Restriction map for
pF1KB4505
(Circular) (Six-base) MAPSORT of: pF1KB4505.seq Check: 3742 from: 1 to: 6629
pF1KB4505 6629 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 561 662 1177 1357 3228 3369 561
Size: 101 515 180 1871 141 3821
Fragments arranged by size:
3821 1871 515 180 141 101
AatII G_ACGT'C
Cuts at: 533 1337 533
Size: 804 5825
AccI GT'mk_AC
Cuts at: 2561 3667 2561
Size: 1106 5523
Acc65I G'GTAC_C
Cuts at: 3645 3645
Size: 6629
AclI AA'CG_TT
Cuts at: 6469 6469
Size: 6629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 696 709 2103 2967 3120 4454 4886 5633 5901
Size: 13 1394 864 153 1334 432 747 268
Cuts at: 5901 696
Size: 1424
Fragments arranged by size:
1424 1394 1334 864 747 432 268 153
13
AflIII A'CryG_T
Cuts at: 1055 1126 1294 3981 5085 6062 1055
Size: 71 168 2687 1104 977 1622
Fragments arranged by size:
2687 1622 1104 977 168 71
AgeI A'CCGG_T
Cuts at: 300 5017 300
Size: 4717 1912
AhdI GACnn_n'nnGTC
Cuts at: 390 390
Size: 6629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2477 2477
Size: 6629
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1066 1098 1066
Size: 32 6597
AlwNI CAG_nnn'CTG
Cuts at: 3934 5501 3934
Size: 1567 5062
ApaI G_GGCC'C
Cuts at: 251 424 877 1361 251
Size: 173 453 484 5519
Fragments arranged by size:
5519 484 453 173
ApaLI G'TGCA_C
Cuts at: 2452 2629 5399 2452
Size: 177 2770 3682
Fragments arranged by size:
3682 2770 177
ApoI r'AATT_y
Cuts at: 1141 2280 3633 5982 6238 1141
Size: 1139 1353 2349 256 1532
Fragments arranged by size:
2349 1532 1353 1139 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6629
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6629
AvaI C'yCGr_G
Cuts at: 895 948 1661 1785 3649 895
Size: 53 713 124 1864 3875
Fragments arranged by size:
3875 1864 713 124 53
BamHI G'GATC_C
Cuts at: 1646 3009 3654 1646
Size: 1363 645 4621
Fragments arranged by size:
4621 1363 645
BanI G'GyrC_C
Cuts at: 229 502 1570 1861 1926 3443 3645 4059 4307
Size: 273 1068 291 65 1517 202 414 248
Cuts at: 4307 4342 229
Size: 35 2516
Fragments arranged by size:
2516 1517 1068 414 291 273 248 202
65 35
BanII G_rGCy'C
Cuts at: 251 424 641 877 947 956 1028 1361 1603
Size: 173 217 236 70 9 72 333 242
Cuts at: 1603 1794 2051 2174 3643 4673 251
Size: 191 257 123 1469 1030 2207
Fragments arranged by size:
2207 1469 1030 333 257 242 236 217
191 173 123 72 70 9
BbeI G_GCGC'C
Cuts at: 233 1574 4063 4311 233
Size: 1341 2489 248 2551
Fragments arranged by size:
2551 2489 1341 248
BbsI GAAGACnn'nnnn_
Cuts at: 1439 2300 2523 3561 1439
Size: 861 223 1038 4507
Fragments arranged by size:
4507 1038 861 223
BbvCI CC'TCA_GC
Cuts at: 319 319
Size: 6629
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3160 3194 3160
Size: 34 6595
BciVI GTATCCnnnnn_n'
Cuts at: 4527 5288 6629 4527
Size: 761 1341 4527
Fragments arranged by size:
4527 1341 761
BclI T'GATC_A
Cuts at: 4149 4149
Size: 6629
BglI GCCn_nnn'nGGC
Cuts at: 791 1844 2153 2758 3059 791
Size: 1053 309 605 301 4361
Fragments arranged by size:
4361 1053 605 309 301
BglII A'GATC_T
Cuts at: 4144 6181 4144
Size: 2037 4592
BlpI GC'TnA_GC
Cuts at: 976 3741 976
Size: 2765 3864
Bme1580I G_kGCm'C
Cuts at: 251 403 424 877 1361 1514 1893 2456 2633
Size: 152 21 453 484 153 379 563 177
Cuts at: 2633 4254 4347 5403 251
Size: 1621 93 1056 1477
Fragments arranged by size:
1621 1477 1056 563 484 453 379 177
153 152 93 21
BmgBI CAC'GTC
Cuts at: 960 1295 2652 960
Size: 335 1357 4937
Fragments arranged by size:
4937 1357 335
BmrI ACTGGGnnnn_n'
Cuts at: 1275 1501 1788 3051 3264 3841 3922 4257 6401
Size: 226 287 1263 213 577 81 335 2144
Cuts at: 6401 1275
Size: 1503
Fragments arranged by size:
2144 1503 1263 577 335 287 226 213
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 471 4996 471
Size: 4525 2104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 436 544 1446 1617 135
Size: 301 108 902 171 5147
Fragments arranged by size:
5147 902 301 171 108
Bpu10I CC'TnA_GC
Cuts at: 319 1679 319
Size: 1360 5269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 973 3286 3560 3586 3805 4684 5176 5474
Size: 612 2313 274 26 219 879 492 298
Cuts at: 5474 361
Size: 1516
Fragments arranged by size:
2313 1516 879 612 492 298 274 219
26
BsaI GGTCTCn'nnnn_
Cuts at: 433 522 577 1265 1546 6333 433
Size: 89 55 688 281 4787 729
Fragments arranged by size:
4787 729 688 281 89 55
BsaAI yAC'GTr
Cuts at: 600 681 870 1127 2451 3153 3982 600
Size: 81 189 257 1324 702 829 3247
Fragments arranged by size:
3247 1324 829 702 257 189 81
BsaBI GATnn'nnATC
Cuts at: 6 4143 6180 6
Size: 4137 2037 455
Fragments arranged by size:
4137 2037 455
BsaHI Gr'CG_yC
Cuts at: 230 530 1334 1571 2498 2789 2819 4060 4308
Size: 300 804 237 927 291 30 1241 248
Cuts at: 4308 5010 6506 230
Size: 702 1496 353
Fragments arranged by size:
1496 1241 927 804 702 353 300 291
248 237 30
BsaWI w'CCGG_w
Cuts at: 300 3818 4339 5017 5291 5438 5849 300
Size: 3518 521 678 274 147 411 1080
Fragments arranged by size:
3518 1080 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1243 1273 2488 2518 1243
Size: 30 1215 30 5354
Fragments arranged by size:
5354 1215 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 903 934 1264 1758 2371 903
Size: 31 330 494 613 5161
Fragments arranged by size:
5161 613 494 330 31
BseYI C'CCAG_C
Cuts at: 148 371 877 924 1310 3555 4054 4812 5389
Size: 223 506 47 386 2245 499 758 577
Cuts at: 5389 5885 148
Size: 496 892
Fragments arranged by size:
2245 892 758 577 506 499 496 386
223 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 1104 1608 1738 1818 589
Size: 515 504 130 80 5400
Fragments arranged by size:
5400 515 504 130 80
BsiEI CG_ry'CG
Cuts at: 104 4171 4217 5425 104
Size: 4067 46 1208 1308
Fragments arranged by size:
4067 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 653 947 1028 1288 2456 2633 3338 3643 4421
Size: 294 81 260 1168 177 705 305 778
Cuts at: 4421 4611 5403 653
Size: 190 792 1879
Fragments arranged by size:
1879 1168 792 778 705 305 294 260
190 177 81
BsmI GAATG_Cn'
Cuts at: 1219 3616 1219
Size: 2397 4232
BsmBI CGTCTCn'nnnn_
Cuts at: 459 826 1498 2768 459
Size: 367 672 1270 4320
Fragments arranged by size:
4320 1270 672 367
Bsp1286I G_dGCh'C
Cuts at: 251 403 424 641 653 877 947 956 1028
Size: 152 21 217 12 224 70 9 72
Cuts at: 1028 1288 1361 1514 1603 1794 1893 2051 2174
Size: 260 73 153 89 191 99 158 123
Cuts at: 2174 2456 2633 3338 3643 4254 4347 4421 4611
Size: 282 177 705 305 611 93 74 190
Cuts at: 4611 4673 5403 251
Size: 62 730 1477
Fragments arranged by size:
1477 730 705 611 305 282 260 224
217 191 190 177 158 153 152 123
99 93 89 74 73 72 70 62
21 12 9
BspEI T'CCGG_A
Cuts at: 5849 5849
Size: 6629
BspHI T'CATG_A
Cuts at: 5805 5805
Size: 6629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 561 662 1141 1177 1192 1357 3228 3369 3679
Size: 101 479 36 15 165 1871 141 310
Cuts at: 3679 3893 4195 4576 561
Size: 214 302 381 2614
Fragments arranged by size:
2614 1871 479 381 310 302 214 165
141 101 36 15
BsrBI CCG'CTC
Cuts at: 50 4921 4975 6459 6624 50
Size: 4871 54 1484 165 55
Fragments arranged by size:
4871 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1863 4541 1863
Size: 2678 3951
BsrFI r'CCGG_y
Cuts at: 264 300 1079 1322 1639 2843 3181 4627 4808
Size: 36 779 243 317 1204 338 1446 181
Cuts at: 4808 5017 264
Size: 209 1876
Fragments arranged by size:
1876 1446 1204 779 338 317 243 209
181 36
BsrGI T'GTAC_A
Cuts at: 2132 2132
Size: 6629
BssSI C'ACGA_G
Cuts at: 912 964 4900 5258 912
Size: 52 3936 358 2283
Fragments arranged by size:
3936 2283 358 52
BstAPI GCAn_nnn'nTGC
Cuts at: 1349 1532 4006 1349
Size: 183 2474 3972
Fragments arranged by size:
3972 2474 183
BstBI TT'CG_AA
Cuts at: 4990 4990
Size: 6629
BstEII G'GTnAC_C
Cuts at: 163 1436 3196 163
Size: 1273 1760 3596
Fragments arranged by size:
3596 1760 1273
BstYI r'GATC_y
Cuts at: 1 480 1646 3009 3654 4121 4144 4479 4725
Size: 479 1166 1363 645 467 23 335 246
Cuts at: 4725 5737 5823 5835 6181 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1363 1166 1012 645 479 467 449 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3340 3340
Size: 6629
BtgI C'CryG_G
Cuts at: 107 243 467 1264 1612 1754 2507 3320 107
Size: 136 224 797 348 142 753 813 3416
Fragments arranged by size:
3416 813 797 753 348 224 142 136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2333 3084 4579 4760 4923 2333
Size: 751 1495 181 163 4039
Fragments arranged by size:
4039 1495 751 181 163
BtsI GCAGTG_nn'
Cuts at: 556 1959 2247 3872 556
Size: 1403 288 1625 3313
Fragments arranged by size:
3313 1625 1403 288
ClaI AT'CG_AT
Cuts at: 1586 1586
Size: 6629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1524 1559 3012 3047 1524
Size: 35 1453 35 5106
Fragments arranged by size:
5106 1453 35 35
DraI TTT'AAA
Cuts at: 1146 3628 1146
Size: 2482 4147
DraIII CAC_nnn'GTG
Cuts at: 741 1127 1239 741
Size: 386 112 6131
Fragments arranged by size:
6131 386 112
DrdI GACnn_nn'nnGTC
Cuts at: 4335 5193 4335
Size: 858 5771
EaeI y'GGCC_r
Cuts at: 683 1077 1545 1590 2709 2778 4214 4388 4779
Size: 394 468 45 1119 69 1436 174 391
Cuts at: 4779 4806 6151 683
Size: 27 1345 1161
Fragments arranged by size:
1436 1345 1161 1119 468 394 391 174
69 45 27
EagI C'GGCC_G
Cuts at: 4214 4214
Size: 6629
EarI CTCTTCn'nnn_
Cuts at: 2389 4652 4862 2389
Size: 2263 210 4156
Fragments arranged by size:
4156 2263 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 676 4837 5147 5293 6439 676
Size: 4161 310 146 1146 866
Fragments arranged by size:
4161 1146 866 310 146
EcoICRI GAG'CTC
Cuts at: 945 1026 3641 945
Size: 81 2615 3933
Fragments arranged by size:
3933 2615 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 436 544 696 709 1446 1617 2103 2967
Size: 301 108 152 13 737 171 486 864
Cuts at: 2967 3120 4454 4886 5633 5901 135
Size: 153 1334 432 747 268 863
Fragments arranged by size:
1334 864 863 747 737 486 432 301
268 171 153 152 108 13
EcoO109I rG'GnC_Cy
Cuts at: 420 421 874 1307 1357 1358 1817 1875 3520
Size: 1 453 433 50 1 459 58 1645
Cuts at: 3520 3768 420
Size: 248 3281
Fragments arranged by size:
3281 1645 459 453 433 248 58 50
1 1
EcoRI G'AATT_C
Cuts at: 3633 3633
Size: 6629
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 372 4648 372
Size: 4276 2353
FspI TGC'GCA
Cuts at: 4410 4410
Size: 6629
HaeII r_GCGC'y
Cuts at: 233 1574 4063 4311 5333 6312 233
Size: 1341 2489 248 1022 979 550
Fragments arranged by size:
2489 1341 1022 979 550 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 376 408 791 823 1243 1275 2488 2520 3109
Size: 32 383 32 420 32 1213 32 589
Cuts at: 3109 3141 4471 4501 4503 4533 376
Size: 32 1330 30 2 30 2472
Fragments arranged by size:
2472 1330 1213 589 420 383 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1941 3668 1941
Size: 1727 4902
KasI G'GCGC_C
Cuts at: 229 1570 4059 4307 229
Size: 1341 2489 248 2551
Fragments arranged by size:
2551 2489 1341 248
KpnI G_GTAC'C
Cuts at: 3649 3649
Size: 6629
MluI A'CGCG_T
Cuts at: 6062 6062
Size: 6629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 482 1796 2771 5300 5484 482
Size: 1314 975 2529 184 1627
Fragments arranged by size:
2529 1627 1314 975 184
MscI TGG'CCA
Cuts at: 685 4390 685
Size: 3705 2924
MslI CAynn'nnrTG
Cuts at: 4618 4745 4618
Size: 127 6502
MspA1I CmG'CkG
Cuts at: 245 469 1118 1595 2432 3740 4054 4414 5427
Size: 224 649 477 837 1308 314 360 1013
Cuts at: 5427 5672 245
Size: 245 1202
Fragments arranged by size:
1308 1202 1013 837 649 477 360 314
245 224
NaeI GCC'GGC
Cuts at: 266 2845 3183 4810 266
Size: 2579 338 1627 2085
Fragments arranged by size:
2579 2085 1627 338
NarI GG'CG_CC
Cuts at: 230 1571 4060 4308 230
Size: 1341 2489 248 2551
Fragments arranged by size:
2551 2489 1341 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 6629
NgoMIV G'CCGG_C
Cuts at: 264 2843 3181 4808 264
Size: 2579 338 1627 2085
Fragments arranged by size:
2579 2085 1627 338
NspI r_CATG'y
Cuts at: 514 856 1059 3682 4182 5089 514
Size: 342 203 2623 500 907 2054
Fragments arranged by size:
2623 2054 907 500 342 203
PciI A'CATG_T
Cuts at: 1055 5085 1055
Size: 4030 2599
PflMI CCAn_nnn'nTGG
Cuts at: 113 1239 1588 2284 113
Size: 1126 349 696 4458
Fragments arranged by size:
4458 1126 696 349
PfoI T'CCnGG_A
Cuts at: 938 1649 938
Size: 711 5918
PmeI GTTT'AAAC
Cuts at: 3628 3628
Size: 6629
PmlI CAC'GTG
Cuts at: 600 1127 600
Size: 527 6102
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5768 5800 5768
Size: 32 6597
PpuMI rG'GwC_Cy
Cuts at: 1307 1817 1875 3520 1307
Size: 510 58 1645 4416
Fragments arranged by size:
4416 1645 510 58
PshAI GACnn'nnGTC
Cuts at: 2549 2549
Size: 6629
PspOMI G'GGCC_C
Cuts at: 247 420 873 1357 247
Size: 173 453 484 5519
Fragments arranged by size:
5519 484 453 173
PstI C_TGCA'G
Cuts at: 659 3676 4361 659
Size: 3017 685 2927
Fragments arranged by size:
3017 2927 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6629
PvuII CAG'CTG
Cuts at: 1118 4054 4414 1118
Size: 2936 360 3333
Fragments arranged by size:
3333 2936 360
RsrII CG'GwC_CG
Cuts at: 4824 4824
Size: 6629
SacI G_AGCT'C
Cuts at: 947 1028 3643 947
Size: 81 2615 3933
Fragments arranged by size:
3933 2615 81
SacII CC_GC'GG
Cuts at: 246 470 246
Size: 224 6405
SalI G'TCGA_C
Cuts at: 3666 3666
Size: 6629
SanDI GG'GwC_CC
Cuts at: 1307 1307
Size: 6629
SapI GCTCTTCn'nnn_
Cuts at: 4652 4862 4652
Size: 210 6419
SbfI CC_TGCA'GG
Cuts at: 659 3676 659
Size: 3017 3612
ScaI AGT'ACT
Cuts at: 6146 6146
Size: 6629
SfcI C'TryA_G
Cuts at: 33 588 655 3104 3435 3672 4357 5350 5541
Size: 555 67 2449 331 237 685 993 191
Cuts at: 5541 33
Size: 1121
Fragments arranged by size:
2449 1121 993 685 555 331 237 191
67
SfoI GGC'GCC
Cuts at: 231 1572 4061 4309 231
Size: 1341 2489 248 2551
Fragments arranged by size:
2551 2489 1341 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6629
SgrAI Cr'CCGG_yG
Cuts at: 300 300
Size: 6629
SmaI CCC'GGG
Cuts at: 1663 1787 3651 1663
Size: 124 1864 4641
Fragments arranged by size:
4641 1864 124
SmlI C'TyrA_G
Cuts at: 376 988 3301 3575 3601 3784 4699 5191 5453
Size: 612 2313 274 26 183 915 492 262
Cuts at: 5453 376
Size: 1552
Fragments arranged by size:
2313 1552 915 612 492 274 262 183
26
SnaBI TAC'GTA
Cuts at: 3153 3153
Size: 6629
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6629
SphI G_CATG'C
Cuts at: 3682 4182 3682
Size: 500 6129
StuI AGG'CCT
Cuts at: 1702 1702
Size: 6629
StyI C'CwwG_G
Cuts at: 107 1441 3005 3763 4117 107
Size: 1334 1564 758 354 2619
Fragments arranged by size:
2619 1564 1334 758 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 129 1095 1879 2371 2662 3040 4212 4345 4804
Size: 966 784 492 291 378 1172 133 459
Cuts at: 4804 5013 129
Size: 209 1745
Fragments arranged by size:
1745 1172 966 784 492 459 378 291
209 133
TatI w'GTAC_w
Cuts at: 2132 6144 2132
Size: 4012 2617
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6146 6178 6146
Size: 32 6597
Tth111I GACn'n_nGTC
Cuts at: 2527 4426 2527
Size: 1899 4730
XbaI T'CTAG_A
Cuts at: 3660 3660
Size: 6629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 867 2217 3541 4124 867
Size: 1350 1324 583 3372
Fragments arranged by size:
3372 1350 1324 583
XmaI C'CCGG_G
Cuts at: 1661 1785 3649 1661
Size: 124 1864 4641
Fragments arranged by size:
4641 1864 124
XmnI GAAnn'nnTTC
Cuts at: 764 764
Size: 6629
ZraI GAC'GTC
Cuts at: 531 1335 531
Size: 804 5825
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AflII AleI AscI AvrII BaeI BfrBI BmtI
BsiWI BssHII BstXI BstZ17I EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PsiI PspXI PsrI SexAI SfiI SrfI
SspI SwaI XhoI