
3314 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1041 1071 1041
Size: 30 5315
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1054 1553 1730 2000 1054
Size: 499 177 270 4399
Fragments arranged by size:
4399 499 270 177
BseYI C'CCAG_C
Cuts at: 316 1892 2770 3528 4193 4601 316
Size: 1576 878 758 665 408 1060
Fragments arranged by size:
1576 1060 878 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 1702 191
Size: 1511 3834
BsiEI CG_ry'CG
Cuts at: 104 2887 2933 4163 104
Size: 2783 46 1230 1286
Fragments arranged by size:
2783 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2359 3137 3327 4187 2359
Size: 778 190 860 3517
Fragments arranged by size:
3517 860 778 190
BsmI GAATG_Cn'
Cuts at: 289 945 289
Size: 656 4689
Bsp1286I G_dGCh'C
Cuts at: 953 2111 2359 2970 3063 3137 3327 3389 4187
Size: 1158 248 611 93 74 190 62 798
Cuts at: 4187 953
Size: 2111
Fragments arranged by size:
2111 1158 798 611 248 190 93 74
62
BspHI T'CATG_A
Cuts at: 1146 3777 1146
Size: 2631 2714
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2395 2609 2911 3292 2395
Size: 214 302 381 4448
Fragments arranged by size:
4448 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3637 3691 5175 5340 50
Size: 3587 54 1484 165 55
Fragments arranged by size:
3587 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 284 2114 3257 284
Size: 1830 1143 2372
Fragments arranged by size:
2372 1830 1143
BsrFI r'CCGG_y
Cuts at: 3343 3524 3343
Size: 181 5164
BssSI C'ACGA_G
Cuts at: 3616 4324 3616
Size: 708 4637
BstAPI GCAn_nnn'nTGC
Cuts at: 1774 2722 1774
Size: 948 4397
BstBI TT'CG_AA
Cuts at: 3706 3706
Size: 5345
BstEII G'GTnAC_C
Cuts at: 1639 1639
Size: 5345
BstYI r'GATC_y
Cuts at: 1 573 1236 1805 1868 2268 2370 2837 2860
Size: 572 663 569 63 400 102 467 23
Cuts at: 2860 3195 3441 3747 3759 3845 4897 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 663 572 569 467 449 400 335
306 246 102 86 63 23 12
Bsu36I CC'TnA_GG
Cuts at: 2012 2012
Size: 5345
BtgI C'CryG_G
Cuts at: 107 569 1397 107
Size: 462 828 4055
Fragments arranged by size:
4055 828 462
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3295 3476 3639 3295
Size: 181 163 5001
Fragments arranged by size:
5001 181 163
BtsI GCAGTG_nn'
Cuts at: 1457 2588 1457
Size: 1131 4214
DraI TTT'AAA
Cuts at: 2344 2344
Size: 5345
DrdI GACnn_nn'nnGTC
Cuts at: 1156 3051 4395 1156
Size: 1895 1344 2106
Fragments arranged by size:
2106 1895 1344
EaeI y'GGCC_r
Cuts at: 201 1394 2930 3104 3495 3522 4867 201
Size: 1193 1536 174 391 27 1345 679
Fragments arranged by size:
1536 1345 1193 679 391 174 27
EagI C'GGCC_G
Cuts at: 2930 2930
Size: 5345
EarI CTCTTCn'nnn_
Cuts at: 1031 1259 1988 3368 3578 1031
Size: 228 729 1380 210 2798
Fragments arranged by size:
2798 1380 729 228 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3553 4295 4441 5155 3553
Size: 742 146 714 3743
Fragments arranged by size:
3743 742 714 146
EcoICRI GAG'CTC
Cuts at: 2357 2357
Size: 5345
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 607 815 1055 2047 3170 3602 3955 4617
Size: 333 208 240 992 1123 432 353 662
Cuts at: 4617 274
Size: 1002
Fragments arranged by size:
1123 1002 992 662 432 353 333 240
208
EcoNI CCTnn'n_nnAGG
Cuts at: 602 602
Size: 5345
EcoO109I rG'GnC_Cy
Cuts at: 221 1735 2484 221
Size: 1514 749 3082
Fragments arranged by size:
3082 1514 749
EcoRI G'AATT_C
Cuts at: 2349 2349
Size: 5345
EcoRV GAT'ATC
Cuts at: 1569 1569
Size: 5345
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 927 1887 2077 3364 927
Size: 960 190 1287 2908
Fragments arranged by size:
2908 1287 960 190
FspI TGC'GCA
Cuts at: 3126 3126
Size: 5345
HaeII r_GCGC'y
Cuts at: 2779 3027 4257 5028 2779
Size: 248 1230 771 3096
Fragments arranged by size:
3096 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 139 171 181 213 1341 1373 1797 1829 3187
Size: 32 10 32 1128 32 424 32 1358
Cuts at: 3187 3217 3219 3249 139
Size: 30 2 30 2235
Fragments arranged by size:
2235 1358 1128 424 32 32 32 32
30 30 10 2
HincII GTy'rAC
Cuts at: 1161 2384 1161
Size: 1223 4122
HindIII A'AGCT_T
Cuts at: 535 2023 2141 535
Size: 1488 118 3739
Fragments arranged by size:
3739 1488 118
KasI G'GCGC_C
Cuts at: 2775 3023 2775
Size: 248 5097
KpnI G_GTAC'C
Cuts at: 2365 2365
Size: 5345
MfeI C'AATT_G
Cuts at: 165 2186 165
Size: 2021 3324
MluI A'CGCG_T
Cuts at: 4778 4778
Size: 5345
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 458 621 825 1811 2117 4104 4288 458
Size: 163 204 986 306 1987 184 1515
Fragments arranged by size:
1987 1515 986 306 204 184 163
MscI TGG'CCA
Cuts at: 203 3106 203
Size: 2903 2442
MslI CAynn'nnrTG
Cuts at: 1219 1462 2104 3334 3461 1219
Size: 243 642 1230 127 3103
Fragments arranged by size:
3103 1230 642 243 127
MspA1I CmG'CkG
Cuts at: 2133 2456 2770 3130 3914 4159 2133
Size: 323 314 360 784 245 3319
Fragments arranged by size:
3319 784 360 323 314 245
NaeI GCC'GGC
Cuts at: 3526 3526
Size: 5345
NarI GG'CG_CC
Cuts at: 2776 3024 2776
Size: 248 5097
NcoI C'CATG_G
Cuts at: 107 569 107
Size: 462 4883
NgoMIV G'CCGG_C
Cuts at: 3524 3524
Size: 5345
NheI G'CTAG_C
Cuts at: 2320 2320
Size: 5345
NspI r_CATG'y
Cuts at: 126 211 865 2398 2898 4501 126
Size: 85 654 1533 500 1603 970
Fragments arranged by size:
1603 1533 970 654 500 85
PciI A'CATG_T
Cuts at: 861 4497 861
Size: 3636 1709
PflMI CCAn_nnn'nTGG
Cuts at: 898 1582 898
Size: 684 4661
PmeI GTTT'AAAC
Cuts at: 2344 2344
Size: 5345
PmlI CAC'GTG
Cuts at: 2106 2106
Size: 5345
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 3791
Size: 32 5313
PpuMI rG'GwC_Cy
Cuts at: 221 221
Size: 5345
PspXI vC'TCGA_Gb
Cuts at: 1652 1652
Size: 5345
PstI C_TGCA'G
Cuts at: 416 2392 3077 416
Size: 1976 685 2684
Fragments arranged by size:
2684 1976 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5345
PvuII CAG'CTG
Cuts at: 2133 2770 3130 2133
Size: 637 360 4348
Fragments arranged by size:
4348 637 360
RsrII CG'GwC_CG
Cuts at: 3540 3540
Size: 5345
SacI G_AGCT'C
Cuts at: 2359 2359
Size: 5345
SalI G'TCGA_C
Cuts at: 2382 2382
Size: 5345
SapI GCTCTTCn'nnn_
Cuts at: 1259 1988 3368 3578 1259
Size: 729 1380 210 3026
Fragments arranged by size:
3026 1380 729 210
SbfI CC_TGCA'GG
Cuts at: 2392 2392
Size: 5345
ScaI AGT'ACT
Cuts at: 1488 4862 1488
Size: 3374 1971
SfcI C'TryA_G
Cuts at: 33 143 412 1318 2260 2388 3073 4041 4232
Size: 110 269 906 942 128 685 968 191
Cuts at: 4232 33
Size: 1146
Fragments arranged by size:
1146 968 942 906 685 269 191 128
110
SfoI GGC'GCC
Cuts at: 2777 3025 2777
Size: 248 5097
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5345
SmaI CCC'GGG
Cuts at: 2367 2367
Size: 5345
SmlI C'TyrA_G
Cuts at: 1652 2304 2500 3415 4129 4391 1652
Size: 652 196 915 714 262 2606
Fragments arranged by size:
2606 915 714 652 262 196
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5345
SphI G_CATG'C
Cuts at: 126 2398 2898 126
Size: 2272 500 2573
Fragments arranged by size:
2573 2272 500
StuI AGG'CCT
Cuts at: 493 493
Size: 5345
StyI C'CwwG_G
Cuts at: 107 569 710 2479 2833 107
Size: 462 141 1769 354 2619
Fragments arranged by size:
2619 1769 462 354 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2928 3061 3520 3729 2928
Size: 133 459 209 4544
Fragments arranged by size:
4544 459 209 133
TatI w'GTAC_w
Cuts at: 1486 4860 1486
Size: 3374 1971
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1041 1073 4862 4894 1041
Size: 32 3789 32 1492
Fragments arranged by size:
3789 1492 32 32
Tth111I GACn'n_nGTC
Cuts at: 3142 3142
Size: 5345
XbaI T'CTAG_A
Cuts at: 774 1659 2376 774
Size: 885 717 3743
Fragments arranged by size:
3743 885 717
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1491 2840 1491
Size: 1349 3996
XhoI C'TCGA_G
Cuts at: 1652 1652
Size: 5345
XmaI C'CCGG_G
Cuts at: 2365 2365
Size: 5345
XmnI GAAnn'nnTTC
Cuts at: 470 470
Size: 5345
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PflMI PmeI PmlI PpiI PpuMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
Bpu10I BsiWI BsmBI BspEI BsrGI BssHII BstXI BstZ17I
ClaI CspCI DraIII FseI FspAI HpaI NdeI NotI
NruI NsiI PacI PasI PfoI PshAI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI