Restriction map for
pF1KB4629
(Circular) (Six-base) MAPSORT of: pF1KB4629.seq Check: 5801 from: 1 to: 4436
pF1KB4629 4436 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1268 1268
Size: 4436
AccI GT'mk_AC
Cuts at: 1474 1474
Size: 4436
Acc65I G'GTAC_C
Cuts at: 1452 1452
Size: 4436
AclI AA'CG_TT
Cuts at: 1205 4276 1205
Size: 3071 1365
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 900 1062 2261 2693 3046 3708 652
Size: 248 162 1199 432 353 662 1380
Fragments arranged by size:
1380 1199 662 432 353 248 162
AflIII A'CryG_T
Cuts at: 1424 1788 3588 3869 1424
Size: 364 1800 281 1991
Fragments arranged by size:
1991 1800 364 281
AleI CACnn'nnGTG
Cuts at: 365 865 365
Size: 500 3936
AlwNI CAG_nnn'CTG
Cuts at: 1741 3179 1741
Size: 1438 2998
ApaLI G'TGCA_C
Cuts at: 835 858 964 3274 835
Size: 23 106 2310 1997
Fragments arranged by size:
2310 1997 106 23
ApoI r'AATT_y
Cuts at: 1105 1252 1440 3789 4045 1105
Size: 147 188 2349 256 1496
Fragments arranged by size:
2349 1496 256 188 147
AseI AT'TA_AT
Cuts at: 20 20
Size: 4436
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4436
AvaI C'yCGr_G
Cuts at: 1456 1456
Size: 4436
BamHI G'GATC_C
Cuts at: 1461 1461
Size: 4436
BanI G'GyrC_C
Cuts at: 732 1452 1866 2114 2149 732
Size: 720 414 248 35 3019
Fragments arranged by size:
3019 720 414 248 35
BanII G_rGCy'C
Cuts at: 186 194 253 887 1450 2480 186
Size: 8 59 634 563 1030 2142
Fragments arranged by size:
2142 1030 634 563 59 8
BbeI G_GCGC'C
Cuts at: 736 1870 2118 736
Size: 1134 248 3054
Fragments arranged by size:
3054 1134 248
BbsI GAAGACnn'nnnn_
Cuts at: 834 1378 834
Size: 544 3892
BciVI GTATCCnnnnn_n'
Cuts at: 2334 3390 4436 2334
Size: 1056 1046 2334
Fragments arranged by size:
2334 1056 1046
BclI T'GATC_A
Cuts at: 1956 1956
Size: 4436
BfrBI ATG'CAT
Cuts at: 376 376
Size: 4436
BglI GCCn_nnn'nGGC
Cuts at: 988 988
Size: 4436
BglII A'GATC_T
Cuts at: 1412 1951 3988 1412
Size: 539 2037 1860
Fragments arranged by size:
2037 1860 539
BlpI GC'TnA_GC
Cuts at: 1548 1548
Size: 4436
Bme1580I G_kGCm'C
Cuts at: 839 862 968 1248 2061 2154 3278 839
Size: 23 106 280 813 93 1124 1997
Fragments arranged by size:
1997 1124 813 280 106 93 23
BmgBI CAC'GTC
Cuts at: 840 840
Size: 4436
BmrI ACTGGGnnnn_n'
Cuts at: 523 1278 1648 1729 2064 4208 523
Size: 755 370 81 335 2144 751
Fragments arranged by size:
2144 755 751 370 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2803 2803
Size: 4436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1390 445
Size: 945 3491
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 937 1612 2491 3205 3503 937
Size: 675 879 714 298 1870
Fragments arranged by size:
1870 879 714 675 298
BsaI GGTCTCn'nnnn_
Cuts at: 865 1178 1378 4140 865
Size: 313 200 2762 1161
Fragments arranged by size:
2762 1161 313 200
BsaAI yAC'GTr
Cuts at: 1789 1789
Size: 4436
BsaBI GATnn'nnATC
Cuts at: 6 1950 3987 6
Size: 1944 2037 455
Fragments arranged by size:
2037 1944 455
BsaHI Gr'CG_yC
Cuts at: 733 1867 2115 2817 4313 733
Size: 1134 248 702 1496 856
Fragments arranged by size:
1496 1134 856 702 248
BsaWI w'CCGG_w
Cuts at: 1625 2146 2824 3235 3382 3656 1625
Size: 521 678 411 147 274 2405
Fragments arranged by size:
2405 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 166 243 163
Size: 3 77 4356
Fragments arranged by size:
4356 77 3
BseYI C'CCAG_C
Cuts at: 910 1861 2619 3284 3692 910
Size: 951 758 665 408 1654
Fragments arranged by size:
1654 951 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1378 1378
Size: 4436
BsiEI CG_ry'CG
Cuts at: 104 1978 2024 3254 104
Size: 1874 46 1230 1286
Fragments arranged by size:
1874 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 194 253 839 862 887 968 1346 1450 2228
Size: 59 586 23 25 81 378 104 778
Cuts at: 2228 2418 3278 194
Size: 190 860 1352
Fragments arranged by size:
1352 860 778 586 378 190 104 81
59 25 23
BsiWI C'GTAC_G
Cuts at: 406 406
Size: 4436
Bsp1286I G_dGCh'C
Cuts at: 186 194 253 839 862 887 968 1248 1346
Size: 8 59 586 23 25 81 280 98
Cuts at: 1346 1450 2061 2154 2228 2418 2480 3278 186
Size: 104 611 93 74 190 62 798 1344
Fragments arranged by size:
1344 798 611 586 280 190 104 98
93 81 74 62 59 25 23 8
BspHI T'CATG_A
Cuts at: 1199 2868 1199
Size: 1669 2767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1268 1331 1486 1700 2002 2383 1268
Size: 63 155 214 302 381 3321
Fragments arranged by size:
3321 381 302 214 155 63
BsrBI CCG'CTC
Cuts at: 50 2728 2782 4266 4431 50
Size: 2678 54 1484 165 55
Fragments arranged by size:
2678 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1160 2348 1160
Size: 1188 3248
BsrFI r'CCGG_y
Cuts at: 712 1187 2434 2615 712
Size: 475 1247 181 2533
Fragments arranged by size:
2533 1247 475 181
BssSI C'ACGA_G
Cuts at: 967 2707 3415 967
Size: 1740 708 1988
Fragments arranged by size:
1988 1740 708
BstAPI GCAn_nnn'nTGC
Cuts at: 479 1401 1813 479
Size: 922 412 3102
Fragments arranged by size:
3102 922 412
BstBI TT'CG_AA
Cuts at: 2797 2797
Size: 4436
BstEII G'GTnAC_C
Cuts at: 942 1335 942
Size: 393 4043
BstYI r'GATC_y
Cuts at: 1 1412 1461 1928 1951 2286 2532 2838 2850
Size: 1411 49 467 23 335 246 306 12
Cuts at: 2850 2936 3988 1
Size: 86 1052 449
Fragments arranged by size:
1411 1052 467 449 335 306 246 86
49 23 12
Bsu36I CC'TnA_GG
Cuts at: 134 134
Size: 4436
BtgI C'CryG_G
Cuts at: 107 1210 107
Size: 1103 3333
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2386 2567 2730 2386
Size: 181 163 4092
Fragments arranged by size:
4092 181 163
BtsI GCAGTG_nn'
Cuts at: 265 1038 1679 265
Size: 773 641 3022
Fragments arranged by size:
3022 773 641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1014 1049 1014
Size: 35 4401
DraI TTT'AAA
Cuts at: 1435 1435
Size: 4436
DrdI GACnn_nn'nnGTC
Cuts at: 2142 3486 2142
Size: 1344 3092
EaeI y'GGCC_r
Cuts at: 623 1166 1189 2021 2195 2586 2613 3958 623
Size: 543 23 832 174 391 27 1345 1101
Fragments arranged by size:
1345 1101 832 543 391 174 27 23
EagI C'GGCC_G
Cuts at: 2021 2021
Size: 4436
EarI CTCTTCn'nnn_
Cuts at: 140 559 931 1297 2459 2669 140
Size: 419 372 366 1162 210 1907
Fragments arranged by size:
1907 1162 419 372 366 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2644 3386 3532 4246 2644
Size: 742 146 714 2834
Fragments arranged by size:
2834 742 714 146
EcoICRI GAG'CTC
Cuts at: 192 251 885 1448 192
Size: 59 634 563 3180
Fragments arranged by size:
3180 634 563 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 652 900 1062 1390 2261 2693 3046 3708
Size: 207 248 162 328 871 432 353 662
Cuts at: 3708 445
Size: 1173
Fragments arranged by size:
1173 871 662 432 353 328 248 207
162
EcoO109I rG'GnC_Cy
Cuts at: 299 1575 299
Size: 1276 3160
EcoRI G'AATT_C
Cuts at: 1252 1440 1252
Size: 188 4248
EcoRV GAT'ATC
Cuts at: 1086 1086
Size: 4436
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 414 2455 414
Size: 2041 2395
FspI TGC'GCA
Cuts at: 1060 2217 1060
Size: 1157 3279
HaeII r_GCGC'y
Cuts at: 736 1870 2118 3348 4119 736
Size: 1134 248 1230 771 1053
Fragments arranged by size:
1230 1134 1053 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 286 318 334 366 2278 2308 2310 2340 286
Size: 32 16 32 1912 30 2 30 2382
Fragments arranged by size:
2382 1912 32 32 30 30 16 2
HincII GTy'rAC
Cuts at: 1002 1475 1002
Size: 473 3963
KasI G'GCGC_C
Cuts at: 732 1866 2114 732
Size: 1134 248 3054
Fragments arranged by size:
3054 1134 248
KpnI G_GTAC'C
Cuts at: 1456 1456
Size: 4436
MluI A'CGCG_T
Cuts at: 3869 3869
Size: 4436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 526 3195 3379 526
Size: 2669 184 1583
Fragments arranged by size:
2669 1583 184
MscI TGG'CCA
Cuts at: 2197 2197
Size: 4436
MslI CAynn'nnrTG
Cuts at: 365 377 865 1277 2425 2552 365
Size: 12 488 412 1148 127 2249
Fragments arranged by size:
2249 1148 488 412 127 12
MspA1I CmG'CkG
Cuts at: 449 851 1362 1547 1861 2221 3005 3250 449
Size: 402 511 185 314 360 784 245 1635
Fragments arranged by size:
1635 784 511 402 360 314 245 185
NaeI GCC'GGC
Cuts at: 2617 2617
Size: 4436
NarI GG'CG_CC
Cuts at: 733 1867 2115 733
Size: 1134 248 3054
Fragments arranged by size:
3054 1134 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4436
NgoMIV G'CCGG_C
Cuts at: 2615 2615
Size: 4436
NsiI A_TGCA'T
Cuts at: 378 378
Size: 4436
NspI r_CATG'y
Cuts at: 214 380 522 1428 1489 1989 3592 214
Size: 166 142 906 61 500 1603 1058
Fragments arranged by size:
1603 1058 906 500 166 142 61
PciI A'CATG_T
Cuts at: 1424 3588 1424
Size: 2164 2272
PfoI T'CCnGG_A
Cuts at: 140 244 1226 1406 140
Size: 104 982 180 3170
Fragments arranged by size:
3170 982 180 104
PmeI GTTT'AAAC
Cuts at: 1435 1435
Size: 4436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2882 2914 2882
Size: 32 4404
PstI C_TGCA'G
Cuts at: 284 1265 1310 1483 2168 284
Size: 981 45 173 685 2552
Fragments arranged by size:
2552 981 685 173 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4436
PvuII CAG'CTG
Cuts at: 449 1861 2221 449
Size: 1412 360 2664
Fragments arranged by size:
2664 1412 360
RsrII CG'GwC_CG
Cuts at: 2631 2631
Size: 4436
SacI G_AGCT'C
Cuts at: 194 253 887 1450 194
Size: 59 634 563 3180
Fragments arranged by size:
3180 634 563 59
SalI G'TCGA_C
Cuts at: 1473 1473
Size: 4436
SapI GCTCTTCn'nnn_
Cuts at: 2459 2669 2459
Size: 210 4226
SbfI CC_TGCA'GG
Cuts at: 1265 1483 1265
Size: 218 4218
ScaI AGT'ACT
Cuts at: 487 3953 487
Size: 3466 970
SexAI A'CCwGG_T
Cuts at: 938 938
Size: 4436
SfcI C'TryA_G
Cuts at: 33 280 1261 1306 1479 2164 3132 3323 33
Size: 247 981 45 173 685 968 191 1146
Fragments arranged by size:
1146 981 968 685 247 191 173 45
SfoI GGC'GCC
Cuts at: 734 1868 2116 734
Size: 1134 248 3054
Fragments arranged by size:
3054 1134 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4436
SmaI CCC'GGG
Cuts at: 1458 1458
Size: 4436
SmlI C'TyrA_G
Cuts at: 952 1591 2506 3220 3482 952
Size: 639 915 714 262 1906
Fragments arranged by size:
1906 915 714 639 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4436
SphI G_CATG'C
Cuts at: 214 1489 1989 214
Size: 1275 500 2661
Fragments arranged by size:
2661 1275 500
StuI AGG'CCT
Cuts at: 637 1196 637
Size: 559 3877
StyI C'CwwG_G
Cuts at: 107 1570 1924 107
Size: 1463 354 2619
Fragments arranged by size:
2619 1463 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1390 2019 2152 2611 2820 1390
Size: 629 133 459 209 3006
Fragments arranged by size:
3006 629 459 209 133
TatI w'GTAC_w
Cuts at: 485 608 3951 485
Size: 123 3343 970
Fragments arranged by size:
3343 970 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3953 3985 3953
Size: 32 4404
Tth111I GACn'n_nGTC
Cuts at: 2233 2233
Size: 4436
XbaI T'CTAG_A
Cuts at: 1467 1467
Size: 4436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1931 1931
Size: 4436
XmaI C'CCGG_G
Cuts at: 1456 1456
Size: 4436
XmnI GAAnn'nnTTC
Cuts at: 637 673 637
Size: 36 4400
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmtI Bpu10I BsaXI
BsmI BsmBI BspEI BsrGI BssHII BstXI BstZ17I ClaI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI