Restriction map for
pF1KB4576
(Circular) (Six-base) MAPSORT of: pF1KB4576.seq Check: 4082 from: 1 to: 3680
pF1KB4576 3680 bp
With 182 enzymes: SgfI *
February 15, 2010 17:49 ..
AccI GT'mk_AC
Cuts at: 718 718
Size: 3680
Acc65I G'GTAC_C
Cuts at: 696 696
Size: 3680
AclI AA'CG_TT
Cuts at: 3520 3520
Size: 3680
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 1505 1937 2684 2952 193
Size: 1312 432 747 268 921
Fragments arranged by size:
1312 921 747 432 268
AflIII A'CryG_T
Cuts at: 1032 2136 3113 1032
Size: 1104 977 1599
Fragments arranged by size:
1599 1104 977
AgeI A'CCGG_T
Cuts at: 2068 2068
Size: 3680
AlwNI CAG_nnn'CTG
Cuts at: 299 985 2552 299
Size: 686 1567 1427
Fragments arranged by size:
1567 1427 686
ApaLI G'TGCA_C
Cuts at: 2450 2450
Size: 3680
ApoI r'AATT_y
Cuts at: 684 3033 3289 684
Size: 2349 256 1075
Fragments arranged by size:
2349 1075 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3680
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3680
AvaI C'yCGr_G
Cuts at: 700 700
Size: 3680
BamHI G'GATC_C
Cuts at: 587 705 587
Size: 118 3562
BanI G'GyrC_C
Cuts at: 430 696 1110 1358 1393 430
Size: 266 414 248 35 2717
Fragments arranged by size:
2717 414 266 248 35
BanII G_rGCy'C
Cuts at: 293 393 694 1724 293
Size: 100 301 1030 2249
Fragments arranged by size:
2249 1030 301 100
BbeI G_GCGC'C
Cuts at: 1114 1362 1114
Size: 248 3432
BbsI GAAGACnn'nnnn_
Cuts at: 181 666 181
Size: 485 3195
BbvCI CC'TCA_GC
Cuts at: 439 439
Size: 3680
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 171 205 171
Size: 34 3646
BciVI GTATCCnnnnn_n'
Cuts at: 1578 2339 3680 1578
Size: 761 1341 1578
Fragments arranged by size:
1578 1341 761
BclI T'GATC_A
Cuts at: 452 1200 452
Size: 748 2932
BglI GCCn_nnn'nGGC
Cuts at: 112 288 343 425 112
Size: 176 55 82 3367
Fragments arranged by size:
3367 176 82 55
BglII A'GATC_T
Cuts at: 1195 3232 1195
Size: 2037 1643
BlpI GC'TnA_GC
Cuts at: 792 792
Size: 3680
Bme1580I G_kGCm'C
Cuts at: 435 1305 1398 2454 435
Size: 870 93 1056 1661
Fragments arranged by size:
1661 1056 870 93
BmrI ACTGGGnnnn_n'
Cuts at: 892 973 1308 3452 892
Size: 81 335 2144 1120
Fragments arranged by size:
2144 1120 335 81
BmtI G_CTAG'C
Cuts at: 562 562
Size: 3680
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 3680
Bpu10I CC'TnA_GC
Cuts at: 292 439 292
Size: 147 3533
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 1735 2227 2525 856
Size: 879 492 298 2011
Fragments arranged by size:
2011 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3384 3384
Size: 3680
BsaAI yAC'GTr
Cuts at: 1033 1033
Size: 3680
BsaBI GATnn'nnATC
Cuts at: 6 1194 3231 6
Size: 1188 2037 455
Fragments arranged by size:
2037 1188 455
BsaHI Gr'CG_yC
Cuts at: 1111 1359 2061 3557 1111
Size: 248 702 1496 1234
Fragments arranged by size:
1496 1234 702 248
BsaWI w'CCGG_w
Cuts at: 869 1390 2068 2342 2489 2900 869
Size: 521 678 274 147 411 1649
Fragments arranged by size:
1649 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 380 410 380
Size: 30 3650
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 427 427
Size: 3680
BseYI C'CCAG_C
Cuts at: 1105 1863 2440 2936 1105
Size: 758 577 496 1849
Fragments arranged by size:
1849 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 657 1222 1268 2476 104
Size: 553 565 46 1208 1308
Fragments arranged by size:
1308 1208 565 553 46
BsiHKAI G_wGCw'C
Cuts at: 323 694 1472 1662 2454 323
Size: 371 778 190 792 1549
Fragments arranged by size:
1549 792 778 371 190
BsmBI CGTCTCn'nnnn_
Cuts at: 315 559 315
Size: 244 3436
Bsp1286I G_dGCh'C
Cuts at: 293 323 393 435 694 1305 1398 1472 1662
Size: 30 70 42 259 611 93 74 190
Cuts at: 1662 1724 2454 293
Size: 62 730 1519
Fragments arranged by size:
1519 730 611 259 190 93 74 70
62 42 30
BspEI T'CCGG_A
Cuts at: 2900 2900
Size: 3680
BspHI T'CATG_A
Cuts at: 2856 2856
Size: 3680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 730 944 1246 1627 730
Size: 214 302 381 2783
Fragments arranged by size:
2783 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1972 2026 3510 3675 50
Size: 1922 54 1484 165 55
Fragments arranged by size:
1922 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 261 332 1592 261
Size: 71 1260 2349
Fragments arranged by size:
2349 1260 71
BsrFI r'CCGG_y
Cuts at: 1678 1859 2068 1678
Size: 181 209 3290
Fragments arranged by size:
3290 209 181
BssSI C'ACGA_G
Cuts at: 160 1951 2309 160
Size: 1791 358 1531
Fragments arranged by size:
1791 1531 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1057 1057
Size: 3680
BstBI TT'CG_AA
Cuts at: 2041 2041
Size: 3680
BstEII G'GTnAC_C
Cuts at: 396 396
Size: 3680
BstYI r'GATC_y
Cuts at: 1 185 587 705 1172 1195 1530 1776 2788
Size: 184 402 118 467 23 335 246 1012
Cuts at: 2788 2874 2886 3232 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 402 346 335 246 184
118 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3680
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 211 1630 1811 1974 211
Size: 1419 181 163 1917
Fragments arranged by size:
1917 1419 181 163
BtsI GCAGTG_nn'
Cuts at: 548 923 548
Size: 375 3305
DraI TTT'AAA
Cuts at: 679 679
Size: 3680
DrdI GACnn_nn'nnGTC
Cuts at: 1386 2244 1386
Size: 858 2822
EaeI y'GGCC_r
Cuts at: 113 654 1265 1439 1830 1857 3202 113
Size: 541 611 174 391 27 1345 591
Fragments arranged by size:
1345 611 591 541 391 174 27
EagI C'GGCC_G
Cuts at: 654 1265 654
Size: 611 3069
EarI CTCTTCn'nnn_
Cuts at: 328 1703 1913 328
Size: 1375 210 2095
Fragments arranged by size:
2095 1375 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 406 1888 2198 2344 3490 406
Size: 1482 310 146 1146 596
Fragments arranged by size:
1482 1146 596 310 146
EcoICRI GAG'CTC
Cuts at: 692 692
Size: 3680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 1505 1937 2684 2952 193
Size: 1312 432 747 268 921
Fragments arranged by size:
1312 921 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 819 819
Size: 3680
EcoRI G'AATT_C
Cuts at: 684 684
Size: 3680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1699 1699
Size: 3680
FspI TGC'GCA
Cuts at: 1461 1461
Size: 3680
HaeII r_GCGC'y
Cuts at: 349 1114 1362 2384 3363 349
Size: 765 248 1022 979 666
Fragments arranged by size:
1022 979 765 666 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1522 1552 1554 1584 1522
Size: 30 2 30 3618
Fragments arranged by size:
3618 30 30 2
HincII GTy'rAC
Cuts at: 719 719
Size: 3680
HindIII A'AGCT_T
Cuts at: 515 515
Size: 3680
KasI G'GCGC_C
Cuts at: 1110 1358 1110
Size: 248 3432
KpnI G_GTAC'C
Cuts at: 700 700
Size: 3680
MluI A'CGCG_T
Cuts at: 3113 3113
Size: 3680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2351 2535 2351
Size: 184 3496
MscI TGG'CCA
Cuts at: 1441 1441
Size: 3680
MslI CAynn'nnrTG
Cuts at: 1669 1796 1669
Size: 127 3553
MspA1I CmG'CkG
Cuts at: 791 1105 1465 2478 2723 791
Size: 314 360 1013 245 1748
Fragments arranged by size:
1748 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1861 1861
Size: 3680
NarI GG'CG_CC
Cuts at: 1111 1359 1111
Size: 248 3432
NcoI C'CATG_G
Cuts at: 107 107
Size: 3680
NgoMIV G'CCGG_C
Cuts at: 1859 1859
Size: 3680
NheI G'CTAG_C
Cuts at: 558 558
Size: 3680
NspI r_CATG'y
Cuts at: 733 1233 2140 733
Size: 500 907 2273
Fragments arranged by size:
2273 907 500
PasI CC'CwG_GG
Cuts at: 582 582
Size: 3680
PciI A'CATG_T
Cuts at: 2136 2136
Size: 3680
PflMI CCAn_nnn'nTGG
Cuts at: 540 540
Size: 3680
PmeI GTTT'AAAC
Cuts at: 679 679
Size: 3680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2819 2851 2819
Size: 32 3648
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 454 486 454
Size: 32 3648
PstI C_TGCA'G
Cuts at: 727 1412 727
Size: 685 2995
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3680
PvuII CAG'CTG
Cuts at: 1105 1465 1105
Size: 360 3320
RsrII CG'GwC_CG
Cuts at: 1875 1875
Size: 3680
SacI G_AGCT'C
Cuts at: 694 694
Size: 3680
SalI G'TCGA_C
Cuts at: 717 717
Size: 3680
SapI GCTCTTCn'nnn_
Cuts at: 328 1703 1913 328
Size: 1375 210 2095
Fragments arranged by size:
2095 1375 210
SbfI CC_TGCA'GG
Cuts at: 727 727
Size: 3680
ScaI AGT'ACT
Cuts at: 3197 3197
Size: 3680
SfcI C'TryA_G
Cuts at: 33 723 1408 2401 2592 33
Size: 690 685 993 191 1121
Fragments arranged by size:
1121 993 690 685 191
SfoI GGC'GCC
Cuts at: 1112 1360 1112
Size: 248 3432
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3680
SmaI CCC'GGG
Cuts at: 702 702
Size: 3680
SmlI C'TyrA_G
Cuts at: 835 1750 2242 2504 835
Size: 915 492 262 2011
Fragments arranged by size:
2011 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3680
SphI G_CATG'C
Cuts at: 733 1233 733
Size: 500 3180
StyI C'CwwG_G
Cuts at: 107 814 1168 107
Size: 707 354 2619
Fragments arranged by size:
2619 707 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1263 1396 1855 2064 1263
Size: 133 459 209 2879
Fragments arranged by size:
2879 459 209 133
TatI w'GTAC_w
Cuts at: 3195 3195
Size: 3680
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 380 412 3197 3229 380
Size: 32 2785 32 831
Fragments arranged by size:
2785 831 32 32
Tth111I GACn'n_nGTC
Cuts at: 1477 1477
Size: 3680
XbaI T'CTAG_A
Cuts at: 711 711
Size: 3680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1175 1175
Size: 3680
XmaI C'CCGG_G
Cuts at: 700 700
Size: 3680
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PmeI PpiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BfrBI BmgBI BpmI BsgI
BsiWI BsmI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NotI NruI NsiI PacI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI