Restriction map for
pF1KB4800
(Circular) (Six-base) MAPSORT of: pF1KB4800.seq Check: 8452 from: 1 to: 4379
pF1KB4800 4379 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1106 1106
Size: 4379
AccI GT'mk_AC
Cuts at: 1417 1417
Size: 4379
Acc65I G'GTAC_C
Cuts at: 946 1395 946
Size: 449 3930
AclI AA'CG_TT
Cuts at: 4219 4219
Size: 4379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2204 2636 2989 3651 2204
Size: 432 353 662 2932
Fragments arranged by size:
2932 662 432 353
AflIII A'CryG_T
Cuts at: 1731 3531 3812 1731
Size: 1800 281 2298
Fragments arranged by size:
2298 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 270 717 270
Size: 447 3932
AleI CACnn'nnGTG
Cuts at: 1196 1196
Size: 4379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 865 865
Size: 4379
AlwNI CAG_nnn'CTG
Cuts at: 344 1684 3122 344
Size: 1340 1438 1601
Fragments arranged by size:
1601 1438 1340
ApaI G_GGCC'C
Cuts at: 540 540
Size: 4379
ApaLI G'TGCA_C
Cuts at: 840 922 1021 3217 840
Size: 82 99 2196 2002
Fragments arranged by size:
2196 2002 99 82
ApoI r'AATT_y
Cuts at: 1383 3732 3988 1383
Size: 2349 256 1774
Fragments arranged by size:
2349 1774 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4379
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
AvaI C'yCGr_G
Cuts at: 260 775 916 1399 260
Size: 515 141 483 3240
Fragments arranged by size:
3240 515 483 141
AvrII C'CTAG_G
Cuts at: 696 696
Size: 4379
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 930 963 930
Size: 33 4346
BamHI G'GATC_C
Cuts at: 1404 1404
Size: 4379
BanI G'GyrC_C
Cuts at: 207 506 648 676 946 1346 1395 1809 2057
Size: 299 142 28 270 400 49 414 248
Cuts at: 2057 2092 207
Size: 35 2494
Fragments arranged by size:
2494 414 400 299 270 248 142 49
35 28
BanII G_rGCy'C
Cuts at: 260 358 540 704 917 1249 1370 1393 2423
Size: 98 182 164 213 332 121 23 1030
Cuts at: 2423 260
Size: 2216
Fragments arranged by size:
2216 1030 332 213 182 164 121 98
23
BbeI G_GCGC'C
Cuts at: 211 652 1813 2061 211
Size: 441 1161 248 2529
Fragments arranged by size:
2529 1161 441 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 966 1000 90
Size: 34 842 34 3469
Fragments arranged by size:
3469 842 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2277 3333 4379 2277
Size: 1056 1046 2277
Fragments arranged by size:
2277 1056 1046
BclI T'GATC_A
Cuts at: 1899 1899
Size: 4379
BglI GCCn_nnn'nGGC
Cuts at: 912 912
Size: 4379
BglII A'GATC_T
Cuts at: 1052 1894 3931 1052
Size: 842 2037 1500
Fragments arranged by size:
2037 1500 842
BlpI GC'TnA_GC
Cuts at: 1491 1491
Size: 4379
Bme1580I G_kGCm'C
Cuts at: 427 540 844 926 992 1025 2004 2097 3221
Size: 113 304 82 66 33 979 93 1124
Cuts at: 3221 427
Size: 1585
Fragments arranged by size:
1585 1124 979 304 113 93 82 66
33
BmrI ACTGGGnnnn_n'
Cuts at: 1591 1672 2007 4151 1591
Size: 81 335 2144 1819
Fragments arranged by size:
2144 1819 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 4379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 396 562 1024 386
Size: 10 166 462 3741
Fragments arranged by size:
3741 462 166 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1555 2434 3148 3446 1555
Size: 879 714 298 2488
Fragments arranged by size:
2488 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4083 4083
Size: 4379
BsaAI yAC'GTr
Cuts at: 927 1732 927
Size: 805 3574
BsaBI GATnn'nnATC
Cuts at: 6 1893 3930 6
Size: 1887 2037 455
Fragments arranged by size:
2037 1887 455
BsaHI Gr'CG_yC
Cuts at: 208 649 815 1810 2058 2760 4256 208
Size: 441 166 995 248 702 1496 331
Fragments arranged by size:
1496 995 702 441 331 248 166
BsaWI w'CCGG_w
Cuts at: 1568 2089 2767 3178 3325 3599 1568
Size: 521 678 411 147 274 2348
Fragments arranged by size:
2348 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 295 325 823 853 295
Size: 30 498 30 3821
Fragments arranged by size:
3821 498 30 30
BseYI C'CCAG_C
Cuts at: 443 855 1804 2562 3227 3635 443
Size: 412 949 758 665 408 1187
Fragments arranged by size:
1187 949 758 665 412 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 778 906 535
Size: 243 128 4008
Fragments arranged by size:
4008 243 128
BsiEI CG_ry'CG
Cuts at: 104 529 602 1921 1967 3197 104
Size: 425 73 1319 46 1230 1286
Fragments arranged by size:
1319 1286 1230 425 73 46
BsiHKAI G_wGCw'C
Cuts at: 358 844 926 1025 1249 1393 2171 2361 3221
Size: 486 82 99 224 144 778 190 860
Cuts at: 3221 358
Size: 1516
Fragments arranged by size:
1516 860 778 486 224 190 144 99
82
BsmI GAATG_Cn'
Cuts at: 1234 1234
Size: 4379
BsmBI CGTCTCn'nnnn_
Cuts at: 404 1348 404
Size: 944 3435
Bsp1286I G_dGCh'C
Cuts at: 260 358 427 540 704 844 917 926 992
Size: 98 69 113 164 140 73 9 66
Cuts at: 992 1025 1249 1370 1393 2004 2097 2171 2361
Size: 33 224 121 23 611 93 74 190
Cuts at: 2361 2423 3221 260
Size: 62 798 1418
Fragments arranged by size:
1418 798 611 224 190 164 140 121
113 98 93 74 73 69 66 62
33 23 9
BspHI T'CATG_A
Cuts at: 2811 2811
Size: 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 509 923 1106 1429 1643 1945 2326 509
Size: 414 183 323 214 302 381 2562
Fragments arranged by size:
2562 414 381 323 302 214 183
BsrBI CCG'CTC
Cuts at: 50 482 2671 2725 4209 4374 50
Size: 432 2189 54 1484 165 55
Fragments arranged by size:
2189 1484 432 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2291 2291
Size: 4379
BsrFI r'CCGG_y
Cuts at: 204 628 673 2377 2558 204
Size: 424 45 1704 181 2025
Fragments arranged by size:
2025 1704 424 181 45
BssSI C'ACGA_G
Cuts at: 2650 3358 2650
Size: 708 3671
BstAPI GCAn_nnn'nTGC
Cuts at: 344 513 884 1756 344
Size: 169 371 872 2967
Fragments arranged by size:
2967 872 371 169
BstBI TT'CG_AA
Cuts at: 2740 2740
Size: 4379
BstYI r'GATC_y
Cuts at: 1 1052 1404 1871 1894 2229 2475 2781 2793
Size: 1051 352 467 23 335 246 306 12
Cuts at: 2793 2879 3931 1
Size: 86 1052 449
Fragments arranged by size:
1052 1051 467 449 352 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 749 786 749
Size: 37 4342
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 413 2329 2510 2673 413
Size: 1916 181 163 2119
Fragments arranged by size:
2119 1916 181 163
BtsI GCAGTG_nn'
Cuts at: 1622 1622
Size: 4379
DraI TTT'AAA
Cuts at: 1378 1378
Size: 4379
DraIII CAC_nnn'GTG
Cuts at: 513 996 513
Size: 483 3896
DrdI GACnn_nn'nnGTC
Cuts at: 2085 3429 2085
Size: 1344 3035
EaeI y'GGCC_r
Cuts at: 591 599 783 1964 2138 2529 2556 3901 591
Size: 8 184 1181 174 391 27 1345 1069
Fragments arranged by size:
1345 1181 1069 391 184 174 27 8
EagI C'GGCC_G
Cuts at: 599 1964 599
Size: 1365 3014
EarI CTCTTCn'nnn_
Cuts at: 571 1057 2402 2612 571
Size: 486 1345 210 2338
Fragments arranged by size:
2338 1345 486 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 262 268 2587 3329 3475 4189 262
Size: 6 2319 742 146 714 452
Fragments arranged by size:
2319 742 714 452 146 6
EcoICRI GAG'CTC
Cuts at: 356 1247 1391 356
Size: 891 144 3344
Fragments arranged by size:
3344 891 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 396 562 1024 2204 2636 2989 3651 386
Size: 10 166 462 1180 432 353 662 1114
Fragments arranged by size:
1180 1114 662 462 432 353 166 10
EcoNI CCTnn'n_nnAGG
Cuts at: 695 695
Size: 4379
EcoO109I rG'GnC_Cy
Cuts at: 380 536 537 935 1518 380
Size: 156 1 398 583 3241
Fragments arranged by size:
3241 583 398 156 1
EcoRI G'AATT_C
Cuts at: 1383 1383
Size: 4379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4379
FspI TGC'GCA
Cuts at: 931 2160 931
Size: 1229 3150
HaeII r_GCGC'y
Cuts at: 211 554 652 812 1813 2061 3291 4062 211
Size: 343 98 160 1001 248 1230 771 528
Fragments arranged by size:
1230 1001 771 528 343 248 160 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 256 288 295 327 703 735 2221 2251 2253
Size: 32 7 32 376 32 1486 30 2
Cuts at: 2253 2283 256
Size: 30 2352
Fragments arranged by size:
2352 1486 376 32 32 32 30 30
7 2
HincII GTy'rAC
Cuts at: 1418 1418
Size: 4379
KasI G'GCGC_C
Cuts at: 207 648 1809 2057 207
Size: 441 1161 248 2529
Fragments arranged by size:
2529 1161 441 248
KpnI G_GTAC'C
Cuts at: 950 1399 950
Size: 449 3930
MluI A'CGCG_T
Cuts at: 3812 3812
Size: 4379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 809 1274 3138 3322 809
Size: 465 1864 184 1866
Fragments arranged by size:
1866 1864 465 184
MscI TGG'CCA
Cuts at: 593 785 2140 593
Size: 192 1355 2832
Fragments arranged by size:
2832 1355 192
MslI CAynn'nnrTG
Cuts at: 1196 2368 2495 1196
Size: 1172 127 3080
Fragments arranged by size:
3080 1172 127
MspA1I CmG'CkG
Cuts at: 288 329 685 757 944 1490 1804 2164 2948
Size: 41 356 72 187 546 314 360 784
Cuts at: 2948 3193 288
Size: 245 1474
Fragments arranged by size:
1474 784 546 360 356 314 245 187
72 41
NaeI GCC'GGC
Cuts at: 630 2560 630
Size: 1930 2449
NarI GG'CG_CC
Cuts at: 208 649 1810 2058 208
Size: 441 1161 248 2529
Fragments arranged by size:
2529 1161 441 248
NgoMIV G'CCGG_C
Cuts at: 628 2558 628
Size: 1930 2449
NspI r_CATG'y
Cuts at: 1432 1932 3535 1432
Size: 500 1603 2276
Fragments arranged by size:
2276 1603 500
PciI A'CATG_T
Cuts at: 3531 3531
Size: 4379
PmeI GTTT'AAAC
Cuts at: 1378 1378
Size: 4379
PmlI CAC'GTG
Cuts at: 927 927
Size: 4379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2857 2825
Size: 32 4347
PpuMI rG'GwC_Cy
Cuts at: 935 935
Size: 4379
PspOMI G'GGCC_C
Cuts at: 536 536
Size: 4379
PspXI vC'TCGA_Gb
Cuts at: 916 916
Size: 4379
PstI C_TGCA'G
Cuts at: 664 1426 2111 664
Size: 762 685 2932
Fragments arranged by size:
2932 762 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4379
PvuII CAG'CTG
Cuts at: 288 329 1804 2164 288
Size: 41 1475 360 2503
Fragments arranged by size:
2503 1475 360 41
RsrII CG'GwC_CG
Cuts at: 2574 2574
Size: 4379
SacI G_AGCT'C
Cuts at: 358 1249 1393 358
Size: 891 144 3344
Fragments arranged by size:
3344 891 144
SalI G'TCGA_C
Cuts at: 1416 1416
Size: 4379
SapI GCTCTTCn'nnn_
Cuts at: 1057 2402 2612 1057
Size: 1345 210 2824
Fragments arranged by size:
2824 1345 210
SbfI CC_TGCA'GG
Cuts at: 1426 1426
Size: 4379
ScaI AGT'ACT
Cuts at: 1138 3896 1138
Size: 2758 1621
SexAI A'CCwGG_T
Cuts at: 509 509
Size: 4379
SfcI C'TryA_G
Cuts at: 33 660 1422 2107 3075 3266 33
Size: 627 762 685 968 191 1146
Fragments arranged by size:
1146 968 762 685 627 191
SfoI GGC'GCC
Cuts at: 209 650 1811 2059 209
Size: 441 1161 248 2529
Fragments arranged by size:
2529 1161 441 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
SmaI CCC'GGG
Cuts at: 262 1401 262
Size: 1139 3240
SmlI C'TyrA_G
Cuts at: 916 1534 2449 3163 3425 916
Size: 618 915 714 262 1870
Fragments arranged by size:
1870 915 714 618 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4379
SphI G_CATG'C
Cuts at: 1432 1932 1432
Size: 500 3879
StyI C'CwwG_G
Cuts at: 696 1513 1867 696
Size: 817 354 3208
Fragments arranged by size:
3208 817 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 307 1276 1962 2095 2554 2763 307
Size: 969 686 133 459 209 1923
Fragments arranged by size:
1923 969 686 459 209 133
TatI w'GTAC_w
Cuts at: 1136 3894 1136
Size: 2758 1621
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 823 855 3896 3928 823
Size: 32 3041 32 1274
Fragments arranged by size:
3041 1274 32 32
Tth111I GACn'n_nGTC
Cuts at: 2176 2176
Size: 4379
XbaI T'CTAG_A
Cuts at: 1410 1410
Size: 4379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1874 1874
Size: 4379
XhoI C'TCGA_G
Cuts at: 916 916
Size: 4379
XmaI C'CCGG_G
Cuts at: 260 1399 260
Size: 1139 3240
XmnI GAAnn'nnTTC
Cuts at: 1066 1318 1066
Size: 252 4127
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AloI AscI BbsI BbvCI
BfrBI BmgBI BmtI Bpu10I BseRI BsiWI BspEI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PshAI
PsiI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI ZraI