Restriction map for
pF1KB4596
(Circular) (Six-base) MAPSORT of: pF1KB4596.seq Check: 7991 from: 1 to: 5864
pF1KB4596 5864 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2551 2551
Size: 5864
AccI GT'mk_AC
Cuts at: 1343 2902 1343
Size: 1559 4305
Acc65I G'GTAC_C
Cuts at: 2880 2880
Size: 5864
AclI AA'CG_TT
Cuts at: 5704 5704
Size: 5864
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 219 2455 3689 4121 4474 5136 169
Size: 50 2236 1234 432 353 662 897
Fragments arranged by size:
2236 1234 897 662 432 353 50
AflIII A'CryG_T
Cuts at: 2768 3216 5016 5297 2768
Size: 448 1800 281 3335
Fragments arranged by size:
3335 1800 448 281
AleI CACnn'nnGTG
Cuts at: 470 2604 2824 470
Size: 2134 220 3510
Fragments arranged by size:
3510 2134 220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1640 2004 1640
Size: 364 5500
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1007 1039 1007
Size: 32 5832
AlwNI CAG_nnn'CTG
Cuts at: 126 3169 4607 126
Size: 3043 1438 1383
Fragments arranged by size:
3043 1438 1383
ApaLI G'TGCA_C
Cuts at: 463 4702 463
Size: 4239 1625
ApoI r'AATT_y
Cuts at: 380 2044 2868 5217 5473 380
Size: 1664 824 2349 256 771
Fragments arranged by size:
2349 1664 824 771 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5864
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5864
AvaI C'yCGr_G
Cuts at: 263 1097 2441 2884 263
Size: 834 1344 443 3243
Fragments arranged by size:
3243 1344 834 443
BamHI G'GATC_C
Cuts at: 958 2889 958
Size: 1931 3933
BanI G'GyrC_C
Cuts at: 742 805 1279 1410 2086 2880 3294 3542 3577
Size: 63 474 131 676 794 414 248 35
Cuts at: 3577 742
Size: 3029
Fragments arranged by size:
3029 794 676 474 414 248 131 63
35
BanII G_rGCy'C
Cuts at: 2117 2878 3908 2117
Size: 761 1030 4073
Fragments arranged by size:
4073 1030 761
BbeI G_GCGC'C
Cuts at: 1283 3298 3546 1283
Size: 2015 248 3601
Fragments arranged by size:
3601 2015 248
BbsI GAAGACnn'nnnn_
Cuts at: 362 926 2443 362
Size: 564 1517 3783
Fragments arranged by size:
3783 1517 564
BbvCI CC'TCA_GC
Cuts at: 1603 1603
Size: 5864
BciVI GTATCCnnnnn_n'
Cuts at: 2566 3762 4818 5864 2566
Size: 1196 1056 1046 2566
Fragments arranged by size:
2566 1196 1056 1046
BclI T'GATC_A
Cuts at: 489 611 791 1833 1955 2339 3384 489
Size: 122 180 1042 122 384 1045 2969
Fragments arranged by size:
2969 1045 1042 384 180 122 122
BglII A'GATC_T
Cuts at: 1781 2420 3379 5416 1781
Size: 639 959 2037 2229
Fragments arranged by size:
2229 2037 959 639
BlpI GC'TnA_GC
Cuts at: 1251 2976 1251
Size: 1725 4139
Bme1580I G_kGCm'C
Cuts at: 467 745 1315 1816 2089 3489 3582 4706 467
Size: 278 570 501 273 1400 93 1124 1625
Fragments arranged by size:
1625 1400 1124 570 501 278 273 93
BmgBI CAC'GTC
Cuts at: 2058 2058
Size: 5864
BmrI ACTGGGnnnn_n'
Cuts at: 1285 1826 3076 3157 3492 5636 1285
Size: 541 1250 81 335 2144 1513
Fragments arranged by size:
2144 1513 1250 541 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4231 4231
Size: 5864
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1582 2485 2503 2706 1582
Size: 903 18 203 4740
Fragments arranged by size:
4740 903 203 18
Bpu10I CC'TnA_GC
Cuts at: 1603 1603
Size: 5864
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3040 3919 4633 4931 3040
Size: 879 714 298 3973
Fragments arranged by size:
3973 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1516 2449 5568 1516
Size: 933 3119 1812
Fragments arranged by size:
3119 1812 933
BsaAI yAC'GTr
Cuts at: 1961 3217 1961
Size: 1256 4608
BsaBI GATnn'nnATC
Cuts at: 6 272 874 2218 3378 5415 6
Size: 266 602 1344 1160 2037 455
Fragments arranged by size:
2037 1344 1160 602 455 266
BsaHI Gr'CG_yC
Cuts at: 1280 3295 3543 4245 5741 1280
Size: 2015 248 702 1496 1403
Fragments arranged by size:
2015 1496 1403 702 248
BsaWI w'CCGG_w
Cuts at: 926 1655 3053 3574 4252 4663 4810 5084 926
Size: 729 1398 521 678 411 147 274 1706
Fragments arranged by size:
1706 1398 729 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 644 674 858 888 1007 1037 1828 1858 644
Size: 30 184 30 119 30 791 30 4650
Fragments arranged by size:
4650 791 184 119 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1426 1534 2125 2185 1426
Size: 108 591 60 5105
Fragments arranged by size:
5105 591 108 60
BseYI C'CCAG_C
Cuts at: 1596 2314 3289 4047 4712 5120 1596
Size: 718 975 758 665 408 2340
Fragments arranged by size:
2340 975 758 718 665 408
BsiEI CG_ry'CG
Cuts at: 104 2825 3406 3452 4682 104
Size: 2721 581 46 1230 1286
Fragments arranged by size:
2721 1286 1230 581 46
BsiHKAI G_wGCw'C
Cuts at: 467 2878 3656 3846 4706 467
Size: 2411 778 190 860 1625
Fragments arranged by size:
2411 1625 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 851 896 2422 851
Size: 45 1526 4293
Fragments arranged by size:
4293 1526 45
Bsp1286I G_dGCh'C
Cuts at: 467 745 1315 1816 2089 2117 2878 3489 3582
Size: 278 570 501 273 28 761 611 93
Cuts at: 3582 3656 3846 3908 4706 467
Size: 74 190 62 798 1625
Fragments arranged by size:
1625 798 761 611 570 501 278 273
190 93 74 62 28
BspEI T'CCGG_A
Cuts at: 926 1655 926
Size: 729 5135
BspHI T'CATG_A
Cuts at: 224 402 4296 224
Size: 178 3894 1792
Fragments arranged by size:
3894 1792 178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2551 2914 3128 3430 3811 2551
Size: 363 214 302 381 4604
Fragments arranged by size:
4604 381 363 302 214
BsrBI CCG'CTC
Cuts at: 50 641 4156 4210 5694 5859 50
Size: 591 3515 54 1484 165 55
Fragments arranged by size:
3515 1484 591 165 55 54
BsrDI GCAATG_nn'
Cuts at: 347 2160 3776 347
Size: 1813 1616 2435
Fragments arranged by size:
2435 1813 1616
BsrFI r'CCGG_y
Cuts at: 1508 2146 3862 4043 1508
Size: 638 1716 181 3329
Fragments arranged by size:
3329 1716 638 181
BssSI C'ACGA_G
Cuts at: 4135 4843 4135
Size: 708 5156
BstAPI GCAn_nnn'nTGC
Cuts at: 2159 3241 2159
Size: 1082 4782
BstBI TT'CG_AA
Cuts at: 378 4225 378
Size: 3847 2017
BstXI CCAn_nnnn'nTGG
Cuts at: 1263 1263
Size: 5864
BstYI r'GATC_y
Cuts at: 1 357 958 1348 1701 1781 2420 2639 2889
Size: 356 601 390 353 80 639 219 250
Cuts at: 2889 3356 3379 3714 3960 4266 4278 4364 5416
Size: 467 23 335 246 306 12 86 1052
Cuts at: 5416 1
Size: 449
Fragments arranged by size:
1052 639 601 467 449 390 356 353
335 306 250 246 219 86 80 23
12
BtgI C'CryG_G
Cuts at: 1331 1469 1628 2825 1331
Size: 138 159 1197 4370
Fragments arranged by size:
4370 1197 159 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2561 3814 3995 4158 2561
Size: 1253 181 163 4267
Fragments arranged by size:
4267 1253 181 163
BtsI GCAGTG_nn'
Cuts at: 477 2494 3107 477
Size: 2017 613 3234
Fragments arranged by size:
3234 2017 613
DraI TTT'AAA
Cuts at: 633 2863 633
Size: 2230 3634
DrdI GACnn_nn'nnGTC
Cuts at: 2663 3570 4914 2663
Size: 907 1344 3613
Fragments arranged by size:
3613 1344 907
EaeI y'GGCC_r
Cuts at: 1046 1496 2822 3449 3623 4014 4041 5386 1046
Size: 450 1326 627 174 391 27 1345 1524
Fragments arranged by size:
1524 1345 1326 627 450 391 174 27
EagI C'GGCC_G
Cuts at: 2822 3449 2822
Size: 627 5237
EarI CTCTTCn'nnn_
Cuts at: 1567 1817 2413 3887 4097 1567
Size: 250 596 1474 210 3334
Fragments arranged by size:
3334 1474 596 250 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1089 4072 4814 4960 5674 1089
Size: 2983 742 146 714 1279
Fragments arranged by size:
2983 1279 742 714 146
EcoICRI GAG'CTC
Cuts at: 2876 2876
Size: 5864
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 219 1582 2455 2485 2503 2706 3689 4121
Size: 50 1363 873 30 18 203 983 432
Cuts at: 4121 4474 5136 169
Size: 353 662 897
Fragments arranged by size:
1363 983 897 873 662 432 353 203
50 30 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2016 2785 2016
Size: 769 5095
EcoO109I rG'GnC_Cy
Cuts at: 1056 1885 2237 3003 1056
Size: 829 352 766 3917
Fragments arranged by size:
3917 829 766 352
EcoRI G'AATT_C
Cuts at: 380 2868 380
Size: 2488 3376
EcoRV GAT'ATC
Cuts at: 222 2268 222
Size: 2046 3818
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3883 3883
Size: 5864
FspI TGC'GCA
Cuts at: 3645 3645
Size: 5864
HaeII r_GCGC'y
Cuts at: 1283 1407 3298 3546 4776 5547 1283
Size: 124 1891 248 1230 771 1600
Fragments arranged by size:
1891 1600 1230 771 248 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 895 927 943 975 1198 1230 1515 1547 2405
Size: 32 16 32 223 32 285 32 858
Cuts at: 2405 2437 3706 3736 3738 3768 895
Size: 32 1269 30 2 30 2991
Fragments arranged by size:
2991 1269 858 285 223 32 32 32
32 32 30 30 16 2
HincII GTy'rAC
Cuts at: 1344 2073 2903 1344
Size: 729 830 4305
Fragments arranged by size:
4305 830 729
HindIII A'AGCT_T
Cuts at: 2704 2704
Size: 5864
KasI G'GCGC_C
Cuts at: 1279 3294 3542 1279
Size: 2015 248 3601
Fragments arranged by size:
3601 2015 248
KpnI G_GTAC'C
Cuts at: 2884 2884
Size: 5864
MluI A'CGCG_T
Cuts at: 5297 5297
Size: 5864
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 301 2109 4623 4807 301
Size: 1808 2514 184 1358
Fragments arranged by size:
2514 1808 1358 184
MscI TGG'CCA
Cuts at: 1048 3625 1048
Size: 2577 3287
MslI CAynn'nnrTG
Cuts at: 470 748 1622 2092 2224 2604 2760 2824 3853
Size: 278 874 470 132 380 156 64 1029
Cuts at: 3853 3980 470
Size: 127 2354
Fragments arranged by size:
2354 1029 874 470 380 278 156 132
127 64
MspA1I CmG'CkG
Cuts at: 981 1454 2604 2798 2975 3289 3649 4433 4678
Size: 473 1150 194 177 314 360 784 245
Cuts at: 4678 981
Size: 2167
Fragments arranged by size:
2167 1150 784 473 360 314 245 194
177
NaeI GCC'GGC
Cuts at: 4045 4045
Size: 5864
NarI GG'CG_CC
Cuts at: 1280 3295 3543 1280
Size: 2015 248 3601
Fragments arranged by size:
3601 2015 248
NcoI C'CATG_G
Cuts at: 1469 1469
Size: 5864
NgoMIV G'CCGG_C
Cuts at: 4043 4043
Size: 5864
NspI r_CATG'y
Cuts at: 1008 1557 2917 3417 5020 1008
Size: 549 1360 500 1603 1852
Fragments arranged by size:
1852 1603 1360 549 500
PciI A'CATG_T
Cuts at: 5016 5016
Size: 5864
PfoI T'CCnGG_A
Cuts at: 2587 2587
Size: 5864
PmeI GTTT'AAAC
Cuts at: 2863 2863
Size: 5864
PmlI CAC'GTG
Cuts at: 1961 1961
Size: 5864
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1007 1039 4310 4342 1007
Size: 32 3271 32 2529
Fragments arranged by size:
3271 2529 32 32
PstI C_TGCA'G
Cuts at: 2911 3596 2911
Size: 685 5179
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5864
PvuII CAG'CTG
Cuts at: 981 2604 3289 3649 981
Size: 1623 685 360 3196
Fragments arranged by size:
3196 1623 685 360
RsrII CG'GwC_CG
Cuts at: 4059 4059
Size: 5864
SacI G_AGCT'C
Cuts at: 2878 2878
Size: 5864
SalI G'TCGA_C
Cuts at: 1342 2901 1342
Size: 1559 4305
SapI GCTCTTCn'nnn_
Cuts at: 1817 3887 4097 1817
Size: 2070 210 3584
Fragments arranged by size:
3584 2070 210
SbfI CC_TGCA'GG
Cuts at: 2911 2911
Size: 5864
ScaI AGT'ACT
Cuts at: 5381 5381
Size: 5864
SexAI A'CCwGG_T
Cuts at: 158 158
Size: 5864
SfcI C'TryA_G
Cuts at: 33 2907 3592 4560 4751 33
Size: 2874 685 968 191 1146
Fragments arranged by size:
2874 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1281 3296 3544 1281
Size: 2015 248 3601
Fragments arranged by size:
3601 2015 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5864
SmaI CCC'GGG
Cuts at: 265 2443 2886 265
Size: 2178 443 3243
Fragments arranged by size:
3243 2178 443
SmlI C'TyrA_G
Cuts at: 3019 3934 4648 4910 3019
Size: 915 714 262 3973
Fragments arranged by size:
3973 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5864
SphI G_CATG'C
Cuts at: 2917 3417 2917
Size: 500 5364
SspI AAT'ATT
Cuts at: 2302 2302
Size: 5864
StuI AGG'CCT
Cuts at: 1151 1151
Size: 5864
StyI C'CwwG_G
Cuts at: 1049 1469 2998 3352 1049
Size: 420 1529 354 3561
Fragments arranged by size:
3561 1529 420 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1332 1382 1690 2347 2501 3447 3580 4039 4248
Size: 50 308 657 154 946 133 459 209
Cuts at: 4248 1332
Size: 2948
Fragments arranged by size:
2948 946 657 459 308 209 154 133
50
TatI w'GTAC_w
Cuts at: 1007 5379 1007
Size: 4372 1492
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 415 447 644 676 1828 1860 2239 2271 5381
Size: 32 197 32 1152 32 379 32 3110
Cuts at: 5381 5413 415
Size: 32 866
Fragments arranged by size:
3110 1152 866 379 197 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 160 1210 3661 160
Size: 1050 2451 2363
Fragments arranged by size:
2451 2363 1050
XbaI T'CTAG_A
Cuts at: 2895 2895
Size: 5864
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1896 3359 1896
Size: 1463 4401
XmaI C'CCGG_G
Cuts at: 263 2441 2884 263
Size: 2178 443 3243
Fragments arranged by size:
3243 2178 443
XmnI GAAnn'nnTTC
Cuts at: 2401 2401
Size: 5864
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PmlI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmtI BsgI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI