Restriction map for
pF1KB4600
(Circular) (Six-base) MAPSORT of: pF1KB4600.seq Check: 3873 from: 1 to: 4031
pF1KB4600 4031 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 382 382
Size: 4031
AccI GT'mk_AC
Cuts at: 1069 1069
Size: 4031
Acc65I G'GTAC_C
Cuts at: 1047 1047
Size: 4031
AclI AA'CG_TT
Cuts at: 119 3871 119
Size: 3752 279
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 1856 2288 3035 3303 670
Size: 1186 432 747 268 1398
Fragments arranged by size:
1398 1186 747 432 268
AflIII A'CryG_T
Cuts at: 531 1383 2487 3464 531
Size: 852 1104 977 1098
Fragments arranged by size:
1104 1098 977 852
AgeI A'CCGG_T
Cuts at: 2419 2419
Size: 4031
AleI CACnn'nnGTG
Cuts at: 184 184
Size: 4031
AlwNI CAG_nnn'CTG
Cuts at: 1336 2903 1336
Size: 1567 2464
ApaLI G'TGCA_C
Cuts at: 2801 2801
Size: 4031
ApoI r'AATT_y
Cuts at: 1035 3384 3640 1035
Size: 2349 256 1426
Fragments arranged by size:
2349 1426 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4031
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
AvaI C'yCGr_G
Cuts at: 296 557 1051 296
Size: 261 494 3276
Fragments arranged by size:
3276 494 261
BamHI G'GATC_C
Cuts at: 1056 1056
Size: 4031
BanI G'GyrC_C
Cuts at: 1047 1461 1709 1744 1047
Size: 414 248 35 3334
Fragments arranged by size:
3334 414 248 35
BanII G_rGCy'C
Cuts at: 1045 2075 1045
Size: 1030 3001
BbeI G_GCGC'C
Cuts at: 1465 1713 1465
Size: 248 3783
BbvCI CC'TCA_GC
Cuts at: 890 977 890
Size: 87 3944
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 200 234 200
Size: 34 3997
BciVI GTATCCnnnnn_n'
Cuts at: 1929 2690 4031 1929
Size: 761 1341 1929
Fragments arranged by size:
1929 1341 761
BclI T'GATC_A
Cuts at: 953 1551 953
Size: 598 3433
BglII A'GATC_T
Cuts at: 1546 3583 1546
Size: 2037 1994
BlpI GC'TnA_GC
Cuts at: 1143 1143
Size: 4031
Bme1580I G_kGCm'C
Cuts at: 572 1656 1749 2805 572
Size: 1084 93 1056 1798
Fragments arranged by size:
1798 1084 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1243 1324 1659 3803 1243
Size: 81 335 2144 1471
Fragments arranged by size:
2144 1471 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1038 2398 1038
Size: 1360 2671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 967
Size: 4031
Bpu10I CC'TnA_GC
Cuts at: 890 977 890
Size: 87 3944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 2086 2578 2876 1207
Size: 879 492 298 2362
Fragments arranged by size:
2362 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3735 3735
Size: 4031
BsaAI yAC'GTr
Cuts at: 532 1384 532
Size: 852 3179
BsaBI GATnn'nnATC
Cuts at: 6 1545 3582 6
Size: 1539 2037 455
Fragments arranged by size:
2037 1539 455
BsaHI Gr'CG_yC
Cuts at: 1462 1710 2412 3908 1462
Size: 248 702 1496 1585
Fragments arranged by size:
1585 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1220 1741 2419 2693 2840 3251 1220
Size: 521 678 274 147 411 2000
Fragments arranged by size:
2000 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 456 530 533 536 826 907 456
Size: 74 3 3 290 81 3580
Fragments arranged by size:
3580 290 81 74 3 3
BseYI C'CCAG_C
Cuts at: 525 1456 2214 2791 3287 525
Size: 931 758 577 496 1269
Fragments arranged by size:
1269 931 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 896 896
Size: 4031
BsiEI CG_ry'CG
Cuts at: 104 1573 1619 2827 104
Size: 1469 46 1208 1308
Fragments arranged by size:
1469 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1045 1823 2013 2805 1045
Size: 778 190 792 2271
Fragments arranged by size:
2271 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 572 1045 1656 1749 1823 2013 2075 2805 572
Size: 473 611 93 74 190 62 730 1798
Fragments arranged by size:
1798 730 611 473 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3251 3251
Size: 4031
BspHI T'CATG_A
Cuts at: 3207 3207
Size: 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 382 1081 1295 1597 1978 382
Size: 699 214 302 381 2435
Fragments arranged by size:
2435 699 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2323 2377 3861 4026 50
Size: 2273 54 1484 165 55
Fragments arranged by size:
2273 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1943 1943
Size: 4031
BsrFI r'CCGG_y
Cuts at: 2029 2210 2419 2029
Size: 181 209 3641
Fragments arranged by size:
3641 209 181
BsrGI T'GTAC_A
Cuts at: 515 515
Size: 4031
BssSI C'ACGA_G
Cuts at: 2302 2660 2302
Size: 358 3673
BstAPI GCAn_nnn'nTGC
Cuts at: 1408 1408
Size: 4031
BstBI TT'CG_AA
Cuts at: 220 2392 220
Size: 2172 1859
BstYI r'GATC_y
Cuts at: 1 135 649 1056 1523 1546 1881 2127 3139
Size: 134 514 407 467 23 335 246 1012
Cuts at: 3139 3225 3237 3583 1
Size: 86 12 346 449
Fragments arranged by size:
1012 514 467 449 407 346 335 246
134 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4031
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1981 2162 2325 1981
Size: 181 163 3687
Fragments arranged by size:
3687 181 163
BtsI GCAGTG_nn'
Cuts at: 1274 1274
Size: 4031
DraI TTT'AAA
Cuts at: 1030 1030
Size: 4031
DrdI GACnn_nn'nnGTC
Cuts at: 1737 2595 1737
Size: 858 3173
EaeI y'GGCC_r
Cuts at: 110 336 1616 1790 2181 2208 3553 110
Size: 226 1280 174 391 27 1345 588
Fragments arranged by size:
1345 1280 588 391 226 174 27
EagI C'GGCC_G
Cuts at: 1616 1616
Size: 4031
EarI CTCTTCn'nnn_
Cuts at: 803 2054 2264 803
Size: 1251 210 2570
Fragments arranged by size:
2570 1251 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2239 2549 2695 3841 2239
Size: 310 146 1146 2429
Fragments arranged by size:
2429 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1043 1043
Size: 4031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 967 1856 2288 3035 3303 670
Size: 297 889 432 747 268 1398
Fragments arranged by size:
1398 889 747 432 297 268
EcoO109I rG'GnC_Cy
Cuts at: 900 1170 900
Size: 270 3761
EcoRI G'AATT_C
Cuts at: 1035 1035
Size: 4031
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2050 2050
Size: 4031
FspI TGC'GCA
Cuts at: 1812 1812
Size: 4031
HaeII r_GCGC'y
Cuts at: 1007 1465 1713 2735 3714 1007
Size: 458 248 1022 979 1324
Fragments arranged by size:
1324 1022 979 458 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 123 127 155 159 1873 1903 1905 1935 123
Size: 4 28 4 1714 30 2 30 2219
Fragments arranged by size:
2219 1714 30 30 28 4 4 2
HincII GTy'rAC
Cuts at: 1070 1070
Size: 4031
KasI G'GCGC_C
Cuts at: 1461 1709 1461
Size: 248 3783
KpnI G_GTAC'C
Cuts at: 1051 1051
Size: 4031
MluI A'CGCG_T
Cuts at: 3464 3464
Size: 4031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2702 2886 2702
Size: 184 3847
MscI TGG'CCA
Cuts at: 112 338 1792 112
Size: 226 1454 2351
Fragments arranged by size:
2351 1454 226
MslI CAynn'nnrTG
Cuts at: 184 2020 2147 184
Size: 1836 127 2068
Fragments arranged by size:
2068 1836 127
MspA1I CmG'CkG
Cuts at: 412 645 945 1142 1456 1816 2829 3074 412
Size: 233 300 197 314 360 1013 245 1369
Fragments arranged by size:
1369 1013 360 314 300 245 233 197
NaeI GCC'GGC
Cuts at: 2212 2212
Size: 4031
NarI GG'CG_CC
Cuts at: 1462 1710 1462
Size: 248 3783
NcoI C'CATG_G
Cuts at: 107 107
Size: 4031
NgoMIV G'CCGG_C
Cuts at: 2210 2210
Size: 4031
NspI r_CATG'y
Cuts at: 1084 1584 2491 1084
Size: 500 907 2624
Fragments arranged by size:
2624 907 500
PciI A'CATG_T
Cuts at: 2487 2487
Size: 4031
PfoI T'CCnGG_A
Cuts at: 737 737
Size: 4031
PmeI GTTT'AAAC
Cuts at: 1030 1030
Size: 4031
PmlI CAC'GTG
Cuts at: 532 532
Size: 4031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 3999
PpuMI rG'GwC_Cy
Cuts at: 900 900
Size: 4031
PstI C_TGCA'G
Cuts at: 366 411 1078 1763 366
Size: 45 667 685 2634
Fragments arranged by size:
2634 685 667 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4031
PvuII CAG'CTG
Cuts at: 945 1456 1816 945
Size: 511 360 3160
Fragments arranged by size:
3160 511 360
RsrII CG'GwC_CG
Cuts at: 2226 2226
Size: 4031
SacI G_AGCT'C
Cuts at: 1045 1045
Size: 4031
SalI G'TCGA_C
Cuts at: 1068 1068
Size: 4031
SapI GCTCTTCn'nnn_
Cuts at: 2054 2264 2054
Size: 210 3821
SbfI CC_TGCA'GG
Cuts at: 1078 1078
Size: 4031
ScaI AGT'ACT
Cuts at: 3548 3548
Size: 4031
SfcI C'TryA_G
Cuts at: 33 305 362 407 563 1074 1759 2752 2943
Size: 272 57 45 156 511 685 993 191
Cuts at: 2943 33
Size: 1121
Fragments arranged by size:
1121 993 685 511 272 191 156 57
45
SfoI GGC'GCC
Cuts at: 1463 1711 1463
Size: 248 3783
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
SmaI CCC'GGG
Cuts at: 298 1053 298
Size: 755 3276
SmlI C'TyrA_G
Cuts at: 1186 2101 2593 2855 1186
Size: 915 492 262 2362
Fragments arranged by size:
2362 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4031
SphI G_CATG'C
Cuts at: 1084 1584 1084
Size: 500 3531
SrfI GCCC'GGGC
Cuts at: 298 298
Size: 4031
StyI C'CwwG_G
Cuts at: 107 1165 1519 107
Size: 1058 354 2619
Fragments arranged by size:
2619 1058 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1614 1747 2206 2415 1614
Size: 133 459 209 3230
Fragments arranged by size:
3230 459 209 133
TatI w'GTAC_w
Cuts at: 515 3546 515
Size: 3031 1000
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3548 3580 3548
Size: 32 3999
Tth111I GACn'n_nGTC
Cuts at: 1828 1828
Size: 4031
XbaI T'CTAG_A
Cuts at: 1062 1062
Size: 4031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1526 1526
Size: 4031
XmaI C'CCGG_G
Cuts at: 296 1051 296
Size: 755 3276
XmnI GAAnn'nnTTC
Cuts at: 600 600
Size: 4031
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SspI StuI SwaI
XhoI ZraI