Restriction map for
pF1KB4619
(Circular) (Six-base) MAPSORT of: pF1KB4619.seq Check: 6547 from: 1 to: 4265
pF1KB4619 4265 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 967 1303 967
Size: 336 3929
Acc65I G'GTAC_C
Cuts at: 1281 1281
Size: 4265
AclI AA'CG_TT
Cuts at: 4105 4105
Size: 4265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2090 2522 2875 3537 2090
Size: 432 353 662 2818
Fragments arranged by size:
2818 662 432 353
AflIII A'CryG_T
Cuts at: 400 1617 3417 3698 400
Size: 1217 1800 281 967
Fragments arranged by size:
1800 1217 967 281
AlwNI CAG_nnn'CTG
Cuts at: 442 1570 3008 442
Size: 1128 1438 1699
Fragments arranged by size:
1699 1438 1128
ApaLI G'TGCA_C
Cuts at: 3103 3103
Size: 4265
ApoI r'AATT_y
Cuts at: 518 841 1269 3618 3874 518
Size: 323 428 2349 256 909
Fragments arranged by size:
2349 909 428 323 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4265
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
AvaI C'yCGr_G
Cuts at: 1285 1285
Size: 4265
AvrII C'CTAG_G
Cuts at: 1012 1012
Size: 4265
BamHI G'GATC_C
Cuts at: 417 936 1290 417
Size: 519 354 3392
Fragments arranged by size:
3392 519 354
BanI G'GyrC_C
Cuts at: 1281 1695 1943 1978 1281
Size: 414 248 35 3568
Fragments arranged by size:
3568 414 248 35
BanII G_rGCy'C
Cuts at: 245 1279 2309 245
Size: 1034 1030 2201
Fragments arranged by size:
2201 1034 1030
BbeI G_GCGC'C
Cuts at: 1699 1947 1699
Size: 248 4017
BciVI GTATCCnnnnn_n'
Cuts at: 301 2163 3219 4265 301
Size: 1862 1056 1046 301
Fragments arranged by size:
1862 1056 1046 301
BclI T'GATC_A
Cuts at: 1785 1785
Size: 4265
BglII A'GATC_T
Cuts at: 1780 3817 1780
Size: 2037 2228
BlpI GC'TnA_GC
Cuts at: 1377 1377
Size: 4265
Bme1580I G_kGCm'C
Cuts at: 1890 1983 3107 1890
Size: 93 1124 3048
Fragments arranged by size:
3048 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 937 1477 1558 1893 4037 937
Size: 540 81 335 2144 1165
Fragments arranged by size:
2144 1165 540 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2632 2632
Size: 4265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 752 974 1016 1226 752
Size: 222 42 210 3791
Fragments arranged by size:
3791 222 210 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1441 2320 3034 3332 1441
Size: 879 714 298 2374
Fragments arranged by size:
2374 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3969 3969
Size: 4265
BsaAI yAC'GTr
Cuts at: 1618 1618
Size: 4265
BsaBI GATnn'nnATC
Cuts at: 6 353 1779 3816 6
Size: 347 1426 2037 455
Fragments arranged by size:
2037 1426 455 347
BsaHI Gr'CG_yC
Cuts at: 383 1696 1944 2646 4142 383
Size: 1313 248 702 1496 506
Fragments arranged by size:
1496 1313 702 506 248
BsaWI w'CCGG_w
Cuts at: 1454 1975 2653 3064 3211 3485 1454
Size: 521 678 411 147 274 2234
Fragments arranged by size:
2234 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 184 214 585 615 972 1002 1124 1154 184
Size: 30 371 30 357 30 122 30 3295
Fragments arranged by size:
3295 371 357 122 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 314 229
Size: 85 4180
BseYI C'CCAG_C
Cuts at: 1690 2448 3113 3521 1690
Size: 758 665 408 2434
Fragments arranged by size:
2434 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 671
Size: 4265
BsiEI CG_ry'CG
Cuts at: 104 123 1807 1853 3083 104
Size: 19 1684 46 1230 1286
Fragments arranged by size:
1684 1286 1230 46 19
BsiHKAI G_wGCw'C
Cuts at: 245 727 1279 2057 2247 3107 245
Size: 482 552 778 190 860 1403
Fragments arranged by size:
1403 860 778 552 482 190
BsmI GAATG_Cn'
Cuts at: 877 877
Size: 4265
Bsp1286I G_dGCh'C
Cuts at: 245 727 1279 1890 1983 2057 2247 2309 3107
Size: 482 552 611 93 74 190 62 798
Cuts at: 3107 245
Size: 1403
Fragments arranged by size:
1403 798 611 552 482 190 93 74
62
BspHI T'CATG_A
Cuts at: 2697 2697
Size: 4265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1315 1529 1831 2212 1315
Size: 214 302 381 3368
Fragments arranged by size:
3368 381 302 214
BsrBI CCG'CTC
Cuts at: 50 560 2557 2611 4095 4260 50
Size: 510 1997 54 1484 165 55
Fragments arranged by size:
1997 1484 510 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1100 2177 1100
Size: 1077 3188
BsrFI r'CCGG_y
Cuts at: 2263 2444 2263
Size: 181 4084
BsrGI T'GTAC_A
Cuts at: 592 592
Size: 4265
BssSI C'ACGA_G
Cuts at: 2536 3244 2536
Size: 708 3557
BstAPI GCAn_nnn'nTGC
Cuts at: 442 1642 442
Size: 1200 3065
BstBI TT'CG_AA
Cuts at: 2626 2626
Size: 4265
BstYI r'GATC_y
Cuts at: 1 417 699 936 1290 1757 1780 2115 2361
Size: 416 282 237 354 467 23 335 246
Cuts at: 2361 2667 2679 2765 3817 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 467 449 416 354 335 306 282
246 237 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1005 1064 1005
Size: 59 4206
BtgI C'CryG_G
Cuts at: 107 176 107
Size: 69 4196
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2215 2396 2559 2215
Size: 181 163 3921
Fragments arranged by size:
3921 181 163
BtsI GCAGTG_nn'
Cuts at: 1508 1508
Size: 4265
DraI TTT'AAA
Cuts at: 425 1264 425
Size: 839 3426
DrdI GACnn_nn'nnGTC
Cuts at: 910 1971 3315 910
Size: 1061 1344 1860
Fragments arranged by size:
1860 1344 1061
EaeI y'GGCC_r
Cuts at: 120 173 429 1850 2024 2415 2442 3787 120
Size: 53 256 1421 174 391 27 1345 598
Fragments arranged by size:
1421 1345 598 391 256 174 53 27
EagI C'GGCC_G
Cuts at: 120 1850 120
Size: 1730 2535
EarI CTCTTCn'nnn_
Cuts at: 2288 2498 2288
Size: 210 4055
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2473 3215 3361 4075 2473
Size: 742 146 714 2663
Fragments arranged by size:
2663 742 714 146
EcoICRI GAG'CTC
Cuts at: 243 1277 243
Size: 1034 3231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 752 974 1016 1226 2090 2522 2875 3537 752
Size: 222 42 210 864 432 353 662 1480
Fragments arranged by size:
1480 864 662 432 353 222 210 42
EcoNI CCTnn'n_nnAGG
Cuts at: 1067 1067
Size: 4265
EcoO109I rG'GnC_Cy
Cuts at: 1009 1404 1009
Size: 395 3870
EcoRI G'AATT_C
Cuts at: 1269 1269
Size: 4265
EcoRV GAT'ATC
Cuts at: 824 1054 1165 824
Size: 230 111 3924
Fragments arranged by size:
3924 230 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1093 2284 1093
Size: 1191 3074
FspI TGC'GCA
Cuts at: 2046 2046
Size: 4265
HaeII r_GCGC'y
Cuts at: 153 1699 1947 3177 3948 153
Size: 1546 248 1230 771 470
Fragments arranged by size:
1546 1230 771 470 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2107 2137 2139 2169 2107
Size: 30 2 30 4203
Fragments arranged by size:
4203 30 30 2
HincII GTy'rAC
Cuts at: 968 1304 968
Size: 336 3929
KasI G'GCGC_C
Cuts at: 1695 1943 1695
Size: 248 4017
KpnI G_GTAC'C
Cuts at: 1285 1285
Size: 4265
MfeI C'AATT_G
Cuts at: 908 908
Size: 4265
MluI A'CGCG_T
Cuts at: 3698 3698
Size: 4265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1110 3024 3208 1110
Size: 1914 184 2167
Fragments arranged by size:
2167 1914 184
MscI TGG'CCA
Cuts at: 431 2026 431
Size: 1595 2670
MslI CAynn'nnrTG
Cuts at: 1105 2254 2381 1105
Size: 1149 127 2989
Fragments arranged by size:
2989 1149 127
MspA1I CmG'CkG
Cuts at: 1376 1690 2050 2834 3079 1376
Size: 314 360 784 245 2562
Fragments arranged by size:
2562 784 360 314 245
NaeI GCC'GGC
Cuts at: 2446 2446
Size: 4265
NarI GG'CG_CC
Cuts at: 1696 1944 1696
Size: 248 4017
NcoI C'CATG_G
Cuts at: 107 176 107
Size: 69 4196
NgoMIV G'CCGG_C
Cuts at: 2444 2444
Size: 4265
NspI r_CATG'y
Cuts at: 404 1318 1818 3421 404
Size: 914 500 1603 1248
Fragments arranged by size:
1603 1248 914 500
PciI A'CATG_T
Cuts at: 400 3417 400
Size: 3017 1248
PfoI T'CCnGG_A
Cuts at: 1047 1047
Size: 4265
PmeI GTTT'AAAC
Cuts at: 1264 1264
Size: 4265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2711 2743 2711
Size: 32 4233
PpuMI rG'GwC_Cy
Cuts at: 1009 1009
Size: 4265
PsiI TTA'TAA
Cuts at: 293 293
Size: 4265
PstI C_TGCA'G
Cuts at: 1312 1997 1312
Size: 685 3580
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4265
PvuII CAG'CTG
Cuts at: 1690 2050 1690
Size: 360 3905
RsrII CG'GwC_CG
Cuts at: 2460 2460
Size: 4265
SacI G_AGCT'C
Cuts at: 245 1279 245
Size: 1034 3231
SalI G'TCGA_C
Cuts at: 966 1302 966
Size: 336 3929
SapI GCTCTTCn'nnn_
Cuts at: 2288 2498 2288
Size: 210 4055
SbfI CC_TGCA'GG
Cuts at: 1312 1312
Size: 4265
ScaI AGT'ACT
Cuts at: 930 3782 930
Size: 2852 1413
SfcI C'TryA_G
Cuts at: 33 1308 1993 2961 3152 33
Size: 1275 685 968 191 1146
Fragments arranged by size:
1275 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1697 1945 1697
Size: 248 4017
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
SmaI CCC'GGG
Cuts at: 1287 1287
Size: 4265
SmlI C'TyrA_G
Cuts at: 1420 2335 3049 3311 1420
Size: 915 714 262 2374
Fragments arranged by size:
2374 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4265
SphI G_CATG'C
Cuts at: 1318 1818 1318
Size: 500 3765
StuI AGG'CCT
Cuts at: 1017 1017
Size: 4265
StyI C'CwwG_G
Cuts at: 107 176 1012 1399 1753 107
Size: 69 836 387 354 2619
Fragments arranged by size:
2619 836 387 354 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 349 1848 1981 2440 2649 349
Size: 1499 133 459 209 1965
Fragments arranged by size:
1965 1499 459 209 133
TatI w'GTAC_w
Cuts at: 191 592 928 3780 191
Size: 401 336 2852 676
Fragments arranged by size:
2852 676 401 336
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3782 3814 3782
Size: 32 4233
Tth111I GACn'n_nGTC
Cuts at: 2062 2062
Size: 4265
XbaI T'CTAG_A
Cuts at: 1296 1296
Size: 4265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1760 1760
Size: 4265
XmaI C'CCGG_G
Cuts at: 1285 1285
Size: 4265
XmnI GAAnn'nnTTC
Cuts at: 518 518
Size: 4265
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsiWI BsmBI BspEI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI