Restriction map for
pF1KB4865
(Circular) (Six-base) MAPSORT of: pF1KB4865.seq Check: 6058 from: 1 to: 5882
pF1KB4865 5882 bp
With 182 enzymes: SgfI *
March 1, 2013 10:20 ..
AccI GT'mk_AC
Cuts at: 1091 1370 2920 1091
Size: 279 1550 4053
Fragments arranged by size:
4053 1550 279
Acc65I G'GTAC_C
Cuts at: 218 2898 218
Size: 2680 3202
AclI AA'CG_TT
Cuts at: 5722 5722
Size: 5882
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 3707 4139 4886 5154 434
Size: 3273 432 747 268 1162
Fragments arranged by size:
3273 1162 747 432 268
AfeI AGC'GCT
Cuts at: 1021 1021
Size: 5882
AflIII A'CryG_T
Cuts at: 2288 3234 4338 5315 2288
Size: 946 1104 977 2855
Fragments arranged by size:
2855 1104 977 946
AgeI A'CCGG_T
Cuts at: 4270 4270
Size: 5882
AleI CACnn'nnGTG
Cuts at: 257 1981 2384 257
Size: 1724 403 3755
Fragments arranged by size:
3755 1724 403
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1999 1999
Size: 5882
AlwNI CAG_nnn'CTG
Cuts at: 350 1475 3187 4754 350
Size: 1125 1712 1567 1478
Fragments arranged by size:
1712 1567 1478 1125
ApaLI G'TGCA_C
Cuts at: 4652 4652
Size: 5882
ApoI r'AATT_y
Cuts at: 472 524 670 962 2059 2886 5235 5491 472
Size: 52 146 292 1097 827 2349 256 863
Fragments arranged by size:
2349 1097 863 827 292 256 146 52
AseI AT'TA_AT
Cuts at: 20 2507 20
Size: 2487 3395
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5882
AvaI C'yCGr_G
Cuts at: 214 1804 2902 214
Size: 1590 1098 3194
Fragments arranged by size:
3194 1590 1098
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2595 2628 2595
Size: 33 5849
BamHI G'GATC_C
Cuts at: 2907 2907
Size: 5882
BanI G'GyrC_C
Cuts at: 218 1135 1975 2898 3312 3560 3595 218
Size: 917 840 923 414 248 35 2505
Fragments arranged by size:
2505 923 917 840 414 248 35
BanII G_rGCy'C
Cuts at: 1835 2074 2239 2896 3926 1835
Size: 239 165 657 1030 3791
Fragments arranged by size:
3791 1030 657 239 165
BbeI G_GCGC'C
Cuts at: 1139 3316 3564 1139
Size: 2177 248 3457
Fragments arranged by size:
3457 2177 248
BbsI GAAGACnn'nnnn_
Cuts at: 366 440 1214 2668 366
Size: 74 774 1454 3580
Fragments arranged by size:
3580 1454 774 74
BciVI GTATCCnnnnn_n'
Cuts at: 1076 2596 3780 4541 5882 1076
Size: 1520 1184 761 1341 1076
Fragments arranged by size:
1520 1341 1184 1076 761
BclI T'GATC_A
Cuts at: 311 1691 3402 311
Size: 1380 1711 2791
Fragments arranged by size:
2791 1711 1380
BglII A'GATC_T
Cuts at: 3397 5434 3397
Size: 2037 3845
BlpI GC'TnA_GC
Cuts at: 2994 2994
Size: 5882
Bme1580I G_kGCm'C
Cuts at: 1524 2706 3507 3600 4656 1524
Size: 1182 801 93 1056 2750
Fragments arranged by size:
2750 1182 1056 801 93
BmgBI CAC'GTC
Cuts at: 2304 2304
Size: 5882
BmrI ACTGGGnnnn_n'
Cuts at: 987 1837 2103 3094 3175 3510 5654 987
Size: 850 266 991 81 335 2144 1215
Fragments arranged by size:
2144 1215 991 850 335 266 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 714 1832 4249 714
Size: 1118 2417 2347
Fragments arranged by size:
2417 2347 1118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 692 709 2622 2815 692
Size: 17 1913 193 3759
Fragments arranged by size:
3759 1913 193 17
Bpu10I CC'TnA_GC
Cuts at: 1754 1754
Size: 5882
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2104 3058 3937 4429 4727 2104
Size: 954 879 492 298 3259
Fragments arranged by size:
3259 954 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5586 5586
Size: 5882
BsaAI yAC'GTr
Cuts at: 366 2112 3235 366
Size: 1746 1123 3013
Fragments arranged by size:
3013 1746 1123
BsaBI GATnn'nnATC
Cuts at: 6 822 3396 5433 6
Size: 816 2574 2037 455
Fragments arranged by size:
2574 2037 816 455
BsaHI Gr'CG_yC
Cuts at: 1136 3313 3561 4263 5759 1136
Size: 2177 248 702 1496 1259
Fragments arranged by size:
2177 1496 1259 702 248
BsaWI w'CCGG_w
Cuts at: 288 3071 3592 4270 4544 4691 5102 288
Size: 2783 521 678 274 147 411 1068
Fragments arranged by size:
2783 1068 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1180 1210 1180
Size: 30 5852
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 118 425 1594 2359 118
Size: 307 1169 765 3641
Fragments arranged by size:
3641 1169 765 307
BseYI C'CCAG_C
Cuts at: 1517 1848 2066 3307 4065 4642 5138 1517
Size: 331 218 1241 758 577 496 2261
Fragments arranged by size:
2261 1241 758 577 496 331 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 2134 521
Size: 1613 4269
BsiEI CG_ry'CG
Cuts at: 104 164 735 3424 3470 4678 104
Size: 60 571 2689 46 1208 1308
Fragments arranged by size:
2689 1308 1208 571 60 46
BsiHKAI G_wGCw'C
Cuts at: 143 2596 2896 3674 3864 4656 143
Size: 2453 300 778 190 792 1369
Fragments arranged by size:
2453 1369 792 778 300 190
BsmI GAATG_Cn'
Cuts at: 628 830 628
Size: 202 5680
Bsp1286I G_dGCh'C
Cuts at: 143 1524 1835 2074 2239 2596 2706 2896 3507
Size: 1381 311 239 165 357 110 190 611
Cuts at: 3507 3600 3674 3864 3926 4656 143
Size: 93 74 190 62 730 1369
Fragments arranged by size:
1381 1369 730 611 357 311 239 190
190 165 110 93 74 62
BspEI T'CCGG_A
Cuts at: 288 5102 288
Size: 4814 1068
BspHI T'CATG_A
Cuts at: 1640 5058 1640
Size: 3418 2464
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2932 3146 3448 3829 2932
Size: 214 302 381 4985
Fragments arranged by size:
4985 381 302 214
BsrBI CCG'CTC
Cuts at: 50 126 4174 4228 5712 5877 50
Size: 76 4048 54 1484 165 55
Fragments arranged by size:
4048 1484 165 76 55 54
BsrDI GCAATG_nn'
Cuts at: 2658 3794 2658
Size: 1136 4746
BsrFI r'CCGG_y
Cuts at: 163 734 3880 4061 4270 163
Size: 571 3146 181 209 1775
Fragments arranged by size:
3146 1775 571 209 181
BssSI C'ACGA_G
Cuts at: 4153 4511 4153
Size: 358 5524
BstAPI GCAn_nnn'nTGC
Cuts at: 3259 3259
Size: 5882
BstBI TT'CG_AA
Cuts at: 4243 4243
Size: 5882
BstEII G'GTnAC_C
Cuts at: 1500 2386 1500
Size: 886 4996
BstXI CCAn_nnnn'nTGG
Cuts at: 1986 1986
Size: 5882
BstYI r'GATC_y
Cuts at: 1 1796 2907 3374 3397 3732 3978 4990 5076
Size: 1795 1111 467 23 335 246 1012 86
Cuts at: 5076 5088 5434 1
Size: 12 346 449
Fragments arranged by size:
1795 1111 1012 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1371 1371
Size: 5882
BtgI C'CryG_G
Cuts at: 107 839 2003 107
Size: 732 1164 3986
Fragments arranged by size:
3986 1164 732
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3832 4013 4176 3832
Size: 181 163 5538
Fragments arranged by size:
5538 181 163
BtsI GCAGTG_nn'
Cuts at: 915 2265 3125 915
Size: 1350 860 3672
Fragments arranged by size:
3672 1350 860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1257 1292 1257
Size: 35 5847
DraI TTT'AAA
Cuts at: 2881 2881
Size: 5882
DraIII CAC_nnn'GTG
Cuts at: 499 571 499
Size: 72 5810
DrdI GACnn_nn'nnGTC
Cuts at: 3588 4446 3588
Size: 858 5024
EaeI y'GGCC_r
Cuts at: 161 1988 2369 3467 3641 4032 4059 5404 161
Size: 1827 381 1098 174 391 27 1345 639
Fragments arranged by size:
1827 1345 1098 639 391 381 174 27
EagI C'GGCC_G
Cuts at: 161 3467 161
Size: 3306 2576
EarI CTCTTCn'nnn_
Cuts at: 3905 4115 3905
Size: 210 5672
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1483 1838 4090 4400 4546 5692 1483
Size: 355 2252 310 146 1146 1673
Fragments arranged by size:
2252 1673 1146 355 310 146
EcoICRI GAG'CTC
Cuts at: 2894 2894
Size: 5882
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 692 709 2622 2815 3707 4139 4886 5154
Size: 258 17 1913 193 892 432 747 268
Cuts at: 5154 434
Size: 1162
Fragments arranged by size:
1913 1162 892 747 432 268 258 193
17
EcoNI CCTnn'n_nnAGG
Cuts at: 1576 1576
Size: 5882
EcoO109I rG'GnC_Cy
Cuts at: 538 2149 2297 2712 3021 538
Size: 1611 148 415 309 3399
Fragments arranged by size:
3399 1611 415 309 148
EcoRI G'AATT_C
Cuts at: 2886 2886
Size: 5882
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2491 3901 2491
Size: 1410 4472
FspI TGC'GCA
Cuts at: 3663 3663
Size: 5882
HaeII r_GCGC'y
Cuts at: 170 1023 1139 3316 3564 4586 5565 170
Size: 853 116 2177 248 1022 979 487
Fragments arranged by size:
2177 1022 979 853 487 248 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 270 302 417 449 708 740 1180 1212 1817
Size: 32 115 32 259 32 440 32 605
Cuts at: 1817 1849 3724 3754 3756 3786 270
Size: 32 1875 30 2 30 2366
Fragments arranged by size:
2366 1875 605 440 259 115 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2135 2921 2135
Size: 786 5096
HindIII A'AGCT_T
Cuts at: 678 678
Size: 5882
KasI G'GCGC_C
Cuts at: 1135 3312 3560 1135
Size: 2177 248 3457
Fragments arranged by size:
3457 2177 248
KpnI G_GTAC'C
Cuts at: 222 2902 222
Size: 2680 3202
MluI A'CGCG_T
Cuts at: 5315 5315
Size: 5882
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1223 1481 2062 4553 4737 1223
Size: 258 581 2491 184 2368
Fragments arranged by size:
2491 2368 581 258 184
MscI TGG'CCA
Cuts at: 1990 2371 3643 1990
Size: 381 1272 4229
Fragments arranged by size:
4229 1272 381
MslI CAynn'nnrTG
Cuts at: 257 1354 1981 2384 2695 3871 3998 257
Size: 1097 627 403 311 1176 127 2141
Fragments arranged by size:
2141 1176 1097 627 403 311 127
MspA1I CmG'CkG
Cuts at: 1876 2358 2993 3307 3667 4680 4925 1876
Size: 482 635 314 360 1013 245 2833
Fragments arranged by size:
2833 1013 635 482 360 314 245
NaeI GCC'GGC
Cuts at: 165 4063 165
Size: 3898 1984
NarI GG'CG_CC
Cuts at: 1136 3313 3561 1136
Size: 2177 248 3457
Fragments arranged by size:
3457 2177 248
NcoI C'CATG_G
Cuts at: 107 839 107
Size: 732 5150
NdeI CA'TA_TG
Cuts at: 2043 2043
Size: 5882
NgoMIV G'CCGG_C
Cuts at: 163 4061 163
Size: 3898 1984
NspI r_CATG'y
Cuts at: 2292 2935 3435 4342 2292
Size: 643 500 907 3832
Fragments arranged by size:
3832 907 643 500
PasI CC'CwG_GG
Cuts at: 2706 2706
Size: 5882
PciI A'CATG_T
Cuts at: 2288 4338 2288
Size: 2050 3832
PflMI CCAn_nnn'nTGG
Cuts at: 2044 2044
Size: 5882
PfoI T'CCnGG_A
Cuts at: 665 1056 2638 665
Size: 391 1582 3909
Fragments arranged by size:
3909 1582 391
PmeI GTTT'AAAC
Cuts at: 2881 2881
Size: 5882
PmlI CAC'GTG
Cuts at: 2112 2112
Size: 5882
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5021 5053 5021
Size: 32 5850
PpuMI rG'GwC_Cy
Cuts at: 538 2149 2297 2712 538
Size: 1611 148 415 3708
Fragments arranged by size:
3708 1611 415 148
PstI C_TGCA'G
Cuts at: 1017 2929 3614 1017
Size: 1912 685 3285
Fragments arranged by size:
3285 1912 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5882
PvuII CAG'CTG
Cuts at: 1876 2358 3307 3667 1876
Size: 482 949 360 4091
Fragments arranged by size:
4091 949 482 360
RsrII CG'GwC_CG
Cuts at: 4077 4077
Size: 5882
SacI G_AGCT'C
Cuts at: 2896 2896
Size: 5882
SalI G'TCGA_C
Cuts at: 2919 2919
Size: 5882
SanDI GG'GwC_CC
Cuts at: 2149 2297 2149
Size: 148 5734
SapI GCTCTTCn'nnn_
Cuts at: 3905 4115 3905
Size: 210 5672
SbfI CC_TGCA'GG
Cuts at: 2929 2929
Size: 5882
ScaI AGT'ACT
Cuts at: 2862 5399 2862
Size: 2537 3345
SexAI A'CCwGG_T
Cuts at: 804 804
Size: 5882
SfcI C'TryA_G
Cuts at: 33 891 1013 1871 2925 3610 4603 4794 33
Size: 858 122 858 1054 685 993 191 1121
Fragments arranged by size:
1121 1054 993 858 858 685 191 122
SfoI GGC'GCC
Cuts at: 1137 3314 3562 1137
Size: 2177 248 3457
Fragments arranged by size:
3457 2177 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5882
SmaI CCC'GGG
Cuts at: 216 2904 216
Size: 2688 3194
SmlI C'TyrA_G
Cuts at: 2119 3037 3952 4444 4706 2119
Size: 918 915 492 262 3295
Fragments arranged by size:
3295 918 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5882
SphI G_CATG'C
Cuts at: 2935 3435 2935
Size: 500 5382
SspI AAT'ATT
Cuts at: 1165 1165
Size: 5882
StyI C'CwwG_G
Cuts at: 107 839 1461 2382 2475 3016 3370 107
Size: 732 622 921 93 541 354 2619
Fragments arranged by size:
2619 921 732 622 541 354 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 269 1037 1896 3465 3598 4057 4266 269
Size: 768 859 1569 133 459 209 1885
Fragments arranged by size:
1885 1569 859 768 459 209 133
TatI w'GTAC_w
Cuts at: 2744 2860 5397 2744
Size: 116 2537 3229
Fragments arranged by size:
3229 2537 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5399 5431 5399
Size: 32 5850
Tth111I GACn'n_nGTC
Cuts at: 3679 3679
Size: 5882
XbaI T'CTAG_A
Cuts at: 2913 2913
Size: 5882
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1275 1899 1969 2703 3377 1275
Size: 624 70 734 674 3780
Fragments arranged by size:
3780 734 674 624 70
XmaI C'CCGG_G
Cuts at: 214 2902 214
Size: 2688 3194
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BbvCI BcgI BfrBI BglI BmtI BsiWI BsmBI BsrGI
BssHII Bsu36I ClaI EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI NsiI PacI PshAI PsiI PspOMI
PspXI PsrI SacII SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI