Restriction map for
pF1KB4894
(Circular) (Six-base) MAPSORT of: pF1KB4894.seq Check: 4070 from: 1 to: 4334
pF1KB4894 4334 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 1372 1372
Size: 4334
Acc65I G'GTAC_C
Cuts at: 1350 1350
Size: 4334
AclI AA'CG_TT
Cuts at: 4174 4174
Size: 4334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2159 2591 3338 3606 2159
Size: 432 747 268 2887
Fragments arranged by size:
2887 747 432 268
AflIII A'CryG_T
Cuts at: 339 742 936 1686 2790 3767 339
Size: 403 194 750 1104 977 906
Fragments arranged by size:
1104 977 906 750 403 194
AgeI A'CCGG_T
Cuts at: 2722 2722
Size: 4334
AleI CACnn'nnGTG
Cuts at: 482 482
Size: 4334
AlwNI CAG_nnn'CTG
Cuts at: 1639 3206 1639
Size: 1567 2767
ApaI G_GGCC'C
Cuts at: 856 856
Size: 4334
ApaLI G'TGCA_C
Cuts at: 277 3104 277
Size: 2827 1507
ApoI r'AATT_y
Cuts at: 1338 3687 3943 1338
Size: 2349 256 1729
Fragments arranged by size:
2349 1729 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4334
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
AvaI C'yCGr_G
Cuts at: 306 614 758 794 856 1354 306
Size: 308 144 36 62 498 3286
Fragments arranged by size:
3286 498 308 144 62 36
BamHI G'GATC_C
Cuts at: 893 1281 1359 893
Size: 388 78 3868
Fragments arranged by size:
3868 388 78
BanI G'GyrC_C
Cuts at: 241 375 413 597 799 1015 1350 1764 2012
Size: 134 38 184 202 216 335 414 248
Cuts at: 2012 2047 241
Size: 35 2528
Fragments arranged by size:
2528 414 335 248 216 202 184 134
38 35
BanII G_rGCy'C
Cuts at: 173 307 615 856 1180 1348 2378 173
Size: 134 308 241 324 168 1030 2129
Fragments arranged by size:
2129 1030 324 308 241 168 134
BbeI G_GCGC'C
Cuts at: 379 417 803 1768 2016 379
Size: 38 386 965 248 2697
Fragments arranged by size:
2697 965 386 248 38
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4300
BciVI GTATCCnnnnn_n'
Cuts at: 2232 2993 4334 2232
Size: 761 1341 2232
Fragments arranged by size:
2232 1341 761
BclI T'GATC_A
Cuts at: 1854 1854
Size: 4334
BglI GCCn_nnn'nGGC
Cuts at: 411 595 839 1110 1217 411
Size: 184 244 271 107 3528
Fragments arranged by size:
3528 271 244 184 107
BglII A'GATC_T
Cuts at: 1849 3886 1849
Size: 2037 2297
BlpI GC'TnA_GC
Cuts at: 1446 1446
Size: 4334
Bme1580I G_kGCm'C
Cuts at: 246 281 856 888 1959 2052 3108 246
Size: 35 575 32 1071 93 1056 1472
Fragments arranged by size:
1472 1071 1056 575 93 35 32
BmgBI CAC'GTC
Cuts at: 745 745
Size: 4334
BmrI ACTGGGnnnn_n'
Cuts at: 895 1546 1627 1962 4106 895
Size: 651 81 335 2144 1123
Fragments arranged by size:
2144 1123 651 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 4334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 1015 1035 1107 235
Size: 780 20 72 3462
Fragments arranged by size:
3462 780 72 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 937 1510 2389 2881 3179 587
Size: 350 573 879 492 298 1742
Fragments arranged by size:
1742 879 573 492 350 298
BsaI GGTCTCn'nnnn_
Cuts at: 385 4038 385
Size: 3653 681
BsaAI yAC'GTr
Cuts at: 276 939 1687 276
Size: 663 748 2923
Fragments arranged by size:
2923 748 663
BsaBI GATnn'nnATC
Cuts at: 6 1848 3885 6
Size: 1842 2037 455
Fragments arranged by size:
2037 1842 455
BsaHI Gr'CG_yC
Cuts at: 164 376 414 800 1765 2013 2715 4211 164
Size: 212 38 386 965 248 702 1496 287
Fragments arranged by size:
1496 965 702 386 287 248 212 38
BsaWI w'CCGG_w
Cuts at: 1523 2044 2722 2996 3143 3554 1523
Size: 521 678 274 147 411 2303
Fragments arranged by size:
2303 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 390 420 721 751 390
Size: 30 301 30 3973
Fragments arranged by size:
3973 301 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1112 1171 1291 1112
Size: 59 120 4155
Fragments arranged by size:
4155 120 59
BseYI C'CCAG_C
Cuts at: 528 1092 1759 2517 3094 3590 528
Size: 564 667 758 577 496 1272
Fragments arranged by size:
1272 758 667 577 564 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 496 793 904 160
Size: 336 297 111 3590
Fragments arranged by size:
3590 336 297 111
BsiEI CG_ry'CG
Cuts at: 104 158 681 1876 1922 3130 104
Size: 54 523 1195 46 1208 1308
Fragments arranged by size:
1308 1208 1195 523 54 46
BsiHKAI G_wGCw'C
Cuts at: 281 313 1348 2126 2316 3108 281
Size: 32 1035 778 190 792 1507
Fragments arranged by size:
1507 1035 792 778 190 32
Bsp1286I G_dGCh'C
Cuts at: 173 246 281 307 313 615 856 888 1180
Size: 73 35 26 6 302 241 32 292
Cuts at: 1180 1348 1959 2052 2126 2316 2378 3108 173
Size: 168 611 93 74 190 62 730 1399
Fragments arranged by size:
1399 730 611 302 292 241 190 168
93 74 73 62 35 32 26 6
BspEI T'CCGG_A
Cuts at: 3554 3554
Size: 4334
BspHI T'CATG_A
Cuts at: 3510 3510
Size: 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1384 1598 1900 2281 1384
Size: 214 302 381 3437
Fragments arranged by size:
3437 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1110 2626 2680 4164 4329 50
Size: 1060 1516 54 1484 165 55
Fragments arranged by size:
1516 1484 1060 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2246 2246
Size: 4334
BsrFI r'CCGG_y
Cuts at: 515 846 2332 2513 2722 515
Size: 331 1486 181 209 2127
Fragments arranged by size:
2127 1486 331 209 181
BssHII G'CGCG_C
Cuts at: 255 257 255
Size: 2 4332
BssSI C'ACGA_G
Cuts at: 2605 2963 2605
Size: 358 3976
BstAPI GCAn_nnn'nTGC
Cuts at: 1325 1711 1325
Size: 386 3948
BstBI TT'CG_AA
Cuts at: 2695 2695
Size: 4334
BstXI CCAn_nnnn'nTGG
Cuts at: 372 372
Size: 4334
BstYI r'GATC_y
Cuts at: 1 893 1281 1359 1826 1849 2184 2430 3442
Size: 892 388 78 467 23 335 246 1012
Cuts at: 3442 3528 3540 3886 1
Size: 86 12 346 449
Fragments arranged by size:
1012 892 467 449 388 346 335 246
86 78 23 12
Bsu36I CC'TnA_GG
Cuts at: 247 1115 247
Size: 868 3466
BtgI C'CryG_G
Cuts at: 158 194 648 767 158
Size: 36 454 119 3725
Fragments arranged by size:
3725 454 119 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2284 2465 2628 2284
Size: 181 163 3990
Fragments arranged by size:
3990 181 163
BtsI GCAGTG_nn'
Cuts at: 827 886 921 1577 827
Size: 59 35 656 3584
Fragments arranged by size:
3584 656 59 35
DraI TTT'AAA
Cuts at: 1333 1333
Size: 4334
DrdI GACnn_nn'nnGTC
Cuts at: 2040 2898 2040
Size: 858 3476
EaeI y'GGCC_r
Cuts at: 186 668 827 1919 2093 2484 2511 3856 186
Size: 482 159 1092 174 391 27 1345 664
Fragments arranged by size:
1345 1092 664 482 391 174 159 27
EagI C'GGCC_G
Cuts at: 1919 1919
Size: 4334
EarI CTCTTCn'nnn_
Cuts at: 2357 2567 2357
Size: 210 4124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2542 2852 2998 4144 2542
Size: 310 146 1146 2732
Fragments arranged by size:
2732 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1346 1346
Size: 4334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 1015 1035 1107 2159 2591 3338 3606 235
Size: 780 20 72 1052 432 747 268 963
Fragments arranged by size:
1052 963 780 747 432 268 72 20
EcoO109I rG'GnC_Cy
Cuts at: 525 853 907 1097 1197 1473 525
Size: 328 54 190 100 276 3386
Fragments arranged by size:
3386 328 276 190 100 54
EcoRI G'AATT_C
Cuts at: 1338 1338
Size: 4334
EcoRV GAT'ATC
Cuts at: 969 969
Size: 4334
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 4334
FspI TGC'GCA
Cuts at: 2115 2115
Size: 4334
HaeII r_GCGC'y
Cuts at: 230 379 417 803 871 1768 2016 3038 4017
Size: 149 38 386 68 897 248 1022 979
Cuts at: 4017 230
Size: 547
Fragments arranged by size:
1022 979 897 547 386 248 149 68
38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 390 422 692 721 724 753 2176 2206 2208
Size: 32 270 29 3 29 1423 30 2
Cuts at: 2208 2238 390
Size: 30 2486
Fragments arranged by size:
2486 1423 270 32 30 30 29 29
3 2
HincII GTy'rAC
Cuts at: 1373 1373
Size: 4334
KasI G'GCGC_C
Cuts at: 375 413 799 1764 2012 375
Size: 38 386 965 248 2697
Fragments arranged by size:
2697 965 386 248 38
KpnI G_GTAC'C
Cuts at: 1354 1354
Size: 4334
MluI A'CGCG_T
Cuts at: 339 3767 339
Size: 3428 906
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 125 3005 3189 125
Size: 2880 184 1270
Fragments arranged by size:
2880 1270 184
MscI TGG'CCA
Cuts at: 829 2095 829
Size: 1266 3068
MslI CAynn'nnrTG
Cuts at: 482 2323 2450 482
Size: 1841 127 2366
Fragments arranged by size:
2366 1841 127
MspA1I CmG'CkG
Cuts at: 160 441 864 1445 1759 2119 3132 3377 160
Size: 281 423 581 314 360 1013 245 1117
Fragments arranged by size:
1117 1013 581 423 360 314 281 245
NaeI GCC'GGC
Cuts at: 2515 2515
Size: 4334
NarI GG'CG_CC
Cuts at: 376 414 800 1765 2013 376
Size: 38 386 965 248 2697
Fragments arranged by size:
2697 965 386 248 38
NgoMIV G'CCGG_C
Cuts at: 2513 2513
Size: 4334
NspI r_CATG'y
Cuts at: 1387 1887 2794 1387
Size: 500 907 2927
Fragments arranged by size:
2927 907 500
PasI CC'CwG_GG
Cuts at: 521 521
Size: 4334
PciI A'CATG_T
Cuts at: 2790 2790
Size: 4334
PflMI CCAn_nnn'nTGG
Cuts at: 195 195
Size: 4334
PmeI GTTT'AAAC
Cuts at: 1333 1333
Size: 4334
PmlI CAC'GTG
Cuts at: 276 939 276
Size: 663 3671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 412 444 3473 3505 412
Size: 32 3029 32 1241
Fragments arranged by size:
3029 1241 32 32
PpuMI rG'GwC_Cy
Cuts at: 525 907 1197 525
Size: 382 290 3662
Fragments arranged by size:
3662 382 290
PspOMI G'GGCC_C
Cuts at: 852 852
Size: 4334
PspXI vC'TCGA_Gb
Cuts at: 306 306
Size: 4334
PstI C_TGCA'G
Cuts at: 1381 2066 1381
Size: 685 3649
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4334
PvuII CAG'CTG
Cuts at: 441 1759 2119 441
Size: 1318 360 2656
Fragments arranged by size:
2656 1318 360
RsrII CG'GwC_CG
Cuts at: 2529 2529
Size: 4334
SacI G_AGCT'C
Cuts at: 1348 1348
Size: 4334
SacII CC_GC'GG
Cuts at: 161 161
Size: 4334
SalI G'TCGA_C
Cuts at: 1371 1371
Size: 4334
SanDI GG'GwC_CC
Cuts at: 525 525
Size: 4334
SapI GCTCTTCn'nnn_
Cuts at: 2357 2567 2357
Size: 210 4124
SbfI CC_TGCA'GG
Cuts at: 1381 1381
Size: 4334
ScaI AGT'ACT
Cuts at: 1132 3851 1132
Size: 2719 1615
SfcI C'TryA_G
Cuts at: 33 330 1377 2062 3055 3246 33
Size: 297 1047 685 993 191 1121
Fragments arranged by size:
1121 1047 993 685 297 191
SfoI GGC'GCC
Cuts at: 377 415 801 1766 2014 377
Size: 38 386 965 248 2697
Fragments arranged by size:
2697 965 386 248 38
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
SmaI CCC'GGG
Cuts at: 858 1356 858
Size: 498 3836
SmlI C'TyrA_G
Cuts at: 306 602 952 1489 2404 2896 3158 306
Size: 296 350 537 915 492 262 1482
Fragments arranged by size:
1482 915 537 492 350 296 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4334
SphI G_CATG'C
Cuts at: 1387 1887 1387
Size: 500 3834
StyI C'CwwG_G
Cuts at: 1166 1468 1822 1166
Size: 302 354 3678
Fragments arranged by size:
3678 354 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 229 301 1917 2050 2509 2718 229
Size: 72 1616 133 459 209 1845
Fragments arranged by size:
1845 1616 459 209 133 72
TatI w'GTAC_w
Cuts at: 1130 1268 3849 1130
Size: 138 2581 1615
Fragments arranged by size:
2581 1615 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 878 910 3851 3883 878
Size: 32 2941 32 1329
Fragments arranged by size:
2941 1329 32 32
Tth111I GACn'n_nGTC
Cuts at: 288 2131 288
Size: 1843 2491
XbaI T'CTAG_A
Cuts at: 1365 1365
Size: 4334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 990 1077 1829 990
Size: 87 752 3495
Fragments arranged by size:
3495 752 87
XhoI C'TCGA_G
Cuts at: 306 306
Size: 4334
XmaI C'CCGG_G
Cuts at: 856 1354 856
Size: 498 3836
XmnI GAAnn'nnTTC
Cuts at: 130 550 130
Size: 420 3914
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BstEII BstZ17I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PfoI PshAI PsiI
PsrI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI ZraI