Restriction map for
pF1KB4774
(Circular) (Six-base) MAPSORT of: pF1KB4774.seq Check: 3153 from: 1 to: 5303
pF1KB4774 5303 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 478 478
Size: 5303
AccI GT'mk_AC
Cuts at: 1397 2341 1397
Size: 944 4359
Acc65I G'GTAC_C
Cuts at: 2319 2319
Size: 5303
AclI AA'CG_TT
Cuts at: 5143 5143
Size: 5303
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 3128 3560 3913 4575 877
Size: 2251 432 353 662 1605
Fragments arranged by size:
2251 1605 662 432 353
AflIII A'CryG_T
Cuts at: 2655 4455 4736 2655
Size: 1800 281 3222
Fragments arranged by size:
3222 1800 281
AleI CACnn'nnGTG
Cuts at: 593 593
Size: 5303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2014 2014
Size: 5303
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 922 954 1186 1218 922
Size: 32 232 32 5007
Fragments arranged by size:
5007 232 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2608 4046 2608
Size: 1438 3865
ApaI G_GGCC'C
Cuts at: 303 694 303
Size: 391 4912
ApaLI G'TGCA_C
Cuts at: 4141 4141
Size: 5303
ApoI r'AATT_y
Cuts at: 1351 2263 2307 4656 4912 1351
Size: 912 44 2349 256 1742
Fragments arranged by size:
2349 1742 912 256 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 5303
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5303
AvaI C'yCGr_G
Cuts at: 139 1174 1834 1920 2119 2240 2323 139
Size: 1035 660 86 199 121 83 3119
Fragments arranged by size:
3119 1035 660 199 121 86 83
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 950 983 950
Size: 33 5270
BamHI G'GATC_C
Cuts at: 1499 2328 1499
Size: 829 4474
BanI G'GyrC_C
Cuts at: 1275 1793 2319 2733 2981 3016 1275
Size: 518 526 414 248 35 3562
Fragments arranged by size:
3562 526 518 414 248 35
BanII G_rGCy'C
Cuts at: 227 303 694 2317 3347 227
Size: 76 391 1623 1030 2183
Fragments arranged by size:
2183 1623 1030 391 76
BbeI G_GCGC'C
Cuts at: 1279 2737 2985 1279
Size: 1458 248 3597
Fragments arranged by size:
3597 1458 248
BbsI GAAGACnn'nnnn_
Cuts at: 549 549
Size: 5303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 325 359 907 941 1552 1586 1605
Size: 34 201 34 548 34 611 34 19
Cuts at: 1605 1639 2221 2255 90
Size: 34 582 34 3138
Fragments arranged by size:
3138 611 582 548 201 34 34 34
34 34 34 19
BciVI GTATCCnnnnn_n'
Cuts at: 951 3201 4257 5303 951
Size: 2250 1056 1046 951
Fragments arranged by size:
2250 1056 1046 951
BclI T'GATC_A
Cuts at: 2823 2823
Size: 5303
BfrBI ATG'CAT
Cuts at: 918 918
Size: 5303
BglII A'GATC_T
Cuts at: 2818 4855 2818
Size: 2037 3266
BlpI GC'TnA_GC
Cuts at: 1021 2415 1021
Size: 1394 3909
Bme1580I G_kGCm'C
Cuts at: 303 694 1066 1604 2928 3021 4145 303
Size: 391 372 538 1324 93 1124 1461
Fragments arranged by size:
1461 1324 1124 538 391 372 93
BmgBI CAC'GTC
Cuts at: 759 1202 1670 759
Size: 443 468 4392
Fragments arranged by size:
4392 468 443
BmrI ACTGGGnnnn_n'
Cuts at: 588 1977 2130 2515 2596 2931 5075 588
Size: 1389 153 385 81 335 2144 816
Fragments arranged by size:
2144 1389 816 385 335 153 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 234 2243 3670 234
Size: 2009 1427 1867
Fragments arranged by size:
2009 1867 1427
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 628 924 1287 1606 289
Size: 339 296 363 319 3986
Fragments arranged by size:
3986 363 339 319 296
Bpu10I CC'TnA_GC
Cuts at: 1313 1313
Size: 5303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1672 2479 3358 4072 4370 1672
Size: 807 879 714 298 2605
Fragments arranged by size:
2605 879 807 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 886 1072 5007 886
Size: 186 3935 1182
Fragments arranged by size:
3935 1182 186
BsaAI yAC'GTr
Cuts at: 672 1524 2656 672
Size: 852 1132 3319
Fragments arranged by size:
3319 1132 852
BsaBI GATnn'nnATC
Cuts at: 6 2817 4854 6
Size: 2811 2037 455
Fragments arranged by size:
2811 2037 455
BsaHI Gr'CG_yC
Cuts at: 475 1276 2247 2734 2982 3684 5180 475
Size: 801 971 487 248 702 1496 598
Fragments arranged by size:
1496 971 801 702 598 487 248
BsaWI w'CCGG_w
Cuts at: 2285 2492 3013 3691 4102 4249 4523 2285
Size: 207 521 678 411 147 274 3065
Fragments arranged by size:
3065 678 521 411 274 207 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 865 895 922 952 1093 1123 1978 2008 2149
Size: 30 27 30 141 30 855 30 141
Cuts at: 2149 2179 865
Size: 30 3989
Fragments arranged by size:
3989 855 141 141 30 30 30 30
30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 1098 223
Size: 875 4428
BseYI C'CCAG_C
Cuts at: 391 750 1065 1477 2728 3486 4151 4559 391
Size: 359 315 412 1251 758 665 408 1135
Fragments arranged by size:
1251 1135 758 665 412 408 359 315
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1842 2293 1842
Size: 451 4852
BsiEI CG_ry'CG
Cuts at: 104 168 172 2845 2891 4121 104
Size: 64 4 2673 46 1230 1286
Fragments arranged by size:
2673 1286 1230 64 46 4
BsiHKAI G_wGCw'C
Cuts at: 406 1669 1762 2317 3095 3285 4145 406
Size: 1263 93 555 778 190 860 1564
Fragments arranged by size:
1564 1263 860 778 555 190 93
BsmBI CGTCTCn'nnnn_
Cuts at: 1329 1701 1329
Size: 372 4931
Bsp1286I G_dGCh'C
Cuts at: 227 303 406 694 1066 1604 1669 1762 2317
Size: 76 103 288 372 538 65 93 555
Cuts at: 2317 2928 3021 3095 3285 3347 4145 227
Size: 611 93 74 190 62 798 1385
Fragments arranged by size:
1385 798 611 555 538 372 288 190
103 93 93 76 74 65 62
BspHI T'CATG_A
Cuts at: 3735 3735
Size: 5303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2353 2567 2869 3250 2353
Size: 214 302 381 4406
Fragments arranged by size:
4406 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3595 3649 5133 5298 50
Size: 3545 54 1484 165 55
Fragments arranged by size:
3545 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 272 1034 2034 3215 272
Size: 762 1000 1181 2360
Fragments arranged by size:
2360 1181 1000 762
BsrFI r'CCGG_y
Cuts at: 167 461 1472 3301 3482 167
Size: 294 1011 1829 181 1988
Fragments arranged by size:
1988 1829 1011 294 181
BssSI C'ACGA_G
Cuts at: 745 3574 4282 745
Size: 2829 708 1766
Fragments arranged by size:
2829 1766 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1487 2680 1487
Size: 1193 4110
BstBI TT'CG_AA
Cuts at: 3664 3664
Size: 5303
BstYI r'GATC_y
Cuts at: 1 324 451 1499 2328 2795 2818 3153 3399
Size: 323 127 1048 829 467 23 335 246
Cuts at: 3399 3705 3717 3803 4855 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 1048 829 467 449 335 323 306
246 127 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 295 869 295
Size: 574 4729
BtgI C'CryG_G
Cuts at: 681 1773 681
Size: 1092 4211
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3253 3434 3597 3253
Size: 181 163 4959
Fragments arranged by size:
4959 181 163
BtsI GCAGTG_nn'
Cuts at: 1146 2070 2546 1146
Size: 924 476 3903
Fragments arranged by size:
3903 924 476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2085 2120 2085
Size: 35 5268
DraI TTT'AAA
Cuts at: 2302 2302
Size: 5303
DraIII CAC_nnn'GTG
Cuts at: 1152 1152
Size: 5303
DrdI GACnn_nn'nnGTC
Cuts at: 3009 4353 3009
Size: 1344 3959
EaeI y'GGCC_r
Cuts at: 165 169 182 787 1005 1437 2888 3062 3453
Size: 4 13 605 218 432 1451 174 391
Cuts at: 3453 3480 4825 165
Size: 27 1345 643
Fragments arranged by size:
1451 1345 643 605 432 391 218 174
27 13 4
EagI C'GGCC_G
Cuts at: 165 169 2888 165
Size: 4 2719 2580
Fragments arranged by size:
2719 2580 4
EarI CTCTTCn'nnn_
Cuts at: 281 3326 3536 281
Size: 3045 210 2048
Fragments arranged by size:
3045 2048 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 220 1512 1898 3511 4253 4399 5113 220
Size: 1292 386 1613 742 146 714 410
Fragments arranged by size:
1613 1292 742 714 410 386 146
EcoICRI GAG'CTC
Cuts at: 2315 2315
Size: 5303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 628 877 924 1287 1606 3128 3560 3913
Size: 339 249 47 363 319 1522 432 353
Cuts at: 3913 4575 289
Size: 662 1017
Fragments arranged by size:
1522 1017 662 432 363 353 339 319
249 47
EcoNI CCTnn'n_nnAGG
Cuts at: 205 205
Size: 5303
EcoO109I rG'GnC_Cy
Cuts at: 291 299 300 690 691 2442 291
Size: 8 1 390 1 1751 3152
Fragments arranged by size:
3152 1751 390 8 1 1
EcoRI G'AATT_C
Cuts at: 1351 2307 1351
Size: 956 4347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1404 2064 2220 3322 1404
Size: 660 156 1102 3385
Fragments arranged by size:
3385 1102 660 156
FseI GG_CCGG'CC
Cuts at: 171 171
Size: 5303
FspI TGC'GCA
Cuts at: 2062 3084 2062
Size: 1022 4281
HaeII r_GCGC'y
Cuts at: 1279 1560 1632 1863 2737 2985 4215 4986 1279
Size: 281 72 231 874 248 1230 771 1596
Fragments arranged by size:
1596 1230 874 771 281 248 231 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 852 865 884 897 1744 1776 2149 2181 3145
Size: 13 19 13 847 32 373 32 964
Cuts at: 3145 3175 3177 3207 852
Size: 30 2 30 2948
Fragments arranged by size:
2948 964 847 373 32 32 30 30
19 13 13 2
HincII GTy'rAC
Cuts at: 1734 2342 1734
Size: 608 4695
HindIII A'AGCT_T
Cuts at: 1051 1051
Size: 5303
KasI G'GCGC_C
Cuts at: 1275 2733 2981 1275
Size: 1458 248 3597
Fragments arranged by size:
3597 1458 248
KpnI G_GTAC'C
Cuts at: 2323 2323
Size: 5303
MfeI C'AATT_G
Cuts at: 1368 1368
Size: 5303
MluI A'CGCG_T
Cuts at: 4736 4736
Size: 5303
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1356 2192 4062 4246 1356
Size: 836 1870 184 2413
Fragments arranged by size:
2413 1870 836 184
MscI TGG'CCA
Cuts at: 1007 1439 3064 1007
Size: 432 1625 3246
Fragments arranged by size:
3246 1625 432
MslI CAynn'nnrTG
Cuts at: 593 2152 3292 3419 593
Size: 1559 1140 127 2477
Fragments arranged by size:
2477 1559 1140 127
MspA1I CmG'CkG
Cuts at: 132 174 395 683 1568 1775 1791 1826 1865
Size: 42 221 288 885 207 16 35 39
Cuts at: 1865 1968 2077 2414 2728 3088 3872 4117 132
Size: 103 109 337 314 360 784 245 1318
Fragments arranged by size:
1318 885 784 360 337 314 288 245
221 207 109 103 42 39 35 16
NaeI GCC'GGC
Cuts at: 169 463 3484 169
Size: 294 3021 1988
Fragments arranged by size:
3021 1988 294
NarI GG'CG_CC
Cuts at: 1276 2734 2982 1276
Size: 1458 248 3597
Fragments arranged by size:
3597 1458 248
NgoMIV G'CCGG_C
Cuts at: 167 461 3482 167
Size: 294 3021 1988
Fragments arranged by size:
3021 1988 294
NsiI A_TGCA'T
Cuts at: 920 920
Size: 5303
NspI r_CATG'y
Cuts at: 1146 2151 2356 2856 4459 1146
Size: 1005 205 500 1603 1990
Fragments arranged by size:
1990 1603 1005 500 205
PasI CC'CwG_GG
Cuts at: 885 1806 885
Size: 921 4382
PciI A'CATG_T
Cuts at: 4455 4455
Size: 5303
PflMI CCAn_nnn'nTGG
Cuts at: 750 1072 750
Size: 322 4981
PfoI T'CCnGG_A
Cuts at: 372 605 953 372
Size: 233 348 4722
Fragments arranged by size:
4722 348 233
PmeI GTTT'AAAC
Cuts at: 2302 2302
Size: 5303
PmlI CAC'GTG
Cuts at: 1524 1524
Size: 5303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 922 954 3749 3781 922
Size: 32 2795 32 2444
Fragments arranged by size:
2795 2444 32 32
PpuMI rG'GwC_Cy
Cuts at: 291 291
Size: 5303
PspOMI G'GGCC_C
Cuts at: 299 690 299
Size: 391 4912
PstI C_TGCA'G
Cuts at: 369 2003 2234 2350 3035 369
Size: 1634 231 116 685 2637
Fragments arranged by size:
2637 1634 685 231 116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5303
PvuII CAG'CTG
Cuts at: 395 1568 1826 1968 2077 2728 3088 395
Size: 1173 258 142 109 651 360 2610
Fragments arranged by size:
2610 1173 651 360 258 142 109
RsrII CG'GwC_CG
Cuts at: 3498 3498
Size: 5303
SacI G_AGCT'C
Cuts at: 2317 2317
Size: 5303
SacII CC_GC'GG
Cuts at: 684 1776 684
Size: 1092 4211
SalI G'TCGA_C
Cuts at: 2340 2340
Size: 5303
SapI GCTCTTCn'nnn_
Cuts at: 3326 3536 3326
Size: 210 5093
SbfI CC_TGCA'GG
Cuts at: 2350 2350
Size: 5303
ScaI AGT'ACT
Cuts at: 4820 4820
Size: 5303
SfcI C'TryA_G
Cuts at: 33 365 1999 2230 2346 3031 3999 4190 33
Size: 332 1634 231 116 685 968 191 1146
Fragments arranged by size:
1634 1146 968 685 332 231 191 116
SfoI GGC'GCC
Cuts at: 1277 2735 2983 1277
Size: 1458 248 3597
Fragments arranged by size:
3597 1458 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5303
SmaI CCC'GGG
Cuts at: 1176 1836 1922 2325 1176
Size: 660 86 403 4154
Fragments arranged by size:
4154 660 403 86
SmlI C'TyrA_G
Cuts at: 1687 2458 3373 4087 4349 1687
Size: 771 915 714 262 2641
Fragments arranged by size:
2641 915 771 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5303
SphI G_CATG'C
Cuts at: 2356 2856 2356
Size: 500 4803
SrfI GCCC'GGGC
Cuts at: 1922 1922
Size: 5303
StyI C'CwwG_G
Cuts at: 1931 2437 2791 1931
Size: 506 354 4443
Fragments arranged by size:
4443 506 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1996 2886 3019 3478 3687 1996
Size: 890 133 459 209 3612
Fragments arranged by size:
3612 890 459 209 133
TatI w'GTAC_w
Cuts at: 2138 4818 2138
Size: 2680 2623
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4820 4852 4820
Size: 32 5271
Tth111I GACn'n_nGTC
Cuts at: 3100 3100
Size: 5303
XbaI T'CTAG_A
Cuts at: 2334 2334
Size: 5303
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 491 2798 114
Size: 377 2307 2619
Fragments arranged by size:
2619 2307 377
XmaI C'CCGG_G
Cuts at: 1174 1834 1920 2323 1174
Size: 660 86 403 4154
Fragments arranged by size:
4154 660 403 86
XmnI GAAnn'nnTTC
Cuts at: 381 934 381
Size: 553 4750
ZraI GAC'GTC
Cuts at: 476 476
Size: 5303
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AscI AvrII BbvCI
BglI BmtI BsiWI BsmI BspEI BsrGI BssHII BstEII
BstXI BstZ17I ClaI EcoRV FspAI HpaI NcoI NdeI
NheI NotI NruI PacI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SspI StuI SwaI
XhoI