Restriction map for
pF1KB4524
(Circular) (Six-base) MAPSORT of: pF1KB4524.seq Check: 2891 from: 1 to: 6929
pF1KB4524 6929 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1914 1914
Size: 6929
AccI GT'mk_AC
Cuts at: 3967 3967
Size: 6929
Acc65I G'GTAC_C
Cuts at: 3945 3945
Size: 6929
AclI AA'CG_TT
Cuts at: 987 6769 987
Size: 5782 1147
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 683 1057 1255 1559 1639 2857 4754 5186
Size: 433 374 198 304 80 1218 1897 432
Cuts at: 5186 5933 6201 250
Size: 747 268 978
Fragments arranged by size:
1897 1218 978 747 433 432 374 304
268 198 80
AflIII A'CryG_T
Cuts at: 4281 5385 6362 4281
Size: 1104 977 4848
Fragments arranged by size:
4848 1104 977
AgeI A'CCGG_T
Cuts at: 5317 5317
Size: 6929
AhdI GACnn_n'nnGTC
Cuts at: 3138 3138
Size: 6929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1712 1712
Size: 6929
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1079 1111 2360 2392 1079
Size: 32 1249 32 5616
Fragments arranged by size:
5616 1249 32 32
AlwNI CAG_nnn'CTG
Cuts at: 405 999 1716 4234 5801 405
Size: 594 717 2518 1567 1533
Fragments arranged by size:
2518 1567 1533 717 594
ApaI G_GGCC'C
Cuts at: 137 3761 137
Size: 3624 3305
ApaLI G'TGCA_C
Cuts at: 5699 5699
Size: 6929
ApoI r'AATT_y
Cuts at: 220 315 2204 3302 3330 3505 3933 6282 6538
Size: 95 1889 1098 28 175 428 2349 256
Cuts at: 6538 220
Size: 611
Fragments arranged by size:
2349 1889 1098 611 428 256 175 95
28
AseI AT'TA_AT
Cuts at: 20 1498 20
Size: 1478 5451
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6929
AvaI C'yCGr_G
Cuts at: 149 3949 149
Size: 3800 3129
BamHI G'GATC_C
Cuts at: 852 3954 852
Size: 3102 3827
BanI G'GyrC_C
Cuts at: 1472 3945 4359 4607 4642 1472
Size: 2473 414 248 35 3759
Fragments arranged by size:
3759 2473 414 248 35
BanII G_rGCy'C
Cuts at: 137 175 532 3761 3943 4973 137
Size: 38 357 3229 182 1030 2093
Fragments arranged by size:
3229 2093 1030 357 182 38
BbeI G_GCGC'C
Cuts at: 4363 4611 4363
Size: 248 6681
BbsI GAAGACnn'nnnn_
Cuts at: 3703 3703
Size: 6929
BbvCI CC'TCA_GC
Cuts at: 2474 2474
Size: 6929
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1138 1172 1138
Size: 34 6895
BciVI GTATCCnnnnn_n'
Cuts at: 4827 5588 6929 4827
Size: 761 1341 4827
Fragments arranged by size:
4827 1341 761
BclI T'GATC_A
Cuts at: 1058 1503 4449 1058
Size: 445 2946 3538
Fragments arranged by size:
3538 2946 445
BfrBI ATG'CAT
Cuts at: 3429 3429
Size: 6929
BglII A'GATC_T
Cuts at: 1650 4444 6481 1650
Size: 2794 2037 2098
Fragments arranged by size:
2794 2098 2037
BlpI GC'TnA_GC
Cuts at: 592 887 4041 592
Size: 295 3154 3480
Fragments arranged by size:
3480 3154 295
Bme1580I G_kGCm'C
Cuts at: 137 2893 2900 3761 4554 4647 5703 137
Size: 2756 7 861 793 93 1056 1363
Fragments arranged by size:
2756 1363 1056 861 793 93 7
BmrI ACTGGGnnnn_n'
Cuts at: 2383 4141 4222 4557 6701 2383
Size: 1758 81 335 2144 2611
Fragments arranged by size:
2611 2144 1758 335 81
BmtI G_CTAG'C
Cuts at: 3808 3808
Size: 6929
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 204 2499 3679 5296 204
Size: 2295 1180 1617 1837
Fragments arranged by size:
2295 1837 1617 1180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 622 1462 2030 2138 2252 2764 3063 274
Size: 348 840 568 108 114 512 299 4140
Fragments arranged by size:
4140 840 568 512 348 299 114 108
Bpu10I CC'TnA_GC
Cuts at: 2474 2474
Size: 6929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3196 3910 4105 4984 5476 5774 3196
Size: 714 195 879 492 298 4351
Fragments arranged by size:
4351 879 714 492 298 195
BsaI GGTCTCn'nnnn_
Cuts at: 3436 6633 3436
Size: 3197 3732
BsaAI yAC'GTr
Cuts at: 3010 4282 3010
Size: 1272 5657
BsaBI GATnn'nnATC
Cuts at: 6 4443 6480 6
Size: 4437 2037 455
Fragments arranged by size:
4437 2037 455
BsaHI Gr'CG_yC
Cuts at: 4360 4608 5310 6806 4360
Size: 248 702 1496 4483
Fragments arranged by size:
4483 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2444 4118 4639 5317 5591 5738 6149 2444
Size: 1674 521 678 274 147 411 3224
Fragments arranged by size:
3224 1674 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 466 496 550 580 1809 1839 1914 1917 1944
Size: 30 54 30 1229 30 75 3 27
Cuts at: 1944 1947 2046 2076 466
Size: 3 99 30 5319
Fragments arranged by size:
5319 1229 99 75 54 30 30 30
30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 442 1919 1922 1925 1928 1931 1934 1937 1940
Size: 1477 3 3 3 3 3 3 3
Cuts at: 1940 1943 1946 1980 2132 2210 2243 2246 2285
Size: 3 3 34 152 78 33 3 39
Cuts at: 2285 3016 442
Size: 731 4355
Fragments arranged by size:
4355 1477 731 152 78 39 34 33
3 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 724 1733 3336 3459 4354 5112 5689 6185 724
Size: 1009 1603 123 895 758 577 496 1468
Fragments arranged by size:
1603 1468 1009 895 758 577 496 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1420 2458 3809 1420
Size: 1038 1351 4540
Fragments arranged by size:
4540 1351 1038
BsiEI CG_ry'CG
Cuts at: 104 141 4471 4517 5725 104
Size: 37 4330 46 1208 1308
Fragments arranged by size:
4330 1308 1208 46 37
BsiHKAI G_wGCw'C
Cuts at: 532 1848 3943 4721 4911 5703 532
Size: 1316 2095 778 190 792 1758
Fragments arranged by size:
2095 1758 1316 792 778 190
BsmI GAATG_Cn'
Cuts at: 1677 2249 2318 3075 3429 3681 3739 1677
Size: 572 69 757 354 252 58 4867
Fragments arranged by size:
4867 757 572 354 252 69 58
Bsp1286I G_dGCh'C
Cuts at: 137 175 532 1848 2893 2900 3761 3943 4554
Size: 38 357 1316 1045 7 861 182 611
Cuts at: 4554 4647 4721 4911 4973 5703 137
Size: 93 74 190 62 730 1363
Fragments arranged by size:
1363 1316 1045 861 730 611 357 190
182 93 74 62 38 7
BspEI T'CCGG_A
Cuts at: 2444 6149 2444
Size: 3705 3224
BspHI T'CATG_A
Cuts at: 702 6105 702
Size: 5403 1526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1914 3979 4193 4495 4876 1914
Size: 2065 214 302 381 3967
Fragments arranged by size:
3967 2065 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3633 5221 5275 6759 6924 50
Size: 3583 1588 54 1484 165 55
Fragments arranged by size:
3583 1588 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 355 959 4841 355
Size: 604 3882 2443
Fragments arranged by size:
3882 2443 604
BsrFI r'CCGG_y
Cuts at: 1469 4927 5108 5317 1469
Size: 3458 181 209 3081
Fragments arranged by size:
3458 3081 209 181
BsrGI T'GTAC_A
Cuts at: 494 494
Size: 6929
BssSI C'ACGA_G
Cuts at: 5200 5558 5200
Size: 358 6571
BstAPI GCAn_nnn'nTGC
Cuts at: 4306 4306
Size: 6929
BstBI TT'CG_AA
Cuts at: 5290 5290
Size: 6929
BstXI CCAn_nnnn'nTGG
Cuts at: 2469 3906 2469
Size: 1437 5492
BstYI r'GATC_y
Cuts at: 1 852 1650 3516 3954 4421 4444 4779 5025
Size: 851 798 1866 438 467 23 335 246
Cuts at: 5025 6037 6123 6135 6481 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1866 1012 851 798 467 449 438 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2081 2081
Size: 6929
BtgI C'CryG_G
Cuts at: 107 141 848 3833 107
Size: 34 707 2985 3203
Fragments arranged by size:
3203 2985 707 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3701 4879 5060 5223 3701
Size: 1178 181 163 5407
Fragments arranged by size:
5407 1178 181 163
BtsI GCAGTG_nn'
Cuts at: 1672 2987 4172 1672
Size: 1315 1185 4429
Fragments arranged by size:
4429 1315 1185
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1949 1984 2168 2203 3870 3905 1949
Size: 35 184 35 1667 35 4973
Fragments arranged by size:
4973 1667 184 35 35 35
DraI TTT'AAA
Cuts at: 3928 3928
Size: 6929
DrdI GACnn_nn'nnGTC
Cuts at: 4635 5493 4635
Size: 858 6071
EaeI y'GGCC_r
Cuts at: 138 3912 4514 4688 5079 5106 6451 138
Size: 3774 602 174 391 27 1345 616
Fragments arranged by size:
3774 1345 616 602 391 174 27
EagI C'GGCC_G
Cuts at: 138 4514 138
Size: 4376 2553
EarI CTCTTCn'nnn_
Cuts at: 710 1211 2112 2270 2935 3646 3745 4952 5162
Size: 501 901 158 665 711 99 1207 210
Cuts at: 5162 710
Size: 2477
Fragments arranged by size:
2477 1207 901 711 665 501 210 158
99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5137 5447 5593 6739 5137
Size: 310 146 1146 5327
Fragments arranged by size:
5327 1146 310 146
EcoICRI GAG'CTC
Cuts at: 530 3941 530
Size: 3411 3518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 274 622 683 1057 1255 1462 1559 1639
Size: 24 348 61 374 198 207 97 80
Cuts at: 1639 2030 2138 2252 2764 2857 3063 4754 5186
Size: 391 108 114 512 93 206 1691 432
Cuts at: 5186 5933 6201 250
Size: 747 268 978
Fragments arranged by size:
1691 978 747 512 432 391 374 348
268 207 206 198 114 108 97 93
80 61 24
EcoNI CCTnn'n_nnAGG
Cuts at: 424 2485 3451 424
Size: 2061 966 3902
Fragments arranged by size:
3902 2061 966
EcoO109I rG'GnC_Cy
Cuts at: 133 146 1152 1713 2311 2478 3757 3758 4068
Size: 13 1006 561 598 167 1279 1 310
Cuts at: 4068 133
Size: 2994
Fragments arranged by size:
2994 1279 1006 598 561 310 167 13
1
EcoRI G'AATT_C
Cuts at: 2204 3933 2204
Size: 1729 5200
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4948 4948
Size: 6929
FspI TGC'GCA
Cuts at: 4710 4710
Size: 6929
HaeII r_GCGC'y
Cuts at: 4363 4611 5633 6612 4363
Size: 248 1022 979 4680
Fragments arranged by size:
4680 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 466 498 550 582 1050 1082 1091 1123 2965
Size: 32 52 32 468 32 9 32 1842
Cuts at: 2965 2997 3124 3156 3693 3725 4771 4801 4803
Size: 32 127 32 537 32 1046 30 2
Cuts at: 4803 4833 466
Size: 30 2562
Fragments arranged by size:
2562 1842 1046 537 468 127 52 32
32 32 32 32 32 32 30 30
9 2
HincII GTy'rAC
Cuts at: 1583 3968 1583
Size: 2385 4544
HindIII A'AGCT_T
Cuts at: 773 1642 2419 2464 3124 773
Size: 869 777 45 660 4578
Fragments arranged by size:
4578 869 777 660 45
HpaI GTT'AAC
Cuts at: 1583 1583
Size: 6929
KasI G'GCGC_C
Cuts at: 4359 4607 4359
Size: 248 6681
KpnI G_GTAC'C
Cuts at: 3949 3949
Size: 6929
MluI A'CGCG_T
Cuts at: 6362 6362
Size: 6929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 791 1023 1550 2642 3036 3768 5600 5784 791
Size: 232 527 1092 394 732 1832 184 1936
Fragments arranged by size:
1936 1832 1092 732 527 394 232 184
MscI TGG'CCA
Cuts at: 4690 4690
Size: 6929
MslI CAynn'nnrTG
Cuts at: 1258 1264 3670 4918 5045 1258
Size: 6 2406 1248 127 3142
Fragments arranged by size:
3142 2406 1248 127 6
MspA1I CmG'CkG
Cuts at: 143 596 999 1056 1081 3340 4040 4354 4714
Size: 453 403 57 25 2259 700 314 360
Cuts at: 4714 5727 5972 143
Size: 1013 245 1100
Fragments arranged by size:
2259 1100 1013 700 453 403 360 314
245 57 25
NaeI GCC'GGC
Cuts at: 1471 5110 1471
Size: 3639 3290
NarI GG'CG_CC
Cuts at: 4360 4608 4360
Size: 248 6681
NcoI C'CATG_G
Cuts at: 107 848 3833 107
Size: 741 2985 3203
Fragments arranged by size:
3203 2985 741
NdeI CA'TA_TG
Cuts at: 330 330
Size: 6929
NgoMIV G'CCGG_C
Cuts at: 1469 5108 1469
Size: 3639 3290
NheI G'CTAG_C
Cuts at: 3804 3804
Size: 6929
NsiI A_TGCA'T
Cuts at: 3431 3431
Size: 6929
NspI r_CATG'y
Cuts at: 2715 2999 3982 4482 5389 2715
Size: 284 983 500 907 4255
Fragments arranged by size:
4255 983 907 500 284
PciI A'CATG_T
Cuts at: 5385 5385
Size: 6929
PfoI T'CCnGG_A
Cuts at: 2277 2304 2741 2277
Size: 27 437 6465
Fragments arranged by size:
6465 437 27
PmeI GTTT'AAAC
Cuts at: 3928 3928
Size: 6929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6068 6100 6068
Size: 32 6897
PpuMI rG'GwC_Cy
Cuts at: 146 1152 1713 2478 146
Size: 1006 561 765 4597
Fragments arranged by size:
4597 1006 765 561
PsiI TTA'TAA
Cuts at: 2426 2426
Size: 6929
PspOMI G'GGCC_C
Cuts at: 133 3757 133
Size: 3624 3305
PstI C_TGCA'G
Cuts at: 1352 1554 2385 3728 3976 4661 1352
Size: 202 831 1343 248 685 3620
Fragments arranged by size:
3620 1343 831 685 248 202
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6929
PvuII CAG'CTG
Cuts at: 596 1056 3340 4354 4714 596
Size: 460 2284 1014 360 2811
Fragments arranged by size:
2811 2284 1014 460 360
RsrII CG'GwC_CG
Cuts at: 5124 5124
Size: 6929
SacI G_AGCT'C
Cuts at: 532 3943 532
Size: 3411 3518
SacII CC_GC'GG
Cuts at: 144 144
Size: 6929
SalI G'TCGA_C
Cuts at: 3966 3966
Size: 6929
SanDI GG'GwC_CC
Cuts at: 146 146
Size: 6929
SapI GCTCTTCn'nnn_
Cuts at: 4952 5162 4952
Size: 210 6719
SbfI CC_TGCA'GG
Cuts at: 3976 3976
Size: 6929
ScaI AGT'ACT
Cuts at: 1396 3469 6446 1396
Size: 2073 2977 1879
Fragments arranged by size:
2977 2073 1879
SexAI A'CCwGG_T
Cuts at: 1872 1872
Size: 6929
SfcI C'TryA_G
Cuts at: 33 1051 1348 1550 1600 2381 3724 3972 4657
Size: 1018 297 202 50 781 1343 248 685
Cuts at: 4657 5650 5841 33
Size: 993 191 1121
Fragments arranged by size:
1343 1121 1018 993 781 685 297 248
202 191 50
SfoI GGC'GCC
Cuts at: 4361 4609 4361
Size: 248 6681
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6929
SmaI CCC'GGG
Cuts at: 151 3951 151
Size: 3800 3129
SmlI C'TyrA_G
Cuts at: 3211 3889 4084 4999 5491 5753 3211
Size: 678 195 915 492 262 4387
Fragments arranged by size:
4387 915 678 492 262 195
SnaBI TAC'GTA
Cuts at: 3010 3010
Size: 6929
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6929
SphI G_CATG'C
Cuts at: 2715 3982 4482 2715
Size: 1267 500 5162
Fragments arranged by size:
5162 1267 500
StyI C'CwwG_G
Cuts at: 107 503 848 3611 3821 3833 3851 3899 4063
Size: 396 345 2763 210 12 18 48 164
Cuts at: 4063 4417 107
Size: 354 2619
Fragments arranged by size:
2763 2619 396 354 345 210 164 48
18 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 633 4512 4645 5104 5313 633
Size: 3879 133 459 209 2249
Fragments arranged by size:
3879 2249 459 209 133
TatI w'GTAC_w
Cuts at: 494 1394 3467 6444 494
Size: 900 2073 2977 979
Fragments arranged by size:
2977 2073 979 900
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1324 1356 1878 1910 1917 1949 6446 6478 1324
Size: 32 522 32 7 32 4497 32 1775
Fragments arranged by size:
4497 1775 522 32 32 32 32 7
Tth111I GACn'n_nGTC
Cuts at: 2769 4726 2769
Size: 1957 4972
XbaI T'CTAG_A
Cuts at: 3714 3960 3714
Size: 246 6683
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1788 4424 1788
Size: 2636 4293
XmaI C'CCGG_G
Cuts at: 149 3949 149
Size: 3800 3129
XmnI GAAnn'nnTTC
Cuts at: 318 1286 318
Size: 968 5961
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NsiI NspI PciI PfoI PmeI PpiI PpuMI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AscI AvrII BaeI BglI
BmgBI BsiWI BsmBI BssHII BstEII BstZ17I ClaI DraIII
EcoRV FseI FspAI MfeI NotI NruI PacI PasI
PflMI PmlI PshAI PspXI PsrI SfiI SgrAI SrfI
SspI StuI SwaI XhoI ZraI