Restriction map for
pF1KB4604
(Circular) (Six-base) MAPSORT of: pF1KB4604.seq Check: 8409 from: 1 to: 4022
pF1KB4604 4022 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1060 1060
Size: 4022
Acc65I G'GTAC_C
Cuts at: 842 1038 842
Size: 196 3826
AclI AA'CG_TT
Cuts at: 3862 3862
Size: 4022
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1847 2279 3026 3294 1847
Size: 432 747 268 2575
Fragments arranged by size:
2575 747 432 268
AflIII A'CryG_T
Cuts at: 782 1374 2478 3455 782
Size: 592 1104 977 1349
Fragments arranged by size:
1349 1104 977 592
AgeI A'CCGG_T
Cuts at: 2410 2410
Size: 4022
AlwNI CAG_nnn'CTG
Cuts at: 687 1327 2894 687
Size: 640 1567 1815
Fragments arranged by size:
1815 1567 640
ApaLI G'TGCA_C
Cuts at: 495 2792 495
Size: 2297 1725
ApoI r'AATT_y
Cuts at: 268 592 1026 3375 3631 268
Size: 324 434 2349 256 659
Fragments arranged by size:
2349 659 434 324 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4022
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
AvaI C'yCGr_G
Cuts at: 758 1042 758
Size: 284 3738
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 212 245 212
Size: 33 3989
BamHI G'GATC_C
Cuts at: 1047 1047
Size: 4022
BanI G'GyrC_C
Cuts at: 508 842 1038 1452 1700 1735 508
Size: 334 196 414 248 35 2795
Fragments arranged by size:
2795 414 334 248 196 35
BanII G_rGCy'C
Cuts at: 1036 2066 1036
Size: 1030 2992
BbeI G_GCGC'C
Cuts at: 1456 1704 1456
Size: 248 3774
BbvCI CC'TCA_GC
Cuts at: 164 164
Size: 4022
BciVI GTATCCnnnnn_n'
Cuts at: 1920 2681 4022 1920
Size: 761 1341 1920
Fragments arranged by size:
1920 1341 761
BclI T'GATC_A
Cuts at: 1542 1542
Size: 4022
BglI GCCn_nnn'nGGC
Cuts at: 112 148 112
Size: 36 3986
BglII A'GATC_T
Cuts at: 1537 3574 1537
Size: 2037 1985
BlpI GC'TnA_GC
Cuts at: 1134 1134
Size: 4022
Bme1580I G_kGCm'C
Cuts at: 499 511 1647 1740 2796 499
Size: 12 1136 93 1056 1725
Fragments arranged by size:
1725 1136 1056 93 12
BmrI ACTGGGnnnn_n'
Cuts at: 1234 1315 1650 3794 1234
Size: 81 335 2144 1462
Fragments arranged by size:
2144 1462 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2389 2389
Size: 4022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 745 523
Size: 222 3800
Bpu10I CC'TnA_GC
Cuts at: 164 164
Size: 4022
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 1198 2077 2569 2867 604
Size: 594 879 492 298 1759
Fragments arranged by size:
1759 879 594 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 728 913 3726 728
Size: 185 2813 1024
Fragments arranged by size:
2813 1024 185
BsaAI yAC'GTr
Cuts at: 1375 1375
Size: 4022
BsaBI GATnn'nnATC
Cuts at: 6 1536 3573 6
Size: 1530 2037 455
Fragments arranged by size:
2037 1530 455
BsaHI Gr'CG_yC
Cuts at: 1453 1701 2403 3899 1453
Size: 248 702 1496 1576
Fragments arranged by size:
1576 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1211 1732 2410 2684 2831 3242 1211
Size: 521 678 274 147 411 1991
Fragments arranged by size:
1991 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 313
Size: 4022
BseYI C'CCAG_C
Cuts at: 411 1447 2205 2782 3278 411
Size: 1036 758 577 496 1155
Fragments arranged by size:
1155 1036 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 462 302
Size: 160 3862
BsiEI CG_ry'CG
Cuts at: 104 1564 1610 2818 104
Size: 1460 46 1208 1308
Fragments arranged by size:
1460 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 499 879 1036 1814 2004 2796 499
Size: 380 157 778 190 792 1725
Fragments arranged by size:
1725 792 778 380 190 157
Bsp1286I G_dGCh'C
Cuts at: 499 511 879 1036 1647 1740 1814 2004 2066
Size: 12 368 157 611 93 74 190 62
Cuts at: 2066 2796 499
Size: 730 1725
Fragments arranged by size:
1725 730 611 368 190 157 93 74
62 12
BspEI T'CCGG_A
Cuts at: 3242 3242
Size: 4022
BspHI T'CATG_A
Cuts at: 3198 3198
Size: 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1072 1286 1588 1969 1072
Size: 214 302 381 3125
Fragments arranged by size:
3125 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2314 2368 3852 4017 50
Size: 2264 54 1484 165 55
Fragments arranged by size:
2264 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 453 897 1934 453
Size: 444 1037 2541
Fragments arranged by size:
2541 1037 444
BsrFI r'CCGG_y
Cuts at: 967 2020 2201 2410 967
Size: 1053 181 209 2579
Fragments arranged by size:
2579 1053 209 181
BsrGI T'GTAC_A
Cuts at: 785 798 785
Size: 13 4009
BssSI C'ACGA_G
Cuts at: 2293 2651 2293
Size: 358 3664
BstAPI GCAn_nnn'nTGC
Cuts at: 353 687 1399 353
Size: 334 712 2976
Fragments arranged by size:
2976 712 334
BstBI TT'CG_AA
Cuts at: 2383 2383
Size: 4022
BstEII G'GTnAC_C
Cuts at: 633 633
Size: 4022
BstYI r'GATC_y
Cuts at: 1 119 701 1005 1047 1514 1537 1872 2118
Size: 118 582 304 42 467 23 335 246
Cuts at: 2118 3130 3216 3228 3574 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 582 467 449 346 335 304 246
118 86 42 23 12
BtgI C'CryG_G
Cuts at: 743 890 743
Size: 147 3875
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 933 1972 2153 2316 933
Size: 1039 181 163 2639
Fragments arranged by size:
2639 1039 181 163
BtsI GCAGTG_nn'
Cuts at: 333 868 1265 333
Size: 535 397 3090
Fragments arranged by size:
3090 535 397
DraI TTT'AAA
Cuts at: 1021 1021
Size: 4022
DraIII CAC_nnn'GTG
Cuts at: 494 744 874 494
Size: 250 130 3642
Fragments arranged by size:
3642 250 130
DrdI GACnn_nn'nnGTC
Cuts at: 1728 2586 1728
Size: 858 3164
EaeI y'GGCC_r
Cuts at: 140 813 1607 1781 2172 2199 3544 140
Size: 673 794 174 391 27 1345 618
Fragments arranged by size:
1345 794 673 618 391 174 27
EagI C'GGCC_G
Cuts at: 1607 1607
Size: 4022
EarI CTCTTCn'nnn_
Cuts at: 290 474 2045 2255 290
Size: 184 1571 210 2057
Fragments arranged by size:
2057 1571 210 184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 325 2230 2540 2686 3832 325
Size: 1905 310 146 1146 515
Fragments arranged by size:
1905 1146 515 310 146
EcoICRI GAG'CTC
Cuts at: 1034 1034
Size: 4022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 745 1847 2279 3026 3294 523
Size: 222 1102 432 747 268 1251
Fragments arranged by size:
1251 1102 747 432 268 222
EcoO109I rG'GnC_Cy
Cuts at: 113 149 396 1161 113
Size: 36 247 765 2974
Fragments arranged by size:
2974 765 247 36
EcoRI G'AATT_C
Cuts at: 592 1026 592
Size: 434 3588
EcoRV GAT'ATC
Cuts at: 996 996
Size: 4022
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 4022
FspI TGC'GCA
Cuts at: 604 1803 604
Size: 1199 2823
HaeII r_GCGC'y
Cuts at: 723 1456 1704 2726 3705 723
Size: 733 248 1022 979 1040
Fragments arranged by size:
1040 1022 979 733 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1864 1894 1896 1926 1864
Size: 30 2 30 3960
Fragments arranged by size:
3960 30 30 2
HincII GTy'rAC
Cuts at: 1061 1061
Size: 4022
KasI G'GCGC_C
Cuts at: 1452 1700 1452
Size: 248 3774
KpnI G_GTAC'C
Cuts at: 846 1042 846
Size: 196 3826
MluI A'CGCG_T
Cuts at: 3455 3455
Size: 4022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2693 2877 2693
Size: 184 3838
MscI TGG'CCA
Cuts at: 1783 1783
Size: 4022
MslI CAynn'nnrTG
Cuts at: 379 385 710 889 2011 2138 379
Size: 6 325 179 1122 127 2263
Fragments arranged by size:
2263 1122 325 179 127 6
MspA1I CmG'CkG
Cuts at: 407 819 1133 1447 1807 2820 3065 407
Size: 412 314 314 360 1013 245 1364
Fragments arranged by size:
1364 1013 412 360 314 314 245
NaeI GCC'GGC
Cuts at: 969 2203 969
Size: 1234 2788
NarI GG'CG_CC
Cuts at: 1453 1701 1453
Size: 248 3774
NcoI C'CATG_G
Cuts at: 890 890
Size: 4022
NgoMIV G'CCGG_C
Cuts at: 967 2201 967
Size: 1234 2788
NspI r_CATG'y
Cuts at: 609 786 1075 1575 2482 609
Size: 177 289 500 907 2149
Fragments arranged by size:
2149 907 500 289 177
PciI A'CATG_T
Cuts at: 782 2478 782
Size: 1696 2326
PflMI CCAn_nnn'nTGG
Cuts at: 744 744
Size: 4022
PmeI GTTT'AAAC
Cuts at: 1021 1021
Size: 4022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 3990
PpuMI rG'GwC_Cy
Cuts at: 396 396
Size: 4022
PstI C_TGCA'G
Cuts at: 1069 1754 1069
Size: 685 3337
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4022
PvuII CAG'CTG
Cuts at: 407 819 1447 1807 407
Size: 412 628 360 2622
Fragments arranged by size:
2622 628 412 360
RsrII CG'GwC_CG
Cuts at: 2217 2217
Size: 4022
SacI G_AGCT'C
Cuts at: 1036 1036
Size: 4022
SalI G'TCGA_C
Cuts at: 1059 1059
Size: 4022
SapI GCTCTTCn'nnn_
Cuts at: 2045 2255 2045
Size: 210 3812
SbfI CC_TGCA'GG
Cuts at: 1069 1069
Size: 4022
ScaI AGT'ACT
Cuts at: 3539 3539
Size: 4022
SfcI C'TryA_G
Cuts at: 33 1065 1750 2743 2934 33
Size: 1032 685 993 191 1121
Fragments arranged by size:
1121 1032 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 148 148
Size: 4022
SfoI GGC'GCC
Cuts at: 1454 1702 1454
Size: 248 3774
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
SmaI CCC'GGG
Cuts at: 760 1044 760
Size: 284 3738
SmlI C'TyrA_G
Cuts at: 619 1177 2092 2584 2846 619
Size: 558 915 492 262 1795
Fragments arranged by size:
1795 915 558 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4022
SphI G_CATG'C
Cuts at: 609 1075 1575 609
Size: 466 500 3056
Fragments arranged by size:
3056 500 466
StyI C'CwwG_G
Cuts at: 152 890 1156 1510 152
Size: 738 266 354 2664
Fragments arranged by size:
2664 738 354 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 604 1605 1738 2197 2406 604
Size: 1001 133 459 209 2220
Fragments arranged by size:
2220 1001 459 209 133
TatI w'GTAC_w
Cuts at: 785 798 944 3537 785
Size: 13 146 2593 1270
Fragments arranged by size:
2593 1270 146 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3539 3571 3539
Size: 32 3990
Tth111I GACn'n_nGTC
Cuts at: 1819 1819
Size: 4022
XbaI T'CTAG_A
Cuts at: 1053 1053
Size: 4022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1517 1517
Size: 4022
XmaI C'CCGG_G
Cuts at: 758 1042 758
Size: 284 3738
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BcgI BfrBI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI