Restriction map for
pF1KB4543
(Circular) (Six-base) MAPSORT of: pF1KB4543.seq Check: 3837 from: 1 to: 6470
pF1KB4543 6470 bp
With 182 enzymes: SgfI *
April 14, 2015 09:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 441 2508 441
Size: 2067 4403
AatII G_ACGT'C
Cuts at: 3366 3366
Size: 6470
AccI GT'mk_AC
Cuts at: 1778 1985 2284 3508 1778
Size: 207 299 1224 4740
Fragments arranged by size:
4740 1224 299 207
Acc65I G'GTAC_C
Cuts at: 3486 3486
Size: 6470
AclI AA'CG_TT
Cuts at: 929 1772 6310 929
Size: 843 4538 1089
Fragments arranged by size:
4538 1089 843
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 477 979 1286 1897 1903 3337 4295 4727
Size: 164 502 307 611 6 1434 958 432
Cuts at: 4727 5474 5742 313
Size: 747 268 1041
Fragments arranged by size:
1434 1041 958 747 611 502 432 307
268 164 6
AflIII A'CryG_T
Cuts at: 782 1358 1887 3822 4926 5903 782
Size: 576 529 1935 1104 977 1349
Fragments arranged by size:
1935 1349 1104 977 576 529
AgeI A'CCGG_T
Cuts at: 4858 4858
Size: 6470
AhdI GACnn_n'nnGTC
Cuts at: 1362 1362
Size: 6470
AleI CACnn'nnGTG
Cuts at: 619 1217 1258 1276 619
Size: 598 41 18 5813
Fragments arranged by size:
5813 598 41 18
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1077 2278 1077
Size: 1201 5269
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 235 267 235
Size: 32 6438
AlwNI CAG_nnn'CTG
Cuts at: 1077 3435 3451 3775 5342 1077
Size: 2358 16 324 1567 2205
Fragments arranged by size:
2358 2205 1567 324 16
ApaI G_GGCC'C
Cuts at: 365 365
Size: 6470
ApaLI G'TGCA_C
Cuts at: 397 1062 5240 397
Size: 665 4178 1627
Fragments arranged by size:
4178 1627 665
ApoI r'AATT_y
Cuts at: 3474 5823 6079 3474
Size: 2349 256 3865
Fragments arranged by size:
3865 2349 256
AscI GG'CGCG_CC
Cuts at: 353 353
Size: 6470
AseI AT'TA_AT
Cuts at: 20 20
Size: 6470
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6470
AvaI C'yCGr_G
Cuts at: 160 545 1156 1788 2081 2913 3490 160
Size: 385 611 632 293 832 577 3140
Fragments arranged by size:
3140 832 632 611 577 385 293
BamHI G'GATC_C
Cuts at: 3495 3495
Size: 6470
BanI G'GyrC_C
Cuts at: 117 166 453 751 2102 2317 2440 3197 3486
Size: 49 287 298 1351 215 123 757 289
Cuts at: 3486 3900 4148 4183 117
Size: 414 248 35 2404
Fragments arranged by size:
2404 1351 757 414 298 289 287 248
215 123 49 35
BanII G_rGCy'C
Cuts at: 161 206 365 388 674 1100 2209 2384 2414
Size: 45 159 23 286 426 1109 175 30
Cuts at: 2414 2828 3410 3484 4514 161
Size: 414 582 74 1030 2117
Fragments arranged by size:
2117 1109 1030 582 426 414 286 175
159 74 45 30 23
BbeI G_GCGC'C
Cuts at: 121 170 3904 4152 121
Size: 49 3734 248 2439
Fragments arranged by size:
3734 2439 248 49
BbvCI CC'TCA_GC
Cuts at: 2533 2533
Size: 6470
BciVI GTATCCnnnnn_n'
Cuts at: 2413 4368 5129 6470 2413
Size: 1955 761 1341 2413
Fragments arranged by size:
2413 1955 1341 761
BclI T'GATC_A
Cuts at: 3990 3990
Size: 6470
BglI GCCn_nnn'nGGC
Cuts at: 175 2315 2560 3356 175
Size: 2140 245 796 3289
Fragments arranged by size:
3289 2140 796 245
BglII A'GATC_T
Cuts at: 3985 6022 3985
Size: 2037 4433
BlpI GC'TnA_GC
Cuts at: 2413 3582 2413
Size: 1169 5301
Bme1580I G_kGCm'C
Cuts at: 365 401 458 1066 1795 2320 3202 3379 4095
Size: 36 57 608 729 525 882 177 716
Cuts at: 4095 4188 5244 365
Size: 93 1056 1591
Fragments arranged by size:
1591 1056 882 729 716 608 525 177
93 57 36
BmgBI CAC'GTC
Cuts at: 733 1359 2390 2640 733
Size: 626 1031 250 4563
Fragments arranged by size:
4563 1031 626 250
BmrI ACTGGGnnnn_n'
Cuts at: 1053 2441 3084 3682 3763 4098 6242 1053
Size: 1388 643 598 81 335 2144 1281
Fragments arranged by size:
2144 1388 1281 643 598 335 81
BmtI G_CTAG'C
Cuts at: 2332 2905 2332
Size: 573 5897
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4837 4837
Size: 6470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 295 759 895 1867 2223 2428 3086 262
Size: 33 464 136 972 356 205 658 3646
Fragments arranged by size:
3646 972 658 464 356 205 136 33
Bpu10I CC'TnA_GC
Cuts at: 2533 2950 2533
Size: 417 6053
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3646 4525 5017 5315 3646
Size: 879 492 298 4801
Fragments arranged by size:
4801 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6174 6174
Size: 6470
BsaAI yAC'GTr
Cuts at: 3823 3823
Size: 6470
BsaBI GATnn'nnATC
Cuts at: 6 991 3984 6021 6
Size: 985 2993 2037 455
Fragments arranged by size:
2993 2037 985 455
BsaHI Gr'CG_yC
Cuts at: 118 167 2711 3363 3389 3901 4149 4851 6347
Size: 49 2544 652 26 512 248 702 1496
Cuts at: 6347 118
Size: 241
Fragments arranged by size:
2544 1496 702 652 512 248 241 49
26
BsaWI w'CCGG_w
Cuts at: 440 3659 4180 4858 5132 5279 5690 440
Size: 3219 521 678 274 147 411 1220
Fragments arranged by size:
3219 1220 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 235 265 1891 1921 235
Size: 30 1626 30 4784
Fragments arranged by size:
4784 1626 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 895 1078 2581 2584 2931 235
Size: 660 183 1503 3 347 3774
Fragments arranged by size:
3774 1503 660 347 183 3
BseYI C'CCAG_C
Cuts at: 348 458 531 565 1190 2645 3403 3451 3895
Size: 110 73 34 625 1455 758 48 444
Cuts at: 3895 4653 5230 5726 348
Size: 758 577 496 1092
Fragments arranged by size:
1455 1092 758 758 625 577 496 444
110 73 48 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1288 1654 2056 1288
Size: 366 402 5702
Fragments arranged by size:
5702 402 366
BsiEI CG_ry'CG
Cuts at: 104 182 581 4012 4058 5266 104
Size: 78 399 3431 46 1208 1308
Fragments arranged by size:
3431 1308 1208 399 78 46
BsiHKAI G_wGCw'C
Cuts at: 401 1066 1100 2209 2414 2828 2881 2956 3484
Size: 665 34 1109 205 414 53 75 528
Cuts at: 3484 4262 4452 5244 401
Size: 778 190 792 1627
Fragments arranged by size:
1627 1109 792 778 665 528 414 205
190 75 53 34
BsmBI CGTCTCn'nnnn_
Cuts at: 739 2451 3396 739
Size: 1712 945 3813
Fragments arranged by size:
3813 1712 945
Bsp1286I G_dGCh'C
Cuts at: 161 206 365 388 401 458 674 1066 1100
Size: 45 159 23 13 57 216 392 34
Cuts at: 1100 1795 2209 2320 2384 2414 2828 2881 2956
Size: 695 414 111 64 30 414 53 75
Cuts at: 2956 3202 3379 3410 3484 4095 4188 4262 4452
Size: 246 177 31 74 611 93 74 190
Cuts at: 4452 4514 5244 161
Size: 62 730 1387
Fragments arranged by size:
1387 730 695 611 414 414 392 246
216 190 177 159 111 93 75 74
74 64 62 57 53 45 34 31
30 23 13
BspEI T'CCGG_A
Cuts at: 5690 5690
Size: 6470
BspHI T'CATG_A
Cuts at: 908 1688 5646 908
Size: 780 3958 1732
Fragments arranged by size:
3958 1732 780
BspMI ACCTGCnnnn'nnnn_
Cuts at: 441 459 1634 1702 2078 2508 3443 3520 3734
Size: 18 1175 68 376 430 935 77 214
Cuts at: 3734 4036 4417 441
Size: 302 381 2494
Fragments arranged by size:
2494 1175 935 430 381 376 302 214
77 68 18
BsrBI CCG'CTC
Cuts at: 50 174 900 1754 4762 4816 6300 6465 50
Size: 124 726 854 3008 54 1484 165 55
Fragments arranged by size:
3008 1484 854 726 165 124 55 54
BsrDI GCAATG_nn'
Cuts at: 4382 4382
Size: 6470
BsrFI r'CCGG_y
Cuts at: 194 1590 2631 3182 4468 4649 4858 194
Size: 1396 1041 551 1286 181 209 1806
Fragments arranged by size:
1806 1396 1286 1041 551 209 181
BssHII G'CGCG_C
Cuts at: 326 353 326
Size: 27 6443
BssSI C'ACGA_G
Cuts at: 424 673 4741 5099 424
Size: 249 4068 358 1795
Fragments arranged by size:
4068 1795 358 249
BstAPI GCAn_nnn'nTGC
Cuts at: 1641 3847 1641
Size: 2206 4264
BstBI TT'CG_AA
Cuts at: 4831 4831
Size: 6470
BstEII G'GTnAC_C
Cuts at: 429 612 429
Size: 183 6287
BstXI CCAn_nnnn'nTGG
Cuts at: 737 1278 1392 2520 3132 737
Size: 541 114 1128 612 4075
Fragments arranged by size:
4075 1128 612 541 114
BstYI r'GATC_y
Cuts at: 1 1281 1670 3495 3962 3985 4320 4566 5578
Size: 1280 389 1825 467 23 335 246 1012
Cuts at: 5578 5664 5676 6022 1
Size: 86 12 346 449
Fragments arranged by size:
1825 1280 1012 467 449 389 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 255 935 1124 107
Size: 148 680 189 5453
Fragments arranged by size:
5453 680 189 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1531 2793 4420 4601 4764 1531
Size: 1262 1627 181 163 3237
Fragments arranged by size:
3237 1627 1262 181 163
BtsI GCAGTG_nn'
Cuts at: 399 1212 1644 3713 399
Size: 813 432 2069 3156
Fragments arranged by size:
3156 2069 813 432
DraI TTT'AAA
Cuts at: 3469 3469
Size: 6470
DraIII CAC_nnn'GTG
Cuts at: 396 2008 3213 396
Size: 1612 1205 3653
Fragments arranged by size:
3653 1612 1205
DrdI GACnn_nn'nnGTC
Cuts at: 4176 5034 4176
Size: 858 5612
EaeI y'GGCC_r
Cuts at: 179 284 302 338 377 474 656 1382 1592
Size: 105 18 36 39 97 182 726 210
Cuts at: 1592 2474 2888 3122 4055 4229 4620 4647 5992
Size: 882 414 234 933 174 391 27 1345
Cuts at: 5992 179
Size: 657
Fragments arranged by size:
1345 933 882 726 657 414 391 234
210 182 174 105 97 39 36 27
18
EagI C'GGCC_G
Cuts at: 179 4055 179
Size: 3876 2594
EarI CTCTTCn'nnn_
Cuts at: 415 496 772 1105 2491 2623 4493 4703 415
Size: 81 276 333 1386 132 1870 210 2182
Fragments arranged by size:
2182 1870 1386 333 276 210 132 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 143 155 543 2977 4678 4988 5134 6280 143
Size: 12 388 2434 1701 310 146 1146 333
Fragments arranged by size:
2434 1701 1146 388 333 310 146 12
EcoICRI GAG'CTC
Cuts at: 1098 2207 2412 2826 3482 1098
Size: 1109 205 414 656 4086
Fragments arranged by size:
4086 1109 656 414 205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 295 313 477 759 895 979 1286 1867
Size: 33 18 164 282 136 84 307 581
Cuts at: 1867 1897 1903 2223 2428 3086 3337 4295 4727
Size: 30 6 320 205 658 251 958 432
Cuts at: 4727 5474 5742 262
Size: 747 268 990
Fragments arranged by size:
990 958 747 658 581 432 320 307
282 268 251 205 164 136 84 33
30 18 6
EcoNI CCTnn'n_nnAGG
Cuts at: 379 379
Size: 6470
EcoO109I rG'GnC_Cy
Cuts at: 361 1317 3086 3305 3609 361
Size: 956 1769 219 304 3222
Fragments arranged by size:
3222 1769 956 304 219
EcoRI G'AATT_C
Cuts at: 3474 3474
Size: 6470
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 969 4489 969
Size: 3520 2950
FspI TGC'GCA
Cuts at: 646 1342 4251 646
Size: 696 2909 2865
Fragments arranged by size:
2909 2865 696
HaeII r_GCGC'y
Cuts at: 121 170 250 301 337 421 487 729 1943
Size: 49 80 51 36 84 66 242 1214
Cuts at: 1943 2908 3080 3904 4152 5174 6153 121
Size: 965 172 824 248 1022 979 438
Fragments arranged by size:
1214 1022 979 965 824 438 248 242
172 84 80 66 51 49 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1348 1380 3026 3058 3063 3095 3212 3244 4312
Size: 32 1646 32 5 32 117 32 1068
Cuts at: 4312 4342 4344 4374 1348
Size: 30 2 30 3444
Fragments arranged by size:
3444 1646 1068 117 32 32 32 32
30 30 5 2
HincII GTy'rAC
Cuts at: 1803 3117 3257 3509 1803
Size: 1314 140 252 4764
Fragments arranged by size:
4764 1314 252 140
KasI G'GCGC_C
Cuts at: 117 166 3900 4148 117
Size: 49 3734 248 2439
Fragments arranged by size:
3734 2439 248 49
KpnI G_GTAC'C
Cuts at: 3490 3490
Size: 6470
MluI A'CGCG_T
Cuts at: 5903 5903
Size: 6470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2687 3109 5141 5325 2687
Size: 422 2032 184 3832
Fragments arranged by size:
3832 2032 422 184
MscI TGG'CCA
Cuts at: 379 658 1384 2476 3124 4231 379
Size: 279 726 1092 648 1107 2618
Fragments arranged by size:
2618 1107 1092 726 648 279
MslI CAynn'nnrTG
Cuts at: 619 940 1007 1059 1217 1258 1276 1294 2776
Size: 321 67 52 158 41 18 18 1482
Cuts at: 2776 2884 2977 4459 4586 619
Size: 108 93 1482 127 2503
Fragments arranged by size:
2503 1482 1482 321 158 127 108 93
67 52 41 18 18
MspA1I CmG'CkG
Cuts at: 257 462 480 565 1402 2417 2893 3451 3581
Size: 205 18 85 837 1015 476 558 130
Cuts at: 3581 3895 4255 5268 5513 257
Size: 314 360 1013 245 1214
Fragments arranged by size:
1214 1015 1013 837 558 476 360 314
245 205 130 85 18
NaeI GCC'GGC
Cuts at: 196 2633 4651 196
Size: 2437 2018 2015
Fragments arranged by size:
2437 2018 2015
NarI GG'CG_CC
Cuts at: 118 167 3901 4149 118
Size: 49 3734 248 2439
Fragments arranged by size:
3734 2439 248 49
NcoI C'CATG_G
Cuts at: 107 935 107
Size: 828 5642
NgoMIV G'CCGG_C
Cuts at: 194 2631 4649 194
Size: 2437 2018 2015
Fragments arranged by size:
2437 2018 2015
NheI G'CTAG_C
Cuts at: 2328 2901 2328
Size: 573 5897
NspI r_CATG'y
Cuts at: 786 1293 1891 2512 3523 4023 4930 786
Size: 507 598 621 1011 500 907 2326
Fragments arranged by size:
2326 1011 907 621 598 507 500
PasI CC'CwG_GG
Cuts at: 3309 3309
Size: 6470
PciI A'CATG_T
Cuts at: 782 1887 4926 782
Size: 1105 3039 2326
Fragments arranged by size:
3039 2326 1105
PflMI CCAn_nnn'nTGG
Cuts at: 1077 1077
Size: 6470
PmeI GTTT'AAAC
Cuts at: 3469 3469
Size: 6470
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 235 267 5609 5641 235
Size: 32 5342 32 1064
Fragments arranged by size:
5342 1064 32 32
PpuMI rG'GwC_Cy
Cuts at: 1317 3305 1317
Size: 1988 4482
PspOMI G'GGCC_C
Cuts at: 361 361
Size: 6470
PstI C_TGCA'G
Cuts at: 452 467 842 3447 3517 4202 452
Size: 15 375 2605 70 685 2720
Fragments arranged by size:
2720 2605 685 375 70 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6470
PvuII CAG'CTG
Cuts at: 462 480 3451 3895 4255 462
Size: 18 2971 444 360 2677
Fragments arranged by size:
2971 2677 444 360 18
RsrII CG'GwC_CG
Cuts at: 4665 4665
Size: 6470
SacI G_AGCT'C
Cuts at: 1100 2209 2414 2828 3484 1100
Size: 1109 205 414 656 4086
Fragments arranged by size:
4086 1109 656 414 205
SacII CC_GC'GG
Cuts at: 258 258
Size: 6470
SalI G'TCGA_C
Cuts at: 3507 3507
Size: 6470
SapI GCTCTTCn'nnn_
Cuts at: 496 772 1105 2491 4493 4703 496
Size: 276 333 1386 2002 210 2263
Fragments arranged by size:
2263 2002 1386 333 276 210
SbfI CC_TGCA'GG
Cuts at: 452 842 3517 452
Size: 390 2675 3405
Fragments arranged by size:
3405 2675 390
ScaI AGT'ACT
Cuts at: 5987 5987
Size: 6470
SfcI C'TryA_G
Cuts at: 33 448 463 838 3443 3513 4198 5191 5382
Size: 415 15 375 2605 70 685 993 191
Cuts at: 5382 33
Size: 1121
Fragments arranged by size:
2605 1121 993 685 415 375 191 70
15
SfoI GGC'GCC
Cuts at: 119 168 3902 4150 119
Size: 49 3734 248 2439
Fragments arranged by size:
3734 2439 248 49
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6470
SgrAI Cr'CCGG_yG
Cuts at: 194 194
Size: 6470
SmaI CCC'GGG
Cuts at: 162 547 1790 2083 3492 162
Size: 385 1243 293 1409 3140
Fragments arranged by size:
3140 1409 1243 385 293
SmlI C'TyrA_G
Cuts at: 3625 4540 5032 5294 3625
Size: 915 492 262 4801
Fragments arranged by size:
4801 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6470
SphI G_CATG'C
Cuts at: 2512 3523 4023 2512
Size: 1011 500 4959
Fragments arranged by size:
4959 1011 500
SspI AAT'ATT
Cuts at: 1997 1997
Size: 6470
StuI AGG'CCT
Cuts at: 1568 1568
Size: 6470
StyI C'CwwG_G
Cuts at: 107 380 710 935 1379 2169 2194 2921 3604
Size: 273 330 225 444 790 25 727 683
Cuts at: 3604 3958 107
Size: 354 2619
Fragments arranged by size:
2619 790 727 683 444 354 330 273
225 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 878 1204 4053 4186 4645 4854 878
Size: 326 2849 133 459 209 2494
Fragments arranged by size:
2849 2494 459 326 209 133
TatI w'GTAC_w
Cuts at: 1880 5985 1880
Size: 4105 2365
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 735 767 2421 2453 5987 6019 735
Size: 32 1654 32 3534 32 1186
Fragments arranged by size:
3534 1654 1186 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3025 4267 3025
Size: 1242 5228
XbaI T'CTAG_A
Cuts at: 3267 3501 3267
Size: 234 6236
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1257 2223 3965 1257
Size: 966 1742 3762
Fragments arranged by size:
3762 1742 966
XmaI C'CCGG_G
Cuts at: 160 545 1788 2081 3490 160
Size: 385 1243 293 1409 3140
Fragments arranged by size:
3140 1409 1243 385 293
XmnI GAAnn'nnTTC
Cuts at: 832 1498 1657 832
Size: 666 159 5645
Fragments arranged by size:
5645 666 159
ZraI GAC'GTC
Cuts at: 3364 3364
Size: 6470
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AvrII BaeI BbsI BcgI BfrBI BsiWI
BsmI BsrGI BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI
PacI PfoI PmlI PshAI PsiI PspXI PsrI SanDI
SexAI SfiI SnaBI SrfI SwaI XhoI