Restriction map for
pF1KB4714
(Circular) (Six-base) MAPSORT of: pF1KB4714.seq Check: 7674 from: 1 to: 5735
pF1KB4714 5735 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 611 1564 2661 611
Size: 953 1097 3685
Fragments arranged by size:
3685 1097 953
AccI GT'mk_AC
Cuts at: 2482 2705 2773 2482
Size: 223 68 5444
Fragments arranged by size:
5444 223 68
Acc65I G'GTAC_C
Cuts at: 2751 2751
Size: 5735
AclI AA'CG_TT
Cuts at: 2470 5575 2470
Size: 3105 2630
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1666 3560 3992 4345 5007 628
Size: 1038 1894 432 353 662 1356
Fragments arranged by size:
1894 1356 1038 662 432 353
AfeI AGC'GCT
Cuts at: 532 634 532
Size: 102 5633
AflII C'TTAA_G
Cuts at: 775 1153 775
Size: 378 5357
AflIII A'CryG_T
Cuts at: 2585 3087 4887 5168 2585
Size: 502 1800 281 3152
Fragments arranged by size:
3152 1800 502 281
AhdI GACnn_n'nnGTC
Cuts at: 1836 1836
Size: 5735
AleI CACnn'nnGTG
Cuts at: 1422 2233 2263 2543 1422
Size: 811 30 280 4614
Fragments arranged by size:
4614 811 280 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 532 532
Size: 5735
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1432 1464 2455 2487 1432
Size: 32 991 32 4680
Fragments arranged by size:
4680 991 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1579 3040 4478 1579
Size: 1461 1438 2836
Fragments arranged by size:
2836 1461 1438
ApaI G_GGCC'C
Cuts at: 475 1499 1991 475
Size: 1024 492 4219
Fragments arranged by size:
4219 1024 492
ApaLI G'TGCA_C
Cuts at: 4573 4573
Size: 5735
ApoI r'AATT_y
Cuts at: 118 1228 2452 2739 5088 5344 118
Size: 1110 1224 287 2349 256 509
Fragments arranged by size:
2349 1224 1110 509 287 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5735
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5735
AvaI C'yCGr_G
Cuts at: 2215 2755 2215
Size: 540 5195
BamHI G'GATC_C
Cuts at: 2760 2760
Size: 5735
BanI G'GyrC_C
Cuts at: 561 1857 2751 3165 3413 3448 561
Size: 1296 894 414 248 35 2848
Fragments arranged by size:
2848 1296 894 414 248 35
BanII G_rGCy'C
Cuts at: 475 489 704 1499 1608 1791 1991 2015 2156
Size: 14 215 795 109 183 200 24 141
Cuts at: 2156 2749 3779 475
Size: 593 1030 2431
Fragments arranged by size:
2431 1030 795 593 215 200 183 141
109 24 14
BbeI G_GCGC'C
Cuts at: 1861 3169 3417 1861
Size: 1308 248 4179
Fragments arranged by size:
4179 1308 248
BbsI GAAGACnn'nnnn_
Cuts at: 464 584 2258 464
Size: 120 1674 3941
Fragments arranged by size:
3941 1674 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 473 507 473
Size: 34 5701
BciVI GTATCCnnnnn_n'
Cuts at: 3633 4689 5735 3633
Size: 1056 1046 3633
Fragments arranged by size:
3633 1056 1046
BclI T'GATC_A
Cuts at: 749 2391 3255 749
Size: 1642 864 3229
Fragments arranged by size:
3229 1642 864
BglI GCCn_nnn'nGGC
Cuts at: 922 1135 922
Size: 213 5522
BglII A'GATC_T
Cuts at: 1196 1940 2370 3250 5287 1196
Size: 744 430 880 2037 1644
Fragments arranged by size:
2037 1644 880 744 430
BlpI GC'TnA_GC
Cuts at: 2847 2847
Size: 5735
Bme1580I G_kGCm'C
Cuts at: 243 475 566 1263 1499 1847 1991 3360 3453
Size: 232 91 697 236 348 144 1369 93
Cuts at: 3453 4577 243
Size: 1124 1401
Fragments arranged by size:
1401 1369 1124 697 348 236 232 144
93 91
BmgBI CAC'GTC
Cuts at: 624 624
Size: 5735
BmrI ACTGGGnnnn_n'
Cuts at: 458 560 1622 2333 2947 3028 3363 5507 458
Size: 102 1062 711 614 81 335 2144 686
Fragments arranged by size:
2144 1062 711 686 614 335 102 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4102 4102
Size: 5735
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 558 967 1114 1732 1801 175
Size: 383 409 147 618 69 4109
Fragments arranged by size:
4109 618 409 383 147 69
Bpu10I CC'TnA_GC
Cuts at: 650 2009 650
Size: 1359 4376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 1819 2911 3790 4504 4802 193
Size: 1626 1092 879 714 298 1126
Fragments arranged by size:
1626 1126 1092 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5439 5439
Size: 5735
BsaAI yAC'GTr
Cuts at: 2184 3088 2184
Size: 904 4831
BsaBI GATnn'nnATC
Cuts at: 6 3249 5286 6
Size: 3243 2037 455
Fragments arranged by size:
3243 2037 455
BsaHI Gr'CG_yC
Cuts at: 1858 3166 3414 4116 5612 1858
Size: 1308 248 702 1496 1981
Fragments arranged by size:
1981 1496 1308 702 248
BsaWI w'CCGG_w
Cuts at: 2924 3445 4123 4534 4681 4955 2924
Size: 521 678 411 147 274 3704
Fragments arranged by size:
3704 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 686 716 2455 2485 686
Size: 30 1739 30 3936
Fragments arranged by size:
3936 1739 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 847 1191 1576 2707 151
Size: 696 344 385 1131 3179
Fragments arranged by size:
3179 1131 696 385 344
BseYI C'CCAG_C
Cuts at: 635 3160 3918 4583 4991 635
Size: 2525 758 665 408 1379
Fragments arranged by size:
2525 1379 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 633 2040 2085 2164 2256 633
Size: 1407 45 79 92 4112
Fragments arranged by size:
4112 1407 92 79 45
BsiEI CG_ry'CG
Cuts at: 104 308 479 1182 3277 3323 4553 104
Size: 204 171 703 2095 46 1230 1286
Fragments arranged by size:
2095 1286 1230 703 204 171 46
BsiHKAI G_wGCw'C
Cuts at: 287 983 1590 2177 2749 3527 3717 4577 287
Size: 696 607 587 572 778 190 860 1445
Fragments arranged by size:
1445 860 778 696 607 587 572 190
BsmI GAATG_Cn'
Cuts at: 2540 2540
Size: 5735
BsmBI CGTCTCn'nnnn_
Cuts at: 2029 2029
Size: 5735
Bsp1286I G_dGCh'C
Cuts at: 243 287 475 489 566 704 983 1263 1499
Size: 44 188 14 77 138 279 280 236
Cuts at: 1499 1590 1608 1791 1847 1991 2015 2156 2177
Size: 91 18 183 56 144 24 141 21
Cuts at: 2177 2749 3360 3453 3527 3717 3779 4577 243
Size: 572 611 93 74 190 62 798 1401
Fragments arranged by size:
1401 798 611 572 280 279 236 190
188 183 144 141 138 93 91 77
74 62 56 44 24 21 18 14
BspHI T'CATG_A
Cuts at: 1187 2408 2619 4167 1187
Size: 1221 211 1548 2755
Fragments arranged by size:
2755 1548 1221 211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 611 1564 2230 2661 2785 2999 3301 3682 611
Size: 953 666 431 124 214 302 381 2664
Fragments arranged by size:
2664 953 666 431 381 302 214 124
BsrBI CCG'CTC
Cuts at: 50 4027 4081 5565 5730 50
Size: 3977 54 1484 165 55
Fragments arranged by size:
3977 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 398 1124 3647 398
Size: 726 2523 2486
Fragments arranged by size:
2523 2486 726
BsrFI r'CCGG_y
Cuts at: 3733 3914 3733
Size: 181 5554
BssHII G'CGCG_C
Cuts at: 1350 1350
Size: 5735
BssSI C'ACGA_G
Cuts at: 586 895 4006 4714 586
Size: 309 3111 708 1607
Fragments arranged by size:
3111 1607 708 309
BstAPI GCAn_nnn'nTGC
Cuts at: 3112 3112
Size: 5735
BstBI TT'CG_AA
Cuts at: 2423 4096 2423
Size: 1673 4062
BstXI CCAn_nnnn'nTGG
Cuts at: 960 1635 2082 2265 2688 960
Size: 675 447 183 423 4007
Fragments arranged by size:
4007 675 447 423 183
BstYI r'GATC_y
Cuts at: 1 1089 1196 1940 2370 2760 3227 3250 3585
Size: 1088 107 744 430 390 467 23 335
Cuts at: 3585 3831 4137 4149 4235 5287 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1088 1052 744 467 449 430 390 335
306 246 107 86 23 12
BtgI C'CryG_G
Cuts at: 296 1853 1862 1977 2015 296
Size: 1557 9 115 38 4016
Fragments arranged by size:
4016 1557 115 38 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3685 3866 4029 3685
Size: 181 163 5391
Fragments arranged by size:
5391 181 163
BtsI GCAGTG_nn'
Cuts at: 954 1970 2239 2978 954
Size: 1016 269 739 3711
Fragments arranged by size:
3711 1016 739 269
DraI TTT'AAA
Cuts at: 2734 2734
Size: 5735
DrdI GACnn_nn'nnGTC
Cuts at: 1413 3441 4785 1413
Size: 2028 1344 2363
Fragments arranged by size:
2363 2028 1344
EaeI y'GGCC_r
Cuts at: 476 812 923 1136 1460 1850 3320 3494 3885
Size: 336 111 213 324 390 1470 174 391
Cuts at: 3885 3912 5257 476
Size: 27 1345 954
Fragments arranged by size:
1470 1345 954 391 390 336 324 213
174 111 27
EagI C'GGCC_G
Cuts at: 476 3320 476
Size: 2844 2891
EarI CTCTTCn'nnn_
Cuts at: 172 824 1322 1379 1610 1642 1999 2495 3758
Size: 652 498 57 231 32 357 496 1263
Cuts at: 3758 3968 172
Size: 210 1939
Fragments arranged by size:
1939 1263 652 498 496 357 231 210
57 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3943 4685 4831 5545 3943
Size: 742 146 714 4133
Fragments arranged by size:
4133 742 714 146
EcoICRI GAG'CTC
Cuts at: 2747 2747
Size: 5735
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 558 628 967 1114 1666 1732 1801 3560
Size: 383 70 339 147 552 66 69 1759
Cuts at: 3560 3992 4345 5007 175
Size: 432 353 662 903
Fragments arranged by size:
1759 903 662 552 432 383 353 339
147 70 69 66
EcoNI CCTnn'n_nnAGG
Cuts at: 1042 1486 1042
Size: 444 5291
EcoO109I rG'GnC_Cy
Cuts at: 471 1495 1728 1988 2219 2874 471
Size: 1024 233 260 231 655 3332
Fragments arranged by size:
3332 1024 655 260 233 231
EcoRI G'AATT_C
Cuts at: 1228 2739 1228
Size: 1511 4224
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 633 801 3754 633
Size: 168 2953 2614
Fragments arranged by size:
2953 2614 168
FspI TGC'GCA
Cuts at: 886 3516 886
Size: 2630 3105
HaeII r_GCGC'y
Cuts at: 534 636 1861 3169 3417 4647 5418 534
Size: 102 1225 1308 248 1230 771 851
Fragments arranged by size:
1308 1230 1225 851 771 248 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 124 156 249 281 646 678 1494 1526 1822
Size: 32 93 32 365 32 816 32 296
Cuts at: 1822 1854 3577 3607 3609 3639 124
Size: 32 1723 30 2 30 2220
Fragments arranged by size:
2220 1723 816 365 296 93 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2774 2774
Size: 5735
KasI G'GCGC_C
Cuts at: 1857 3165 3413 1857
Size: 1308 248 4179
Fragments arranged by size:
4179 1308 248
KpnI G_GTAC'C
Cuts at: 2755 2755
Size: 5735
MluI A'CGCG_T
Cuts at: 5168 5168
Size: 5735
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 179 343 4494 4678 179
Size: 164 4151 184 1236
Fragments arranged by size:
4151 1236 184 164
MscI TGG'CCA
Cuts at: 814 1138 1462 1852 3496 814
Size: 324 324 390 1644 3053
Fragments arranged by size:
3053 1644 390 324 324
MslI CAynn'nnrTG
Cuts at: 1422 2233 2263 2543 2629 3724 3851 1422
Size: 811 30 280 86 1095 127 3306
Fragments arranged by size:
3306 1095 811 280 127 86 30
MspA1I CmG'CkG
Cuts at: 825 1069 1979 2054 2263 2282 2846 3160 3520
Size: 244 910 75 209 19 564 314 360
Cuts at: 3520 4304 4549 825
Size: 784 245 2011
Fragments arranged by size:
2011 910 784 564 360 314 245 244
209 75 19
NaeI GCC'GGC
Cuts at: 3916 3916
Size: 5735
NarI GG'CG_CC
Cuts at: 1858 3166 3414 1858
Size: 1308 248 4179
Fragments arranged by size:
4179 1308 248
NcoI C'CATG_G
Cuts at: 1862 1862
Size: 5735
NgoMIV G'CCGG_C
Cuts at: 3914 3914
Size: 5735
NspI r_CATG'y
Cuts at: 2788 3288 4891 2788
Size: 500 1603 3632
Fragments arranged by size:
3632 1603 500
PciI A'CATG_T
Cuts at: 4887 4887
Size: 5735
PflMI CCAn_nnn'nTGG
Cuts at: 2127 2127
Size: 5735
PfoI T'CCnGG_A
Cuts at: 191 1709 1946 191
Size: 1518 237 3980
Fragments arranged by size:
3980 1518 237
PmeI GTTT'AAAC
Cuts at: 2734 2734
Size: 5735
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2455 2487 4181 4213 2455
Size: 32 1694 32 3977
Fragments arranged by size:
3977 1694 32 32
PpuMI rG'GwC_Cy
Cuts at: 2219 2219
Size: 5735
PspOMI G'GGCC_C
Cuts at: 471 1495 1987 471
Size: 1024 492 4219
Fragments arranged by size:
4219 1024 492
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1869 1901 1869
Size: 32 5703
PstI C_TGCA'G
Cuts at: 521 949 997 1796 2782 3467 521
Size: 428 48 799 986 685 2789
Fragments arranged by size:
2789 986 799 685 428 48
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5735
PvuII CAG'CTG
Cuts at: 825 1069 2263 2282 3160 3520 825
Size: 244 1194 19 878 360 3040
Fragments arranged by size:
3040 1194 878 360 244 19
RsrII CG'GwC_CG
Cuts at: 3930 3930
Size: 5735
SacI G_AGCT'C
Cuts at: 2749 2749
Size: 5735
SacII CC_GC'GG
Cuts at: 1980 1980
Size: 5735
SalI G'TCGA_C
Cuts at: 2772 2772
Size: 5735
SapI GCTCTTCn'nnn_
Cuts at: 3758 3968 3758
Size: 210 5525
SbfI CC_TGCA'GG
Cuts at: 2782 2782
Size: 5735
ScaI AGT'ACT
Cuts at: 5252 5252
Size: 5735
SexAI A'CCwGG_T
Cuts at: 1100 1100
Size: 5735
SfcI C'TryA_G
Cuts at: 33 517 945 993 1792 2384 2778 3463 4431
Size: 484 428 48 799 592 394 685 968
Cuts at: 4431 4622 33
Size: 191 1146
Fragments arranged by size:
1146 968 799 685 592 484 428 394
191 48
SfoI GGC'GCC
Cuts at: 1859 3167 3415 1859
Size: 1308 248 4179
Fragments arranged by size:
4179 1308 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5735
SmaI CCC'GGG
Cuts at: 2217 2757 2217
Size: 540 5195
SmlI C'TyrA_G
Cuts at: 172 775 1153 1834 2890 3805 4519 4781 172
Size: 603 378 681 1056 915 714 262 1126
Fragments arranged by size:
1126 1056 915 714 681 603 378 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5735
SphI G_CATG'C
Cuts at: 2788 3288 2788
Size: 500 5235
SspI AAT'ATT
Cuts at: 2517 2517
Size: 5735
StuI AGG'CCT
Cuts at: 313 2164 313
Size: 1851 3884
StyI C'CwwG_G
Cuts at: 1004 1862 2126 2869 3223 1004
Size: 858 264 743 354 3516
Fragments arranged by size:
3516 858 743 354 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 322 627 1017 1700 2596 3318 3451 3910 4119
Size: 305 390 683 896 722 133 459 209
Cuts at: 4119 322
Size: 1938
Fragments arranged by size:
1938 896 722 683 459 390 305 209
133
TatI w'GTAC_w
Cuts at: 1520 1799 5250 1520
Size: 279 3451 2005
Fragments arranged by size:
3451 2005 279
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 686 718 5252 5284 686
Size: 32 4534 32 1137
Fragments arranged by size:
4534 1137 32 32
Tth111I GACn'n_nGTC
Cuts at: 625 1764 3532 625
Size: 1139 1768 2828
Fragments arranged by size:
2828 1768 1139
XbaI T'CTAG_A
Cuts at: 1806 2766 1806
Size: 960 4775
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 822 1776 1860 3230 822
Size: 954 84 1370 3327
Fragments arranged by size:
3327 1370 954 84
XmaI C'CCGG_G
Cuts at: 2215 2755 2215
Size: 540 5195
XmnI GAAnn'nnTTC
Cuts at: 118 118
Size: 5735
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AgeI AscI AvrII BaeI BbvCI BfrBI BmtI
BsiWI BspEI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspXI SanDI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI