Restriction map for
pF1KB4712
(Circular) (Six-base) MAPSORT of: pF1KB4712.seq Check: 9122 from: 1 to: 3854
pF1KB4712 3854 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 892 892
Size: 3854
Acc65I G'GTAC_C
Cuts at: 870 870
Size: 3854
AclI AA'CG_TT
Cuts at: 260 3694 260
Size: 3434 420
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 533 1679 2111 2464 3126 499
Size: 34 1146 432 353 662 1227
Fragments arranged by size:
1227 1146 662 432 353 34
AfeI AGC'GCT
Cuts at: 163 163
Size: 3854
AflIII A'CryG_T
Cuts at: 1206 3006 3287 1206
Size: 1800 281 1773
Fragments arranged by size:
1800 1773 281
AlwNI CAG_nnn'CTG
Cuts at: 1159 2597 1159
Size: 1438 2416
ApaLI G'TGCA_C
Cuts at: 2692 2692
Size: 3854
ApoI r'AATT_y
Cuts at: 858 3207 3463 858
Size: 2349 256 1249
Fragments arranged by size:
2349 1249 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3854
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3854
AvaI C'yCGr_G
Cuts at: 874 874
Size: 3854
BamHI G'GATC_C
Cuts at: 879 879
Size: 3854
BanI G'GyrC_C
Cuts at: 870 1284 1532 1567 870
Size: 414 248 35 3157
Fragments arranged by size:
3157 414 248 35
BanII G_rGCy'C
Cuts at: 868 1898 868
Size: 1030 2824
BbeI G_GCGC'C
Cuts at: 1288 1536 1288
Size: 248 3606
BbsI GAAGACnn'nnnn_
Cuts at: 319 642 319
Size: 323 3531
BciVI GTATCCnnnnn_n'
Cuts at: 1752 2808 3854 1752
Size: 1056 1046 1752
Fragments arranged by size:
1752 1056 1046
BclI T'GATC_A
Cuts at: 431 1374 431
Size: 943 2911
BglI GCCn_nnn'nGGC
Cuts at: 145 154 145
Size: 9 3845
BglII A'GATC_T
Cuts at: 1369 3406 1369
Size: 2037 1817
BlpI GC'TnA_GC
Cuts at: 409 966 409
Size: 557 3297
Bme1580I G_kGCm'C
Cuts at: 273 1479 1572 2696 273
Size: 1206 93 1124 1431
Fragments arranged by size:
1431 1206 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 447 1066 1147 1482 3626 447
Size: 619 81 335 2144 675
Fragments arranged by size:
2144 675 619 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 3854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 306
Size: 3854
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 1909 2623 2921 1030
Size: 879 714 298 1963
Fragments arranged by size:
1963 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 442 3558 442
Size: 3116 738
BsaAI yAC'GTr
Cuts at: 1207 1207
Size: 3854
BsaBI GATnn'nnATC
Cuts at: 6 1368 3405 6
Size: 1362 2037 455
Fragments arranged by size:
2037 1362 455
BsaHI Gr'CG_yC
Cuts at: 716 1285 1533 2235 3731 716
Size: 569 248 702 1496 839
Fragments arranged by size:
1496 839 702 569 248
BsaWI w'CCGG_w
Cuts at: 523 1043 1564 2242 2653 2800 3074 523
Size: 520 521 678 411 147 274 1303
Fragments arranged by size:
1303 678 521 520 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 379 409 733 745 763 775 379
Size: 30 324 12 18 12 3458
Fragments arranged by size:
3458 324 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 772 772
Size: 3854
BseYI C'CCAG_C
Cuts at: 1279 2037 2702 3110 1279
Size: 758 665 408 2023
Fragments arranged by size:
2023 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 151
Size: 3854
BsiEI CG_ry'CG
Cuts at: 104 1396 1442 2672 104
Size: 1292 46 1230 1286
Fragments arranged by size:
1292 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 599 868 1646 1836 2696 599
Size: 269 778 190 860 1757
Fragments arranged by size:
1757 860 778 269 190
Bsp1286I G_dGCh'C
Cuts at: 273 599 868 1479 1572 1646 1836 1898 2696
Size: 326 269 611 93 74 190 62 798
Cuts at: 2696 273
Size: 1431
Fragments arranged by size:
1431 798 611 326 269 190 93 74
62
BspHI T'CATG_A
Cuts at: 2286 2286
Size: 3854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 904 1118 1420 1801 904
Size: 214 302 381 2957
Fragments arranged by size:
2957 381 302 214
BsrBI CCG'CTC
Cuts at: 50 278 2146 2200 3684 3849 50
Size: 228 1868 54 1484 165 55
Fragments arranged by size:
1868 1484 228 165 55 54
BsrDI GCAATG_nn'
Cuts at: 336 1766 336
Size: 1430 2424
BsrFI r'CCGG_y
Cuts at: 1852 2033 1852
Size: 181 3673
BssSI C'ACGA_G
Cuts at: 580 2125 2833 580
Size: 1545 708 1601
Fragments arranged by size:
1601 1545 708
BstAPI GCAn_nnn'nTGC
Cuts at: 129 1231 129
Size: 1102 2752
BstBI TT'CG_AA
Cuts at: 2215 2215
Size: 3854
BstYI r'GATC_y
Cuts at: 1 662 879 1346 1369 1704 1950 2256 2268
Size: 661 217 467 23 335 246 306 12
Cuts at: 2268 2354 3406 1
Size: 86 1052 449
Fragments arranged by size:
1052 661 467 449 335 306 246 217
86 23 12
BtgI C'CryG_G
Cuts at: 107 179 107
Size: 72 3782
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 705 1804 1985 2148 705
Size: 1099 181 163 2411
Fragments arranged by size:
2411 1099 181 163
BtsI GCAGTG_nn'
Cuts at: 1097 1097
Size: 3854
DraI TTT'AAA
Cuts at: 853 853
Size: 3854
DrdI GACnn_nn'nnGTC
Cuts at: 1560 2904 1560
Size: 1344 2510
EaeI y'GGCC_r
Cuts at: 146 176 182 518 695 1439 1613 2004 2031
Size: 30 6 336 177 744 174 391 27
Cuts at: 2031 3376 146
Size: 1345 624
Fragments arranged by size:
1345 744 624 391 336 177 174 30
27 6
EagI C'GGCC_G
Cuts at: 1439 1439
Size: 3854
EarI CTCTTCn'nnn_
Cuts at: 285 1877 2087 285
Size: 1592 210 2052
Fragments arranged by size:
2052 1592 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 172 2062 2804 2950 3664 172
Size: 1890 742 146 714 362
Fragments arranged by size:
1890 742 714 362 146
EcoICRI GAG'CTC
Cuts at: 866 866
Size: 3854
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 499 533 1679 2111 2464 3126 306
Size: 193 34 1146 432 353 662 1034
Fragments arranged by size:
1146 1034 662 432 353 193 34
EcoNI CCTnn'n_nnAGG
Cuts at: 742 742
Size: 3854
EcoO109I rG'GnC_Cy
Cuts at: 993 993
Size: 3854
EcoRI G'AATT_C
Cuts at: 858 858
Size: 3854
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1873 1873
Size: 3854
FspI TGC'GCA
Cuts at: 1635 1635
Size: 3854
HaeII r_GCGC'y
Cuts at: 165 1288 1536 2766 3537 165
Size: 1123 248 1230 771 482
Fragments arranged by size:
1230 1123 771 482 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 202 234 1696 1726 1728 1758 202
Size: 32 1462 30 2 30 2298
Fragments arranged by size:
2298 1462 32 30 30 2
HincII GTy'rAC
Cuts at: 893 893
Size: 3854
KasI G'GCGC_C
Cuts at: 1284 1532 1284
Size: 248 3606
KpnI G_GTAC'C
Cuts at: 874 874
Size: 3854
MluI A'CGCG_T
Cuts at: 3287 3287
Size: 3854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 365 2613 2797 365
Size: 2248 184 1422
Fragments arranged by size:
2248 1422 184
MscI TGG'CCA
Cuts at: 520 697 1615 520
Size: 177 918 2759
Fragments arranged by size:
2759 918 177
MslI CAynn'nnrTG
Cuts at: 1843 1970 1843
Size: 127 3727
MspA1I CmG'CkG
Cuts at: 181 965 1279 1639 2423 2668 181
Size: 784 314 360 784 245 1367
Fragments arranged by size:
1367 784 784 360 314 245
NaeI GCC'GGC
Cuts at: 2035 2035
Size: 3854
NarI GG'CG_CC
Cuts at: 1285 1533 1285
Size: 248 3606
NcoI C'CATG_G
Cuts at: 107 107
Size: 3854
NgoMIV G'CCGG_C
Cuts at: 2033 2033
Size: 3854
NspI r_CATG'y
Cuts at: 603 907 1407 3010 603
Size: 304 500 1603 1447
Fragments arranged by size:
1603 1447 500 304
PciI A'CATG_T
Cuts at: 3006 3006
Size: 3854
PfoI T'CCnGG_A
Cuts at: 283 283
Size: 3854
PmeI GTTT'AAAC
Cuts at: 853 853
Size: 3854
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2300 2332 2300
Size: 32 3822
PstI C_TGCA'G
Cuts at: 324 901 1586 324
Size: 577 685 2592
Fragments arranged by size:
2592 685 577
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3854
PvuII CAG'CTG
Cuts at: 1279 1639 1279
Size: 360 3494
RsrII CG'GwC_CG
Cuts at: 2049 2049
Size: 3854
SacI G_AGCT'C
Cuts at: 868 868
Size: 3854
SacII CC_GC'GG
Cuts at: 182 182
Size: 3854
SalI G'TCGA_C
Cuts at: 891 891
Size: 3854
SapI GCTCTTCn'nnn_
Cuts at: 285 1877 2087 285
Size: 1592 210 2052
Fragments arranged by size:
2052 1592 210
SbfI CC_TGCA'GG
Cuts at: 901 901
Size: 3854
ScaI AGT'ACT
Cuts at: 3371 3371
Size: 3854
SfcI C'TryA_G
Cuts at: 33 320 567 897 1582 2550 2741 33
Size: 287 247 330 685 968 191 1146
Fragments arranged by size:
1146 968 685 330 287 247 191
SfoI GGC'GCC
Cuts at: 1286 1534 1286
Size: 248 3606
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3854
SmaI CCC'GGG
Cuts at: 876 876
Size: 3854
SmlI C'TyrA_G
Cuts at: 1009 1924 2638 2900 1009
Size: 915 714 262 1963
Fragments arranged by size:
1963 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3854
SphI G_CATG'C
Cuts at: 907 1407 907
Size: 500 3354
StuI AGG'CCT
Cuts at: 565 629 565
Size: 64 3790
StyI C'CwwG_G
Cuts at: 107 988 1342 107
Size: 881 354 2619
Fragments arranged by size:
2619 881 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 625 1437 1570 2029 2238 625
Size: 812 133 459 209 2241
Fragments arranged by size:
2241 812 459 209 133
TatI w'GTAC_w
Cuts at: 3369 3369
Size: 3854
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 379 411 3371 3403 379
Size: 32 2960 32 830
Fragments arranged by size:
2960 830 32 32
Tth111I GACn'n_nGTC
Cuts at: 1651 1651
Size: 3854
XbaI T'CTAG_A
Cuts at: 885 885
Size: 3854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1349 1349
Size: 3854
XmaI C'CCGG_G
Cuts at: 874 874
Size: 3854
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsiWI BsmI BsmBI BspEI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI