Restriction map for
pF1KB4846
(Circular) (Six-base) MAPSORT of: pF1KB4846.seq Check: 3985 from: 1 to: 4553
pF1KB4846 4553 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AatII G_ACGT'C
Cuts at: 200 200
Size: 4553
AccI GT'mk_AC
Cuts at: 1065 1591 1065
Size: 526 4027
Acc65I G'GTAC_C
Cuts at: 1569 1569
Size: 4553
AclI AA'CG_TT
Cuts at: 4393 4393
Size: 4553
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 2378 2810 3163 3825 98
Size: 2280 432 353 662 826
Fragments arranged by size:
2280 826 662 432 353
AfeI AGC'GCT
Cuts at: 1375 1375
Size: 4553
AflIII A'CryG_T
Cuts at: 1905 3705 3986 1905
Size: 1800 281 2472
Fragments arranged by size:
2472 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1858 3296 1858
Size: 1438 3115
ApaI G_GGCC'C
Cuts at: 707 707
Size: 4553
ApaLI G'TGCA_C
Cuts at: 994 3391 994
Size: 2397 2156
ApoI r'AATT_y
Cuts at: 1557 3906 4162 1557
Size: 2349 256 1948
Fragments arranged by size:
2349 1948 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4553
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
AvaI C'yCGr_G
Cuts at: 363 707 754 1191 1573 363
Size: 344 47 437 382 3343
Fragments arranged by size:
3343 437 382 344 47
AvrII C'CTAG_G
Cuts at: 468 468
Size: 4553
BamHI G'GATC_C
Cuts at: 973 1578 973
Size: 605 3948
BanI G'GyrC_C
Cuts at: 283 1077 1159 1330 1506 1569 1983 2231 2266
Size: 794 82 171 176 63 414 248 35
Cuts at: 2266 283
Size: 2570
Fragments arranged by size:
2570 794 414 248 176 171 82 63
35
BanII G_rGCy'C
Cuts at: 253 707 1225 1567 2597 253
Size: 454 518 342 1030 2209
Fragments arranged by size:
2209 1030 518 454 342
BbeI G_GCGC'C
Cuts at: 287 1163 1334 1510 1987 2235 287
Size: 876 171 176 477 248 2605
Fragments arranged by size:
2605 876 477 248 176 171
BbsI GAAGACnn'nnnn_
Cuts at: 636 726 636
Size: 90 4463
BbvCI CC'TCA_GC
Cuts at: 319 890 319
Size: 571 3982
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1185 1219 1185
Size: 34 4519
BciVI GTATCCnnnnn_n'
Cuts at: 906 2451 3507 4553 906
Size: 1545 1056 1046 906
Fragments arranged by size:
1545 1056 1046 906
BclI T'GATC_A
Cuts at: 741 2073 741
Size: 1332 3221
BfrBI ATG'CAT
Cuts at: 170 170
Size: 4553
BglII A'GATC_T
Cuts at: 2068 4105 2068
Size: 2037 2516
BlpI GC'TnA_GC
Cuts at: 247 533 1665 247
Size: 286 1132 3135
Fragments arranged by size:
3135 1132 286
Bme1580I G_kGCm'C
Cuts at: 707 998 2178 2271 3395 707
Size: 291 1180 93 1124 1865
Fragments arranged by size:
1865 1180 1124 291 93
BmgBI CAC'GTC
Cuts at: 1024 1461 1024
Size: 437 4116
BmrI ACTGGGnnnn_n'
Cuts at: 1765 1846 2181 4325 1765
Size: 81 335 2144 1993
Fragments arranged by size:
2144 1993 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 4553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1543 811
Size: 732 3821
Bpu10I CC'TnA_GC
Cuts at: 319 890 319
Size: 571 3982
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1729 2608 3322 3620 1729
Size: 879 714 298 2662
Fragments arranged by size:
2662 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4257 4257
Size: 4553
BsaAI yAC'GTr
Cuts at: 1906 1906
Size: 4553
BsaBI GATnn'nnATC
Cuts at: 6 2067 4104 6
Size: 2061 2037 455
Fragments arranged by size:
2061 2037 455
BsaHI Gr'CG_yC
Cuts at: 197 271 284 293 1160 1180 1331 1507 1984
Size: 74 13 9 867 20 151 176 477
Cuts at: 1984 2232 2934 4430 197
Size: 248 702 1496 320
Fragments arranged by size:
1496 867 702 477 320 248 176 151
74 20 13 9
BsaWI w'CCGG_w
Cuts at: 1742 2263 2941 3352 3499 3773 1742
Size: 521 678 411 147 274 2522
Fragments arranged by size:
2522 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1339 1369 1474 1504 1519 1549 1339
Size: 30 105 30 15 30 4343
Fragments arranged by size:
4343 105 30 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 336 1540 336
Size: 1204 3349
BseYI C'CCAG_C
Cuts at: 279 834 999 1438 1978 2736 3401 3809 279
Size: 555 165 439 540 758 665 408 1023
Fragments arranged by size:
1023 758 665 555 540 439 408 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1386 1386
Size: 4553
BsiEI CG_ry'CG
Cuts at: 104 333 603 1340 2095 2141 3371 104
Size: 229 270 737 755 46 1230 1286
Fragments arranged by size:
1286 1230 755 737 270 229 46
BsiHKAI G_wGCw'C
Cuts at: 998 1567 2345 2535 3395 998
Size: 569 778 190 860 2156
Fragments arranged by size:
2156 860 778 569 190
BsmI GAATG_Cn'
Cuts at: 172 172
Size: 4553
BsmBI CGTCTCn'nnnn_
Cuts at: 188 188
Size: 4553
Bsp1286I G_dGCh'C
Cuts at: 253 707 998 1225 1567 2178 2271 2345 2535
Size: 454 291 227 342 611 93 74 190
Cuts at: 2535 2597 3395 253
Size: 62 798 1411
Fragments arranged by size:
1411 798 611 454 342 291 227 190
93 74 62
BspHI T'CATG_A
Cuts at: 158 2985 158
Size: 2827 1726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1256 1603 1817 2119 2500 1256
Size: 347 214 302 381 3309
Fragments arranged by size:
3309 381 347 302 214
BsrBI CCG'CTC
Cuts at: 50 326 777 2845 2899 4383 4548 50
Size: 276 451 2068 54 1484 165 55
Fragments arranged by size:
2068 1484 451 276 165 55 54
BsrDI GCAATG_nn'
Cuts at: 519 977 2465 519
Size: 458 1488 2607
Fragments arranged by size:
2607 1488 458
BsrFI r'CCGG_y
Cuts at: 295 310 1182 1339 1417 2551 2732 295
Size: 15 872 157 78 1134 181 2116
Fragments arranged by size:
2116 1134 872 181 157 78 15
BssHII G'CGCG_C
Cuts at: 1516 1516
Size: 4553
BssSI C'ACGA_G
Cuts at: 213 854 2824 3532 213
Size: 641 1970 708 1234
Fragments arranged by size:
1970 1234 708 641
BstAPI GCAn_nnn'nTGC
Cuts at: 1930 1930
Size: 4553
BstBI TT'CG_AA
Cuts at: 2914 2914
Size: 4553
BstEII G'GTnAC_C
Cuts at: 1185 1185
Size: 4553
BstXI CCAn_nnnn'nTGG
Cuts at: 1503 1503
Size: 4553
BstYI r'GATC_y
Cuts at: 1 923 973 1578 2045 2068 2403 2649 2955
Size: 922 50 605 467 23 335 246 306
Cuts at: 2955 2967 3053 4105 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 922 605 467 449 335 306 246
86 50 23 12
Bsu36I CC'TnA_GG
Cuts at: 589 589
Size: 4553
BtgI C'CryG_G
Cuts at: 107 940 1217 1358 107
Size: 833 277 141 3302
Fragments arranged by size:
3302 833 277 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 432 2503 2684 2847 432
Size: 2071 181 163 2138
Fragments arranged by size:
2138 2071 181 163
BtsI GCAGTG_nn'
Cuts at: 875 1796 875
Size: 921 3632
DraI TTT'AAA
Cuts at: 1552 1552
Size: 4553
DrdI GACnn_nn'nnGTC
Cuts at: 2259 3603 2259
Size: 1344 3209
EaeI y'GGCC_r
Cuts at: 308 353 1214 1226 1337 2138 2312 2703 2730
Size: 45 861 12 111 801 174 391 27
Cuts at: 2730 4075 308
Size: 1345 786
Fragments arranged by size:
1345 861 801 786 391 174 111 45
27 12
EagI C'GGCC_G
Cuts at: 1337 2138 1337
Size: 801 3752
EarI CTCTTCn'nnn_
Cuts at: 606 2576 2786 606
Size: 1970 210 2373
Fragments arranged by size:
2373 1970 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2761 3503 3649 4363 2761
Size: 742 146 714 2951
Fragments arranged by size:
2951 742 714 146
EcoICRI GAG'CTC
Cuts at: 1565 1565
Size: 4553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 811 1543 2378 2810 3163 3825 98
Size: 713 732 835 432 353 662 826
Fragments arranged by size:
835 826 732 713 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 1525 1525
Size: 4553
EcoO109I rG'GnC_Cy
Cuts at: 704 1541 1692 704
Size: 837 151 3565
Fragments arranged by size:
3565 837 151
EcoRI G'AATT_C
Cuts at: 1557 1557
Size: 4553
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4553
FspI TGC'GCA
Cuts at: 379 2334 379
Size: 1955 2598
FspAI rTGC'GCAy
Cuts at: 379 379
Size: 4553
HaeII r_GCGC'y
Cuts at: 287 302 429 1163 1334 1377 1510 1987 2235
Size: 15 127 734 171 43 133 477 248
Cuts at: 2235 3465 4236 287
Size: 1230 771 604
Fragments arranged by size:
1230 771 734 604 477 248 171 133
127 43 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2395 2425 2427 2457 2395
Size: 30 2 30 4491
Fragments arranged by size:
4491 30 30 2
HincII GTy'rAC
Cuts at: 1066 1592 1066
Size: 526 4027
HindIII A'AGCT_T
Cuts at: 621 621
Size: 4553
KasI G'GCGC_C
Cuts at: 283 1159 1330 1506 1983 2231 283
Size: 876 171 176 477 248 2605
Fragments arranged by size:
2605 876 477 248 176 171
KpnI G_GTAC'C
Cuts at: 1573 1573
Size: 4553
MluI A'CGCG_T
Cuts at: 3986 3986
Size: 4553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 951 1160 3312 3496 951
Size: 209 2152 184 2008
Fragments arranged by size:
2152 2008 209 184
MscI TGG'CCA
Cuts at: 2314 2314
Size: 4553
MslI CAynn'nnrTG
Cuts at: 502 811 1207 1501 2542 2669 502
Size: 309 396 294 1041 127 2386
Fragments arranged by size:
2386 1041 396 309 294 127
MspA1I CmG'CkG
Cuts at: 279 798 1031 1664 1978 2338 3122 3367 279
Size: 519 233 633 314 360 784 245 1465
Fragments arranged by size:
1465 784 633 519 360 314 245 233
NaeI GCC'GGC
Cuts at: 297 312 1341 2734 297
Size: 15 1029 1393 2116
Fragments arranged by size:
2116 1393 1029 15
NarI GG'CG_CC
Cuts at: 284 1160 1331 1507 1984 2232 284
Size: 876 171 176 477 248 2605
Fragments arranged by size:
2605 876 477 248 176 171
NcoI C'CATG_G
Cuts at: 107 1217 1358 107
Size: 1110 141 3302
Fragments arranged by size:
3302 1110 141
NgoMIV G'CCGG_C
Cuts at: 295 310 1339 2732 295
Size: 15 1029 1393 2116
Fragments arranged by size:
2116 1393 1029 15
NsiI A_TGCA'T
Cuts at: 172 172
Size: 4553
NspI r_CATG'y
Cuts at: 1149 1212 1606 2106 3709 1149
Size: 63 394 500 1603 1993
Fragments arranged by size:
1993 1603 500 394 63
PciI A'CATG_T
Cuts at: 3705 3705
Size: 4553
PflMI CCAn_nnn'nTGG
Cuts at: 969 969
Size: 4553
PmeI GTTT'AAAC
Cuts at: 1552 1552
Size: 4553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2999 3031 2999
Size: 32 4521
PshAI GACnn'nnGTC
Cuts at: 1183 1183
Size: 4553
PspOMI G'GGCC_C
Cuts at: 703 703
Size: 4553
PstI C_TGCA'G
Cuts at: 1053 1600 2285 1053
Size: 547 685 3321
Fragments arranged by size:
3321 685 547
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4553
PvuII CAG'CTG
Cuts at: 798 1978 2338 798
Size: 1180 360 3013
Fragments arranged by size:
3013 1180 360
RsrII CG'GwC_CG
Cuts at: 2748 2748
Size: 4553
SacI G_AGCT'C
Cuts at: 1567 1567
Size: 4553
SalI G'TCGA_C
Cuts at: 1064 1590 1064
Size: 526 4027
SapI GCTCTTCn'nnn_
Cuts at: 2576 2786 2576
Size: 210 4343
SbfI CC_TGCA'GG
Cuts at: 1600 1600
Size: 4553
ScaI AGT'ACT
Cuts at: 4070 4070
Size: 4553
SexAI A'CCwGG_T
Cuts at: 566 566
Size: 4553
SfcI C'TryA_G
Cuts at: 33 1049 1596 2281 3249 3440 33
Size: 1016 547 685 968 191 1146
Fragments arranged by size:
1146 1016 968 685 547 191
SfoI GGC'GCC
Cuts at: 285 1161 1332 1508 1985 2233 285
Size: 876 171 176 477 248 2605
Fragments arranged by size:
2605 876 477 248 176 171
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
SgrAI Cr'CCGG_yG
Cuts at: 295 295
Size: 4553
SmaI CCC'GGG
Cuts at: 365 756 1575 365
Size: 391 819 3343
Fragments arranged by size:
3343 819 391
SmlI C'TyrA_G
Cuts at: 1708 2623 3337 3599 1708
Size: 915 714 262 2662
Fragments arranged by size:
2662 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4553
SphI G_CATG'C
Cuts at: 1149 1606 2106 1149
Size: 457 500 3596
Fragments arranged by size:
3596 500 457
StyI C'CwwG_G
Cuts at: 107 468 1217 1358 1687 2041 107
Size: 361 749 141 329 354 2619
Fragments arranged by size:
2619 749 361 354 329 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 589 848 1013 2136 2269 2728 2937 589
Size: 259 165 1123 133 459 209 2205
Fragments arranged by size:
2205 1123 459 259 209 165 133
TatI w'GTAC_w
Cuts at: 1322 4068 1322
Size: 2746 1807
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1474 1506 1519 1551 4070 4102 1474
Size: 32 13 32 2519 32 1925
Fragments arranged by size:
2519 1925 32 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 2350 2350
Size: 4553
XbaI T'CTAG_A
Cuts at: 190 1584 190
Size: 1394 3159
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 938 2048 938
Size: 1110 3443
XmaI C'CCGG_G
Cuts at: 363 754 1573 363
Size: 391 819 3343
Fragments arranged by size:
3343 819 391
XmnI GAAnn'nnTTC
Cuts at: 622 622
Size: 4553
ZraI GAC'GTC
Cuts at: 198 198
Size: 4553
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PmeI PpiI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AflII AgeI AhdI AleI AlfI AloI AscI
BaeI BglI BmtI BsiWI BspEI BsrGI BstZ17I ClaI
CspCI DraIII EcoRV FseI HpaI MfeI NdeI NheI
NotI NruI PacI PasI PfoI PmlI PpuMI PsiI
PspXI PsrI SacII SanDI SfiI SnaBI SrfI SspI
StuI SwaI XhoI