Restriction map for
pF1KB4878
(Circular) (Six-base) MAPSORT of: pF1KB4878.seq Check: 6988 from: 1 to: 4646
pF1KB4878 4646 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1684 1684
Size: 4646
Acc65I G'GTAC_C
Cuts at: 1662 1662
Size: 4646
AclI AA'CG_TT
Cuts at: 4486 4486
Size: 4646
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2471 2903 3256 3918 2471
Size: 432 353 662 3199
Fragments arranged by size:
3199 662 432 353
AflIII A'CryG_T
Cuts at: 1998 3798 4079 1998
Size: 1800 281 2565
Fragments arranged by size:
2565 1800 281
AleI CACnn'nnGTG
Cuts at: 670 1060 670
Size: 390 4256
AlwNI CAG_nnn'CTG
Cuts at: 1350 1951 3389 1350
Size: 601 1438 2607
Fragments arranged by size:
2607 1438 601
ApaLI G'TGCA_C
Cuts at: 3484 3484
Size: 4646
ApoI r'AATT_y
Cuts at: 660 724 1650 3999 4255 660
Size: 64 926 2349 256 1051
Fragments arranged by size:
2349 1051 926 256 64
AseI AT'TA_AT
Cuts at: 20 20
Size: 4646
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4646
AvaI C'yCGr_G
Cuts at: 1666 1666
Size: 4646
BamHI G'GATC_C
Cuts at: 1671 1671
Size: 4646
BanI G'GyrC_C
Cuts at: 1406 1447 1662 2076 2324 2359 1406
Size: 41 215 414 248 35 3693
Fragments arranged by size:
3693 414 248 215 41 35
BanII G_rGCy'C
Cuts at: 872 1040 1660 2690 872
Size: 168 620 1030 2828
Fragments arranged by size:
2828 1030 620 168
BbeI G_GCGC'C
Cuts at: 2080 2328 2080
Size: 248 4398
BbsI GAAGACnn'nnnn_
Cuts at: 783 913 1473 783
Size: 130 560 3956
Fragments arranged by size:
3956 560 130
BbvCI CC'TCA_GC
Cuts at: 655 655
Size: 4646
BciVI GTATCCnnnnn_n'
Cuts at: 2544 3600 4646 2544
Size: 1056 1046 2544
Fragments arranged by size:
2544 1056 1046
BclI T'GATC_A
Cuts at: 1418 2166 1418
Size: 748 3898
BglII A'GATC_T
Cuts at: 2161 4198 2161
Size: 2037 2609
BlpI GC'TnA_GC
Cuts at: 183 1758 183
Size: 1575 3071
Bme1580I G_kGCm'C
Cuts at: 1409 2271 2364 3488 1409
Size: 862 93 1124 2567
Fragments arranged by size:
2567 1124 862 93
BmrI ACTGGGnnnn_n'
Cuts at: 1412 1858 1939 2274 4418 1412
Size: 446 81 335 2144 1640
Fragments arranged by size:
2144 1640 446 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3013 3013
Size: 4646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 622
Size: 4646
Bpu10I CC'TnA_GC
Cuts at: 655 655
Size: 4646
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1389 1822 2701 3415 3713 1389
Size: 433 879 714 298 2322
Fragments arranged by size:
2322 879 714 433 298
BsaI GGTCTCn'nnnn_
Cuts at: 760 4350 760
Size: 3590 1056
BsaAI yAC'GTr
Cuts at: 1999 1999
Size: 4646
BsaBI GATnn'nnATC
Cuts at: 6 2160 4197 6
Size: 2154 2037 455
Fragments arranged by size:
2154 2037 455
BsaHI Gr'CG_yC
Cuts at: 2077 2325 3027 4523 2077
Size: 248 702 1496 2200
Fragments arranged by size:
2200 1496 702 248
BsaWI w'CCGG_w
Cuts at: 794 1835 2356 3034 3445 3592 3866 794
Size: 1041 521 678 411 147 274 1574
Fragments arranged by size:
1574 1041 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 289
Size: 4646
BseYI C'CCAG_C
Cuts at: 243 352 677 1434 2071 2829 3494 3902 243
Size: 109 325 757 637 758 665 408 987
Fragments arranged by size:
987 758 757 665 637 408 325 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 643
Size: 4646
BsiEI CG_ry'CG
Cuts at: 104 2188 2234 3464 104
Size: 2084 46 1230 1286
Fragments arranged by size:
2084 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 830 872 884 1660 2438 2628 3488 830
Size: 42 12 776 778 190 860 1988
Fragments arranged by size:
1988 860 778 776 190 42 12
Bsp1286I G_dGCh'C
Cuts at: 830 872 884 1040 1409 1660 2271 2364 2438
Size: 42 12 156 369 251 611 93 74
Cuts at: 2438 2628 2690 3488 830
Size: 190 62 798 1988
Fragments arranged by size:
1988 798 611 369 251 190 156 93
74 62 42 12
BspEI T'CCGG_A
Cuts at: 794 794
Size: 4646
BspHI T'CATG_A
Cuts at: 296 3078 296
Size: 2782 1864
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1696 1910 2212 2593 1696
Size: 214 302 381 3749
Fragments arranged by size:
3749 381 302 214
BsrBI CCG'CTC
Cuts at: 50 132 2938 2992 4476 4641 50
Size: 82 2806 54 1484 165 55
Fragments arranged by size:
2806 1484 165 82 55 54
BsrDI GCAATG_nn'
Cuts at: 173 2558 173
Size: 2385 2261
BsrFI r'CCGG_y
Cuts at: 858 2644 2825 858
Size: 1786 181 2679
Fragments arranged by size:
2679 1786 181
BssSI C'ACGA_G
Cuts at: 2917 3625 2917
Size: 708 3938
BstAPI GCAn_nnn'nTGC
Cuts at: 2023 2023
Size: 4646
BstBI TT'CG_AA
Cuts at: 3007 3007
Size: 4646
BstEII G'GTnAC_C
Cuts at: 672 672
Size: 4646
BstXI CCAn_nnnn'nTGG
Cuts at: 114 454 999 1026 1062 1395 114
Size: 340 545 27 36 333 3365
Fragments arranged by size:
3365 545 340 333 36 27
BstYI r'GATC_y
Cuts at: 1 988 1671 2138 2161 2496 2742 3048 3060
Size: 987 683 467 23 335 246 306 12
Cuts at: 3060 3146 4198 1
Size: 86 1052 449
Fragments arranged by size:
1052 987 683 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1513 1513
Size: 4646
BtgI C'CryG_G
Cuts at: 1031 1055 1257 1412 1031
Size: 24 202 155 4265
Fragments arranged by size:
4265 202 155 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 993 2596 2777 2940 993
Size: 1603 181 163 2699
Fragments arranged by size:
2699 1603 181 163
BtsI GCAGTG_nn'
Cuts at: 504 1889 504
Size: 1385 3261
DraI TTT'AAA
Cuts at: 1645 1645
Size: 4646
DrdI GACnn_nn'nnGTC
Cuts at: 2352 3696 2352
Size: 1344 3302
EaeI y'GGCC_r
Cuts at: 133 281 1028 1098 1254 1397 1532 2231 2405
Size: 148 747 70 156 143 135 699 174
Cuts at: 2405 2796 2823 4168 133
Size: 391 27 1345 611
Fragments arranged by size:
1345 747 699 611 391 174 156 148
143 135 70 27
EagI C'GGCC_G
Cuts at: 2231 2231
Size: 4646
EarI CTCTTCn'nnn_
Cuts at: 887 1597 2669 2879 887
Size: 710 1072 210 2654
Fragments arranged by size:
2654 1072 710 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2854 3596 3742 4456 2854
Size: 742 146 714 3044
Fragments arranged by size:
3044 742 714 146
EcoICRI GAG'CTC
Cuts at: 870 1658 870
Size: 788 3858
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 2471 2903 3256 3918 622
Size: 1849 432 353 662 1350
Fragments arranged by size:
1849 1350 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 335 1517 1785 335
Size: 1182 268 3196
Fragments arranged by size:
3196 1182 268
EcoRI G'AATT_C
Cuts at: 1650 1650
Size: 4646
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2665 2665
Size: 4646
FspI TGC'GCA
Cuts at: 2427 2427
Size: 4646
HaeII r_GCGC'y
Cuts at: 2080 2328 3558 4329 2080
Size: 248 1230 771 2397
Fragments arranged by size:
2397 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 109 141 773 805 973 1005 2488 2518 2520
Size: 32 632 32 168 32 1483 30 2
Cuts at: 2520 2550 109
Size: 30 2205
Fragments arranged by size:
2205 1483 632 168 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1685 1685
Size: 4646
KasI G'GCGC_C
Cuts at: 2076 2324 2076
Size: 248 4398
KpnI G_GTAC'C
Cuts at: 1666 1666
Size: 4646
MluI A'CGCG_T
Cuts at: 4079 4079
Size: 4646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1544 3405 3589 1544
Size: 1861 184 2601
Fragments arranged by size:
2601 1861 184
MscI TGG'CCA
Cuts at: 283 1030 1100 1399 1534 2407 283
Size: 747 70 299 135 873 2522
Fragments arranged by size:
2522 873 747 299 135 70
MslI CAynn'nnrTG
Cuts at: 112 446 670 1024 1060 1393 2635 2762 112
Size: 334 224 354 36 333 1242 127 1996
Fragments arranged by size:
1996 1242 354 334 333 224 127 36
MspA1I CmG'CkG
Cuts at: 1530 1757 2071 2431 3215 3460 1530
Size: 227 314 360 784 245 2716
Fragments arranged by size:
2716 784 360 314 245 227
NaeI GCC'GGC
Cuts at: 2827 2827
Size: 4646
NarI GG'CG_CC
Cuts at: 2077 2325 2077
Size: 248 4398
NcoI C'CATG_G
Cuts at: 1031 1257 1412 1031
Size: 226 155 4265
Fragments arranged by size:
4265 226 155
NgoMIV G'CCGG_C
Cuts at: 2825 2825
Size: 4646
NspI r_CATG'y
Cuts at: 360 804 1699 2199 3802 360
Size: 444 895 500 1603 1204
Fragments arranged by size:
1603 1204 895 500 444
PciI A'CATG_T
Cuts at: 3798 3798
Size: 4646
PfoI T'CCnGG_A
Cuts at: 1300 1300
Size: 4646
PmeI GTTT'AAAC
Cuts at: 1645 1645
Size: 4646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 4614
PpuMI rG'GwC_Cy
Cuts at: 335 1517 335
Size: 1182 3464
PstI C_TGCA'G
Cuts at: 1693 2378 1693
Size: 685 3961
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4646
PvuII CAG'CTG
Cuts at: 1530 2071 2431 1530
Size: 541 360 3745
Fragments arranged by size:
3745 541 360
RsrII CG'GwC_CG
Cuts at: 2841 2841
Size: 4646
SacI G_AGCT'C
Cuts at: 872 1660 872
Size: 788 3858
SalI G'TCGA_C
Cuts at: 1683 1683
Size: 4646
SapI GCTCTTCn'nnn_
Cuts at: 2669 2879 2669
Size: 210 4436
SbfI CC_TGCA'GG
Cuts at: 1693 1693
Size: 4646
ScaI AGT'ACT
Cuts at: 4163 4163
Size: 4646
SexAI A'CCwGG_T
Cuts at: 1076 1343 1076
Size: 267 4379
SfcI C'TryA_G
Cuts at: 33 495 1382 1689 2374 3342 3533 33
Size: 462 887 307 685 968 191 1146
Fragments arranged by size:
1146 968 887 685 462 307 191
SfoI GGC'GCC
Cuts at: 2078 2326 2078
Size: 248 4398
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4646
SmaI CCC'GGG
Cuts at: 1668 1668
Size: 4646
SmlI C'TyrA_G
Cuts at: 1368 1801 2716 3430 3692 1368
Size: 433 915 714 262 2322
Fragments arranged by size:
2322 915 714 433 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4646
SphI G_CATG'C
Cuts at: 360 804 1699 2199 360
Size: 444 895 500 2807
Fragments arranged by size:
2807 895 500 444
SspI AAT'ATT
Cuts at: 408 408
Size: 4646
StuI AGG'CCT
Cuts at: 501 501
Size: 4646
StyI C'CwwG_G
Cuts at: 269 526 1031 1142 1257 1412 1780 2134 269
Size: 257 505 111 115 155 368 354 2781
Fragments arranged by size:
2781 505 368 354 257 155 115 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 366 572 2229 2362 2821 3030 366
Size: 206 1657 133 459 209 1982
Fragments arranged by size:
1982 1657 459 209 206 133
TatI w'GTAC_w
Cuts at: 1550 4161 1550
Size: 2611 2035
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4163 4195 4163
Size: 32 4614
Tth111I GACn'n_nGTC
Cuts at: 787 2443 787
Size: 1656 2990
XbaI T'CTAG_A
Cuts at: 1677 1677
Size: 4646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 276 2141 276
Size: 1865 2781
XmaI C'CCGG_G
Cuts at: 1666 1666
Size: 4646
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI