Restriction map for
pF1KB4626
(Circular) (Six-base) MAPSORT of: pF1KB4626.seq Check: 9157 from: 1 to: 5423
pF1KB4626 5423 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2331 2331
Size: 5423
AccI GT'mk_AC
Cuts at: 2461 2461
Size: 5423
Acc65I G'GTAC_C
Cuts at: 2439 2439
Size: 5423
AclI AA'CG_TT
Cuts at: 5263 5263
Size: 5423
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1441 1702 3248 3680 4033 4695 1441
Size: 261 1546 432 353 662 2169
Fragments arranged by size:
2169 1546 662 432 353 261
AflIII A'CryG_T
Cuts at: 550 980 2775 4575 4856 550
Size: 430 1795 1800 281 1117
Fragments arranged by size:
1800 1795 1117 430 281
AleI CACnn'nnGTG
Cuts at: 1048 1907 1048
Size: 859 4564
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 205 323 644 1084 1799 205
Size: 118 321 440 715 3829
Fragments arranged by size:
3829 715 440 321 118
AlwNI CAG_nnn'CTG
Cuts at: 272 447 648 2109 2331 2728 4166 272
Size: 175 201 1461 222 397 1438 1529
Fragments arranged by size:
1529 1461 1438 397 222 201 175
ApaI G_GGCC'C
Cuts at: 1359 1699 1359
Size: 340 5083
ApaLI G'TGCA_C
Cuts at: 217 298 457 1774 4261 217
Size: 81 159 1317 2487 1379
Fragments arranged by size:
2487 1379 1317 159 81
ApoI r'AATT_y
Cuts at: 2427 4776 5032 2427
Size: 2349 256 2818
Fragments arranged by size:
2818 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5423
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5423
AvaI C'yCGr_G
Cuts at: 522 1145 1234 2402 2443 522
Size: 623 89 1168 41 3502
Fragments arranged by size:
3502 1168 623 89 41
BamHI G'GATC_C
Cuts at: 1799 2448 1799
Size: 649 4774
BanI G'GyrC_C
Cuts at: 574 607 740 1336 1566 1614 2308 2439 2853
Size: 33 133 596 230 48 694 131 414
Cuts at: 2853 3101 3136 574
Size: 248 35 2861
Fragments arranged by size:
2861 694 596 414 248 230 133 131
48 35 33
BanII G_rGCy'C
Cuts at: 785 1322 1359 1496 1699 1715 2236 2306 2437
Size: 537 37 137 203 16 521 70 131
Cuts at: 2437 3467 785
Size: 1030 2741
Fragments arranged by size:
2741 1030 537 521 203 137 131 70
37 16
BbeI G_GCGC'C
Cuts at: 1340 1618 2312 2857 3105 1340
Size: 278 694 545 248 3658
Fragments arranged by size:
3658 694 545 278 248
BbsI GAAGACnn'nnnn_
Cuts at: 2006 2083 2293 2006
Size: 77 210 5136
Fragments arranged by size:
5136 210 77
BbvCI CC'TCA_GC
Cuts at: 505 1821 505
Size: 1316 4107
BciVI GTATCCnnnnn_n'
Cuts at: 3321 4377 5423 3321
Size: 1056 1046 3321
Fragments arranged by size:
3321 1056 1046
BclI T'GATC_A
Cuts at: 1163 2943 1163
Size: 1780 3643
BglI GCCn_nnn'nGGC
Cuts at: 733 1560 733
Size: 827 4596
BglII A'GATC_T
Cuts at: 868 2938 4975 868
Size: 2070 2037 1316
Fragments arranged by size:
2070 2037 1316
BlpI GC'TnA_GC
Cuts at: 946 2535 946
Size: 1589 3834
Bme1580I G_kGCm'C
Cuts at: 221 302 461 579 610 743 1073 1359 1571
Size: 81 159 118 31 133 330 286 212
Cuts at: 1571 1699 1778 1832 2153 2297 3048 3141 4265
Size: 128 79 54 321 144 751 93 1124
Cuts at: 4265 221
Size: 1379
Fragments arranged by size:
1379 1124 751 330 321 286 212 159
144 133 128 118 93 81 79 54
31
BmgBI CAC'GTC
Cuts at: 981 1779 981
Size: 798 4625
BmrI ACTGGGnnnn_n'
Cuts at: 1580 2308 2635 2716 3051 5195 1580
Size: 728 327 81 335 2144 1808
Fragments arranged by size:
2144 1808 728 335 327 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2208 3790 2208
Size: 1582 3841
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1435 1541 2239 565
Size: 870 106 698 3749
Fragments arranged by size:
3749 870 698 106
Bpu10I CC'TnA_GC
Cuts at: 505 1213 1483 1821 505
Size: 708 270 338 4107
Fragments arranged by size:
4107 708 338 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2599 3478 4192 4490 2599
Size: 879 714 298 3532
Fragments arranged by size:
3532 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2027 5127 2027
Size: 3100 2323
BsaAI yAC'GTr
Cuts at: 624 2776 624
Size: 2152 3271
BsaBI GATnn'nnATC
Cuts at: 6 2937 4974 6
Size: 2931 2037 455
Fragments arranged by size:
2931 2037 455
BsaHI Gr'CG_yC
Cuts at: 234 1337 1615 1760 2309 2854 3102 3804 5300
Size: 1103 278 145 549 545 248 702 1496
Cuts at: 5300 234
Size: 357
Fragments arranged by size:
1496 1103 702 549 545 357 278 248
145
BsaWI w'CCGG_w
Cuts at: 221 1496 1796 1802 2612 3133 3811 4222 4369
Size: 1275 300 6 810 521 678 411 147
Cuts at: 4369 4643 221
Size: 274 1001
Fragments arranged by size:
1275 1001 810 678 521 411 300 274
147 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 673 703 1242 1272 1565 1595 673
Size: 30 539 30 293 30 4501
Fragments arranged by size:
4501 539 293 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1362 1833 1362
Size: 471 4952
BseYI C'CCAG_C
Cuts at: 167 1956 1986 2053 2848 3606 4271 4679 167
Size: 1789 30 67 795 758 665 408 911
Fragments arranged by size:
1789 911 795 758 665 408 67 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 604 1474 385
Size: 219 870 4334
Fragments arranged by size:
4334 870 219
BsiEI CG_ry'CG
Cuts at: 104 375 2965 3011 4241 104
Size: 271 2590 46 1230 1286
Fragments arranged by size:
2590 1286 1230 271 46
BsiHKAI G_wGCw'C
Cuts at: 221 302 341 461 785 1496 1630 1715 1778
Size: 81 39 120 324 711 134 85 63
Cuts at: 1778 2236 2437 3215 3405 4265 221
Size: 458 201 778 190 860 1379
Fragments arranged by size:
1379 860 778 711 458 324 201 190
134 120 85 81 63 39
Bsp1286I G_dGCh'C
Cuts at: 221 302 341 461 579 610 743 785 1073
Size: 81 39 120 118 31 133 42 288
Cuts at: 1073 1322 1359 1496 1571 1630 1699 1715 1778
Size: 249 37 137 75 59 69 16 63
Cuts at: 1778 1832 2153 2236 2297 2306 2437 3048 3141
Size: 54 321 83 61 9 131 611 93
Cuts at: 3141 3215 3405 3467 4265 221
Size: 74 190 62 798 1379
Fragments arranged by size:
1379 798 611 321 288 249 190 137
133 131 120 118 93 83 81 75
74 69 63 62 61 59 54 42
39 37 31 16 9
BspEI T'CCGG_A
Cuts at: 1496 1802 1496
Size: 306 5117
BspHI T'CATG_A
Cuts at: 1463 3855 1463
Size: 2392 3031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1547 2076 2331 2406 2473 2687 2989 3370 1547
Size: 529 255 75 67 214 302 381 3600
Fragments arranged by size:
3600 529 381 302 255 214 75 67
BsrBI CCG'CTC
Cuts at: 50 130 371 3715 3769 5253 5418 50
Size: 80 241 3344 54 1484 165 55
Fragments arranged by size:
3344 1484 241 165 80 55 54
BsrDI GCAATG_nn'
Cuts at: 3335 3335
Size: 5423
BsrFI r'CCGG_y
Cuts at: 374 791 953 1333 1563 2264 3421 3602 374
Size: 417 162 380 230 701 1157 181 2195
Fragments arranged by size:
2195 1157 701 417 380 230 181 162
BssSI C'ACGA_G
Cuts at: 1884 3694 4402 1884
Size: 1810 708 2905
Fragments arranged by size:
2905 1810 708
BstAPI GCAn_nnn'nTGC
Cuts at: 297 345 2800 297
Size: 48 2455 2920
Fragments arranged by size:
2920 2455 48
BstBI TT'CG_AA
Cuts at: 3784 3784
Size: 5423
BstEII G'GTnAC_C
Cuts at: 2088 2388 2088
Size: 300 5123
BstXI CCAn_nnnn'nTGG
Cuts at: 1909 2385 1909
Size: 476 4947
BstYI r'GATC_y
Cuts at: 1 868 1514 1799 2040 2165 2448 2915 2938
Size: 867 646 285 241 125 283 467 23
Cuts at: 2938 3273 3519 3825 3837 3923 4975 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 867 646 467 449 335 306 285
283 246 241 125 86 23 12
BtgI C'CryG_G
Cuts at: 974 1610 1902 974
Size: 636 292 4495
Fragments arranged by size:
4495 636 292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2349 3373 3554 3717 2349
Size: 1024 181 163 4055
Fragments arranged by size:
4055 1024 181 163
BtsI GCAGTG_nn'
Cuts at: 646 1022 1068 1914 1951 2236 2666 646
Size: 376 46 846 37 285 430 3403
Fragments arranged by size:
3403 846 430 376 285 46 37
DraI TTT'AAA
Cuts at: 2422 2422
Size: 5423
DrdI GACnn_nn'nnGTC
Cuts at: 3129 4473 3129
Size: 1344 4079
EaeI y'GGCC_r
Cuts at: 372 705 914 3008 3182 3573 3600 4945 372
Size: 333 209 2094 174 391 27 1345 850
Fragments arranged by size:
2094 1345 850 391 333 209 174 27
EagI C'GGCC_G
Cuts at: 372 3008 372
Size: 2636 2787
EarI CTCTTCn'nnn_
Cuts at: 996 1966 2203 3446 3656 996
Size: 970 237 1243 210 2763
Fragments arranged by size:
2763 1243 970 237 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 769 1546 1612 3631 4373 4519 5233 769
Size: 777 66 2019 742 146 714 959
Fragments arranged by size:
2019 959 777 742 714 146 66
EcoICRI GAG'CTC
Cuts at: 783 1494 1713 2234 2435 783
Size: 711 219 521 201 3771
Fragments arranged by size:
3771 711 521 219 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1435 1441 1541 1702 2239 3248 3680 4033
Size: 870 6 100 161 537 1009 432 353
Cuts at: 4033 4695 565
Size: 662 1293
Fragments arranged by size:
1293 1009 870 662 537 432 353 161
100 6
EcoO109I rG'GnC_Cy
Cuts at: 1428 1695 1696 1947 2562 1428
Size: 267 1 251 615 4289
Fragments arranged by size:
4289 615 267 251 1
EcoRI G'AATT_C
Cuts at: 2427 2427
Size: 5423
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3442 3442
Size: 5423
FspI TGC'GCA
Cuts at: 1327 3204 1327
Size: 1877 3546
HaeII r_GCGC'y
Cuts at: 155 272 946 1340 1618 2261 2312 2857 3105
Size: 117 674 394 278 643 51 545 248
Cuts at: 3105 4335 5106 155
Size: 1230 771 472
Fragments arranged by size:
1230 771 674 643 545 472 394 278
248 117 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 224 256 673 705 3265 3295 3297 3327 224
Size: 32 417 32 2560 30 2 30 2320
Fragments arranged by size:
2560 2320 417 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1086 1473 2462 1086
Size: 387 989 4047
Fragments arranged by size:
4047 989 387
HindIII A'AGCT_T
Cuts at: 463 1891 463
Size: 1428 3995
HpaI GTT'AAC
Cuts at: 1086 1086
Size: 5423
KasI G'GCGC_C
Cuts at: 1336 1614 2308 2853 3101 1336
Size: 278 694 545 248 3658
Fragments arranged by size:
3658 694 545 278 248
KpnI G_GTAC'C
Cuts at: 2443 2443
Size: 5423
MluI A'CGCG_T
Cuts at: 4856 4856
Size: 5423
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4182 4366 4182
Size: 184 5239
MscI TGG'CCA
Cuts at: 916 3184 916
Size: 2268 3155
MslI CAynn'nnrTG
Cuts at: 833 979 1048 1609 1907 3412 3539 833
Size: 146 69 561 298 1505 127 2717
Fragments arranged by size:
2717 1505 561 298 146 127 69
MspA1I CmG'CkG
Cuts at: 1825 1851 2328 2385 2534 2848 3208 3992 4237
Size: 26 477 57 149 314 360 784 245
Cuts at: 4237 1825
Size: 3011
Fragments arranged by size:
3011 784 477 360 314 245 149 57
26
NaeI GCC'GGC
Cuts at: 376 3604 376
Size: 3228 2195
NarI GG'CG_CC
Cuts at: 1337 1615 2309 2854 3102 1337
Size: 278 694 545 248 3658
Fragments arranged by size:
3658 694 545 278 248
NcoI C'CATG_G
Cuts at: 974 1610 974
Size: 636 4787
NgoMIV G'CCGG_C
Cuts at: 374 3602 374
Size: 3228 2195
NspI r_CATG'y
Cuts at: 554 888 2476 2976 4579 554
Size: 334 1588 500 1603 1398
Fragments arranged by size:
1603 1588 1398 500 334
PasI CC'CwG_GG
Cuts at: 570 1702 570
Size: 1132 4291
PciI A'CATG_T
Cuts at: 550 4575 550
Size: 4025 1398
PflMI CCAn_nnn'nTGG
Cuts at: 447 750 447
Size: 303 5120
PmeI GTTT'AAAC
Cuts at: 2422 2422
Size: 5423
PmlI CAC'GTG
Cuts at: 624 624
Size: 5423
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 3869
Size: 32 5391
PpuMI rG'GwC_Cy
Cuts at: 1947 1947
Size: 5423
PspOMI G'GGCC_C
Cuts at: 1355 1695 1355
Size: 340 5083
PspXI vC'TCGA_Gb
Cuts at: 522 1234 522
Size: 712 4711
PstI C_TGCA'G
Cuts at: 151 214 293 653 941 1406 1508 1529 1544
Size: 63 79 360 288 465 102 21 15
Cuts at: 1544 1730 2273 2470 3155 151
Size: 186 543 197 685 2419
Fragments arranged by size:
2419 685 543 465 360 288 197 186
102 79 63 21 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5423
PvuII CAG'CTG
Cuts at: 1825 2328 2385 2848 3208 1825
Size: 503 57 463 360 4040
Fragments arranged by size:
4040 503 463 360 57
RsrII CG'GwC_CG
Cuts at: 3618 3618
Size: 5423
SacI G_AGCT'C
Cuts at: 785 1496 1715 2236 2437 785
Size: 711 219 521 201 3771
Fragments arranged by size:
3771 711 521 219 201
SalI G'TCGA_C
Cuts at: 2460 2460
Size: 5423
SanDI GG'GwC_CC
Cuts at: 1947 1947
Size: 5423
SapI GCTCTTCn'nnn_
Cuts at: 3446 3656 3446
Size: 210 5213
SbfI CC_TGCA'GG
Cuts at: 2470 2470
Size: 5423
ScaI AGT'ACT
Cuts at: 4940 4940
Size: 5423
SfcI C'TryA_G
Cuts at: 33 147 210 289 649 937 1402 1504 1525
Size: 114 63 79 360 288 465 102 21
Cuts at: 1525 1540 1726 2269 2466 3151 4119 4310 33
Size: 15 186 543 197 685 968 191 1146
Fragments arranged by size:
1146 968 685 543 465 360 288 197
191 186 114 102 79 63 21 15
SfoI GGC'GCC
Cuts at: 1338 1616 2310 2855 3103 1338
Size: 278 694 545 248 3658
Fragments arranged by size:
3658 694 545 278 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5423
SgrAI Cr'CCGG_yG
Cuts at: 1333 1333
Size: 5423
SmaI CCC'GGG
Cuts at: 2445 2445
Size: 5423
SmlI C'TyrA_G
Cuts at: 522 1234 2578 3493 4207 4469 522
Size: 712 1344 915 714 262 1476
Fragments arranged by size:
1476 1344 915 714 712 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5423
SphI G_CATG'C
Cuts at: 2476 2976 2476
Size: 500 4923
StuI AGG'CCT
Cuts at: 1651 1651
Size: 5423
StyI C'CwwG_G
Cuts at: 632 974 1610 2369 2557 2911 632
Size: 342 636 759 188 354 3144
Fragments arranged by size:
3144 759 636 354 342 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 562 611 729 1623 2024 2197 3006 3139 3598
Size: 49 118 894 401 173 809 133 459
Cuts at: 3598 3807 562
Size: 209 2178
Fragments arranged by size:
2178 894 809 459 401 209 173 133
118 49
TatI w'GTAC_w
Cuts at: 4938 4938
Size: 5423
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1242 1274 1565 1597 4940 4972 1242
Size: 32 291 32 3343 32 1693
Fragments arranged by size:
3343 1693 291 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 400 3220 400
Size: 2820 2603
XbaI T'CTAG_A
Cuts at: 1980 2454 1980
Size: 474 4949
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2918 2918
Size: 5423
XhoI C'TCGA_G
Cuts at: 522 1234 522
Size: 712 4711
XmaI C'CCGG_G
Cuts at: 2443 2443
Size: 5423
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PshAI PsiI PsrI SacII SexAI SfiI
SnaBI SrfI SspI SwaI XmnI ZraI