Restriction map for
pF1KB4540
(Circular) (Six-base) MAPSORT of: pF1KB4540.seq Check: 4046 from: 1 to: 4157
pF1KB4540 4157 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 260 1195 260
Size: 935 3222
Acc65I G'GTAC_C
Cuts at: 1173 1173
Size: 4157
AclI AA'CG_TT
Cuts at: 3997 3997
Size: 4157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 1982 2414 3161 3429 490
Size: 1492 432 747 268 1218
Fragments arranged by size:
1492 1218 747 432 268
AflII C'TTAA_G
Cuts at: 118 118
Size: 4157
AflIII A'CryG_T
Cuts at: 991 1509 2613 3590 991
Size: 518 1104 977 1558
Fragments arranged by size:
1558 1104 977 518
AgeI A'CCGG_T
Cuts at: 2545 2545
Size: 4157
AlwNI CAG_nnn'CTG
Cuts at: 395 1462 3029 395
Size: 1067 1567 1523
Fragments arranged by size:
1567 1523 1067
ApaLI G'TGCA_C
Cuts at: 2927 2927
Size: 4157
ApoI r'AATT_y
Cuts at: 1161 3510 3766 1161
Size: 2349 256 1552
Fragments arranged by size:
2349 1552 256
AseI AT'TA_AT
Cuts at: 20 799 20
Size: 779 3378
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
AvaI C'yCGr_G
Cuts at: 1177 1177
Size: 4157
BamHI G'GATC_C
Cuts at: 1182 1182
Size: 4157
BanI G'GyrC_C
Cuts at: 1173 1587 1835 1870 1173
Size: 414 248 35 3460
Fragments arranged by size:
3460 414 248 35
BanII G_rGCy'C
Cuts at: 167 1171 2201 167
Size: 1004 1030 2123
Fragments arranged by size:
2123 1030 1004
BbeI G_GCGC'C
Cuts at: 1591 1839 1591
Size: 248 3909
BbsI GAAGACnn'nnnn_
Cuts at: 1154 1154
Size: 4157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4123
BciVI GTATCCnnnnn_n'
Cuts at: 2055 2816 4157 2055
Size: 761 1341 2055
Fragments arranged by size:
2055 1341 761
BclI T'GATC_A
Cuts at: 342 1677 342
Size: 1335 2822
BfrBI ATG'CAT
Cuts at: 425 425
Size: 4157
BglII A'GATC_T
Cuts at: 1672 3709 1672
Size: 2037 2120
BlpI GC'TnA_GC
Cuts at: 350 1269 350
Size: 919 3238
Bme1580I G_kGCm'C
Cuts at: 541 1782 1875 2931 541
Size: 1241 93 1056 1767
Fragments arranged by size:
1767 1241 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1369 1450 1785 3929 1369
Size: 81 335 2144 1597
Fragments arranged by size:
2144 1597 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 945 2524 945
Size: 1579 2578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 702 601
Size: 101 4056
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 191 1333 2212 2704 3002 151
Size: 40 1142 879 492 298 1306
Fragments arranged by size:
1306 1142 879 492 298 40
BsaI GGTCTCn'nnnn_
Cuts at: 121 774 3861 121
Size: 653 3087 417
Fragments arranged by size:
3087 653 417
BsaAI yAC'GTr
Cuts at: 1510 1510
Size: 4157
BsaBI GATnn'nnATC
Cuts at: 6 1671 3708 6
Size: 1665 2037 455
Fragments arranged by size:
2037 1665 455
BsaHI Gr'CG_yC
Cuts at: 1588 1836 2538 4034 1588
Size: 248 702 1496 1711
Fragments arranged by size:
1711 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1346 1867 2545 2819 2966 3377 1346
Size: 521 678 274 147 411 2126
Fragments arranged by size:
2126 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 254 604 254
Size: 350 3807
BseYI C'CCAG_C
Cuts at: 411 1582 2340 2917 3413 411
Size: 1171 758 577 496 1155
Fragments arranged by size:
1171 1155 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 313 408 462 684 768 211
Size: 102 95 54 222 84 3600
Fragments arranged by size:
3600 222 102 95 84 54
BsiEI CG_ry'CG
Cuts at: 104 664 1699 1745 2953 104
Size: 560 1035 46 1208 1308
Fragments arranged by size:
1308 1208 1035 560 46
BsiHKAI G_wGCw'C
Cuts at: 167 1171 1949 2139 2931 167
Size: 1004 778 190 792 1393
Fragments arranged by size:
1393 1004 792 778 190
BsmI GAATG_Cn'
Cuts at: 427 427
Size: 4157
Bsp1286I G_dGCh'C
Cuts at: 167 541 1171 1782 1875 1949 2139 2201 2931
Size: 374 630 611 93 74 190 62 730
Cuts at: 2931 167
Size: 1393
Fragments arranged by size:
1393 730 630 611 374 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3377 3377
Size: 4157
BspHI T'CATG_A
Cuts at: 3333 3333
Size: 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 740 830 1207 1421 1723 2104 740
Size: 90 377 214 302 381 2793
Fragments arranged by size:
2793 381 377 302 214 90
BsrBI CCG'CTC
Cuts at: 50 2449 2503 3987 4152 50
Size: 2399 54 1484 165 55
Fragments arranged by size:
2399 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2069 2069
Size: 4157
BsrFI r'CCGG_y
Cuts at: 1094 2155 2336 2545 1094
Size: 1061 181 209 2706
Fragments arranged by size:
2706 1061 209 181
BssSI C'ACGA_G
Cuts at: 2428 2786 2428
Size: 358 3799
BstAPI GCAn_nnn'nTGC
Cuts at: 1534 1534
Size: 4157
BstBI TT'CG_AA
Cuts at: 2518 2518
Size: 4157
BstYI r'GATC_y
Cuts at: 1 250 1182 1649 1672 2007 2253 3265 3351
Size: 249 932 467 23 335 246 1012 86
Cuts at: 3351 3363 3709 1
Size: 12 346 449
Fragments arranged by size:
1012 932 467 449 346 335 249 246
86 23 12
BtgI C'CryG_G
Cuts at: 704 762 704
Size: 58 4099
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2107 2288 2451 2107
Size: 181 163 3813
Fragments arranged by size:
3813 181 163
BtsI GCAGTG_nn'
Cuts at: 296 1400 296
Size: 1104 3053
DraI TTT'AAA
Cuts at: 1156 1156
Size: 4157
DrdI GACnn_nn'nnGTC
Cuts at: 1863 2721 1863
Size: 858 3299
EaeI y'GGCC_r
Cuts at: 501 548 661 1742 1916 2307 2334 3679 501
Size: 47 113 1081 174 391 27 1345 979
Fragments arranged by size:
1345 1081 979 391 174 113 47 27
EagI C'GGCC_G
Cuts at: 661 1742 661
Size: 1081 3076
EarI CTCTTCn'nnn_
Cuts at: 119 890 2180 2390 119
Size: 771 1290 210 1886
Fragments arranged by size:
1886 1290 771 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2365 2675 2821 3967 2365
Size: 310 146 1146 2555
Fragments arranged by size:
2555 1146 310 146
EcoICRI GAG'CTC
Cuts at: 165 1169 165
Size: 1004 3153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 601 702 1982 2414 3161 3429 490
Size: 111 101 1280 432 747 268 1218
Fragments arranged by size:
1280 1218 747 432 268 111 101
EcoO109I rG'GnC_Cy
Cuts at: 1296 1296
Size: 4157
EcoRI G'AATT_C
Cuts at: 1161 1161
Size: 4157
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 495 2176 495
Size: 1681 2476
FspI TGC'GCA
Cuts at: 1938 1938
Size: 4157
HaeII r_GCGC'y
Cuts at: 1591 1839 2861 3840 1591
Size: 248 1022 979 1908
Fragments arranged by size:
1908 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 162 194 289 321 1999 2029 2031 2061 162
Size: 32 95 32 1678 30 2 30 2258
Fragments arranged by size:
2258 1678 95 32 32 30 30 2
HincII GTy'rAC
Cuts at: 755 1119 1196 755
Size: 364 77 3716
Fragments arranged by size:
3716 364 77
HindIII A'AGCT_T
Cuts at: 209 209
Size: 4157
KasI G'GCGC_C
Cuts at: 1587 1835 1587
Size: 248 3909
KpnI G_GTAC'C
Cuts at: 1177 1177
Size: 4157
MluI A'CGCG_T
Cuts at: 3590 3590
Size: 4157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1000 2828 3012 1000
Size: 1828 184 2145
Fragments arranged by size:
2145 1828 184
MscI TGG'CCA
Cuts at: 503 550 1918 503
Size: 47 1368 2742
Fragments arranged by size:
2742 1368 47
MslI CAynn'nnrTG
Cuts at: 1067 2146 2273 1067
Size: 1079 127 2951
Fragments arranged by size:
2951 1079 127
MspA1I CmG'CkG
Cuts at: 675 722 1268 1582 1942 2955 3200 675
Size: 47 546 314 360 1013 245 1632
Fragments arranged by size:
1632 1013 546 360 314 245 47
NaeI GCC'GGC
Cuts at: 2338 2338
Size: 4157
NarI GG'CG_CC
Cuts at: 1588 1836 1588
Size: 248 3909
NcoI C'CATG_G
Cuts at: 704 762 704
Size: 58 4099
NgoMIV G'CCGG_C
Cuts at: 2336 2336
Size: 4157
NsiI A_TGCA'T
Cuts at: 427 427
Size: 4157
NspI r_CATG'y
Cuts at: 429 451 995 1210 1710 2617 429
Size: 22 544 215 500 907 1969
Fragments arranged by size:
1969 907 544 500 215 22
PciI A'CATG_T
Cuts at: 991 2613 991
Size: 1622 2535
PflMI CCAn_nnn'nTGG
Cuts at: 949 949
Size: 4157
PfoI T'CCnGG_A
Cuts at: 323 323
Size: 4157
PmeI GTTT'AAAC
Cuts at: 1156 1156
Size: 4157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3296 3328 3296
Size: 32 4125
PstI C_TGCA'G
Cuts at: 792 1204 1889 792
Size: 412 685 3060
Fragments arranged by size:
3060 685 412
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4157
PvuII CAG'CTG
Cuts at: 675 722 1582 1942 675
Size: 47 860 360 2890
Fragments arranged by size:
2890 860 360 47
RsrII CG'GwC_CG
Cuts at: 2352 2352
Size: 4157
SacI G_AGCT'C
Cuts at: 167 1171 167
Size: 1004 3153
SalI G'TCGA_C
Cuts at: 1194 1194
Size: 4157
SapI GCTCTTCn'nnn_
Cuts at: 2180 2390 2180
Size: 210 3947
SbfI CC_TGCA'GG
Cuts at: 1204 1204
Size: 4157
ScaI AGT'ACT
Cuts at: 3674 3674
Size: 4157
SfcI C'TryA_G
Cuts at: 33 788 1200 1885 2878 3069 33
Size: 755 412 685 993 191 1121
Fragments arranged by size:
1121 993 755 685 412 191
SfoI GGC'GCC
Cuts at: 1589 1837 1589
Size: 248 3909
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
SmaI CCC'GGG
Cuts at: 1179 1179
Size: 4157
SmlI C'TyrA_G
Cuts at: 118 166 206 1312 2227 2719 2981 118
Size: 48 40 1106 915 492 262 1294
Fragments arranged by size:
1294 1106 915 492 262 48 40
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4157
SphI G_CATG'C
Cuts at: 1210 1710 1210
Size: 500 3657
SspI AAT'ATT
Cuts at: 147 1012 147
Size: 865 3292
StyI C'CwwG_G
Cuts at: 626 704 762 1291 1645 626
Size: 78 58 529 354 3138
Fragments arranged by size:
3138 529 354 78 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1740 1873 2332 2541 1740
Size: 133 459 209 3356
Fragments arranged by size:
3356 459 209 133
TatI w'GTAC_w
Cuts at: 428 452 650 739 994 3672 428
Size: 24 198 89 255 2678 913
Fragments arranged by size:
2678 913 255 198 89 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 4125
Tth111I GACn'n_nGTC
Cuts at: 1954 1954
Size: 4157
XbaI T'CTAG_A
Cuts at: 1042 1188 1042
Size: 146 4011
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 496 1652 496
Size: 1156 3001
XmaI C'CCGG_G
Cuts at: 1177 1177
Size: 4157
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI