Restriction map for
pF1KB4585
(Circular) (Six-base) MAPSORT of: pF1KB4585.seq Check: 7267 from: 1 to: 4151
pF1KB4585 4151 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 415 415
Size: 4151
AccI GT'mk_AC
Cuts at: 1091 1189 1091
Size: 98 4053
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 1976 2408 2761 3423 916
Size: 1060 432 353 662 1644
Fragments arranged by size:
1644 1060 662 432 353
AflIII A'CryG_T
Cuts at: 395 1503 3303 3584 395
Size: 1108 1800 281 962
Fragments arranged by size:
1800 1108 962 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 383 383
Size: 4151
AlwNI CAG_nnn'CTG
Cuts at: 1456 2894 1456
Size: 1438 2713
ApaLI G'TGCA_C
Cuts at: 2989 2989
Size: 4151
ApoI r'AATT_y
Cuts at: 1155 3504 3760 1155
Size: 2349 256 1546
Fragments arranged by size:
2349 1546 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 302 1171 302
Size: 869 3282
BamHI G'GATC_C
Cuts at: 1176 1176
Size: 4151
BanI G'GyrC_C
Cuts at: 841 911 994 1082 1167 1581 1829 1864 841
Size: 70 83 88 85 414 248 35 3128
Fragments arranged by size:
3128 414 248 88 85 83 70 35
BanII G_rGCy'C
Cuts at: 209 223 709 1004 1123 1165 2195 209
Size: 14 486 295 119 42 1030 2165
Fragments arranged by size:
2165 1030 486 295 119 42 14
BbeI G_GCGC'C
Cuts at: 1585 1833 1585
Size: 248 3903
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 366 400 366
Size: 34 4117
BciVI GTATCCnnnnn_n'
Cuts at: 222 2049 3105 4151 222
Size: 1827 1056 1046 222
Fragments arranged by size:
1827 1056 1046 222
BclI T'GATC_A
Cuts at: 1671 1671
Size: 4151
BglI GCCn_nnn'nGGC
Cuts at: 688 688
Size: 4151
BglII A'GATC_T
Cuts at: 1666 3703 1666
Size: 2037 2114
BlpI GC'TnA_GC
Cuts at: 1263 1263
Size: 4151
Bme1580I G_kGCm'C
Cuts at: 997 1085 1776 1869 2993 997
Size: 88 691 93 1124 2155
Fragments arranged by size:
2155 1124 691 93 88
BmrI ACTGGGnnnn_n'
Cuts at: 1363 1444 1779 3923 1363
Size: 81 335 2144 1591
Fragments arranged by size:
2144 1591 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 237 499 553 223
Size: 14 262 54 3821
Fragments arranged by size:
3821 262 54 14
Bpu10I CC'TnA_GC
Cuts at: 356 626 356
Size: 270 3881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1327 2206 2920 3218 1327
Size: 879 714 298 2260
Fragments arranged by size:
2260 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3855 3855
Size: 4151
BsaAI yAC'GTr
Cuts at: 1504 1504
Size: 4151
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 622 1582 1830 2532 4028 622
Size: 960 248 702 1496 745
Fragments arranged by size:
1496 960 745 702 248
BsaWI w'CCGG_w
Cuts at: 1340 1861 2539 2950 3097 3371 1340
Size: 521 678 411 147 274 2120
Fragments arranged by size:
2120 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 649 1003 1576 2334 2999 3407 649
Size: 354 573 758 665 408 1393
Fragments arranged by size:
1393 758 665 573 408 354
BsiEI CG_ry'CG
Cuts at: 104 1693 1739 2969 104
Size: 1589 46 1230 1286
Fragments arranged by size:
1589 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 223 1165 1943 2133 2993 223
Size: 942 778 190 860 1381
Fragments arranged by size:
1381 942 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 209 223 709 997 1004 1085 1123 1165 1776
Size: 14 486 288 7 81 38 42 611
Cuts at: 1776 1869 1943 2133 2195 2993 209
Size: 93 74 190 62 798 1367
Fragments arranged by size:
1367 798 611 486 288 190 93 81
74 62 42 38 14 7
BspHI T'CATG_A
Cuts at: 590 2583 590
Size: 1993 2158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 415 1201 1415 1717 2098 415
Size: 786 214 302 381 2468
Fragments arranged by size:
2468 786 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2443 2497 3981 4146 50
Size: 2393 54 1484 165 55
Fragments arranged by size:
2393 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2063 2063
Size: 4151
BsrFI r'CCGG_y
Cuts at: 173 844 2149 2330 173
Size: 671 1305 181 1994
Fragments arranged by size:
1994 1305 671 181
BssSI C'ACGA_G
Cuts at: 2422 3130 2422
Size: 708 3443
BstAPI GCAn_nnn'nTGC
Cuts at: 1528 1528
Size: 4151
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstEII G'GTnAC_C
Cuts at: 402 402
Size: 4151
BstXI CCAn_nnnn'nTGG
Cuts at: 189 201 399 471 189
Size: 12 198 72 3869
Fragments arranged by size:
3869 198 72 12
BstYI r'GATC_y
Cuts at: 1 606 1176 1643 1666 2001 2247 2553 2565
Size: 605 570 467 23 335 246 306 12
Cuts at: 2565 2651 3703 1
Size: 86 1052 449
Fragments arranged by size:
1052 605 570 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 166 2101 2282 2445 166
Size: 1935 181 163 1872
Fragments arranged by size:
1935 1872 181 163
BtsI GCAGTG_nn'
Cuts at: 987 1394 987
Size: 407 3744
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DrdI GACnn_nn'nnGTC
Cuts at: 670 1857 3201 670
Size: 1187 1344 1620
Fragments arranged by size:
1620 1344 1187
EaeI y'GGCC_r
Cuts at: 191 680 962 1736 1910 2301 2328 3673 191
Size: 489 282 774 174 391 27 1345 669
Fragments arranged by size:
1345 774 669 489 391 282 174 27
EagI C'GGCC_G
Cuts at: 1736 1736
Size: 4151
EarI CTCTTCn'nnn_
Cuts at: 746 751 2174 2384 746
Size: 5 1423 210 2513
Fragments arranged by size:
2513 1423 210 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2359 3101 3247 3961 2359
Size: 742 146 714 2549
Fragments arranged by size:
2549 742 714 146
EcoICRI GAG'CTC
Cuts at: 221 1163 221
Size: 942 3209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 237 499 553 916 1976 2408 2761 3423
Size: 14 262 54 363 1060 432 353 662
Cuts at: 3423 223
Size: 951
Fragments arranged by size:
1060 951 662 432 363 353 262 54
14
EcoNI CCTnn'n_nnAGG
Cuts at: 411 411
Size: 4151
EcoO109I rG'GnC_Cy
Cuts at: 690 792 1290 690
Size: 102 498 3551
Fragments arranged by size:
3551 498 102
EcoRI G'AATT_C
Cuts at: 1155 1155
Size: 4151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 4151
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 169 1585 1833 3063 3834 169
Size: 1416 248 1230 771 486
Fragments arranged by size:
1416 1230 771 486 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 196 228 1993 2023 2025 2055 196
Size: 32 1765 30 2 30 2292
Fragments arranged by size:
2292 1765 32 30 30 2
HincII GTy'rAC
Cuts at: 1190 1190
Size: 4151
KasI G'GCGC_C
Cuts at: 1581 1829 1581
Size: 248 3903
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2910 3094 2910
Size: 184 3967
MscI TGG'CCA
Cuts at: 193 682 964 1912 193
Size: 489 282 948 2432
Fragments arranged by size:
2432 948 489 282
MslI CAynn'nnrTG
Cuts at: 2140 2267 2140
Size: 127 4024
MspA1I CmG'CkG
Cuts at: 189 367 633 788 933 991 1262 1576 1936
Size: 178 266 155 145 58 271 314 360
Cuts at: 1936 2720 2965 189
Size: 784 245 1375
Fragments arranged by size:
1375 784 360 314 271 266 245 178
155 145 58
NaeI GCC'GGC
Cuts at: 175 2332 175
Size: 2157 1994
NarI GG'CG_CC
Cuts at: 1582 1830 1582
Size: 248 3903
NcoI C'CATG_G
Cuts at: 107 107
Size: 4151
NgoMIV G'CCGG_C
Cuts at: 173 2330 173
Size: 2157 1994
NspI r_CATG'y
Cuts at: 1204 1704 3307 1204
Size: 500 1603 2048
Fragments arranged by size:
2048 1603 500
PasI CC'CwG_GG
Cuts at: 711 711
Size: 4151
PciI A'CATG_T
Cuts at: 3303 3303
Size: 4151
PfoI T'CCnGG_A
Cuts at: 214 449 214
Size: 235 3916
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2597 2629 2597
Size: 32 4119
PpuMI rG'GwC_Cy
Cuts at: 792 792
Size: 4151
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1120 1152 1120
Size: 32 4119
PstI C_TGCA'G
Cuts at: 1198 1883 1198
Size: 685 3466
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 367 633 1576 1936 367
Size: 266 943 360 2582
Fragments arranged by size:
2582 943 360 266
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 223 1165 223
Size: 942 3209
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SanDI GG'GwC_CC
Cuts at: 792 792
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 2174 2384 2174
Size: 210 3941
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 331 3668 331
Size: 3337 814
SexAI A'CCwGG_T
Cuts at: 344 344
Size: 4151
SfcI C'TryA_G
Cuts at: 33 372 1031 1194 1879 2847 3038 33
Size: 339 659 163 685 968 191 1146
Fragments arranged by size:
1146 968 685 659 339 191 163
SfiI GGCCn_nnn'nGGCC
Cuts at: 688 688
Size: 4151
SfoI GGC'GCC
Cuts at: 1583 1831 1583
Size: 248 3903
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 1173 1173
Size: 4151
SmlI C'TyrA_G
Cuts at: 1306 2221 2935 3197 1306
Size: 915 714 262 2260
Fragments arranged by size:
2260 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 1204 1704 1204
Size: 500 3651
StyI C'CwwG_G
Cuts at: 107 815 971 1285 1639 107
Size: 708 156 314 354 2619
Fragments arranged by size:
2619 708 354 314 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 703 1734 1867 2326 2535 703
Size: 1031 133 459 209 2319
Fragments arranged by size:
2319 1031 459 209 133
TatI w'GTAC_w
Cuts at: 329 3666 329
Size: 3337 814
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3668 3700 3668
Size: 32 4119
Tth111I GACn'n_nGTC
Cuts at: 1948 1948
Size: 4151
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 528 1646 528
Size: 1118 3033
XmaI C'CCGG_G
Cuts at: 1171 1171
Size: 4151
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI
BsaXI BseRI BsgI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PshAI PsiI PspOMI PspXI
SacII SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI