Restriction map for
pF1KB4670
(Circular) (Six-base) MAPSORT of: pF1KB4670.seq Check: 7233 from: 1 to: 4316
pF1KB4670 4316 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AatII G_ACGT'C
Cuts at: 446 446
Size: 4316
AccI GT'mk_AC
Cuts at: 1354 1354
Size: 4316
Acc65I G'GTAC_C
Cuts at: 1332 1332
Size: 4316
AclI AA'CG_TT
Cuts at: 4156 4156
Size: 4316
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1145 2141 2573 3320 3588 1145
Size: 996 432 747 268 1873
Fragments arranged by size:
1873 996 747 432 268
AflIII A'CryG_T
Cuts at: 1668 2772 3749 1668
Size: 1104 977 2235
Fragments arranged by size:
2235 1104 977
AgeI A'CCGG_T
Cuts at: 2704 2704
Size: 4316
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 146 178 146
Size: 32 4284
AlwNI CAG_nnn'CTG
Cuts at: 1621 3188 1621
Size: 1567 2749
ApaI G_GGCC'C
Cuts at: 812 812
Size: 4316
ApaLI G'TGCA_C
Cuts at: 3086 3086
Size: 4316
ApoI r'AATT_y
Cuts at: 1320 3669 3925 1320
Size: 2349 256 1711
Fragments arranged by size:
2349 1711 256
AseI AT'TA_AT
Cuts at: 20 1091 20
Size: 1071 3245
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4316
AvaI C'yCGr_G
Cuts at: 416 1336 416
Size: 920 3396
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 997 1030 997
Size: 33 4283
BamHI G'GATC_C
Cuts at: 304 1241 1341 304
Size: 937 100 3279
Fragments arranged by size:
3279 937 100
BanI G'GyrC_C
Cuts at: 353 799 1332 1746 1994 2029 353
Size: 446 533 414 248 35 2640
Fragments arranged by size:
2640 533 446 414 248 35
BanII G_rGCy'C
Cuts at: 812 1330 2360 812
Size: 518 1030 2768
Fragments arranged by size:
2768 1030 518
BbeI G_GCGC'C
Cuts at: 1750 1998 1750
Size: 248 4068
BbsI GAAGACnn'nnnn_
Cuts at: 474 474
Size: 4316
BciVI GTATCCnnnnn_n'
Cuts at: 2214 2975 4316 2214
Size: 761 1341 2214
Fragments arranged by size:
2214 1341 761
BclI T'GATC_A
Cuts at: 1836 1836
Size: 4316
BglII A'GATC_T
Cuts at: 1831 3868 1831
Size: 2037 2279
BlpI GC'TnA_GC
Cuts at: 1428 1428
Size: 4316
Bme1580I G_kGCm'C
Cuts at: 356 812 1941 2034 3090 356
Size: 456 1129 93 1056 1582
Fragments arranged by size:
1582 1129 1056 456 93
BmrI ACTGGGnnnn_n'
Cuts at: 1528 1609 1944 4088 1528
Size: 81 335 2144 1756
Fragments arranged by size:
2144 1756 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1006 2683 1006
Size: 1677 2639
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 798 446
Size: 352 3964
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1492 2371 2863 3161 1492
Size: 879 492 298 2647
Fragments arranged by size:
2647 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 176 758 4020 176
Size: 582 3262 472
Fragments arranged by size:
3262 582 472
BsaAI yAC'GTr
Cuts at: 1669 1669
Size: 4316
BsaBI GATnn'nnATC
Cuts at: 6 911 1830 3867 6
Size: 905 919 2037 455
Fragments arranged by size:
2037 919 905 455
BsaHI Gr'CG_yC
Cuts at: 320 443 1747 1995 2697 4193 320
Size: 123 1304 248 702 1496 443
Fragments arranged by size:
1496 1304 702 443 248 123
BsaWI w'CCGG_w
Cuts at: 915 1505 2026 2704 2978 3125 3536 915
Size: 590 521 678 274 147 411 1695
Fragments arranged by size:
1695 678 590 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 146 176 396 426 433 463 676 706 146
Size: 30 220 30 7 30 213 30 3756
Fragments arranged by size:
3756 220 213 30 30 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 441 714 441
Size: 273 4043
BseYI C'CCAG_C
Cuts at: 1741 2499 3076 3572 1741
Size: 758 577 496 2485
Fragments arranged by size:
2485 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 393
Size: 4316
BsiEI CG_ry'CG
Cuts at: 104 202 1858 1904 3112 104
Size: 98 1656 46 1208 1308
Fragments arranged by size:
1656 1308 1208 98 46
BsiHKAI G_wGCw'C
Cuts at: 1330 2108 2298 3090 1330
Size: 778 190 792 2556
Fragments arranged by size:
2556 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 434 677 434
Size: 243 4073
Bsp1286I G_dGCh'C
Cuts at: 356 812 1330 1941 2034 2108 2298 2360 3090
Size: 456 518 611 93 74 190 62 730
Cuts at: 3090 356
Size: 1582
Fragments arranged by size:
1582 730 611 518 456 190 93 74
62
BspEI T'CCGG_A
Cuts at: 915 3536 915
Size: 2621 1695
BspHI T'CATG_A
Cuts at: 705 977 3492 705
Size: 272 2515 1529
Fragments arranged by size:
2515 1529 272
BspMI ACCTGCnnnn'nnnn_
Cuts at: 415 483 1132 1366 1580 1882 2263 415
Size: 68 649 234 214 302 381 2468
Fragments arranged by size:
2468 649 381 302 234 214 68
BsrBI CCG'CTC
Cuts at: 50 240 839 2608 2662 4146 4311 50
Size: 190 599 1769 54 1484 165 55
Fragments arranged by size:
1769 1484 599 190 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1303 2228 1303
Size: 925 3391
BsrFI r'CCGG_y
Cuts at: 291 2314 2495 2704 291
Size: 2023 181 209 1903
Fragments arranged by size:
2023 1903 209 181
BssHII G'CGCG_C
Cuts at: 263 263
Size: 4316
BssSI C'ACGA_G
Cuts at: 2587 2945 2587
Size: 358 3958
BstAPI GCAn_nnn'nTGC
Cuts at: 1693 1693
Size: 4316
BstBI TT'CG_AA
Cuts at: 2677 2677
Size: 4316
BstYI r'GATC_y
Cuts at: 1 304 1241 1341 1808 1831 2166 2412 3424
Size: 303 937 100 467 23 335 246 1012
Cuts at: 3424 3510 3522 3868 1
Size: 86 12 346 449
Fragments arranged by size:
1012 937 467 449 346 335 303 246
100 86 23 12
BtgI C'CryG_G
Cuts at: 107 196 228 1202 107
Size: 89 32 974 3221
Fragments arranged by size:
3221 974 89 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 459 2266 2447 2610 459
Size: 1807 181 163 2165
Fragments arranged by size:
2165 1807 181 163
BtsI GCAGTG_nn'
Cuts at: 858 1559 858
Size: 701 3615
DraI TTT'AAA
Cuts at: 1315 1315
Size: 4316
DrdI GACnn_nn'nnGTC
Cuts at: 478 2022 2880 478
Size: 1544 858 1914
Fragments arranged by size:
1914 1544 858
EaeI y'GGCC_r
Cuts at: 199 293 1901 2075 2466 2493 3838 199
Size: 94 1608 174 391 27 1345 677
Fragments arranged by size:
1608 1345 677 391 174 94 27
EagI C'GGCC_G
Cuts at: 199 1901 199
Size: 1702 2614
EarI CTCTTCn'nnn_
Cuts at: 997 1019 2339 2549 997
Size: 22 1320 210 2764
Fragments arranged by size:
2764 1320 210 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 452 2524 2834 2980 4126 452
Size: 2072 310 146 1146 642
Fragments arranged by size:
2072 1146 642 310 146
EcoICRI GAG'CTC
Cuts at: 1328 1328
Size: 4316
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 798 1145 2141 2573 3320 3588 446
Size: 352 347 996 432 747 268 1174
Fragments arranged by size:
1174 996 747 432 352 347 268
EcoNI CCTnn'n_nnAGG
Cuts at: 896 896
Size: 4316
EcoO109I rG'GnC_Cy
Cuts at: 808 809 1255 1455 808
Size: 1 446 200 3669
Fragments arranged by size:
3669 446 200 1
EcoRI G'AATT_C
Cuts at: 1320 1320
Size: 4316
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 4316
FspI TGC'GCA
Cuts at: 2097 2097
Size: 4316
HaeII r_GCGC'y
Cuts at: 1750 1998 3020 3999 1750
Size: 248 1022 979 2067
Fragments arranged by size:
2067 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 296 328 396 428 433 465 676 708 766
Size: 32 68 32 5 32 211 32 58
Cuts at: 766 798 2158 2188 2190 2220 296
Size: 32 1360 30 2 30 2392
Fragments arranged by size:
2392 1360 211 68 58 32 32 32
32 32 30 30 5 2
HincII GTy'rAC
Cuts at: 315 1355 315
Size: 1040 3276
KasI G'GCGC_C
Cuts at: 1746 1994 1746
Size: 248 4068
KpnI G_GTAC'C
Cuts at: 1336 1336
Size: 4316
MluI A'CGCG_T
Cuts at: 3749 3749
Size: 4316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 479 1199 2987 3171 479
Size: 720 1788 184 1624
Fragments arranged by size:
1788 1624 720 184
MscI TGG'CCA
Cuts at: 2077 2077
Size: 4316
MslI CAynn'nnrTG
Cuts at: 281 773 1279 2305 2432 281
Size: 492 506 1026 127 2165
Fragments arranged by size:
2165 1026 506 492 127
MspA1I CmG'CkG
Cuts at: 198 310 1427 1741 2101 3114 3359 198
Size: 112 1117 314 360 1013 245 1155
Fragments arranged by size:
1155 1117 1013 360 314 245 112
NaeI GCC'GGC
Cuts at: 293 2497 293
Size: 2204 2112
NarI GG'CG_CC
Cuts at: 1747 1995 1747
Size: 248 4068
NcoI C'CATG_G
Cuts at: 107 1202 107
Size: 1095 3221
NgoMIV G'CCGG_C
Cuts at: 291 2495 291
Size: 2204 2112
NspI r_CATG'y
Cuts at: 1369 1869 2776 1369
Size: 500 907 2909
Fragments arranged by size:
2909 907 500
PasI CC'CwG_GG
Cuts at: 805 805
Size: 4316
PciI A'CATG_T
Cuts at: 2772 2772
Size: 4316
PmeI GTTT'AAAC
Cuts at: 1315 1315
Size: 4316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 146 178 3455 3487 146
Size: 32 3277 32 975
Fragments arranged by size:
3277 975 32 32
PpuMI rG'GwC_Cy
Cuts at: 1255 1255
Size: 4316
PspOMI G'GGCC_C
Cuts at: 808 808
Size: 4316
PstI C_TGCA'G
Cuts at: 1254 1363 2048 1254
Size: 109 685 3522
Fragments arranged by size:
3522 685 109
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4316
PvuII CAG'CTG
Cuts at: 1741 2101 1741
Size: 360 3956
RsrII CG'GwC_CG
Cuts at: 2511 2511
Size: 4316
SacI G_AGCT'C
Cuts at: 1330 1330
Size: 4316
SacII CC_GC'GG
Cuts at: 199 199
Size: 4316
SalI G'TCGA_C
Cuts at: 1353 1353
Size: 4316
SapI GCTCTTCn'nnn_
Cuts at: 997 1019 2339 2549 997
Size: 22 1320 210 2764
Fragments arranged by size:
2764 1320 210 22
SbfI CC_TGCA'GG
Cuts at: 1254 1363 1254
Size: 109 4207
ScaI AGT'ACT
Cuts at: 566 3833 566
Size: 3267 1049
SexAI A'CCwGG_T
Cuts at: 891 891
Size: 4316
SfcI C'TryA_G
Cuts at: 33 1250 1359 2044 3037 3228 33
Size: 1217 109 685 993 191 1121
Fragments arranged by size:
1217 1121 993 685 191 109
SfoI GGC'GCC
Cuts at: 1748 1996 1748
Size: 248 4068
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4316
SmaI CCC'GGG
Cuts at: 418 1338 418
Size: 920 3396
SmlI C'TyrA_G
Cuts at: 1471 2386 2878 3140 1471
Size: 915 492 262 2647
Fragments arranged by size:
2647 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4316
SphI G_CATG'C
Cuts at: 1369 1869 1369
Size: 500 3816
StyI C'CwwG_G
Cuts at: 107 1170 1202 1450 1804 107
Size: 1063 32 248 354 2619
Fragments arranged by size:
2619 1063 354 248 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1899 2032 2491 2700 1899
Size: 133 459 209 3515
Fragments arranged by size:
3515 459 209 133
TatI w'GTAC_w
Cuts at: 564 1110 3831 564
Size: 546 2721 1049
Fragments arranged by size:
2721 1049 546
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3833 3865 3833
Size: 32 4284
Tth111I GACn'n_nGTC
Cuts at: 667 2113 667
Size: 1446 2870
XbaI T'CTAG_A
Cuts at: 1347 1347
Size: 4316
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1811 1811
Size: 4316
XmaI C'CCGG_G
Cuts at: 416 1336 416
Size: 920 3396
ZraI GAC'GTC
Cuts at: 444 444
Size: 4316
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AscI AvrII
BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsiWI
BsmI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PshAI PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI