Restriction map for
pF1KB4535
(Circular) (Six-base) MAPSORT of: pF1KB4535.seq Check: 9674 from: 1 to: 4451
pF1KB4535 4451 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 532 966 532
Size: 434 4017
AccI GT'mk_AC
Cuts at: 1489 1489
Size: 4451
Acc65I G'GTAC_C
Cuts at: 1467 1467
Size: 4451
AclI AA'CG_TT
Cuts at: 4291 4291
Size: 4451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 1183 1329 2276 2708 3455 3723 613
Size: 570 146 947 432 747 268 1341
Fragments arranged by size:
1341 947 747 570 432 268 146
AfeI AGC'GCT
Cuts at: 659 1048 659
Size: 389 4062
AflIII A'CryG_T
Cuts at: 308 1803 2907 3884 308
Size: 1495 1104 977 875
Fragments arranged by size:
1495 1104 977 875
AgeI A'CCGG_T
Cuts at: 2839 2839
Size: 4451
AleI CACnn'nnGTG
Cuts at: 1027 1388 1027
Size: 361 4090
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 262 262
Size: 4451
AlwNI CAG_nnn'CTG
Cuts at: 435 1756 3323 435
Size: 1321 1567 1563
Fragments arranged by size:
1567 1563 1321
ApaLI G'TGCA_C
Cuts at: 3221 3221
Size: 4451
ApoI r'AATT_y
Cuts at: 1455 3804 4060 1455
Size: 2349 256 1846
Fragments arranged by size:
2349 1846 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4451
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
AvaI C'yCGr_G
Cuts at: 229 758 1285 1471 229
Size: 529 527 186 3209
Fragments arranged by size:
3209 529 527 186
BamHI G'GATC_C
Cuts at: 1476 1476
Size: 4451
BanI G'GyrC_C
Cuts at: 126 182 293 530 1356 1467 1881 2129 2164
Size: 56 111 237 826 111 414 248 35
Cuts at: 2164 126
Size: 2413
Fragments arranged by size:
2413 826 414 248 237 111 111 56
35
BanII G_rGCy'C
Cuts at: 1295 1313 1465 2495 1295
Size: 18 152 1030 3251
Fragments arranged by size:
3251 1030 152 18
BbeI G_GCGC'C
Cuts at: 186 297 534 1885 2133 186
Size: 111 237 1351 248 2504
Fragments arranged by size:
2504 1351 248 237 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 492 526 492
Size: 34 4417
BciVI GTATCCnnnnn_n'
Cuts at: 2349 3110 4451 2349
Size: 761 1341 2349
Fragments arranged by size:
2349 1341 761
BclI T'GATC_A
Cuts at: 1971 1971
Size: 4451
BglI GCCn_nnn'nGGC
Cuts at: 781 781
Size: 4451
BglII A'GATC_T
Cuts at: 1966 4003 1966
Size: 2037 2414
BlpI GC'TnA_GC
Cuts at: 1563 1563
Size: 4451
Bme1580I G_kGCm'C
Cuts at: 131 422 1361 1442 2076 2169 3225 131
Size: 291 939 81 634 93 1056 1357
Fragments arranged by size:
1357 1056 939 634 291 93 81
BmgBI CAC'GTC
Cuts at: 309 966 309
Size: 657 3794
BmrI ACTGGGnnnn_n'
Cuts at: 1663 1744 2079 4223 1663
Size: 81 335 2144 1891
Fragments arranged by size:
2144 1891 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2818 2818
Size: 4451
Bpu10I CC'TnA_GC
Cuts at: 581 581
Size: 4451
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1627 2506 2998 3296 1627
Size: 879 492 298 2782
Fragments arranged by size:
2782 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 487 755 4155 487
Size: 268 3400 783
Fragments arranged by size:
3400 783 268
BsaAI yAC'GTr
Cuts at: 1804 1804
Size: 4451
BsaBI GATnn'nnATC
Cuts at: 6 1965 4002 6
Size: 1959 2037 455
Fragments arranged by size:
2037 1959 455
BsaHI Gr'CG_yC
Cuts at: 183 294 334 462 531 1882 2130 2832 4328
Size: 111 40 128 69 1351 248 702 1496
Cuts at: 4328 183
Size: 306
Fragments arranged by size:
1496 1351 702 306 248 128 111 69
40
BsaWI w'CCGG_w
Cuts at: 927 1640 2161 2839 3113 3260 3671 927
Size: 713 521 678 274 147 411 1707
Fragments arranged by size:
1707 713 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 613 613
Size: 4451
BseYI C'CCAG_C
Cuts at: 191 234 654 701 744 749 1378 1876 2634
Size: 43 420 47 43 5 629 498 758
Cuts at: 2634 3211 3707 191
Size: 577 496 935
Fragments arranged by size:
935 758 629 577 498 496 420 47
43 43 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 955 1063 182
Size: 773 108 3570
Fragments arranged by size:
3570 773 108
BsiEI CG_ry'CG
Cuts at: 104 365 408 1993 2039 3247 104
Size: 261 43 1585 46 1208 1308
Fragments arranged by size:
1585 1308 1208 261 46 43
BsiHKAI G_wGCw'C
Cuts at: 350 728 1313 1465 2243 2433 3225 350
Size: 378 585 152 778 190 792 1576
Fragments arranged by size:
1576 792 778 585 378 190 152
BsmI GAATG_Cn'
Cuts at: 633 633
Size: 4451
Bsp1286I G_dGCh'C
Cuts at: 131 350 422 728 1295 1313 1361 1442 1465
Size: 219 72 306 567 18 48 81 23
Cuts at: 1465 2076 2169 2243 2433 2495 3225 131
Size: 611 93 74 190 62 730 1357
Fragments arranged by size:
1357 730 611 567 306 219 190 93
81 74 72 62 48 23 18
BspEI T'CCGG_A
Cuts at: 927 3671 927
Size: 2744 1707
BspHI T'CATG_A
Cuts at: 914 1035 3627 914
Size: 121 2592 1738
Fragments arranged by size:
2592 1738 121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 251 532 966 1501 1715 2017 2398 251
Size: 281 434 535 214 302 381 2304
Fragments arranged by size:
2304 535 434 381 302 281 214
BsrBI CCG'CTC
Cuts at: 50 2743 2797 4281 4446 50
Size: 2693 54 1484 165 55
Fragments arranged by size:
2693 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2363 2363
Size: 4451
BsrFI r'CCGG_y
Cuts at: 179 336 2449 2630 2839 179
Size: 157 2113 181 209 1791
Fragments arranged by size:
2113 1791 209 181 157
BssSI C'ACGA_G
Cuts at: 2722 3080 2722
Size: 358 4093
BstAPI GCAn_nnn'nTGC
Cuts at: 1828 1828
Size: 4451
BstBI TT'CG_AA
Cuts at: 2812 2812
Size: 4451
BstEII G'GTnAC_C
Cuts at: 381 876 997 1407 381
Size: 495 121 410 3425
Fragments arranged by size:
3425 495 410 121
BstXI CCAn_nnnn'nTGG
Cuts at: 144 144
Size: 4451
BstYI r'GATC_y
Cuts at: 1 431 1476 1943 1966 2301 2547 3559 3645
Size: 430 1045 467 23 335 246 1012 86
Cuts at: 3645 3657 4003 1
Size: 12 346 449
Fragments arranged by size:
1045 1012 467 449 430 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 562 1124 562
Size: 562 3889
BtgI C'CryG_G
Cuts at: 107 387 1386 107
Size: 280 999 3172
Fragments arranged by size:
3172 999 280
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2401 2582 2745 2401
Size: 181 163 4107
Fragments arranged by size:
4107 181 163
BtsI GCAGTG_nn'
Cuts at: 223 1022 1694 223
Size: 799 672 2980
Fragments arranged by size:
2980 799 672
DraI TTT'AAA
Cuts at: 1450 1450
Size: 4451
DraIII CAC_nnn'GTG
Cuts at: 1236 1236
Size: 4451
DrdI GACnn_nn'nnGTC
Cuts at: 2157 3015 2157
Size: 858 3593
EaeI y'GGCC_r
Cuts at: 134 146 362 2036 2210 2601 2628 3973 134
Size: 12 216 1674 174 391 27 1345 612
Fragments arranged by size:
1674 1345 612 391 216 174 27 12
EagI C'GGCC_G
Cuts at: 362 2036 362
Size: 1674 2777
EarI CTCTTCn'nnn_
Cuts at: 1318 1324 2474 2684 1318
Size: 6 1150 210 3085
Fragments arranged by size:
3085 1150 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2659 2969 3115 4261 2659
Size: 310 146 1146 2849
Fragments arranged by size:
2849 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1311 1463 1311
Size: 152 4299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 1183 1329 2276 2708 3455 3723 613
Size: 570 146 947 432 747 268 1341
Fragments arranged by size:
1341 947 747 570 432 268 146
EcoNI CCTnn'n_nnAGG
Cuts at: 580 769 580
Size: 189 4262
EcoO109I rG'GnC_Cy
Cuts at: 214 672 741 903 1590 214
Size: 458 69 162 687 3075
Fragments arranged by size:
3075 687 458 162 69
EcoRI G'AATT_C
Cuts at: 1455 1455
Size: 4451
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4451
FspI TGC'GCA
Cuts at: 2232 2232
Size: 4451
HaeII r_GCGC'y
Cuts at: 186 297 379 534 661 942 1050 1885 2133
Size: 111 82 155 127 281 108 835 248
Cuts at: 2133 3155 4134 186
Size: 1022 979 503
Fragments arranged by size:
1022 979 835 503 281 248 155 127
111 108 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 847 879 909 941 2293 2323 2325 2355 847
Size: 32 30 32 1352 30 2 30 2943
Fragments arranged by size:
2943 1352 32 32 30 30 30 2
HincII GTy'rAC
Cuts at: 1490 1490
Size: 4451
KasI G'GCGC_C
Cuts at: 182 293 530 1881 2129 182
Size: 111 237 1351 248 2504
Fragments arranged by size:
2504 1351 248 237 111
KpnI G_GTAC'C
Cuts at: 1471 1471
Size: 4451
MluI A'CGCG_T
Cuts at: 3884 3884
Size: 4451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 972 3122 3306 972
Size: 2150 184 2117
Fragments arranged by size:
2150 2117 184
MscI TGG'CCA
Cuts at: 136 148 2212 136
Size: 12 2064 2375
Fragments arranged by size:
2375 2064 12
MslI CAynn'nnrTG
Cuts at: 142 847 929 1027 1388 2440 2567 142
Size: 705 82 98 361 1052 127 2026
Fragments arranged by size:
2026 1052 705 361 127 98 82
MspA1I CmG'CkG
Cuts at: 324 705 1562 1876 2236 3249 3494 324
Size: 381 857 314 360 1013 245 1281
Fragments arranged by size:
1281 1013 857 381 360 314 245
NaeI GCC'GGC
Cuts at: 2632 2632
Size: 4451
NarI GG'CG_CC
Cuts at: 183 294 531 1882 2130 183
Size: 111 237 1351 248 2504
Fragments arranged by size:
2504 1351 248 237 111
NcoI C'CATG_G
Cuts at: 107 1386 107
Size: 1279 3172
NdeI CA'TA_TG
Cuts at: 1082 1082
Size: 4451
NgoMIV G'CCGG_C
Cuts at: 2630 2630
Size: 4451
NspI r_CATG'y
Cuts at: 253 1014 1283 1504 2004 2911 253
Size: 761 269 221 500 907 1793
Fragments arranged by size:
1793 907 761 500 269 221
PasI CC'CwG_GG
Cuts at: 1068 1224 1068
Size: 156 4295
PciI A'CATG_T
Cuts at: 2907 2907
Size: 4451
PflMI CCAn_nnn'nTGG
Cuts at: 435 435
Size: 4451
PfoI T'CCnGG_A
Cuts at: 1214 1214
Size: 4451
PmeI GTTT'AAAC
Cuts at: 1450 1450
Size: 4451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 3590
Size: 32 4419
PpuMI rG'GwC_Cy
Cuts at: 672 741 672
Size: 69 4382
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 256 288 256
Size: 32 4419
PstI C_TGCA'G
Cuts at: 323 1340 1498 2183 323
Size: 1017 158 685 2591
Fragments arranged by size:
2591 1017 685 158
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4451
PvuII CAG'CTG
Cuts at: 324 705 1876 2236 324
Size: 381 1171 360 2539
Fragments arranged by size:
2539 1171 381 360
RsrII CG'GwC_CG
Cuts at: 2646 2646
Size: 4451
SacI G_AGCT'C
Cuts at: 1313 1465 1313
Size: 152 4299
SalI G'TCGA_C
Cuts at: 1488 1488
Size: 4451
SanDI GG'GwC_CC
Cuts at: 741 741
Size: 4451
SapI GCTCTTCn'nnn_
Cuts at: 1318 2474 2684 1318
Size: 1156 210 3085
Fragments arranged by size:
3085 1156 210
SbfI CC_TGCA'GG
Cuts at: 1498 1498
Size: 4451
ScaI AGT'ACT
Cuts at: 3968 3968
Size: 4451
SfcI C'TryA_G
Cuts at: 33 319 1336 1494 2179 3172 3363 33
Size: 286 1017 158 685 993 191 1121
Fragments arranged by size:
1121 1017 993 685 286 191 158
SfoI GGC'GCC
Cuts at: 184 295 532 1883 2131 184
Size: 111 237 1351 248 2504
Fragments arranged by size:
2504 1351 248 237 111
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
SgrAI Cr'CCGG_yG
Cuts at: 179 336 179
Size: 157 4294
SmaI CCC'GGG
Cuts at: 231 1473 231
Size: 1242 3209
SmlI C'TyrA_G
Cuts at: 1606 2521 3013 3275 1606
Size: 915 492 262 2782
Fragments arranged by size:
2782 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4451
SphI G_CATG'C
Cuts at: 1283 1504 2004 1283
Size: 221 500 3730
Fragments arranged by size:
3730 500 221
SrfI GCCC'GGGC
Cuts at: 231 231
Size: 4451
StuI AGG'CCT
Cuts at: 775 1249 775
Size: 474 3977
StyI C'CwwG_G
Cuts at: 107 456 800 1386 1585 1939 107
Size: 349 344 586 199 354 2619
Fragments arranged by size:
2619 586 354 349 344 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 442 985 1060 2034 2167 2626 2835 442
Size: 543 75 974 133 459 209 2058
Fragments arranged by size:
2058 974 543 459 209 133 75
TatI w'GTAC_w
Cuts at: 1104 3966 1104
Size: 2862 1589
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3968 4000 3968
Size: 32 4419
Tth111I GACn'n_nGTC
Cuts at: 1060 2248 1060
Size: 1188 3263
XbaI T'CTAG_A
Cuts at: 1482 1482
Size: 4451
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 483 1946 483
Size: 1463 2988
XmaI C'CCGG_G
Cuts at: 229 1471 229
Size: 1242 3209
XmnI GAAnn'nnTTC
Cuts at: 1189 1189
Size: 4451
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BfrBI BmtI BpmI BsaXI BsiWI BsmBI
BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspOMI PspXI SacII SexAI SfiI
SnaBI SspI SwaI XhoI ZraI