Restriction map for
pF1KB4641
(Circular) (Six-base) MAPSORT of: pF1KB4641.seq Check: 9163 from: 1 to: 6020
pF1KB4641 6020 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 3058 3058
Size: 6020
Acc65I G'GTAC_C
Cuts at: 3036 3036
Size: 6020
AclI AA'CG_TT
Cuts at: 1590 5860 1590
Size: 4270 1750
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1630 2381 3845 4277 4630 5292 1630
Size: 751 1464 432 353 662 2358
Fragments arranged by size:
2358 1464 751 662 432 353
AflIII A'CryG_T
Cuts at: 1109 1300 3012 3372 5172 5453 1109
Size: 191 1712 360 1800 281 1676
Fragments arranged by size:
1800 1712 1676 360 281 191
AlwNI CAG_nnn'CTG
Cuts at: 2111 3325 4763 2111
Size: 1214 1438 3368
Fragments arranged by size:
3368 1438 1214
ApaLI G'TGCA_C
Cuts at: 4858 4858
Size: 6020
ApoI r'AATT_y
Cuts at: 658 811 871 1064 1444 1842 2073 2173 2786
Size: 153 60 193 380 398 231 100 613
Cuts at: 2786 2829 3024 5373 5629 658
Size: 43 195 2349 256 1049
Fragments arranged by size:
2349 1049 613 398 380 256 231 195
193 153 100 60 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 6020
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6020
AvaI C'yCGr_G
Cuts at: 166 3040 166
Size: 2874 3146
AvrII C'CTAG_G
Cuts at: 2874 2874
Size: 6020
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2114 2147 2114
Size: 33 5987
BamHI G'GATC_C
Cuts at: 3045 3045
Size: 6020
BanI G'GyrC_C
Cuts at: 1715 2878 3036 3450 3698 3733 1715
Size: 1163 158 414 248 35 4002
Fragments arranged by size:
4002 1163 414 248 158 35
BanII G_rGCy'C
Cuts at: 221 2423 3034 4064 221
Size: 2202 611 1030 2177
Fragments arranged by size:
2202 2177 1030 611
BbeI G_GCGC'C
Cuts at: 1719 3454 3702 1719
Size: 1735 248 4037
Fragments arranged by size:
4037 1735 248
BbsI GAAGACnn'nnnn_
Cuts at: 1229 2995 1229
Size: 1766 4254
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2686 2720 2686
Size: 34 5986
BciVI GTATCCnnnnn_n'
Cuts at: 2115 3918 4974 6020 2115
Size: 1803 1056 1046 2115
Fragments arranged by size:
2115 1803 1056 1046
BclI T'GATC_A
Cuts at: 3540 3540
Size: 6020
BfrBI ATG'CAT
Cuts at: 1345 1883 2799 2803 1345
Size: 538 916 4 4562
Fragments arranged by size:
4562 916 538 4
BglII A'GATC_T
Cuts at: 1272 3535 5572 1272
Size: 2263 2037 1720
Fragments arranged by size:
2263 2037 1720
BlpI GC'TnA_GC
Cuts at: 3132 3132
Size: 6020
Bme1580I G_kGCm'C
Cuts at: 3645 3738 4862 3645
Size: 93 1124 4803
Fragments arranged by size:
4803 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2306 3232 3313 3648 5792 2306
Size: 926 81 335 2144 2534
Fragments arranged by size:
2534 2144 926 335 81
BmtI G_CTAG'C
Cuts at: 1726 1726
Size: 6020
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4387 4387
Size: 6020
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 235 535 844 1571 148
Size: 87 300 309 727 4597
Fragments arranged by size:
4597 727 309 300 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 638 1358 2474 3196 4075 4789 5087 638
Size: 720 1116 722 879 714 298 1571
Fragments arranged by size:
1571 1116 879 722 720 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 187 5724 187
Size: 5537 483
BsaAI yAC'GTr
Cuts at: 1110 1179 1303 3373 1110
Size: 69 124 2070 3757
Fragments arranged by size:
3757 2070 124 69
BsaBI GATnn'nnATC
Cuts at: 6 2630 3534 5571 6
Size: 2624 904 2037 455
Fragments arranged by size:
2624 2037 904 455
BsaHI Gr'CG_yC
Cuts at: 1716 2888 3451 3699 4401 5897 1716
Size: 1172 563 248 702 1496 1839
Fragments arranged by size:
1839 1496 1172 702 563 248
BsaWI w'CCGG_w
Cuts at: 2550 3209 3730 4408 4819 4966 5240 2550
Size: 659 521 678 411 147 274 3330
Fragments arranged by size:
3330 678 659 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 366 1157 1465 1846 2596 184
Size: 182 791 308 381 750 3608
Fragments arranged by size:
3608 791 750 381 308 182
BseYI C'CCAG_C
Cuts at: 146 492 3445 4203 4868 5276 146
Size: 346 2953 758 665 408 890
Fragments arranged by size:
2953 890 758 665 408 346
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 258
Size: 6020
BsiEI CG_ry'CG
Cuts at: 104 3562 3608 4838 104
Size: 3458 46 1230 1286
Fragments arranged by size:
3458 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 188 2229 3034 3812 4002 4862 188
Size: 2041 805 778 190 860 1346
Fragments arranged by size:
2041 1346 860 805 778 190
BsmI GAATG_Cn'
Cuts at: 224 945 2443 224
Size: 721 1498 3801
Fragments arranged by size:
3801 1498 721
BsmBI CGTCTCn'nnnn_
Cuts at: 148 491 148
Size: 343 5677
Bsp1286I G_dGCh'C
Cuts at: 188 221 2229 2423 3034 3645 3738 3812 4002
Size: 33 2008 194 611 611 93 74 190
Cuts at: 4002 4064 4862 188
Size: 62 798 1346
Fragments arranged by size:
2008 1346 798 611 611 194 190 93
74 62 33
BspEI T'CCGG_A
Cuts at: 2550 2550
Size: 6020
BspHI T'CATG_A
Cuts at: 4452 4452
Size: 6020
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1554 2483 3070 3284 3586 3967 1554
Size: 929 587 214 302 381 3607
Fragments arranged by size:
3607 929 587 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4312 4366 5850 6015 50
Size: 4262 54 1484 165 55
Fragments arranged by size:
4262 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1329 2646 2954 3932 1329
Size: 1317 308 978 3417
Fragments arranged by size:
3417 1317 978 308
BsrFI r'CCGG_y
Cuts at: 4018 4199 4018
Size: 181 5839
BssSI C'ACGA_G
Cuts at: 2367 4291 4999 2367
Size: 1924 708 3388
Fragments arranged by size:
3388 1924 708
BstAPI GCAn_nnn'nTGC
Cuts at: 996 2941 3397 996
Size: 1945 456 3619
Fragments arranged by size:
3619 1945 456
BstBI TT'CG_AA
Cuts at: 4381 4381
Size: 6020
BstEII G'GTnAC_C
Cuts at: 1541 1541
Size: 6020
BstYI r'GATC_y
Cuts at: 1 1185 1272 2619 3045 3512 3535 3870 4116
Size: 1184 87 1347 426 467 23 335 246
Cuts at: 4116 4422 4434 4520 5572 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1347 1184 1052 467 449 426 335 306
246 87 86 23 12
BtgI C'CryG_G
Cuts at: 107 115 2241 107
Size: 8 2126 3886
Fragments arranged by size:
3886 2126 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1155 1691 3970 4151 4314 1155
Size: 536 2279 181 163 2861
Fragments arranged by size:
2861 2279 536 181 163
BtsI GCAGTG_nn'
Cuts at: 3263 3263
Size: 6020
ClaI AT'CG_AT
Cuts at: 332 332
Size: 6020
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 765 800 765
Size: 35 5985
DraI TTT'AAA
Cuts at: 2991 3019 2991
Size: 28 5992
DraIII CAC_nnn'GTG
Cuts at: 1873 1873
Size: 6020
DrdI GACnn_nn'nnGTC
Cuts at: 3726 5070 3726
Size: 1344 4676
EaeI y'GGCC_r
Cuts at: 1161 2113 3605 3779 4170 4197 5542 1161
Size: 952 1492 174 391 27 1345 1639
Fragments arranged by size:
1639 1492 1345 952 391 174 27
EagI C'GGCC_G
Cuts at: 3605 3605
Size: 6020
EarI CTCTTCn'nnn_
Cuts at: 1511 1823 2156 4043 4253 1511
Size: 312 333 1887 210 3278
Fragments arranged by size:
3278 1887 333 312 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 166 2184 4228 4970 5116 5830 166
Size: 2018 2044 742 146 714 356
Fragments arranged by size:
2044 2018 742 714 356 146
EcoICRI GAG'CTC
Cuts at: 3032 3032
Size: 6020
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 235 535 844 1571 1630 2381 3845 4277
Size: 87 300 309 727 59 751 1464 432
Cuts at: 4277 4630 5292 148
Size: 353 662 876
Fragments arranged by size:
1464 876 751 727 662 432 353 309
300 87 59
EcoNI CCTnn'n_nnAGG
Cuts at: 1570 1570
Size: 6020
EcoO109I rG'GnC_Cy
Cuts at: 1103 2247 2472 3159 1103
Size: 1144 225 687 3964
Fragments arranged by size:
3964 1144 687 225
EcoRI G'AATT_C
Cuts at: 3024 3024
Size: 6020
EcoRV GAT'ATC
Cuts at: 2666 2666
Size: 6020
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1719 4039 1719
Size: 2320 3700
FspI TGC'GCA
Cuts at: 724 3801 724
Size: 3077 2943
HaeII r_GCGC'y
Cuts at: 147 1719 3454 3702 4932 5703 147
Size: 1572 1735 248 1230 771 464
Fragments arranged by size:
1735 1572 1230 771 464 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 160 192 488 520 682 714 850 882 1177
Size: 32 296 32 162 32 136 32 295
Cuts at: 1177 1209 2087 2119 2655 2687 3862 3892 3894
Size: 32 878 32 536 32 1175 30 2
Cuts at: 3894 3924 160
Size: 30 2256
Fragments arranged by size:
2256 1175 878 536 296 295 162 136
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 408 854 3059 408
Size: 446 2205 3369
Fragments arranged by size:
3369 2205 446
HindIII A'AGCT_T
Cuts at: 799 799
Size: 6020
HpaI GTT'AAC
Cuts at: 854 854
Size: 6020
KasI G'GCGC_C
Cuts at: 1715 3450 3698 1715
Size: 1735 248 4037
Fragments arranged by size:
4037 1735 248
KpnI G_GTAC'C
Cuts at: 3040 3040
Size: 6020
MfeI C'AATT_G
Cuts at: 2837 2930 2837
Size: 93 5927
MluI A'CGCG_T
Cuts at: 5453 5453
Size: 6020
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4779 4963 4779
Size: 184 5836
MscI TGG'CCA
Cuts at: 1163 2115 3781 1163
Size: 952 1666 3402
Fragments arranged by size:
3402 1666 952
MslI CAynn'nnrTG
Cuts at: 1097 4009 4136 1097
Size: 2912 127 2981
Fragments arranged by size:
2981 2912 127
MspA1I CmG'CkG
Cuts at: 117 126 1737 3131 3445 3805 4589 4834 117
Size: 9 1611 1394 314 360 784 245 1303
Fragments arranged by size:
1611 1394 1303 784 360 314 245 9
NaeI GCC'GGC
Cuts at: 4201 4201
Size: 6020
NarI GG'CG_CC
Cuts at: 1716 3451 3699 1716
Size: 1735 248 4037
Fragments arranged by size:
4037 1735 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 6020
NdeI CA'TA_TG
Cuts at: 2305 2305
Size: 6020
NgoMIV G'CCGG_C
Cuts at: 4199 4199
Size: 6020
NheI G'CTAG_C
Cuts at: 1722 1722
Size: 6020
NsiI A_TGCA'T
Cuts at: 1347 1885 2801 2805 1347
Size: 538 916 4 4562
Fragments arranged by size:
4562 916 538 4
NspI r_CATG'y
Cuts at: 492 2803 3016 3073 3573 5176 492
Size: 2311 213 57 500 1603 1336
Fragments arranged by size:
2311 1603 1336 500 213 57
PciI A'CATG_T
Cuts at: 3012 5172 3012
Size: 2160 3860
PflMI CCAn_nnn'nTGG
Cuts at: 1195 2318 1195
Size: 1123 4897
PfoI T'CCnGG_A
Cuts at: 2081 2081
Size: 6020
PmeI GTTT'AAAC
Cuts at: 3019 3019
Size: 6020
PmlI CAC'GTG
Cuts at: 1179 1179
Size: 6020
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4466 4498 4466
Size: 32 5988
PpuMI rG'GwC_Cy
Cuts at: 1103 2472 1103
Size: 1369 4651
PsiI TTA'TAA
Cuts at: 1634 1634
Size: 6020
PstI C_TGCA'G
Cuts at: 125 1937 3067 3752 125
Size: 1812 1130 685 2393
Fragments arranged by size:
2393 1812 1130 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6020
PvuII CAG'CTG
Cuts at: 126 1737 3445 3805 126
Size: 1611 1708 360 2341
Fragments arranged by size:
2341 1708 1611 360
RsrII CG'GwC_CG
Cuts at: 4215 4215
Size: 6020
SacI G_AGCT'C
Cuts at: 3034 3034
Size: 6020
SacII CC_GC'GG
Cuts at: 118 118
Size: 6020
SalI G'TCGA_C
Cuts at: 3057 3057
Size: 6020
SapI GCTCTTCn'nnn_
Cuts at: 4043 4253 4043
Size: 210 5810
SbfI CC_TGCA'GG
Cuts at: 3067 3067
Size: 6020
ScaI AGT'ACT
Cuts at: 556 1942 5537 556
Size: 1386 3595 1039
Fragments arranged by size:
3595 1386 1039
SfcI C'TryA_G
Cuts at: 33 121 1933 3063 3748 4716 4907 33
Size: 88 1812 1130 685 968 191 1146
Fragments arranged by size:
1812 1146 1130 968 685 191 88
SfoI GGC'GCC
Cuts at: 1717 3452 3700 1717
Size: 1735 248 4037
Fragments arranged by size:
4037 1735 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6020
SmaI CCC'GGG
Cuts at: 3042 3042
Size: 6020
SmlI C'TyrA_G
Cuts at: 653 1373 2489 3175 4090 4804 5066 653
Size: 720 1116 686 915 714 262 1607
Fragments arranged by size:
1607 1116 915 720 714 686 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6020
SphI G_CATG'C
Cuts at: 2803 3073 3573 2803
Size: 270 500 5250
Fragments arranged by size:
5250 500 270
SspI AAT'ATT
Cuts at: 729 729
Size: 6020
StyI C'CwwG_G
Cuts at: 107 387 620 837 2874 2883 3154 3508 107
Size: 280 233 217 2037 9 271 354 2619
Fragments arranged by size:
2619 2037 354 280 271 233 217 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1677 3603 3736 4195 4404 1677
Size: 1926 133 459 209 3293
Fragments arranged by size:
3293 1926 459 209 133
TatI w'GTAC_w
Cuts at: 554 1940 5535 554
Size: 1386 3595 1039
Fragments arranged by size:
3595 1386 1039
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1673 1705 5537 5569 1673
Size: 32 3832 32 2124
Fragments arranged by size:
3832 2124 32 32
Tth111I GACn'n_nGTC
Cuts at: 2099 3817 2099
Size: 1718 4302
XbaI T'CTAG_A
Cuts at: 3051 3051
Size: 6020
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3515 3515
Size: 6020
XmaI C'CCGG_G
Cuts at: 3040 3040
Size: 6020
XmnI GAAnn'nnTTC
Cuts at: 1856 1856
Size: 6020
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI BbvCI BglI BmgBI Bpu10I BsaXI
BsiWI BsrGI BssHII BstXI BstZ17I Bsu36I FseI FspAI
NotI NruI PacI PasI PshAI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI