Restriction map for
pF1KB4618
(Circular) (Six-base) MAPSORT of: pF1KB4618.seq Check: 5716 from: 1 to: 5351
pF1KB4618 5351 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 2014 2389 2014
Size: 375 4976
Acc65I G'GTAC_C
Cuts at: 2367 2367
Size: 5351
AclI AA'CG_TT
Cuts at: 5191 5191
Size: 5351
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1271 1634 2219 2338 3176 3608 3961 4623 1271
Size: 363 585 119 838 432 353 662 1999
Fragments arranged by size:
1999 838 662 585 432 363 353 119
AfeI AGC'GCT
Cuts at: 1087 1087
Size: 5351
AflIII A'CryG_T
Cuts at: 455 457 761 2703 4503 4784 455
Size: 2 304 1942 1800 281 1022
Fragments arranged by size:
1942 1800 1022 304 281 2
AgeI A'CCGG_T
Cuts at: 1874 1874
Size: 5351
AleI CACnn'nnGTG
Cuts at: 1615 1615
Size: 5351
AlwNI CAG_nnn'CTG
Cuts at: 1789 2656 4094 1789
Size: 867 1438 3046
Fragments arranged by size:
3046 1438 867
ApaI G_GGCC'C
Cuts at: 822 822
Size: 5351
ApaLI G'TGCA_C
Cuts at: 2160 4189 2160
Size: 2029 3322
ApoI r'AATT_y
Cuts at: 1864 2143 2355 4704 4960 1864
Size: 279 212 2349 256 2255
Fragments arranged by size:
2349 2255 279 256 212
AseI AT'TA_AT
Cuts at: 20 20
Size: 5351
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5351
AvaI C'yCGr_G
Cuts at: 572 1890 2371 572
Size: 1318 481 3552
Fragments arranged by size:
3552 1318 481
BamHI G'GATC_C
Cuts at: 2376 2376
Size: 5351
BanI G'GyrC_C
Cuts at: 114 1626 2367 2781 3029 3064 114
Size: 1512 741 414 248 35 2401
Fragments arranged by size:
2401 1512 741 414 248 35
BanII G_rGCy'C
Cuts at: 822 1164 2365 3395 822
Size: 342 1201 1030 2778
Fragments arranged by size:
2778 1201 1030 342
BbeI G_GCGC'C
Cuts at: 2785 3033 2785
Size: 248 5103
BbsI GAAGACnn'nnnn_
Cuts at: 253 1098 2232 253
Size: 845 1134 3372
Fragments arranged by size:
3372 1134 845
BbvCI CC'TCA_GC
Cuts at: 905 905
Size: 5351
BciVI GTATCCnnnnn_n'
Cuts at: 3249 4305 5351 3249
Size: 1056 1046 3249
Fragments arranged by size:
3249 1056 1046
BclI T'GATC_A
Cuts at: 2871 2871
Size: 5351
BglI GCCn_nnn'nGGC
Cuts at: 826 826
Size: 5351
BglII A'GATC_T
Cuts at: 2866 4903 2866
Size: 2037 3314
BlpI GC'TnA_GC
Cuts at: 2463 2463
Size: 5351
Bme1580I G_kGCm'C
Cuts at: 822 1631 2164 2976 3069 4193 822
Size: 809 533 812 93 1124 1980
Fragments arranged by size:
1980 1124 812 809 533 93
BmrI ACTGGGnnnn_n'
Cuts at: 1625 1985 2055 2563 2644 2979 5123 1625
Size: 360 70 508 81 335 2144 1853
Fragments arranged by size:
2144 1853 508 360 335 81 70
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3718 3718
Size: 5351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 991 1664 791
Size: 200 673 4478
Fragments arranged by size:
4478 673 200
Bpu10I CC'TnA_GC
Cuts at: 905 905
Size: 5351
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1535 2242 2527 3406 4120 4418 1535
Size: 707 285 879 714 298 2468
Fragments arranged by size:
2468 879 714 707 298 285
BsaI GGTCTCn'nnnn_
Cuts at: 976 5055 976
Size: 4079 1272
BsaAI yAC'GTr
Cuts at: 458 534 848 2704 458
Size: 76 314 1856 3105
Fragments arranged by size:
3105 1856 314 76
BsaBI GATnn'nnATC
Cuts at: 6 470 2865 4902 6
Size: 464 2395 2037 455
Fragments arranged by size:
2395 2037 464 455
BsaHI Gr'CG_yC
Cuts at: 548 2782 3030 3732 5228 548
Size: 2234 248 702 1496 671
Fragments arranged by size:
2234 1496 702 671 248
BsaWI w'CCGG_w
Cuts at: 1439 1874 2540 3061 3739 4150 4297 4571 1439
Size: 435 666 521 678 411 147 274 2219
Fragments arranged by size:
2219 678 666 521 435 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 553 583 1013 1043 1511 1541 553
Size: 30 430 30 468 30 4363
Fragments arranged by size:
4363 468 430 30 30 30
BseYI C'CCAG_C
Cuts at: 716 1167 2776 3534 4199 4607 716
Size: 451 1609 758 665 408 1460
Fragments arranged by size:
1609 1460 758 665 451 408
BsiEI CG_ry'CG
Cuts at: 104 1584 2893 2939 4169 104
Size: 1480 1309 46 1230 1286
Fragments arranged by size:
1480 1309 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1164 1973 2164 2365 3143 3333 4193 1164
Size: 809 191 201 778 190 860 2322
Fragments arranged by size:
2322 860 809 778 201 191 190
BsmI GAATG_Cn'
Cuts at: 455 878 455
Size: 423 4928
BsmBI CGTCTCn'nnnn_
Cuts at: 555 555
Size: 5351
Bsp1286I G_dGCh'C
Cuts at: 822 1164 1631 1973 2164 2365 2976 3069 3143
Size: 342 467 342 191 201 611 93 74
Cuts at: 3143 3333 3395 4193 822
Size: 190 62 798 1980
Fragments arranged by size:
1980 798 611 467 342 342 201 191
190 93 74 62
BspHI T'CATG_A
Cuts at: 3783 3783
Size: 5351
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2401 2615 2917 3298 2401
Size: 214 302 381 4454
Fragments arranged by size:
4454 381 302 214
BsrBI CCG'CTC
Cuts at: 50 429 3643 3697 5181 5346 50
Size: 379 3214 54 1484 165 55
Fragments arranged by size:
3214 1484 379 165 55 54
BsrDI GCAATG_nn'
Cuts at: 302 1220 1406 3263 302
Size: 918 186 1857 2390
Fragments arranged by size:
2390 1857 918 186
BsrFI r'CCGG_y
Cuts at: 390 857 1874 3349 3530 390
Size: 467 1017 1475 181 2211
Fragments arranged by size:
2211 1475 1017 467 181
BsrGI T'GTAC_A
Cuts at: 1310 2291 1310
Size: 981 4370
BssSI C'ACGA_G
Cuts at: 3622 4330 3622
Size: 708 4643
BstAPI GCAn_nnn'nTGC
Cuts at: 2728 2728
Size: 5351
BstBI TT'CG_AA
Cuts at: 3712 3712
Size: 5351
BstEII G'GTnAC_C
Cuts at: 1885 2127 1885
Size: 242 5109
BstXI CCAn_nnnn'nTGG
Cuts at: 1413 1413
Size: 5351
BstYI r'GATC_y
Cuts at: 1 435 912 1442 2376 2843 2866 3201 3447
Size: 434 477 530 934 467 23 335 246
Cuts at: 3447 3753 3765 3851 4903 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 934 530 477 467 449 434 335
306 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 2015 2015
Size: 5351
BtgI C'CryG_G
Cuts at: 107 139 937 1964 107
Size: 32 798 1027 3494
Fragments arranged by size:
3494 1027 798 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 724 1202 3301 3482 3645 724
Size: 478 2099 181 163 2430
Fragments arranged by size:
2430 2099 478 181 163
BtsI GCAGTG_nn'
Cuts at: 682 2594 682
Size: 1912 3439
ClaI AT'CG_AT
Cuts at: 662 662
Size: 5351
DraI TTT'AAA
Cuts at: 2350 2350
Size: 5351
DraIII CAC_nnn'GTG
Cuts at: 2202 2202
Size: 5351
DrdI GACnn_nn'nnGTC
Cuts at: 547 3057 4401 547
Size: 2510 1344 1497
Fragments arranged by size:
2510 1497 1344
EaeI y'GGCC_r
Cuts at: 136 596 1415 1796 2096 2936 3110 3501 3528
Size: 460 819 381 300 840 174 391 27
Cuts at: 3528 4873 136
Size: 1345 614
Fragments arranged by size:
1345 840 819 614 460 391 381 300
174 27
EagI C'GGCC_G
Cuts at: 2936 2936
Size: 5351
EarI CTCTTCn'nnn_
Cuts at: 129 167 283 1261 1528 1603 2314 3374 3584
Size: 38 116 978 267 75 711 1060 210
Cuts at: 3584 129
Size: 1896
Fragments arranged by size:
1896 1060 978 711 267 210 116 75
38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3559 4301 4447 5161 3559
Size: 742 146 714 3749
Fragments arranged by size:
3749 742 714 146
EcoICRI GAG'CTC
Cuts at: 1162 2363 1162
Size: 1201 4150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 991 1271 1634 1664 2219 2338 3176 3608
Size: 200 280 363 30 555 119 838 432
Cuts at: 3608 3961 4623 791
Size: 353 662 1519
Fragments arranged by size:
1519 838 662 555 432 363 353 280
200 119 30
EcoNI CCTnn'n_nnAGG
Cuts at: 1060 1060
Size: 5351
EcoO109I rG'GnC_Cy
Cuts at: 223 775 818 819 993 2490 223
Size: 552 43 1 174 1497 3084
Fragments arranged by size:
3084 1497 552 174 43 1
EcoRI G'AATT_C
Cuts at: 2355 2355
Size: 5351
EcoRV GAT'ATC
Cuts at: 1812 2037 1812
Size: 225 5126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3370 3370
Size: 5351
FspI TGC'GCA
Cuts at: 688 3132 688
Size: 2444 2907
HaeII r_GCGC'y
Cuts at: 1089 2785 3033 4263 5034 1089
Size: 1696 248 1230 771 1406
Fragments arranged by size:
1696 1406 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2238 2270 3193 3223 3225 3255 2238
Size: 32 923 30 2 30 4334
Fragments arranged by size:
4334 923 32 30 30 2
HincII GTy'rAC
Cuts at: 2390 2390
Size: 5351
KasI G'GCGC_C
Cuts at: 2781 3029 2781
Size: 248 5103
KpnI G_GTAC'C
Cuts at: 2371 2371
Size: 5351
MluI A'CGCG_T
Cuts at: 4784 4784
Size: 5351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4110 4294 4110
Size: 184 5167
MscI TGG'CCA
Cuts at: 138 598 1417 1798 2098 3112 138
Size: 460 819 381 300 1014 2377
Fragments arranged by size:
2377 1014 819 460 381 300
MslI CAynn'nnrTG
Cuts at: 112 313 664 1615 3340 3467 112
Size: 201 351 951 1725 127 1996
Fragments arranged by size:
1996 1725 951 351 201 127
MspA1I CmG'CkG
Cuts at: 904 1533 2462 2776 3136 3920 4165 904
Size: 629 929 314 360 784 245 2090
Fragments arranged by size:
2090 929 784 629 360 314 245
NaeI GCC'GGC
Cuts at: 3532 3532
Size: 5351
NarI GG'CG_CC
Cuts at: 2782 3030 2782
Size: 248 5103
NcoI C'CATG_G
Cuts at: 107 1964 107
Size: 1857 3494
NdeI CA'TA_TG
Cuts at: 1452 2060 1452
Size: 608 4743
NgoMIV G'CCGG_C
Cuts at: 3530 3530
Size: 5351
NspI r_CATG'y
Cuts at: 318 765 2404 2904 4507 318
Size: 447 1639 500 1603 1162
Fragments arranged by size:
1639 1603 1162 500 447
PciI A'CATG_T
Cuts at: 761 4503 761
Size: 3742 1609
PmeI GTTT'AAAC
Cuts at: 2350 2350
Size: 5351
PmlI CAC'GTG
Cuts at: 458 458
Size: 5351
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3797 3829 3797
Size: 32 5319
PpuMI rG'GwC_Cy
Cuts at: 223 775 993 223
Size: 552 218 4581
Fragments arranged by size:
4581 552 218
PsiI TTA'TAA
Cuts at: 788 788
Size: 5351
PspOMI G'GGCC_C
Cuts at: 818 818
Size: 5351
PstI C_TGCA'G
Cuts at: 1427 2398 3083 1427
Size: 971 685 3695
Fragments arranged by size:
3695 971 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5351
PvuII CAG'CTG
Cuts at: 904 2776 3136 904
Size: 1872 360 3119
Fragments arranged by size:
3119 1872 360
RsrII CG'GwC_CG
Cuts at: 3546 3546
Size: 5351
SacI G_AGCT'C
Cuts at: 1164 2365 1164
Size: 1201 4150
SalI G'TCGA_C
Cuts at: 2388 2388
Size: 5351
SapI GCTCTTCn'nnn_
Cuts at: 129 2314 3374 3584 129
Size: 2185 1060 210 1896
Fragments arranged by size:
2185 1896 1060 210
SbfI CC_TGCA'GG
Cuts at: 2398 2398
Size: 5351
ScaI AGT'ACT
Cuts at: 1037 4868 1037
Size: 3831 1520
SexAI A'CCwGG_T
Cuts at: 930 1100 930
Size: 170 5181
SfcI C'TryA_G
Cuts at: 33 888 1126 1423 2394 3079 4047 4238 33
Size: 855 238 297 971 685 968 191 1146
Fragments arranged by size:
1146 971 968 855 685 297 238 191
SfoI GGC'GCC
Cuts at: 2783 3031 2783
Size: 248 5103
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5351
SmaI CCC'GGG
Cuts at: 574 1892 2373 574
Size: 1318 481 3552
Fragments arranged by size:
3552 1318 481
SmlI C'TyrA_G
Cuts at: 1550 2257 2506 3421 4135 4397 1550
Size: 707 249 915 714 262 2504
Fragments arranged by size:
2504 915 714 707 262 249
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5351
SphI G_CATG'C
Cuts at: 2404 2904 2404
Size: 500 4851
StyI C'CwwG_G
Cuts at: 107 1622 1964 2485 2839 107
Size: 1515 342 521 354 2619
Fragments arranged by size:
2619 1515 521 354 342
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 192 1598 2934 3067 3526 3735 192
Size: 1406 1336 133 459 209 1808
Fragments arranged by size:
1808 1406 1336 459 209 133
TatI w'GTAC_w
Cuts at: 983 1035 1310 2291 4866 983
Size: 52 275 981 2575 1468
Fragments arranged by size:
2575 1468 981 275 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1013 1045 1511 1543 4868 4900 1013
Size: 32 466 32 3325 32 1464
Fragments arranged by size:
3325 1464 466 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 932 2284 3148 932
Size: 1352 864 3135
Fragments arranged by size:
3135 1352 864
XbaI T'CTAG_A
Cuts at: 2382 2382
Size: 5351
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 291 1015 2088 2846 291
Size: 724 1073 758 2796
Fragments arranged by size:
2796 1073 758 724
XmaI C'CCGG_G
Cuts at: 572 1890 2371 572
Size: 1318 481 3552
Fragments arranged by size:
3552 1318 481
XmnI GAAnn'nnTTC
Cuts at: 1790 2323 1790
Size: 533 4818
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PmeI PmlI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BseRI BsgI BsiWI
BspEI BssHII Bsu36I CspCI FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PshAI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI