Restriction map for
pF1KB4623
(Circular) (Six-base) MAPSORT of: pF1KB4623.seq Check: 3486 from: 1 to: 4673
pF1KB4623 4673 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 1711 1711
Size: 4673
Acc65I G'GTAC_C
Cuts at: 1689 1689
Size: 4673
AclI AA'CG_TT
Cuts at: 4513 4513
Size: 4673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2498 2930 3677 3945 2498
Size: 432 747 268 3226
Fragments arranged by size:
3226 747 432 268
AflIII A'CryG_T
Cuts at: 1284 2025 3129 4106 1284
Size: 741 1104 977 1851
Fragments arranged by size:
1851 1104 977 741
AgeI A'CCGG_T
Cuts at: 3061 3061
Size: 4673
AlwNI CAG_nnn'CTG
Cuts at: 1978 3545 1978
Size: 1567 3106
ApaLI G'TGCA_C
Cuts at: 3443 3443
Size: 4673
ApoI r'AATT_y
Cuts at: 311 469 792 1677 4026 4282 311
Size: 158 323 885 2349 256 702
Fragments arranged by size:
2349 885 702 323 256 158
AseI AT'TA_AT
Cuts at: 20 20
Size: 4673
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
AvaI C'yCGr_G
Cuts at: 1693 1693
Size: 4673
BamHI G'GATC_C
Cuts at: 539 1698 539
Size: 1159 3514
BanI G'GyrC_C
Cuts at: 274 1689 2103 2351 2386 274
Size: 1415 414 248 35 2561
Fragments arranged by size:
2561 1415 414 248 35
BanII G_rGCy'C
Cuts at: 1574 1687 2717 1574
Size: 113 1030 3530
Fragments arranged by size:
3530 1030 113
BbeI G_GCGC'C
Cuts at: 2107 2355 2107
Size: 248 4425
BbsI GAAGACnn'nnnn_
Cuts at: 884 884
Size: 4673
BciVI GTATCCnnnnn_n'
Cuts at: 2571 3332 4673 2571
Size: 761 1341 2571
Fragments arranged by size:
2571 1341 761
BclI T'GATC_A
Cuts at: 2193 2193
Size: 4673
BglI GCCn_nnn'nGGC
Cuts at: 112 748 112
Size: 636 4037
BglII A'GATC_T
Cuts at: 1191 2188 4225 1191
Size: 997 2037 1639
Fragments arranged by size:
2037 1639 997
BlpI GC'TnA_GC
Cuts at: 1785 1785
Size: 4673
Bme1580I G_kGCm'C
Cuts at: 526 2298 2391 3447 526
Size: 1772 93 1056 1752
Fragments arranged by size:
1772 1752 1056 93
BmgBI CAC'GTC
Cuts at: 1287 1287
Size: 4673
BmrI ACTGGGnnnn_n'
Cuts at: 1622 1885 1966 2301 4445 1622
Size: 263 81 335 2144 1850
Fragments arranged by size:
2144 1850 335 263 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1052 3040 1052
Size: 1988 2685
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 578 857 1019 1233 368
Size: 210 279 162 214 3808
Fragments arranged by size:
3808 279 214 210 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1849 2728 3220 3518 1060
Size: 789 879 492 298 2215
Fragments arranged by size:
2215 879 789 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 447 4377 447
Size: 3930 743
BsaAI yAC'GTr
Cuts at: 2026 2026
Size: 4673
BsaBI GATnn'nnATC
Cuts at: 6 1190 2187 4224 6
Size: 1184 997 2037 455
Fragments arranged by size:
2037 1184 997 455
BsaHI Gr'CG_yC
Cuts at: 2104 2352 3054 4550 2104
Size: 248 702 1496 2227
Fragments arranged by size:
2227 1496 702 248
BsaWI w'CCGG_w
Cuts at: 959 1862 2383 3061 3335 3482 3893 959
Size: 903 521 678 274 147 411 1739
Fragments arranged by size:
1739 903 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 992 1022 992
Size: 30 4643
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 115 120 115
Size: 5 4668
BseYI C'CCAG_C
Cuts at: 2098 2856 3433 3929 2098
Size: 758 577 496 2842
Fragments arranged by size:
2842 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 566
Size: 4673
BsiEI CG_ry'CG
Cuts at: 104 2215 2261 3469 104
Size: 2111 46 1208 1308
Fragments arranged by size:
2111 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 216 1574 1687 2465 2655 3447 216
Size: 1358 113 778 190 792 1442
Fragments arranged by size:
1442 1358 792 778 190 113
BsmI GAATG_Cn'
Cuts at: 1434 1434
Size: 4673
Bsp1286I G_dGCh'C
Cuts at: 216 526 1574 1687 2298 2391 2465 2655 2717
Size: 310 1048 113 611 93 74 190 62
Cuts at: 2717 3447 216
Size: 730 1442
Fragments arranged by size:
1442 1048 730 611 310 190 113 93
74 62
BspEI T'CCGG_A
Cuts at: 959 3893 959
Size: 2934 1739
BspHI T'CATG_A
Cuts at: 3849 3849
Size: 4673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1305 1723 1937 2239 2620 1305
Size: 418 214 302 381 3358
Fragments arranged by size:
3358 418 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1036 2965 3019 4503 4668 50
Size: 986 1929 54 1484 165 55
Fragments arranged by size:
1929 1484 986 165 55 54
BsrDI GCAATG_nn'
Cuts at: 650 1274 1504 2585 650
Size: 624 230 1081 2738
Fragments arranged by size:
2738 1081 624 230
BsrFI r'CCGG_y
Cuts at: 2671 2852 3061 2671
Size: 181 209 4283
Fragments arranged by size:
4283 209 181
BsrGI T'GTAC_A
Cuts at: 903 1474 903
Size: 571 4102
BssSI C'ACGA_G
Cuts at: 2944 3302 2944
Size: 358 4315
BstAPI GCAn_nnn'nTGC
Cuts at: 2050 2050
Size: 4673
BstBI TT'CG_AA
Cuts at: 3034 3034
Size: 4673
BstEII G'GTnAC_C
Cuts at: 1330 1422 1330
Size: 92 4581
BstXI CCAn_nnnn'nTGG
Cuts at: 285 1419 285
Size: 1134 3539
BstYI r'GATC_y
Cuts at: 1 163 240 539 1191 1698 2165 2188 2523
Size: 162 77 299 652 507 467 23 335
Cuts at: 2523 2769 3781 3867 3879 4225 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 652 507 467 449 346 335 299
246 162 86 77 23 12
BtgI C'CryG_G
Cuts at: 107 159 278 1427 107
Size: 52 119 1149 3353
Fragments arranged by size:
3353 1149 119 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 202 892 1268 1324 2623 2804 2967 202
Size: 690 376 56 1299 181 163 1908
Fragments arranged by size:
1908 1299 690 376 181 163 56
BtsI GCAGTG_nn'
Cuts at: 648 1916 648
Size: 1268 3405
DraI TTT'AAA
Cuts at: 1672 1672
Size: 4673
DrdI GACnn_nn'nnGTC
Cuts at: 2379 3237 2379
Size: 858 3815
EaeI y'GGCC_r
Cuts at: 1091 2258 2432 2823 2850 4195 1091
Size: 1167 174 391 27 1345 1569
Fragments arranged by size:
1569 1345 1167 391 174 27
EagI C'GGCC_G
Cuts at: 2258 2258
Size: 4673
EarI CTCTTCn'nnn_
Cuts at: 450 1600 2696 2906 450
Size: 1150 1096 210 2217
Fragments arranged by size:
2217 1150 1096 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 766 2881 3191 3337 4483 766
Size: 2115 310 146 1146 956
Fragments arranged by size:
2115 1146 956 310 146
EcoICRI GAG'CTC
Cuts at: 1572 1685 1572
Size: 113 4560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 578 857 1019 1233 2498 2930 3677 3945
Size: 210 279 162 214 1265 432 747 268
Cuts at: 3945 368
Size: 1096
Fragments arranged by size:
1265 1096 747 432 279 268 214 210
162
EcoNI CCTnn'n_nnAGG
Cuts at: 916 916
Size: 4673
EcoO109I rG'GnC_Cy
Cuts at: 593 1812 593
Size: 1219 3454
EcoRI G'AATT_C
Cuts at: 311 1677 311
Size: 1366 3307
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4673
FspI TGC'GCA
Cuts at: 2454 2454
Size: 4673
HaeII r_GCGC'y
Cuts at: 2107 2355 3377 4356 2107
Size: 248 1022 979 2424
Fragments arranged by size:
2424 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 605 637 937 969 1381 1413 2515 2545 2547
Size: 32 300 32 412 32 1102 30 2
Cuts at: 2547 2577 605
Size: 30 2701
Fragments arranged by size:
2701 1102 412 300 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 390 1506 1559 1712 390
Size: 1116 53 153 3351
Fragments arranged by size:
3351 1116 153 53
KasI G'GCGC_C
Cuts at: 2103 2351 2103
Size: 248 4425
KpnI G_GTAC'C
Cuts at: 1693 1693
Size: 4673
MluI A'CGCG_T
Cuts at: 4106 4106
Size: 4673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3344 3528 3344
Size: 184 4489
MscI TGG'CCA
Cuts at: 1093 2434 1093
Size: 1341 3332
MslI CAynn'nnrTG
Cuts at: 1648 2662 2789 1648
Size: 1014 127 3532
Fragments arranged by size:
3532 1014 127
MspA1I CmG'CkG
Cuts at: 161 1784 2098 2458 3471 3716 161
Size: 1623 314 360 1013 245 1118
Fragments arranged by size:
1623 1118 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2854 2854
Size: 4673
NarI GG'CG_CC
Cuts at: 2104 2352 2104
Size: 248 4425
NcoI C'CATG_G
Cuts at: 107 1427 107
Size: 1320 3353
NdeI CA'TA_TG
Cuts at: 450 450
Size: 4673
NgoMIV G'CCGG_C
Cuts at: 2852 2852
Size: 4673
NspI r_CATG'y
Cuts at: 1059 1726 2226 3133 1059
Size: 667 500 907 2599
Fragments arranged by size:
2599 907 667 500
PciI A'CATG_T
Cuts at: 3129 3129
Size: 4673
PflMI CCAn_nnn'nTGG
Cuts at: 1489 1489
Size: 4673
PfoI T'CCnGG_A
Cuts at: 1230 1230
Size: 4673
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4641
PpuMI rG'GwC_Cy
Cuts at: 593 593
Size: 4673
PsiI TTA'TAA
Cuts at: 916 916
Size: 4673
PstI C_TGCA'G
Cuts at: 369 822 1720 2405 369
Size: 453 898 685 2637
Fragments arranged by size:
2637 898 685 453
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4673
PvuII CAG'CTG
Cuts at: 2098 2458 2098
Size: 360 4313
RsrII CG'GwC_CG
Cuts at: 753 2868 753
Size: 2115 2558
SacI G_AGCT'C
Cuts at: 1574 1687 1574
Size: 113 4560
SacII CC_GC'GG
Cuts at: 162 162
Size: 4673
SalI G'TCGA_C
Cuts at: 1710 1710
Size: 4673
SanDI GG'GwC_CC
Cuts at: 593 593
Size: 4673
SapI GCTCTTCn'nnn_
Cuts at: 2696 2906 2696
Size: 210 4463
SbfI CC_TGCA'GG
Cuts at: 1720 1720
Size: 4673
ScaI AGT'ACT
Cuts at: 484 1611 4190 484
Size: 1127 2579 967
Fragments arranged by size:
2579 1127 967
SexAI A'CCwGG_T
Cuts at: 175 175
Size: 4673
SfcI C'TryA_G
Cuts at: 33 365 818 1716 2401 3394 3585 33
Size: 332 453 898 685 993 191 1121
Fragments arranged by size:
1121 993 898 685 453 332 191
SfoI GGC'GCC
Cuts at: 2105 2353 2105
Size: 248 4425
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
SmaI CCC'GGG
Cuts at: 1695 1695
Size: 4673
SmlI C'TyrA_G
Cuts at: 1039 1828 2743 3235 3497 1039
Size: 789 915 492 262 2215
Fragments arranged by size:
2215 915 789 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4673
SphI G_CATG'C
Cuts at: 1726 2226 1726
Size: 500 4173
StuI AGG'CCT
Cuts at: 1583 1583
Size: 4673
StyI C'CwwG_G
Cuts at: 107 1427 1807 2161 107
Size: 1320 380 354 2619
Fragments arranged by size:
2619 1320 380 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 407 770 1604 2256 2389 2848 3057 407
Size: 363 834 652 133 459 209 2023
Fragments arranged by size:
2023 834 652 459 363 209 133
TatI w'GTAC_w
Cuts at: 482 903 1474 1609 4188 482
Size: 421 571 135 2579 967
Fragments arranged by size:
2579 967 571 421 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4190 4222 4190
Size: 32 4641
Tth111I GACn'n_nGTC
Cuts at: 2470 2470
Size: 4673
XbaI T'CTAG_A
Cuts at: 1704 1704
Size: 4673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2168 2168
Size: 4673
XmaI C'CCGG_G
Cuts at: 1693 1693
Size: 4673
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI
Bpu10I BsiWI BsmBI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PspOMI
PspXI PsrI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI