Restriction map for
pF1KB4486
(Circular) (Six-base) MAPSORT of: pF1KB4486.seq Check: 6027 from: 1 to: 8168
pF1KB4486 8168 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2755 2755
Size: 8168
AccI GT'mk_AC
Cuts at: 675 3027 3900 4272 5206 675
Size: 2352 873 372 934 3637
Fragments arranged by size:
3637 2352 934 873 372
Acc65I G'GTAC_C
Cuts at: 2785 4405 5014 5184 2785
Size: 1620 609 170 5769
Fragments arranged by size:
5769 1620 609 170
AclI AA'CG_TT
Cuts at: 8008 8008
Size: 8168
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 4184 4534 5993 6425 7172 7440 793
Size: 3391 350 1459 432 747 268 1521
Fragments arranged by size:
3391 1521 1459 747 432 350 268
AflIII A'CryG_T
Cuts at: 5075 5520 6624 7601 5075
Size: 445 1104 977 5642
Fragments arranged by size:
5642 1104 977 445
AgeI A'CCGG_T
Cuts at: 6556 6556
Size: 8168
AhdI GACnn_n'nnGTC
Cuts at: 419 419
Size: 8168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4548 4934 4548
Size: 386 7782
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2198 2230 2549 2581 2654 2686 2198
Size: 32 319 32 73 32 7680
Fragments arranged by size:
7680 319 73 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 184 1276 5473 7040 184
Size: 1092 4197 1567 1312
Fragments arranged by size:
4197 1567 1312 1092
ApaI G_GGCC'C
Cuts at: 453 721 817 3875 453
Size: 268 96 3058 4746
Fragments arranged by size:
4746 3058 268 96
ApaLI G'TGCA_C
Cuts at: 6938 6938
Size: 8168
ApoI r'AATT_y
Cuts at: 350 768 1037 1352 2264 2840 5172 7521 7777
Size: 418 269 315 912 576 2332 2349 256
Cuts at: 7777 350
Size: 741
Fragments arranged by size:
2349 2332 912 741 576 418 315 269
256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8168
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8168
AvaI C'yCGr_G
Cuts at: 326 5188 326
Size: 4862 3306
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3375 3408 3375
Size: 33 8135
BamHI G'GATC_C
Cuts at: 557 2067 2646 2705 2973 5193 557
Size: 1510 579 59 268 2220 3532
Fragments arranged by size:
3532 2220 1510 579 268 59
BanI G'GyrC_C
Cuts at: 2767 2785 4405 5014 5184 5598 5846 5881 2767
Size: 18 1620 609 170 414 248 35 5054
Fragments arranged by size:
5054 1620 609 414 248 170 35 18
BanII G_rGCy'C
Cuts at: 453 721 817 1398 1710 1812 2489 2992 3875
Size: 268 96 581 312 102 677 503 883
Cuts at: 3875 4602 4739 5182 6212 453
Size: 727 137 443 1030 2409
Fragments arranged by size:
2409 1030 883 727 677 581 503 443
312 268 137 102 96
BbeI G_GCGC'C
Cuts at: 5602 5850 5602
Size: 248 7920
BbsI GAAGACnn'nnnn_
Cuts at: 415 799 2134 3322 3331 415
Size: 384 1335 1188 9 5252
Fragments arranged by size:
5252 1335 1188 384 9
BciVI GTATCCnnnnn_n'
Cuts at: 920 2180 2224 3367 6066 6827 8168 920
Size: 1260 44 1143 2699 761 1341 920
Fragments arranged by size:
2699 1341 1260 1143 920 761 44
BclI T'GATC_A
Cuts at: 5688 5688
Size: 8168
BfrBI ATG'CAT
Cuts at: 4911 4911
Size: 8168
BglI GCCn_nnn'nGGC
Cuts at: 321 831 1427 1475 1991 2385 2954 321
Size: 510 596 48 516 394 569 5535
Fragments arranged by size:
5535 596 569 516 510 394 48
BglII A'GATC_T
Cuts at: 5683 7720 5683
Size: 2037 6131
BlpI GC'TnA_GC
Cuts at: 5280 5280
Size: 8168
Bme1580I G_kGCm'C
Cuts at: 453 721 817 3875 5793 5886 6942 453
Size: 268 96 3058 1918 93 1056 1679
Fragments arranged by size:
3058 1918 1679 1056 268 96 93
BmrI ACTGGGnnnn_n'
Cuts at: 1990 4112 5380 5461 5796 7940 1990
Size: 2122 1268 81 335 2144 2218
Fragments arranged by size:
2218 2144 2122 1268 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6535 6535
Size: 8168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 527 905 923 1229 1253 1334 1457 1475
Size: 336 378 18 306 24 81 123 18
Cuts at: 1475 1568 1604 1694 1703 1796 1826 1973 2261
Size: 93 36 90 9 93 30 147 288
Cuts at: 2261 2447 2720 2801 3053 3089 3269 3464 3578
Size: 186 273 81 252 36 180 195 114
Cuts at: 3578 3905 4012 4157 4223 4304 4799 191
Size: 327 107 145 66 81 495 3560
Fragments arranged by size:
3560 495 378 336 327 306 288 273
252 195 186 180 147 145 123 114
107 93 93 90 81 81 81 66
36 36 30 24 18 18 9
Bpu10I CC'TnA_GC
Cuts at: 4935 4935
Size: 8168
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 4277 5139 5344 6223 6715 7013 446
Size: 3831 862 205 879 492 298 1601
Fragments arranged by size:
3831 1601 879 862 492 298 205
BsaI GGTCTCn'nnnn_
Cuts at: 1612 3065 3148 3885 3931 4057 4084 4373 7872
Size: 1453 83 737 46 126 27 289 3499
Cuts at: 7872 1612
Size: 1908
Fragments arranged by size:
3499 1908 1453 737 289 126 83 46
27
BsaAI yAC'GTr
Cuts at: 931 5521 931
Size: 4590 3578
BsaBI GATnn'nnATC
Cuts at: 6 2978 5682 7719 6
Size: 2972 2704 2037 455
Fragments arranged by size:
2972 2704 2037 455
BsaHI Gr'CG_yC
Cuts at: 5599 5847 6549 8045 5599
Size: 248 702 1496 5722
Fragments arranged by size:
5722 1496 702 248
BsaWI w'CCGG_w
Cuts at: 5357 5878 6556 6830 6977 7388 5357
Size: 521 678 274 147 411 6137
Fragments arranged by size:
6137 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1928 1958 3882 3912 4010 4040 4302 4332 1928
Size: 30 1924 30 98 30 262 30 5764
Fragments arranged by size:
5764 1924 262 98 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 4464 4464
Size: 8168
BseYI C'CCAG_C
Cuts at: 2087 5593 6351 6928 7424 2087
Size: 3506 758 577 496 2831
Fragments arranged by size:
3506 2831 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3826 4382 4820 4940 3826
Size: 556 438 120 7054
Fragments arranged by size:
7054 556 438 120
BsiEI CG_ry'CG
Cuts at: 104 4048 5710 5756 6964 104
Size: 3944 1662 46 1208 1308
Fragments arranged by size:
3944 1662 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1710 1719 1812 2286 2418 2718 5182 5960 6150
Size: 9 93 474 132 300 2464 778 190
Cuts at: 6150 6942 1710
Size: 792 2936
Fragments arranged by size:
2936 2464 792 778 474 300 190 132
93 9
BsmI GAATG_Cn'
Cuts at: 437 1697 437
Size: 1260 6908
Bsp1286I G_dGCh'C
Cuts at: 453 721 817 1398 1710 1719 1812 2286 2418
Size: 268 96 581 312 9 93 474 132
Cuts at: 2418 2489 2718 2992 3875 4602 4739 5182 5793
Size: 71 229 274 883 727 137 443 611
Cuts at: 5793 5886 5960 6150 6212 6942 453
Size: 93 74 190 62 730 1679
Fragments arranged by size:
1679 883 730 727 611 581 474 443
312 274 268 229 190 137 132 96
93 93 74 71 62 9
BspEI T'CCGG_A
Cuts at: 7388 7388
Size: 8168
BspHI T'CATG_A
Cuts at: 4904 7344 4904
Size: 2440 5728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2755 5218 5432 5734 6115 2755
Size: 2463 214 302 381 4808
Fragments arranged by size:
4808 2463 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6460 6514 7998 8163 50
Size: 6410 54 1484 165 55
Fragments arranged by size:
6410 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 486 4344 6080 486
Size: 3858 1736 2574
Fragments arranged by size:
3858 2574 1736
BsrFI r'CCGG_y
Cuts at: 136 4043 6166 6347 6556 136
Size: 3907 2123 181 209 1748
Fragments arranged by size:
3907 2123 1748 209 181
BsrGI T'GTAC_A
Cuts at: 4581 4581
Size: 8168
BssSI C'ACGA_G
Cuts at: 6439 6797 6439
Size: 358 7810
BstAPI GCAn_nnn'nTGC
Cuts at: 184 5545 184
Size: 5361 2807
BstBI TT'CG_AA
Cuts at: 6529 6529
Size: 8168
BstEII G'GTnAC_C
Cuts at: 898 1063 2113 2149 3133 898
Size: 165 1050 36 984 5933
Fragments arranged by size:
5933 1050 984 165 36
BstYI r'GATC_y
Cuts at: 1 557 1486 1624 1732 2067 2646 2705 2973
Size: 556 929 138 108 335 579 59 268
Cuts at: 2973 3411 4071 4326 5193 5660 5683 6018 6264
Size: 438 660 255 867 467 23 335 246
Cuts at: 6264 7276 7362 7374 7720 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 929 867 660 579 556 467 449
438 346 335 335 268 255 246 138
108 86 59 23 12
BstZ17I GTA'TAC
Cuts at: 676 3028 676
Size: 2352 5816
Bsu36I CC'TnA_GG
Cuts at: 749 3218 4145 4868 749
Size: 2469 927 723 4049
Fragments arranged by size:
4049 2469 927 723
BtgI C'CryG_G
Cuts at: 4410 4602 4410
Size: 192 7976
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6118 6299 6462 6118
Size: 181 163 7824
Fragments arranged by size:
7824 181 163
BtsI GCAGTG_nn'
Cuts at: 234 601 5411 234
Size: 367 4810 2991
Fragments arranged by size:
4810 2991 367
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 692 727 692
Size: 35 8133
DraI TTT'AAA
Cuts at: 5167 5167
Size: 8168
DrdI GACnn_nn'nnGTC
Cuts at: 2243 2456 3122 3158 4166 5874 6732 2243
Size: 213 666 36 1008 1708 858 3679
Fragments arranged by size:
3679 1708 1008 858 666 213 36
EaeI y'GGCC_r
Cuts at: 1026 3711 4041 4045 4251 5753 5927 6318 6345
Size: 2685 330 4 206 1502 174 391 27
Cuts at: 6345 7690 1026
Size: 1345 1504
Fragments arranged by size:
2685 1504 1502 1345 391 330 206 174
27 4
EagI C'GGCC_G
Cuts at: 4045 5753 4045
Size: 1708 6460
EarI CTCTTCn'nnn_
Cuts at: 4967 5149 6191 6401 4967
Size: 182 1042 210 6734
Fragments arranged by size:
6734 1042 210 182
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6376 6686 6832 7978 6376
Size: 310 146 1146 6566
Fragments arranged by size:
6566 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1708 1810 5180 1708
Size: 102 3370 4696
Fragments arranged by size:
4696 3370 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 527 793 905 923 1229 1253 1334 1457
Size: 336 266 112 18 306 24 81 123
Cuts at: 1457 1475 1568 1604 1694 1703 1796 1826 1973
Size: 18 93 36 90 9 93 30 147
Cuts at: 1973 2261 2447 2720 2801 3053 3089 3269 3464
Size: 288 186 273 81 252 36 180 195
Cuts at: 3464 3578 3905 4012 4157 4184 4223 4304 4534
Size: 114 327 107 145 27 39 81 230
Cuts at: 4534 4799 5993 6425 7172 7440 191
Size: 265 1194 432 747 268 919
Fragments arranged by size:
1194 919 747 432 336 327 306 288
273 268 266 265 252 230 195 186
180 147 145 123 114 112 107 93
93 90 81 81 81 39 36 36
30 27 24 18 18 9
EcoO109I rG'GnC_Cy
Cuts at: 313 322 385 449 450 457 538 547 637
Size: 9 63 64 1 7 81 9 90
Cuts at: 637 718 745 778 813 907 943 1288 1411
Size: 81 27 33 35 94 36 345 123
Cuts at: 1411 1468 1477 1579 1615 1915 1960 1969 2056
Size: 57 9 102 36 300 45 9 87
Cuts at: 2056 2272 2317 2449 2458 2539 2653 2677 2731
Size: 216 45 132 9 81 114 24 54
Cuts at: 2731 2812 2830 2956 3124 3169 3214 3232 3490
Size: 81 18 126 168 45 45 18 258
Cuts at: 3490 3589 3685 3811 3820 3853 3872 3880 3916
Size: 99 96 126 9 33 19 8 36
Cuts at: 3916 4114 4177 4279 4288 4450 4459 5307 313
Size: 198 63 102 9 162 9 848 3174
Fragments arranged by size:
3174 848 345 300 258 216 198 168
162 132 126 126 123 114 102 102
99 96 94 90 87 81 81 81
81 64 63 63 57 54 45 45
45 45 36 36 36 35 33 33
27 24 19 18 18 9 9 9
9 9 9 9 9 8 7 1
EcoRI G'AATT_C
Cuts at: 350 1037 2264 2840 5172 350
Size: 687 1227 576 2332 3346
Fragments arranged by size:
3346 2332 1227 687 576
EcoRV GAT'ATC
Cuts at: 631 1755 631
Size: 1124 7044
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2121 2434 6187 2121
Size: 313 3753 4102
Fragments arranged by size:
4102 3753 313
FseI GG_CCGG'CC
Cuts at: 4047 4047
Size: 8168
FspI TGC'GCA
Cuts at: 5949 5949
Size: 8168
HaeII r_GCGC'y
Cuts at: 5602 5850 6872 7851 5602
Size: 248 1022 979 5919
Fragments arranged by size:
5919 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 405 437 705 737 918 950 2799 2831 3882
Size: 32 268 32 181 32 1849 32 1051
Cuts at: 3882 3914 4196 4228 6010 6040 6042 6072 405
Size: 32 282 32 1782 30 2 30 2501
Fragments arranged by size:
2501 1849 1782 1051 282 268 181 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1591 2611 3664 5207 1591
Size: 1020 1053 1543 4552
Fragments arranged by size:
4552 1543 1053 1020
KasI G'GCGC_C
Cuts at: 5598 5846 5598
Size: 248 7920
KpnI G_GTAC'C
Cuts at: 2789 4409 5018 5188 2789
Size: 1620 609 170 5769
Fragments arranged by size:
5769 1620 609 170
MfeI C'AATT_G
Cuts at: 686 857 686
Size: 171 7997
MluI A'CGCG_T
Cuts at: 7601 7601
Size: 8168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2490 4333 6839 7023 2490
Size: 1843 2506 184 3635
Fragments arranged by size:
3635 2506 1843 184
MscI TGG'CCA
Cuts at: 1028 3713 4253 5929 1028
Size: 2685 540 1676 3267
Fragments arranged by size:
3267 2685 1676 540
MslI CAynn'nnrTG
Cuts at: 4530 4744 4916 6157 6284 4530
Size: 214 172 1241 127 6414
Fragments arranged by size:
6414 1241 214 172 127
MspA1I CmG'CkG
Cuts at: 3640 4138 4631 4819 5279 5593 5953 6966 7211
Size: 498 493 188 460 314 360 1013 245
Cuts at: 7211 3640
Size: 4597
Fragments arranged by size:
4597 1013 498 493 460 360 314 245
188
NaeI GCC'GGC
Cuts at: 138 4045 6349 138
Size: 3907 2304 1957
Fragments arranged by size:
3907 2304 1957
NarI GG'CG_CC
Cuts at: 5599 5847 5599
Size: 248 7920
NgoMIV G'CCGG_C
Cuts at: 136 4043 6347 136
Size: 3907 2304 1957
Fragments arranged by size:
3907 2304 1957
NsiI A_TGCA'T
Cuts at: 4913 4913
Size: 8168
NspI r_CATG'y
Cuts at: 4755 5079 5221 5721 6628 4755
Size: 324 142 500 907 6295
Fragments arranged by size:
6295 907 500 324 142
PasI CC'CwG_GG
Cuts at: 553 1921 1948 3076 3379 3868 553
Size: 1368 27 1128 303 489 4853
Fragments arranged by size:
4853 1368 1128 489 303 27
PciI A'CATG_T
Cuts at: 5075 6624 5075
Size: 1549 6619
PflMI CCAn_nnn'nTGG
Cuts at: 3673 3673
Size: 8168
PfoI T'CCnGG_A
Cuts at: 207 390 399 426 471 543 624 642 741
Size: 183 9 27 45 72 81 18 99
Cuts at: 741 912 1131 1233 1260 1302 1311 1362 1371
Size: 171 219 102 27 42 9 51 9
Cuts at: 1371 1620 1701 1728 1767 1839 2232 2259 2268
Size: 249 81 27 39 72 393 27 9
Cuts at: 2268 2277 2340 2463 2649 2658 2673 2736 2754
Size: 9 63 123 186 9 15 63 18
Cuts at: 2754 2961 3174 3237 3255 3468 3495 3540 3630
Size: 207 213 63 18 213 27 45 90
Cuts at: 3630 3699 3744 4092 4182 4239 4320 4329 207
Size: 69 45 348 90 57 81 9 4046
Fragments arranged by size:
4046 393 348 249 219 213 213 207
186 183 171 123 102 99 90 90
81 81 81 72 72 69 63 63
63 57 51 45 45 45 42 39
27 27 27 27 27 18 18 18
15 9 9 9 9 9 9 9
PmeI GTTT'AAAC
Cuts at: 5167 5167
Size: 8168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7307 7339 7307
Size: 32 8136
PpuMI rG'GwC_Cy
Cuts at: 385 457 538 547 637 745 778 943 1288
Size: 72 81 9 90 108 33 165 345
Cuts at: 1288 1615 1915 1969 2056 2272 2449 2458 2539
Size: 327 300 54 87 216 177 9 81
Cuts at: 2539 2653 2677 2731 2812 2830 3124 3169 3685
Size: 114 24 54 81 18 294 45 516
Cuts at: 3685 3811 3853 3880 3916 4114 4177 4279 4288
Size: 126 42 27 36 198 63 102 9
Cuts at: 4288 4450 4459 385
Size: 162 9 4094
Fragments arranged by size:
4094 516 345 327 300 294 216 198
177 165 162 126 114 108 102 90
87 81 81 81 72 63 54 54
45 42 36 33 27 24 18 9
9 9 9
PshAI GACnn'nnGTC
Cuts at: 4385 4385
Size: 8168
PspOMI G'GGCC_C
Cuts at: 449 717 813 3871 449
Size: 268 96 3058 4746
Fragments arranged by size:
4746 3058 268 96
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3002 3034 3384 3416 3002
Size: 32 350 32 7754
Fragments arranged by size:
7754 350 32 32
PstI C_TGCA'G
Cuts at: 180 229 1281 5215 5900 180
Size: 49 1052 3934 685 2448
Fragments arranged by size:
3934 2448 1052 685 49
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8168
PvuII CAG'CTG
Cuts at: 3640 4138 4631 4819 5593 5953 3640
Size: 498 493 188 774 360 5855
Fragments arranged by size:
5855 774 498 493 360 188
RsrII CG'GwC_CG
Cuts at: 6363 6363
Size: 8168
SacI G_AGCT'C
Cuts at: 1710 1812 5182 1710
Size: 102 3370 4696
Fragments arranged by size:
4696 3370 102
SalI G'TCGA_C
Cuts at: 5205 5205
Size: 8168
SanDI GG'GwC_CC
Cuts at: 778 1969 3811 778
Size: 1191 1842 5135
Fragments arranged by size:
5135 1842 1191
SapI GCTCTTCn'nnn_
Cuts at: 6191 6401 6191
Size: 210 7958
SbfI CC_TGCA'GG
Cuts at: 5215 5215
Size: 8168
ScaI AGT'ACT
Cuts at: 7685 7685
Size: 8168
SexAI A'CCwGG_T
Cuts at: 417 579 660 705 1593 1746 2052 2190 2208
Size: 162 81 45 888 153 306 138 18
Cuts at: 2208 2241 2613 2718 2862 3021 3120 3549 3657
Size: 33 372 105 144 159 99 429 108
Cuts at: 3657 3894 3903 4191 4302 417
Size: 237 9 288 111 4283
Fragments arranged by size:
4283 888 429 372 306 288 237 162
159 153 144 138 111 108 105 99
81 45 33 18 9
SfcI C'TryA_G
Cuts at: 33 176 225 1169 1277 2726 2915 3557 5040
Size: 143 49 944 108 1449 189 642 1483
Cuts at: 5040 5060 5211 5896 6889 7080 33
Size: 20 151 685 993 191 1121
Fragments arranged by size:
1483 1449 1121 993 944 685 642 191
189 151 143 108 49 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 321 1427 1475 1991 2385 2954 321
Size: 1106 48 516 394 569 5535
Fragments arranged by size:
5535 1106 569 516 394 48
SfoI GGC'GCC
Cuts at: 5600 5848 5600
Size: 248 7920
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8168
SmaI CCC'GGG
Cuts at: 5190 5190
Size: 8168
SmlI C'TyrA_G
Cuts at: 461 4292 5118 5323 6238 6730 6992 461
Size: 3831 826 205 915 492 262 1637
Fragments arranged by size:
3831 1637 915 826 492 262 205
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8168
SphI G_CATG'C
Cuts at: 4755 5221 5721 4755
Size: 466 500 7202
Fragments arranged by size:
7202 500 466
StuI AGG'CCT
Cuts at: 656 1943 3464 656
Size: 1287 1521 5360
Fragments arranged by size:
5360 1521 1287
StyI C'CwwG_G
Cuts at: 488 1509 3876 4284 5302 5656 488
Size: 1021 2367 408 1018 354 3000
Fragments arranged by size:
3000 2367 1021 1018 408 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 188 296 917 5751 5884 6343 6552 188
Size: 108 621 4834 133 459 209 1804
Fragments arranged by size:
4834 1804 621 459 209 133 108
TatI w'GTAC_w
Cuts at: 4581 7683 4581
Size: 3102 5066
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4010 4042 4870 4902 7685 7717 4010
Size: 32 828 32 2783 32 4461
Fragments arranged by size:
4461 2783 828 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3896 5965 3896
Size: 2069 6099
XbaI T'CTAG_A
Cuts at: 2310 5199 2310
Size: 2889 5279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2796 3198 3721 3883 4036 5663 2796
Size: 402 523 162 153 1627 5301
Fragments arranged by size:
5301 1627 523 402 162 153
XmaI C'CCGG_G
Cuts at: 5188 5188
Size: 8168
XmnI GAAnn'nnTTC
Cuts at: 4976 4976
Size: 8168
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AscI AvrII BbvCI BcgI
BmgBI BmtI BsiWI BsmBI BssHII BstXI ClaI DraIII
EcoNI FspAI HindIII HpaI NcoI NdeI NheI NotI
NruI PacI PmlI PsiI PspXI SacII SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI