Restriction map for
pF1KB4465
(Circular) (Six-base) MAPSORT of: pF1KB4465.seq Check: 7388 from: 1 to: 10076
pF1KB4465 10076 bp
With 182 enzymes: SgfI *
May 1, 2012 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 634 748 1099 1316 1597 2392 2623 2842 2848
Size: 114 351 217 281 795 231 219 6
Cuts at: 2848 3076 3658 6648 634
Size: 228 582 2990 4062
Fragments arranged by size:
4062 2990 795 582 351 281 231 228
219 217 114 6
AatII G_ACGT'C
Cuts at: 2409 2409
Size: 10076
AccI GT'mk_AC
Cuts at: 7114 7114
Size: 10076
Acc65I G'GTAC_C
Cuts at: 973 7092 973
Size: 6119 3957
AclI AA'CG_TT
Cuts at: 9916 9916
Size: 10076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1563 1587 5552 7901 8333 9080 9348 1563
Size: 24 3965 2349 432 747 268 2291
Fragments arranged by size:
3965 2349 2291 747 432 268 24
AfeI AGC'GCT
Cuts at: 4306 4306
Size: 10076
AflIII A'CryG_T
Cuts at: 1615 3425 7428 8532 9509 1615
Size: 1810 4003 1104 977 2182
Fragments arranged by size:
4003 2182 1810 1104 977
AgeI A'CCGG_T
Cuts at: 8464 8464
Size: 10076
AleI CACnn'nnGTG
Cuts at: 1069 1948 3652 4487 6508 1069
Size: 879 1704 835 2021 4637
Fragments arranged by size:
4637 2021 1704 879 835
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 295 3018 4750 5660 5749 295
Size: 2723 1732 910 89 4622
Fragments arranged by size:
4622 2723 1732 910 89
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5012 5044 5012
Size: 32 10044
AlwNI CAG_nnn'CTG
Cuts at: 2304 2880 2903 3105 4430 5437 6931 7381 8948
Size: 576 23 202 1325 1007 1494 450 1567
Cuts at: 8948 2304
Size: 3432
Fragments arranged by size:
3432 1567 1494 1325 1007 576 450 202
23
ApaI G_GGCC'C
Cuts at: 123 521 1424 2063 3091 3629 4815 6197 6338
Size: 398 903 639 1028 538 1186 1382 141
Cuts at: 6338 6379 6449 6485 6627 7034 123
Size: 41 70 36 142 407 3165
Fragments arranged by size:
3165 1382 1186 1028 903 639 538 407
398 142 141 70 41 36
ApaLI G'TGCA_C
Cuts at: 8846 8846
Size: 10076
ApoI r'AATT_y
Cuts at: 7080 9429 9685 7080
Size: 2349 256 7471
Fragments arranged by size:
7471 2349 256
AseI AT'TA_AT
Cuts at: 20 3584 20
Size: 3564 6512
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10076
AvaI C'yCGr_G
Cuts at: 287 1596 2190 2309 2799 3073 3764 3900 4089
Size: 1309 594 119 490 274 691 136 189
Cuts at: 4089 4243 4808 4831 6307 6338 6372 7096 287
Size: 154 565 23 1476 31 34 724 3267
Fragments arranged by size:
3267 1476 1309 724 691 594 565 490
274 189 154 136 119 34 31 23
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3687 3720 3687
Size: 33 10043
BamHI G'GATC_C
Cuts at: 7101 7101
Size: 10076
BanI G'GyrC_C
Cuts at: 406 428 499 622 702 736 973 1300 1663
Size: 22 71 123 80 34 237 327 363
Cuts at: 1663 2803 3632 3646 3742 4227 4239 4265 4409
Size: 1140 829 14 96 485 12 26 144
Cuts at: 4409 4617 5394 6554 6675 7092 7506 7754 7789
Size: 208 777 1160 121 417 414 248 35
Cuts at: 7789 406
Size: 2693
Fragments arranged by size:
2693 1160 1140 829 777 485 417 414
363 327 248 237 208 144 123 121
96 80 71 35 34 26 22 14
12
BanII G_rGCy'C
Cuts at: 123 521 608 1348 1424 2063 2216 2716 2979
Size: 398 87 740 76 639 153 500 263
Cuts at: 2979 3091 3629 4153 4815 5514 5595 5882 6197
Size: 112 538 524 662 699 81 287 315
Cuts at: 6197 6338 6379 6449 6485 6627 7034 7090 8120
Size: 141 41 70 36 142 407 56 1030
Cuts at: 8120 123
Size: 2079
Fragments arranged by size:
2079 1030 740 699 662 639 538 524
500 407 398 315 287 263 153 142
141 112 87 81 76 70 56 41
36
BbeI G_GCGC'C
Cuts at: 1304 1667 4231 4413 6558 6679 7510 7758 1304
Size: 363 2564 182 2145 121 831 248 3622
Fragments arranged by size:
3622 2564 2145 831 363 248 182 121
BbsI GAAGACnn'nnnn_
Cuts at: 772 3305 3430 4764 5760 772
Size: 2533 125 1334 996 5088
Fragments arranged by size:
5088 2533 1334 996 125
BbvCI CC'TCA_GC
Cuts at: 478 5330 478
Size: 4852 5224
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 404 438 1042 1076 3054 3088 3507 3541 4525
Size: 34 604 34 1978 34 419 34 984
Cuts at: 4525 4559 5416 5450 404
Size: 34 857 34 5030
Fragments arranged by size:
5030 1978 984 857 604 419 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3688 7974 8735 10076 3688
Size: 4286 761 1341 3688
Fragments arranged by size:
4286 3688 1341 761
BclI T'GATC_A
Cuts at: 4899 7596 4899
Size: 2697 7379
BfrBI ATG'CAT
Cuts at: 3518 3518
Size: 10076
BglI GCCn_nnn'nGGC
Cuts at: 314 437 551 620 1133 1598 1886 1889 2015
Size: 123 114 69 513 465 288 3 126
Cuts at: 2015 2048 2105 2162 2507 2705 2849 2963 3329
Size: 33 57 57 345 198 144 114 366
Cuts at: 3329 4007 4035 4285 4630 5471 5815 5854 5974
Size: 678 28 250 345 841 344 39 120
Cuts at: 5974 6333 6342 6940 314
Size: 359 9 598 3450
Fragments arranged by size:
3450 841 678 598 513 465 366 359
345 345 344 288 250 198 144 126
123 120 114 114 69 57 57 39
33 28 9 3
BglII A'GATC_T
Cuts at: 7591 9628 7591
Size: 2037 8039
BlpI GC'TnA_GC
Cuts at: 4363 4711 5594 7188 4363
Size: 348 883 1594 7251
Fragments arranged by size:
7251 1594 883 348
Bme1580I G_kGCm'C
Cuts at: 123 310 433 495 504 521 707 739 895
Size: 187 123 62 9 17 186 32 156
Cuts at: 895 1424 1726 1837 2063 2208 2806 3091 3325
Size: 529 302 111 226 145 598 285 234
Cuts at: 3325 3629 3942 4003 4270 4815 5287 5607 6197
Size: 304 313 61 267 545 472 320 590
Cuts at: 6197 6338 6379 6449 6485 6614 6627 6786 6966
Size: 141 41 70 36 129 13 159 180
Cuts at: 6966 7034 7701 7794 8850 123
Size: 68 667 93 1056 1349
Fragments arranged by size:
1349 1056 667 598 590 545 529 472
320 313 304 302 285 267 234 226
187 186 180 159 156 145 141 129
123 111 93 70 68 62 61 41
36 32 17 13 9
BmgBI CAC'GTC
Cuts at: 582 930 1629 3474 4992 5163 582
Size: 348 699 1845 1518 171 5495
Fragments arranged by size:
5495 1845 1518 699 348 171
BmrI ACTGGGnnnn_n'
Cuts at: 3170 5949 6182 6818 6879 6947 6968 7288 7369
Size: 2779 233 636 61 68 21 320 81
Cuts at: 7369 7704 9848 3170
Size: 335 2144 3398
Fragments arranged by size:
3398 2779 2144 636 335 320 233 81
68 61 21
BmtI G_CTAG'C
Cuts at: 208 5564 208
Size: 5356 4720
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3504 3753 5163 8443 3504
Size: 249 1410 3280 5137
Fragments arranged by size:
5137 3280 1410 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 2298 3280 4879 5000 5029 5536 6227 6587
Size: 1937 982 1599 121 29 507 691 360
Cuts at: 6587 6632 361
Size: 45 3805
Fragments arranged by size:
3805 1937 1599 982 691 507 360 121
45 29
Bpu10I CC'TnA_GC
Cuts at: 478 2782 3738 5330 6863 7010 478
Size: 2304 956 1592 1533 147 3544
Fragments arranged by size:
3544 2304 1592 1533 956 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6496 7252 8131 8623 8921 6496
Size: 756 879 492 298 7651
Fragments arranged by size:
7651 879 756 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1387 4254 6019 9780 1387
Size: 2867 1765 3761 1683
Fragments arranged by size:
3761 2867 1765 1683
BsaAI yAC'GTr
Cuts at: 1442 1616 2820 7429 1442
Size: 174 1204 4609 4089
Fragments arranged by size:
4609 4089 1204 174
BsaBI GATnn'nnATC
Cuts at: 6 5779 7590 9627 6
Size: 5773 1811 2037 455
Fragments arranged by size:
5773 2037 1811 455
BsaHI Gr'CG_yC
Cuts at: 869 1166 1301 1664 2315 2406 3032 3198 4010
Size: 297 135 363 651 91 626 166 812
Cuts at: 4010 4027 4228 4410 6555 6676 7507 7755 8457
Size: 17 201 182 2145 121 831 248 702
Cuts at: 8457 9953 869
Size: 1496 992
Fragments arranged by size:
2145 1496 992 831 812 702 651 626
363 297 248 201 182 166 135 121
91 17
BsaWI w'CCGG_w
Cuts at: 1215 7265 7786 8464 8738 8885 9296 1215
Size: 6050 521 678 274 147 411 1995
Fragments arranged by size:
6050 1995 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1230 1260 1738 1768 2168 2198 2809 2839 3253
Size: 30 478 30 400 30 611 30 414
Cuts at: 3253 3283 3982 4012 4135 4165 4249 4279 6560
Size: 30 699 30 123 30 84 30 2281
Cuts at: 6560 6590 1230
Size: 30 4716
Fragments arranged by size:
4716 2281 699 611 478 414 400 123
84 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2410 4270 4594 4687 5326 5347 6037 6778 2410
Size: 1860 324 93 639 21 690 741 5708
Fragments arranged by size:
5708 1860 741 690 639 324 93 21
BseYI C'CCAG_C
Cuts at: 275 317 990 2327 2896 3377 3491 3564 3863
Size: 42 673 1337 569 481 114 73 299
Cuts at: 3863 3941 4340 4589 5023 5211 5689 6321 6686
Size: 78 399 249 434 188 478 632 365
Cuts at: 6686 6778 6924 6974 6997 7022 7026 7034 7501
Size: 92 146 50 23 25 4 8 467
Cuts at: 7501 8259 8836 9332 275
Size: 758 577 496 1019
Fragments arranged by size:
1337 1019 758 673 632 577 569 496
481 478 467 434 399 365 299 249
188 146 114 92 78 73 50 42
25 23 8 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 903 1569 1977 2355 2364 2415 2568 2595 2820
Size: 666 408 378 9 51 153 27 225
Cuts at: 2820 3048 3138 3663 5137 5719 6139 903
Size: 228 90 525 1474 582 420 4840
Fragments arranged by size:
4840 1474 666 582 525 420 408 378
228 225 153 90 51 27 9
BsiEI CG_ry'CG
Cuts at: 104 1227 1368 1452 4130 4314 4554 7618 7664
Size: 1123 141 84 2678 184 240 3064 46
Cuts at: 7664 8872 104
Size: 1208 1308
Fragments arranged by size:
3064 2678 1308 1208 1123 240 184 141
84 46
BsiHKAI G_wGCw'C
Cuts at: 1294 1319 1821 3575 4652 5120 5882 6833 6869
Size: 25 502 1754 1077 468 762 951 36
Cuts at: 6869 7090 7868 8058 8850 1294
Size: 221 778 190 792 2520
Fragments arranged by size:
2520 1754 1077 951 792 778 762 502
468 221 190 36 25
BsmI GAATG_Cn'
Cuts at: 1642 1867 1642
Size: 225 9851
BsmBI CGTCTCn'nnnn_
Cuts at: 2306 6717 2306
Size: 4411 5665
Bsp1286I G_dGCh'C
Cuts at: 123 310 433 495 504 521 608 707 739
Size: 187 123 62 9 17 87 99 32
Cuts at: 739 895 1294 1319 1348 1424 1726 1821 1837
Size: 156 399 25 29 76 302 95 16
Cuts at: 1837 2063 2208 2216 2716 2806 2979 3091 3325
Size: 226 145 8 500 90 173 112 234
Cuts at: 3325 3575 3629 3942 4003 4153 4270 4652 4815
Size: 250 54 313 61 150 117 382 163
Cuts at: 4815 5120 5287 5514 5595 5607 5882 6197 6338
Size: 305 167 227 81 12 275 315 141
Cuts at: 6338 6379 6449 6485 6614 6627 6786 6833 6869
Size: 41 70 36 129 13 159 47 36
Cuts at: 6869 6966 7034 7090 7701 7794 7868 8058 8120
Size: 97 68 56 611 93 74 190 62
Cuts at: 8120 8850 123
Size: 730 1349
Fragments arranged by size:
1349 730 611 500 399 382 315 313
305 302 275 250 234 227 226 190
187 173 167 163 159 156 150 145
141 129 123 117 112 99 97 95
93 90 87 81 76 74 70 68
62 62 61 56 54 47 41 36
36 32 29 25 17 16 13 12
9 8
BspEI T'CCGG_A
Cuts at: 9296 9296
Size: 10076
BspHI T'CATG_A
Cuts at: 9252 9252
Size: 10076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 634 748 985 1099 1316 1597 2392 2623 2842
Size: 114 237 114 217 281 795 231 219
Cuts at: 2842 2848 2888 3076 3409 3658 6648 7126 7340
Size: 6 40 188 333 249 2990 478 214
Cuts at: 7340 7642 8023 634
Size: 302 381 2687
Fragments arranged by size:
2990 2687 795 478 381 333 302 281
249 237 231 219 217 214 188 114
114 40 6
BsrBI CCG'CTC
Cuts at: 50 330 3194 3969 4170 5792 6726 8368 8422
Size: 280 2864 775 201 1622 934 1642 54
Cuts at: 8422 9906 10071 50
Size: 1484 165 55
Fragments arranged by size:
2864 1642 1622 1484 934 775 280 201
165 55 54
BsrDI GCAATG_nn'
Cuts at: 4393 7988 4393
Size: 3595 6481
BsrFI r'CCGG_y
Cuts at: 425 2341 2746 4486 4620 4784 4871 5183 8074
Size: 1916 405 1740 134 164 87 312 2891
Cuts at: 8074 8255 8464 425
Size: 181 209 2037
Fragments arranged by size:
2891 2037 1916 1740 405 312 209 181
164 134 87
BssHII G'CGCG_C
Cuts at: 3976 4196 4272 3976
Size: 220 76 9780
Fragments arranged by size:
9780 220 76
BssSI C'ACGA_G
Cuts at: 1189 2694 3526 8347 8705 1189
Size: 1505 832 4821 358 2560
Fragments arranged by size:
4821 2560 1505 832 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1184 1652 1841 1991 2792 6970 7453 1184
Size: 468 189 150 801 4178 483 3807
Fragments arranged by size:
4178 3807 801 483 468 189 150
BstBI TT'CG_AA
Cuts at: 8437 8437
Size: 10076
BstXI CCAn_nnnn'nTGG
Cuts at: 1674 1687 4489 6565 1674
Size: 13 2802 2076 5185
Fragments arranged by size:
5185 2802 2076 13
BstYI r'GATC_y
Cuts at: 1 468 7101 7568 7591 7926 8172 9184 9270
Size: 467 6633 467 23 335 246 1012 86
Cuts at: 9270 9282 9628 1
Size: 12 346 449
Fragments arranged by size:
6633 1012 467 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 5138 5138
Size: 10076
BtgI C'CryG_G
Cuts at: 107 123 375 435 495 615 698 1080 1130
Size: 16 252 60 60 120 83 382 50
Cuts at: 1130 1773 1884 2619 3405 4032 4143 4235 4261
Size: 643 111 735 786 627 111 92 26
Cuts at: 4261 4635 4766 5195 5402 6130 6216 6410 6430
Size: 374 131 429 207 728 86 194 20
Cuts at: 6430 6944 107
Size: 514 3239
Fragments arranged by size:
3239 786 735 728 643 627 514 429
382 374 252 207 194 131 120 111
111 92 86 83 60 60 50 26
20 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1779 2375 2780 5868 8026 8207 8370 1779
Size: 596 405 3088 2158 181 163 3485
Fragments arranged by size:
3485 3088 2158 596 405 181 163
BtsI GCAGTG_nn'
Cuts at: 2376 2865 2979 3100 3292 4003 4408 4902 4981
Size: 489 114 121 192 711 405 494 79
Cuts at: 4981 5041 5149 6497 6503 6515 6929 6964 7319
Size: 60 108 1348 6 12 414 35 355
Cuts at: 7319 2376
Size: 5133
Fragments arranged by size:
5133 1348 711 494 489 414 405 355
192 121 114 108 79 60 35 12
6
ClaI AT'CG_AT
Cuts at: 1052 1052
Size: 10076
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 5442 5477 6968 7003 5442
Size: 35 1491 35 8515
Fragments arranged by size:
8515 1491 35 35
DraI TTT'AAA
Cuts at: 7075 7075
Size: 10076
DraIII CAC_nnn'GTG
Cuts at: 688 1730 2566 5282 688
Size: 1042 836 2716 5482
Fragments arranged by size:
5482 2716 1042 836
DrdI GACnn_nn'nnGTC
Cuts at: 839 1171 3846 6394 7782 8640 839
Size: 332 2675 2548 1388 858 2275
Fragments arranged by size:
2675 2548 2275 1388 858 332
EaeI y'GGCC_r
Cuts at: 126 372 996 1224 2478 4127 4491 4551 4758
Size: 246 624 228 1254 1649 364 60 207
Cuts at: 4758 5228 5258 6041 6947 7067 7661 7835 8226
Size: 470 30 783 906 120 594 174 391
Cuts at: 8226 8253 9598 126
Size: 27 1345 604
Fragments arranged by size:
1649 1345 1254 906 783 624 604 594
470 391 364 246 228 207 174 120
60 30 27
EagI C'GGCC_G
Cuts at: 1224 4127 4551 7661 1224
Size: 2903 424 3110 3639
Fragments arranged by size:
3639 3110 2903 424
EarI CTCTTCn'nnn_
Cuts at: 4420 5528 5870 6286 8099 8309 4420
Size: 1108 342 416 1813 210 6187
Fragments arranged by size:
6187 1813 1108 416 342 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3803 4732 8284 8594 8740 9886 3803
Size: 929 3552 310 146 1146 3993
Fragments arranged by size:
3993 3552 1146 929 310 146
EcoICRI GAG'CTC
Cuts at: 5880 7088 5880
Size: 1208 8868
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 1563 1587 2298 3280 4879 5000 5029 5536
Size: 1202 24 711 982 1599 121 29 507
Cuts at: 5536 5552 6227 6587 6632 7901 8333 9080 9348
Size: 16 675 360 45 1269 432 747 268
Cuts at: 9348 361
Size: 1089
Fragments arranged by size:
1599 1269 1202 1089 982 747 711 675
507 432 360 268 121 45 29 24
16
EcoNI CCTnn'n_nnAGG
Cuts at: 7009 7009
Size: 10076
EcoO109I rG'GnC_Cy
Cuts at: 119 345 361 448 517 1420 1421 2059 2060
Size: 226 16 87 69 903 1 638 1
Cuts at: 2060 2877 2918 3164 3398 3625 3626 3780 3826
Size: 817 41 246 234 227 1 154 46
Cuts at: 3826 3904 4069 6194 6226 6248 6302 6334 6335
Size: 78 165 2125 32 22 54 32 1
Cuts at: 6335 6376 6446 6481 6482 6652 6682 6704 7030
Size: 41 70 35 1 170 30 22 326
Cuts at: 7030 7031 7215 119
Size: 1 184 2980
Fragments arranged by size:
2980 2125 903 817 638 326 246 234
227 226 184 170 165 154 87 78
70 69 54 46 41 41 35 32
32 30 22 22 16 1 1 1
1 1 1
EcoRI G'AATT_C
Cuts at: 7080 7080
Size: 10076
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6825 8095 6825
Size: 1270 8806
FspI TGC'GCA
Cuts at: 4726 7857 4726
Size: 3131 6945
HaeII r_GCGC'y
Cuts at: 187 1304 1667 3044 4231 4308 4399 4413 4694
Size: 1117 363 1377 1187 77 91 14 281
Cuts at: 4694 4718 4928 4965 5060 6558 6679 7510 7758
Size: 24 210 37 95 1498 121 831 248
Cuts at: 7758 8780 9759 187
Size: 1022 979 504
Fragments arranged by size:
1498 1377 1187 1117 1022 979 831 504
363 281 248 210 121 95 91 77
37 24 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 518 550 1026 1058 1276 1308 1399 1431 1738
Size: 32 476 32 218 32 91 32 307
Cuts at: 1738 1770 5152 5184 5539 5571 6563 6595 7918
Size: 32 3382 32 355 32 992 32 1323
Cuts at: 7918 7948 7950 7980 518
Size: 30 2 30 2614
Fragments arranged by size:
3382 2614 1323 992 476 355 307 218
91 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 873 1404 1545 7115 873
Size: 531 141 5570 3834
Fragments arranged by size:
5570 3834 531 141
HindIII A'AGCT_T
Cuts at: 3216 3216
Size: 10076
KasI G'GCGC_C
Cuts at: 1300 1663 4227 4409 6554 6675 7506 7754 1300
Size: 363 2564 182 2145 121 831 248 3622
Fragments arranged by size:
3622 2564 2145 831 363 248 182 121
KpnI G_GTAC'C
Cuts at: 977 7096 977
Size: 6119 3957
MluI A'CGCG_T
Cuts at: 9509 9509
Size: 10076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5597 6062 8747 8931 5597
Size: 465 2685 184 6742
Fragments arranged by size:
6742 2685 465 184
MscI TGG'CCA
Cuts at: 998 2480 5230 5260 6949 7837 998
Size: 1482 2750 30 1689 888 3237
Fragments arranged by size:
3237 2750 1689 1482 888 30
MslI CAynn'nnrTG
Cuts at: 426 1069 1160 1193 1948 2201 3652 4487 5389
Size: 643 91 33 755 253 1451 835 902
Cuts at: 5389 5897 6508 8065 8192 426
Size: 508 611 1557 127 2310
Fragments arranged by size:
2310 1557 1451 902 835 755 643 611
508 253 127 91 33
MspA1I CmG'CkG
Cuts at: 279 339 506 569 653 667 700 945 1004
Size: 60 167 63 84 14 33 245 59
Cuts at: 1004 1583 1607 1623 1703 1928 2147 2258 2291
Size: 579 24 16 80 225 219 111 33
Cuts at: 2291 2462 2484 2609 2948 3062 3267 3615 3677
Size: 171 22 125 339 114 205 348 62
Cuts at: 3677 4034 4121 4187 4237 4263 4430 4556 4637
Size: 357 87 66 50 26 167 126 81
Cuts at: 4637 4707 4930 4954 5007 5034 5743 6132 6432
Size: 70 223 24 53 27 709 389 300
Cuts at: 6432 6493 6690 7026 7187 7501 7861 8874 9119
Size: 61 197 336 161 314 360 1013 245
Cuts at: 9119 279
Size: 1236
Fragments arranged by size:
1236 1013 709 579 389 360 357 348
339 336 314 300 245 245 225 223
219 205 197 171 167 167 161 126
125 114 111 87 84 81 80 70
66 63 62 61 60 59 53 50
33 33 27 26 24 24 22 16
14
NaeI GCC'GGC
Cuts at: 2748 4622 5185 8257 2748
Size: 1874 563 3072 4567
Fragments arranged by size:
4567 3072 1874 563
NarI GG'CG_CC
Cuts at: 1301 1664 4228 4410 6555 6676 7507 7755 1301
Size: 363 2564 182 2145 121 831 248 3622
Fragments arranged by size:
3622 2564 2145 831 363 248 182 121
NcoI C'CATG_G
Cuts at: 107 615 1080 1130 1773 1884 2619 4766 5402
Size: 508 465 50 643 111 735 2147 636
Cuts at: 5402 6944 107
Size: 1542 3239
Fragments arranged by size:
3239 2147 1542 735 643 636 508 465
111 50
NgoMIV G'CCGG_C
Cuts at: 2746 4620 5183 8255 2746
Size: 1874 563 3072 4567
Fragments arranged by size:
4567 3072 1874 563
NheI G'CTAG_C
Cuts at: 204 5560 204
Size: 5356 4720
NotI GC'GGCC_GC
Cuts at: 4127 4127
Size: 10076
NsiI A_TGCA'T
Cuts at: 3520 3520
Size: 10076
NspI r_CATG'y
Cuts at: 860 1271 2354 3429 6009 7129 7629 8536 860
Size: 411 1083 1075 2580 1120 500 907 2400
Fragments arranged by size:
2580 2400 1120 1083 1075 907 500 411
PasI CC'CwG_GG
Cuts at: 2505 4057 5760 6700 2505
Size: 1552 1703 940 5881
Fragments arranged by size:
5881 1703 1552 940
PciI A'CATG_T
Cuts at: 3425 8532 3425
Size: 5107 4969
PflMI CCAn_nnn'nTGG
Cuts at: 1281 2304 3105 4767 5267 6222 6757 1281
Size: 1023 801 1662 500 955 535 4600
Fragments arranged by size:
4600 1662 1023 955 801 535 500
PfoI T'CCnGG_A
Cuts at: 828 828
Size: 10076
PmeI GTTT'AAAC
Cuts at: 7075 7075
Size: 10076
PmlI CAC'GTG
Cuts at: 1442 1616 2820 1442
Size: 174 1204 8698
Fragments arranged by size:
8698 1204 174
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2677 2709 9215 9247 2677
Size: 32 6506 32 3506
Fragments arranged by size:
6506 3506 32 32
PpuMI rG'GwC_Cy
Cuts at: 345 2877 2918 3398 3780 3826 3904 4069 6682
Size: 2532 41 480 382 46 78 165 2613
Cuts at: 6682 6704 345
Size: 22 3717
Fragments arranged by size:
3717 2613 2532 480 382 165 78 46
41 22
PshAI GACnn'nnGTC
Cuts at: 350 824 1399 1523 6709 350
Size: 474 575 124 5186 3717
Fragments arranged by size:
5186 3717 575 474 124
PspOMI G'GGCC_C
Cuts at: 119 517 1420 2059 3087 3625 4811 6193 6334
Size: 398 903 639 1028 538 1186 1382 141
Cuts at: 6334 6375 6445 6481 6623 7030 119
Size: 41 70 36 142 407 3165
Fragments arranged by size:
3165 1382 1186 1028 903 639 538 407
398 142 141 70 41 36
PstI C_TGCA'G
Cuts at: 225 745 950 1042 1657 2221 2296 2893 3040
Size: 520 205 92 615 564 75 597 147
Cuts at: 3040 3229 3779 3998 4403 4447 4706 4886 6510
Size: 189 550 219 405 44 259 180 1624
Cuts at: 6510 7123 7808 225
Size: 613 685 2493
Fragments arranged by size:
2493 1624 685 615 613 597 564 550
520 405 259 219 205 189 180 147
92 75 44
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10076
PvuII CAG'CTG
Cuts at: 279 339 569 667 945 1004 1583 1623 2484
Size: 60 230 98 278 59 579 40 861
Cuts at: 2484 2609 2948 3267 4121 5743 6493 6690 7026
Size: 125 339 319 854 1622 750 197 336
Cuts at: 7026 7501 7861 279
Size: 475 360 2494
Fragments arranged by size:
2494 1622 861 854 750 579 475 360
339 336 319 278 230 197 125 98
60 59 40
RsrII CG'GwC_CG
Cuts at: 8271 8271
Size: 10076
SacI G_AGCT'C
Cuts at: 5882 7090 5882
Size: 1208 8868
SacII CC_GC'GG
Cuts at: 701 4035 4238 4264 4638 6133 6433 701
Size: 3334 203 26 374 1495 300 4344
Fragments arranged by size:
4344 3334 1495 374 300 203 26
SalI G'TCGA_C
Cuts at: 7113 7113
Size: 10076
SanDI GG'GwC_CC
Cuts at: 4069 6682 6704 4069
Size: 2613 22 7441
Fragments arranged by size:
7441 2613 22
SapI GCTCTTCn'nnn_
Cuts at: 5870 6286 8099 8309 5870
Size: 416 1813 210 7637
Fragments arranged by size:
7637 1813 416 210
SbfI CC_TGCA'GG
Cuts at: 745 1657 3229 3779 7123 745
Size: 912 1572 550 3344 3698
Fragments arranged by size:
3698 3344 1572 912 550
ScaI AGT'ACT
Cuts at: 4528 9593 4528
Size: 5065 5011
SexAI A'CCwGG_T
Cuts at: 1904 3659 1904
Size: 1755 8321
SfcI C'TryA_G
Cuts at: 33 221 741 946 1038 1653 2217 2292 2889
Size: 188 520 205 92 615 564 75 597
Cuts at: 2889 3036 3225 3775 3994 4399 4443 4702 4882
Size: 147 189 550 219 405 44 259 180
Cuts at: 4882 5795 6506 7119 7804 8797 8988 33
Size: 913 711 613 685 993 191 1121
Fragments arranged by size:
1121 993 913 711 685 615 613 597
564 550 520 405 259 219 205 191
189 188 180 147 92 75 44
SfiI GGCCn_nnn'nGGCC
Cuts at: 4630 6333 4630
Size: 1703 8373
SfoI GGC'GCC
Cuts at: 1302 1665 4229 4411 6556 6677 7508 7756 1302
Size: 363 2564 182 2145 121 831 248 3622
Fragments arranged by size:
3622 2564 2145 831 363 248 182 121
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10076
SgrAI Cr'CCGG_yG
Cuts at: 4486 4486
Size: 10076
SmaI CCC'GGG
Cuts at: 289 2801 3766 3902 4091 4810 6309 6340 6374
Size: 2512 965 136 189 719 1499 31 34
Cuts at: 6374 7098 289
Size: 724 3267
Fragments arranged by size:
3267 2512 1499 965 724 719 189 136
34 31
SmlI C'TyrA_G
Cuts at: 3073 6475 7231 8146 8638 8900 3073
Size: 3402 756 915 492 262 4249
Fragments arranged by size:
4249 3402 915 756 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10076
SphI G_CATG'C
Cuts at: 7129 7629 7129
Size: 500 9576
SrfI GCCC'GGGC
Cuts at: 2801 6374 2801
Size: 3573 6503
StyI C'CwwG_G
Cuts at: 107 615 1080 1130 1773 1884 2619 4766 5231
Size: 508 465 50 643 111 735 2147 465
Cuts at: 5231 5402 6944 7210 7564 107
Size: 171 1542 266 354 2619
Fragments arranged by size:
2619 2147 1542 735 643 508 465 465
354 266 171 111 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 289 309 313 589 982 1411 1580 3655 5077
Size: 20 4 276 393 429 169 2075 1422
Cuts at: 5077 6384 7659 7792 8251 8460 289
Size: 1307 1275 133 459 209 1905
Fragments arranged by size:
2075 1905 1422 1307 1275 459 429 393
276 209 169 133 20 4
TatI w'GTAC_w
Cuts at: 4526 6576 9591 4526
Size: 2050 3015 5011
Fragments arranged by size:
5011 3015 2050
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1230 1262 1932 1964 2168 2200 2809 2841 3253
Size: 32 670 32 204 32 609 32 412
Cuts at: 3253 3285 3390 3422 3982 4014 4135 4167 4249
Size: 32 105 32 560 32 121 32 82
Cuts at: 4249 4281 5266 5298 9593 9625 1230
Size: 32 985 32 4295 32 1681
Fragments arranged by size:
4295 1681 985 670 609 560 412 204
121 105 82 32 32 32 32 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1567 2035 7873 1567
Size: 468 5838 3770
Fragments arranged by size:
5838 3770 468
XbaI T'CTAG_A
Cuts at: 7107 7107
Size: 10076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 664 1366 1620 4337 5268 5679 6078 6942 7571
Size: 702 254 2717 931 411 399 864 629
Cuts at: 7571 664
Size: 3169
Fragments arranged by size:
3169 2717 931 864 702 629 411 399
254
XhoI C'TCGA_G
Cuts at: 3073 3073
Size: 10076
XmaI C'CCGG_G
Cuts at: 287 2799 3764 3900 4089 4808 6307 6338 6372
Size: 2512 965 136 189 719 1499 31 34
Cuts at: 6372 7096 287
Size: 724 3267
Fragments arranged by size:
3267 2512 1499 965 724 719 189 136
34 31
XmnI GAAnn'nnTTC
Cuts at: 2941 2941
Size: 10076
ZraI GAC'GTC
Cuts at: 2407 2407
Size: 10076
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AscI AvrII BsiWI BsrGI BstEII BstZ17I
EcoRV FseI FspAI HpaI MfeI NdeI NruI PacI
PsiI PspXI PsrI SnaBI SspI StuI SwaI