Restriction map for
pF1KB4697
(Circular) (Six-base) MAPSORT of: pF1KB4697.seq Check: 6706 from: 1 to: 4094
pF1KB4697 4094 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1002 1002
Size: 4094
AccI GT'mk_AC
Cuts at: 581 1132 581
Size: 551 3543
Acc65I G'GTAC_C
Cuts at: 818 1110 818
Size: 292 3802
AclI AA'CG_TT
Cuts at: 3934 3934
Size: 4094
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 1060 1919 2351 3098 3366 916
Size: 144 859 432 747 268 1644
Fragments arranged by size:
1644 859 747 432 268 144
AflII C'TTAA_G
Cuts at: 772 772
Size: 4094
AflIII A'CryG_T
Cuts at: 878 1446 2550 3527 878
Size: 568 1104 977 1445
Fragments arranged by size:
1445 1104 977 568
AgeI A'CCGG_T
Cuts at: 2482 2482
Size: 4094
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 472 472
Size: 4094
AlwNI CAG_nnn'CTG
Cuts at: 138 1038 1399 2966 138
Size: 900 361 1567 1266
Fragments arranged by size:
1567 1266 900 361
ApaI G_GGCC'C
Cuts at: 263 263
Size: 4094
ApaLI G'TGCA_C
Cuts at: 2864 2864
Size: 4094
ApoI r'AATT_y
Cuts at: 766 1098 3447 3703 766
Size: 332 2349 256 1157
Fragments arranged by size:
2349 1157 332 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4094
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
AvaI C'yCGr_G
Cuts at: 606 834 1114 606
Size: 228 280 3586
Fragments arranged by size:
3586 280 228
BamHI G'GATC_C
Cuts at: 1119 1119
Size: 4094
BanI G'GyrC_C
Cuts at: 193 235 818 1110 1524 1772 1807 193
Size: 42 583 292 414 248 35 2480
Fragments arranged by size:
2480 583 414 292 248 42 35
BanII G_rGCy'C
Cuts at: 263 675 956 1108 2138 263
Size: 412 281 152 1030 2219
Fragments arranged by size:
2219 1030 412 281 152
BbeI G_GCGC'C
Cuts at: 1528 1776 1528
Size: 248 3846
BbsI GAAGACnn'nnnn_
Cuts at: 426 632 773 426
Size: 206 141 3747
Fragments arranged by size:
3747 206 141
BbvCI CC'TCA_GC
Cuts at: 445 445
Size: 4094
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 522 556 522
Size: 34 4060
BciVI GTATCCnnnnn_n'
Cuts at: 1992 2753 4094 1992
Size: 761 1341 1992
Fragments arranged by size:
1992 1341 761
BclI T'GATC_A
Cuts at: 1614 1614
Size: 4094
BglII A'GATC_T
Cuts at: 1609 3646 1609
Size: 2037 2057
BlpI GC'TnA_GC
Cuts at: 1206 1206
Size: 4094
Bme1580I G_kGCm'C
Cuts at: 263 344 1719 1812 2868 263
Size: 81 1375 93 1056 1489
Fragments arranged by size:
1489 1375 1056 93 81
BmgBI CAC'GTC
Cuts at: 879 1056 879
Size: 177 3917
BmrI ACTGGGnnnn_n'
Cuts at: 1306 1387 1722 3866 1306
Size: 81 335 2144 1534
Fragments arranged by size:
2144 1534 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 4094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 411 855 198
Size: 213 444 3437
Fragments arranged by size:
3437 444 213
Bpu10I CC'TnA_GC
Cuts at: 445 445
Size: 4094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 831 943 1270 2149 2641 2939 831
Size: 112 327 879 492 298 1986
Fragments arranged by size:
1986 879 492 327 298 112
BsaI GGTCTCn'nnnn_
Cuts at: 688 3798 688
Size: 3110 984
BsaAI yAC'GTr
Cuts at: 1447 1447
Size: 4094
BsaBI GATnn'nnATC
Cuts at: 6 1608 3645 6
Size: 1602 2037 455
Fragments arranged by size:
2037 1602 455
BsaHI Gr'CG_yC
Cuts at: 704 778 1525 1773 2475 3971 704
Size: 74 747 248 702 1496 827
Fragments arranged by size:
1496 827 747 702 248 74
BsaWI w'CCGG_w
Cuts at: 350 1283 1804 2482 2756 2903 3314 350
Size: 933 521 678 274 147 411 1130
Fragments arranged by size:
1130 933 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 397 397
Size: 4094
BseYI C'CCAG_C
Cuts at: 465 1519 2277 2854 3350 465
Size: 1054 758 577 496 1209
Fragments arranged by size:
1209 1054 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 123 152 1636 1682 2890 104
Size: 19 29 1484 46 1208 1308
Fragments arranged by size:
1484 1308 1208 46 29 19
BsiHKAI G_wGCw'C
Cuts at: 675 1108 1886 2076 2868 675
Size: 433 778 190 792 1901
Fragments arranged by size:
1901 792 778 433 190
Bsp1286I G_dGCh'C
Cuts at: 263 344 675 956 1108 1719 1812 1886 2076
Size: 81 331 281 152 611 93 74 190
Cuts at: 2076 2138 2868 263
Size: 62 730 1489
Fragments arranged by size:
1489 730 611 331 281 190 152 93
81 74 62
BspEI T'CCGG_A
Cuts at: 350 3314 350
Size: 2964 1130
BspHI T'CATG_A
Cuts at: 3270 3270
Size: 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1002 1144 1358 1660 2041 1002
Size: 142 214 302 381 3055
Fragments arranged by size:
3055 381 302 214 142
BsrBI CCG'CTC
Cuts at: 50 2386 2440 3924 4089 50
Size: 2336 54 1484 165 55
Fragments arranged by size:
2336 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2006 2006
Size: 4094
BsrFI r'CCGG_y
Cuts at: 529 821 2092 2273 2482 529
Size: 292 1271 181 209 2141
Fragments arranged by size:
2141 1271 292 209 181
BsrGI T'GTAC_A
Cuts at: 935 935
Size: 4094
BssHII G'CGCG_C
Cuts at: 218 218
Size: 4094
BssSI C'ACGA_G
Cuts at: 2365 2723 2365
Size: 358 3736
BstAPI GCAn_nnn'nTGC
Cuts at: 138 1471 138
Size: 1333 2761
BstBI TT'CG_AA
Cuts at: 2455 2455
Size: 4094
BstEII G'GTnAC_C
Cuts at: 601 963 601
Size: 362 3732
BstXI CCAn_nnnn'nTGG
Cuts at: 918 918
Size: 4094
BstYI r'GATC_y
Cuts at: 1 329 494 1119 1586 1609 1944 2190 3202
Size: 328 165 625 467 23 335 246 1012
Cuts at: 3202 3288 3300 3646 1
Size: 86 12 346 449
Fragments arranged by size:
1012 625 467 449 346 335 328 246
165 86 23 12
BtgI C'CryG_G
Cuts at: 107 123 146 248 279 698 887 917 107
Size: 16 23 102 31 419 189 30 3284
Fragments arranged by size:
3284 419 189 102 31 30 23 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 129 168 2044 2225 2388 129
Size: 39 1876 181 163 1835
Fragments arranged by size:
1876 1835 181 163 39
BtsI GCAGTG_nn'
Cuts at: 342 1337 342
Size: 995 3099
DraI TTT'AAA
Cuts at: 1093 1093
Size: 4094
DrdI GACnn_nn'nnGTC
Cuts at: 1800 2658 1800
Size: 858 3236
EaeI y'GGCC_r
Cuts at: 149 179 1679 1853 2244 2271 3616 149
Size: 30 1500 174 391 27 1345 627
Fragments arranged by size:
1500 1345 627 391 174 30 27
EagI C'GGCC_G
Cuts at: 149 1679 149
Size: 1530 2564
EarI CTCTTCn'nnn_
Cuts at: 544 792 2117 2327 544
Size: 248 1325 210 2311
Fragments arranged by size:
2311 1325 248 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2302 2612 2758 3904 2302
Size: 310 146 1146 2492
Fragments arranged by size:
2492 1146 310 146
EcoICRI GAG'CTC
Cuts at: 673 1106 673
Size: 433 3661
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 411 855 916 1060 1919 2351 3098 3366
Size: 213 444 61 144 859 432 747 268
Cuts at: 3366 198
Size: 926
Fragments arranged by size:
926 859 747 444 432 268 213 144
61
EcoO109I rG'GnC_Cy
Cuts at: 260 1035 1233 260
Size: 775 198 3121
Fragments arranged by size:
3121 775 198
EcoRI G'AATT_C
Cuts at: 766 1098 766
Size: 332 3762
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 4094
FspI TGC'GCA
Cuts at: 1875 1875
Size: 4094
HaeII r_GCGC'y
Cuts at: 834 1528 1776 2798 3777 834
Size: 694 248 1022 979 1151
Fragments arranged by size:
1151 1022 979 694 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1936 1966 1968 1998 1936
Size: 30 2 30 4032
Fragments arranged by size:
4032 30 30 2
HincII GTy'rAC
Cuts at: 1133 1133
Size: 4094
KasI G'GCGC_C
Cuts at: 1524 1772 1524
Size: 248 3846
KpnI G_GTAC'C
Cuts at: 822 1114 822
Size: 292 3802
MluI A'CGCG_T
Cuts at: 3527 3527
Size: 4094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2765 2949 2765
Size: 184 3910
MscI TGG'CCA
Cuts at: 181 1855 181
Size: 1674 2420
MslI CAynn'nnrTG
Cuts at: 916 2083 2210 916
Size: 1167 127 2800
Fragments arranged by size:
2800 1167 127
MspA1I CmG'CkG
Cuts at: 125 281 1205 1519 1879 2892 3137 125
Size: 156 924 314 360 1013 245 1082
Fragments arranged by size:
1082 1013 924 360 314 245 156
NaeI GCC'GGC
Cuts at: 531 2275 531
Size: 1744 2350
NarI GG'CG_CC
Cuts at: 1525 1773 1525
Size: 248 3846
NcoI C'CATG_G
Cuts at: 107 248 887 917 107
Size: 141 639 30 3284
Fragments arranged by size:
3284 639 141 30
NgoMIV G'CCGG_C
Cuts at: 529 2273 529
Size: 1744 2350
NspI r_CATG'y
Cuts at: 1065 1147 1647 2554 1065
Size: 82 500 907 2605
Fragments arranged by size:
2605 907 500 82
PciI A'CATG_T
Cuts at: 2550 2550
Size: 4094
PmeI GTTT'AAAC
Cuts at: 1093 1093
Size: 4094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 4062
PpuMI rG'GwC_Cy
Cuts at: 1035 1035
Size: 4094
PspOMI G'GGCC_C
Cuts at: 259 259
Size: 4094
PspXI vC'TCGA_Gb
Cuts at: 834 834
Size: 4094
PstI C_TGCA'G
Cuts at: 134 552 1013 1141 1826 134
Size: 418 461 128 685 2402
Fragments arranged by size:
2402 685 461 418 128
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4094
PvuII CAG'CTG
Cuts at: 1519 1879 1519
Size: 360 3734
RsrII CG'GwC_CG
Cuts at: 2289 2289
Size: 4094
SacI G_AGCT'C
Cuts at: 675 1108 675
Size: 433 3661
SacII CC_GC'GG
Cuts at: 126 282 126
Size: 156 3938
SalI G'TCGA_C
Cuts at: 1131 1131
Size: 4094
SapI GCTCTTCn'nnn_
Cuts at: 2117 2327 2117
Size: 210 3884
SbfI CC_TGCA'GG
Cuts at: 1013 1141 1013
Size: 128 3966
ScaI AGT'ACT
Cuts at: 3611 3611
Size: 4094
SfcI C'TryA_G
Cuts at: 33 130 498 548 813 981 1009 1137 1822
Size: 97 368 50 265 168 28 128 685
Cuts at: 1822 2815 3006 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 368 265 191 168 128
97 50 28
SfoI GGC'GCC
Cuts at: 1526 1774 1526
Size: 248 3846
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
SmaI CCC'GGG
Cuts at: 1116 1116
Size: 4094
SmlI C'TyrA_G
Cuts at: 772 834 846 958 1249 2164 2656 2918 772
Size: 62 12 112 291 915 492 262 1948
Fragments arranged by size:
1948 915 492 291 262 112 62 12
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4094
SphI G_CATG'C
Cuts at: 1065 1147 1647 1065
Size: 82 500 3512
Fragments arranged by size:
3512 500 82
StuI AGG'CCT
Cuts at: 554 862 554
Size: 308 3786
StyI C'CwwG_G
Cuts at: 107 248 887 917 1228 1582 107
Size: 141 639 30 311 354 2619
Fragments arranged by size:
2619 639 354 311 141 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 307 457 1677 1810 2269 2478 307
Size: 150 1220 133 459 209 1923
Fragments arranged by size:
1923 1220 459 209 150 133
TatI w'GTAC_w
Cuts at: 209 935 3609 209
Size: 726 2674 694
Fragments arranged by size:
2674 726 694
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3611 3643 3611
Size: 32 4062
Tth111I GACn'n_nGTC
Cuts at: 1891 1891
Size: 4094
XbaI T'CTAG_A
Cuts at: 1125 1125
Size: 4094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 246 1589 246
Size: 1343 2751
XhoI C'TCGA_G
Cuts at: 834 834
Size: 4094
XmaI C'CCGG_G
Cuts at: 1114 1114
Size: 4094
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI AscI AvrII BaeI
BfrBI BglI BmtI BsaXI BsgI BsiWI BsmI BsmBI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI