Restriction map for
pF1KB4582
(Circular) (Six-base) MAPSORT of: pF1KB4582.seq Check: 1094 from: 1 to: 4997
pF1KB4582 4997 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 271 383 271
Size: 112 4885
AccI GT'mk_AC
Cuts at: 1446 2035 1446
Size: 589 4408
Acc65I G'GTAC_C
Cuts at: 2013 2013
Size: 4997
AclI AA'CG_TT
Cuts at: 4837 4837
Size: 4997
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 921 1798 2822 3254 3607 4269 784
Size: 137 877 1024 432 353 662 1512
Fragments arranged by size:
1512 1024 877 662 432 353 137
AflIII A'CryG_T
Cuts at: 2349 4149 4430 2349
Size: 1800 281 2916
Fragments arranged by size:
2916 1800 281
AleI CACnn'nnGTG
Cuts at: 151 151
Size: 4997
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1791 1823 1791
Size: 32 4965
AlwNI CAG_nnn'CTG
Cuts at: 792 1016 1100 2302 3740 792
Size: 224 84 1202 1438 2049
Fragments arranged by size:
2049 1438 1202 224 84
ApaLI G'TGCA_C
Cuts at: 442 1549 3835 442
Size: 1107 2286 1604
Fragments arranged by size:
2286 1604 1107
ApoI r'AATT_y
Cuts at: 225 478 2001 4350 4606 225
Size: 253 1523 2349 256 616
Fragments arranged by size:
2349 1523 616 256 253
AseI AT'TA_AT
Cuts at: 20 20
Size: 4997
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4997
AvaI C'yCGr_G
Cuts at: 2017 2017
Size: 4997
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1715 1748 1715
Size: 33 4964
BamHI G'GATC_C
Cuts at: 2022 2022
Size: 4997
BanI G'GyrC_C
Cuts at: 2013 2427 2675 2710 2013
Size: 414 248 35 4300
Fragments arranged by size:
4300 414 248 35
BanII G_rGCy'C
Cuts at: 1083 2011 3041 1083
Size: 928 1030 3039
Fragments arranged by size:
3039 1030 928
BbeI G_GCGC'C
Cuts at: 2431 2679 2431
Size: 248 4749
BciVI GTATCCnnnnn_n'
Cuts at: 1411 2895 3951 4997 1411
Size: 1484 1056 1046 1411
Fragments arranged by size:
1484 1411 1056 1046
BclI T'GATC_A
Cuts at: 912 2517 912
Size: 1605 3392
BfrBI ATG'CAT
Cuts at: 920 920
Size: 4997
BglII A'GATC_T
Cuts at: 2512 4549 2512
Size: 2037 2960
BlpI GC'TnA_GC
Cuts at: 2109 2109
Size: 4997
Bme1580I G_kGCm'C
Cuts at: 446 1032 1553 2622 2715 3839 446
Size: 586 521 1069 93 1124 1604
Fragments arranged by size:
1604 1124 1069 586 521 93
BmrI ACTGGGnnnn_n'
Cuts at: 1431 2209 2290 2625 4769 1431
Size: 778 81 335 2144 1659
Fragments arranged by size:
2144 1659 778 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3364 3364
Size: 4997
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 648 704 1256 200
Size: 448 56 552 3941
Fragments arranged by size:
3941 552 448 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2173 3052 3766 4064 2173
Size: 879 714 298 3106
Fragments arranged by size:
3106 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1251 4701 1251
Size: 3450 1547
BsaAI yAC'GTr
Cuts at: 2350 2350
Size: 4997
BsaBI GATnn'nnATC
Cuts at: 6 2511 4548 6
Size: 2505 2037 455
Fragments arranged by size:
2505 2037 455
BsaHI Gr'CG_yC
Cuts at: 2428 2676 3378 4874 2428
Size: 248 702 1496 2551
Fragments arranged by size:
2551 1496 702 248
BsaWI w'CCGG_w
Cuts at: 404 1483 2186 2707 3385 3796 3943 4217 404
Size: 1079 703 521 678 411 147 274 1184
Fragments arranged by size:
1184 1079 703 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 677 707 1065 1095 677
Size: 30 358 30 4579
Fragments arranged by size:
4579 358 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 701 701
Size: 4997
BseYI C'CCAG_C
Cuts at: 1351 2422 3180 3845 4253 1351
Size: 1071 758 665 408 2095
Fragments arranged by size:
2095 1071 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 129 805 1113 1586 129
Size: 676 308 473 3540
Fragments arranged by size:
3540 676 473 308
BsiEI CG_ry'CG
Cuts at: 104 2539 2585 3815 104
Size: 2435 46 1230 1286
Fragments arranged by size:
2435 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 446 1553 2011 2789 2979 3839 446
Size: 1107 458 778 190 860 1604
Fragments arranged by size:
1604 1107 860 778 458 190
BsmI GAATG_Cn'
Cuts at: 279 1660 279
Size: 1381 3616
Bsp1286I G_dGCh'C
Cuts at: 446 1032 1083 1553 2011 2622 2715 2789 2979
Size: 586 51 470 458 611 93 74 190
Cuts at: 2979 3041 3839 446
Size: 62 798 1604
Fragments arranged by size:
1604 798 611 586 470 458 190 93
74 62 51
BspHI T'CATG_A
Cuts at: 1524 1713 3429 1524
Size: 189 1716 3092
Fragments arranged by size:
3092 1716 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 271 383 1108 1259 2047 2261 2563 2944 271
Size: 112 725 151 788 214 302 381 2324
Fragments arranged by size:
2324 788 725 381 302 214 151 112
BsrBI CCG'CTC
Cuts at: 50 545 3289 3343 4827 4992 50
Size: 495 2744 54 1484 165 55
Fragments arranged by size:
2744 1484 495 165 55 54
BsrDI GCAATG_nn'
Cuts at: 354 906 1362 2909 354
Size: 552 456 1547 2442
Fragments arranged by size:
2442 1547 552 456
BsrFI r'CCGG_y
Cuts at: 522 1046 2995 3176 522
Size: 524 1949 181 2343
Fragments arranged by size:
2343 1949 524 181
BssHII G'CGCG_C
Cuts at: 119 119
Size: 4997
BssSI C'ACGA_G
Cuts at: 3268 3976 3268
Size: 708 4289
BstAPI GCAn_nnn'nTGC
Cuts at: 1016 1100 2374 1016
Size: 84 1274 3639
Fragments arranged by size:
3639 1274 84
BstBI TT'CG_AA
Cuts at: 3358 3358
Size: 4997
BstXI CCAn_nnnn'nTGG
Cuts at: 537 556 1817 537
Size: 19 1261 3717
Fragments arranged by size:
3717 1261 19
BstYI r'GATC_y
Cuts at: 1 312 455 2022 2489 2512 2847 3093 3399
Size: 311 143 1567 467 23 335 246 306
Cuts at: 3399 3411 3497 4549 1
Size: 12 86 1052 449
Fragments arranged by size:
1567 1052 467 449 335 311 306 246
143 86 23 12
BtgI C'CryG_G
Cuts at: 107 386 530 975 1051 107
Size: 279 144 445 76 4053
Fragments arranged by size:
4053 445 279 144 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2947 3128 3291 2947
Size: 181 163 4653
Fragments arranged by size:
4653 181 163
BtsI GCAGTG_nn'
Cuts at: 1014 2240 1014
Size: 1226 3771
DraI TTT'AAA
Cuts at: 1976 1996 1976
Size: 20 4977
DraIII CAC_nnn'GTG
Cuts at: 422 422
Size: 4997
DrdI GACnn_nn'nnGTC
Cuts at: 806 2703 4047 806
Size: 1897 1344 1756
Fragments arranged by size:
1897 1756 1344
EaeI y'GGCC_r
Cuts at: 155 903 1048 2582 2756 3147 3174 4519 155
Size: 748 145 1534 174 391 27 1345 633
Fragments arranged by size:
1534 1345 748 633 391 174 145 27
EagI C'GGCC_G
Cuts at: 2582 2582
Size: 4997
EarI CTCTTCn'nnn_
Cuts at: 830 3020 3230 830
Size: 2190 210 2597
Fragments arranged by size:
2597 2190 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3205 3947 4093 4807 3205
Size: 742 146 714 3395
Fragments arranged by size:
3395 742 714 146
EcoICRI GAG'CTC
Cuts at: 2009 2009
Size: 4997
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 648 704 784 921 1256 1798 2822 3254
Size: 448 56 80 137 335 542 1024 432
Cuts at: 3254 3607 4269 200
Size: 353 662 928
Fragments arranged by size:
1024 928 662 542 448 432 353 335
137 80 56
EcoO109I rG'GnC_Cy
Cuts at: 951 2136 951
Size: 1185 3812
EcoRI G'AATT_C
Cuts at: 478 2001 478
Size: 1523 3474
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1254 3016 1254
Size: 1762 3235
FspI TGC'GCA
Cuts at: 1589 2778 1589
Size: 1189 3808
HaeII r_GCGC'y
Cuts at: 644 2431 2679 3909 4680 644
Size: 1787 248 1230 771 961
Fragments arranged by size:
1787 1230 961 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 440 472 1301 1333 1478 1510 2839 2869 2871
Size: 32 829 32 145 32 1329 30 2
Cuts at: 2871 2901 440
Size: 30 2536
Fragments arranged by size:
2536 1329 829 145 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1447 2036 1447
Size: 589 4408
HindIII A'AGCT_T
Cuts at: 704 1923 704
Size: 1219 3778
KasI G'GCGC_C
Cuts at: 2427 2675 2427
Size: 248 4749
KpnI G_GTAC'C
Cuts at: 2017 2017
Size: 4997
MluI A'CGCG_T
Cuts at: 4430 4430
Size: 4997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3756 3940 3756
Size: 184 4813
MscI TGG'CCA
Cuts at: 905 2758 905
Size: 1853 3144
MslI CAynn'nnrTG
Cuts at: 151 2986 3113 151
Size: 2835 127 2035
Fragments arranged by size:
2835 2035 127
MspA1I CmG'CkG
Cuts at: 194 762 789 890 1063 1315 2108 2422 2782
Size: 568 27 101 173 252 793 314 360
Cuts at: 2782 3566 3811 194
Size: 784 245 1380
Fragments arranged by size:
1380 793 784 568 360 314 252 245
173 101 27
NaeI GCC'GGC
Cuts at: 524 3178 524
Size: 2654 2343
NarI GG'CG_CC
Cuts at: 2428 2676 2428
Size: 248 4749
NcoI C'CATG_G
Cuts at: 107 386 530 975 1051 107
Size: 279 144 445 76 4053
Fragments arranged by size:
4053 445 279 144 76
NdeI CA'TA_TG
Cuts at: 1683 1683
Size: 4997
NgoMIV G'CCGG_C
Cuts at: 522 3176 522
Size: 2654 2343
NsiI A_TGCA'T
Cuts at: 922 922
Size: 4997
NspI r_CATG'y
Cuts at: 1120 2050 2550 4153 1120
Size: 930 500 1603 1964
Fragments arranged by size:
1964 1603 930 500
PciI A'CATG_T
Cuts at: 4149 4149
Size: 4997
PflMI CCAn_nnn'nTGG
Cuts at: 682 682
Size: 4997
PmeI GTTT'AAAC
Cuts at: 1996 1996
Size: 4997
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 3443
Size: 32 4965
PshAI GACnn'nnGTC
Cuts at: 1541 1541
Size: 4997
PsiI TTA'TAA
Cuts at: 578 578
Size: 4997
PstI C_TGCA'G
Cuts at: 2044 2729 2044
Size: 685 4312
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4997
PvuII CAG'CTG
Cuts at: 762 789 890 1063 1315 2422 2782 762
Size: 27 101 173 252 1107 360 2977
Fragments arranged by size:
2977 1107 360 252 173 101 27
RsrII CG'GwC_CG
Cuts at: 3192 3192
Size: 4997
SacI G_AGCT'C
Cuts at: 2011 2011
Size: 4997
SalI G'TCGA_C
Cuts at: 1445 2034 1445
Size: 589 4408
SapI GCTCTTCn'nnn_
Cuts at: 830 3020 3230 830
Size: 2190 210 2597
Fragments arranged by size:
2597 2190 210
SbfI CC_TGCA'GG
Cuts at: 2044 2044
Size: 4997
ScaI AGT'ACT
Cuts at: 583 4514 583
Size: 3931 1066
SfcI C'TryA_G
Cuts at: 33 297 2040 2725 3693 3884 33
Size: 264 1743 685 968 191 1146
Fragments arranged by size:
1743 1146 968 685 264 191
SfoI GGC'GCC
Cuts at: 2429 2677 2429
Size: 248 4749
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4997
SmaI CCC'GGG
Cuts at: 2019 2019
Size: 4997
SmlI C'TyrA_G
Cuts at: 2152 3067 3781 4043 2152
Size: 915 714 262 3106
Fragments arranged by size:
3106 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4997
SphI G_CATG'C
Cuts at: 1120 2050 2550 1120
Size: 930 500 3567
Fragments arranged by size:
3567 930 500
SspI AAT'ATT
Cuts at: 1678 1678
Size: 4997
StyI C'CwwG_G
Cuts at: 107 386 530 975 1051 1655 2131 2485 107
Size: 279 144 445 76 604 476 354 2619
Fragments arranged by size:
2619 604 476 445 354 279 144 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2580 2713 3172 3381 2580
Size: 133 459 209 4196
Fragments arranged by size:
4196 459 209 133
TatI w'GTAC_w
Cuts at: 287 581 649 4512 287
Size: 294 68 3863 772
Fragments arranged by size:
3863 772 294 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1155 1187 4514 4546 1155
Size: 32 3327 32 1606
Fragments arranged by size:
3327 1606 32 32
Tth111I GACn'n_nGTC
Cuts at: 2794 2794
Size: 4997
XbaI T'CTAG_A
Cuts at: 2028 2028
Size: 4997
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 973 1574 2492 973
Size: 601 918 3478
Fragments arranged by size:
3478 918 601
XmaI C'CCGG_G
Cuts at: 2017 2017
Size: 4997
XmnI GAAnn'nnTTC
Cuts at: 1783 1783
Size: 4997
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI ApaI AscI
AvrII BbsI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BspEI BsrGI BstEII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI PacI PasI PfoI PmlI PpuMI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI