Restriction map for
pF1KB4448
(Circular) (Six-base) MAPSORT of: pF1KB4448.seq Check: 1684 from: 1 to: 3632
pF1KB4448 3632 bp
With 182 enzymes: SgfI *
May 26, 2015 10:13 ..
AccI GT'mk_AC
Cuts at: 670 670
Size: 3632
Acc65I G'GTAC_C
Cuts at: 400 648 400
Size: 248 3384
AclI AA'CG_TT
Cuts at: 3472 3472
Size: 3632
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 1457 1889 2636 2904 292
Size: 1165 432 747 268 1020
Fragments arranged by size:
1165 1020 747 432 268
AflIII A'CryG_T
Cuts at: 984 2088 3065 984
Size: 1104 977 1551
Fragments arranged by size:
1551 1104 977
AgeI A'CCGG_T
Cuts at: 2020 2020
Size: 3632
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 523 555 523
Size: 32 3600
AlwNI CAG_nnn'CTG
Cuts at: 937 2504 937
Size: 1567 2065
ApaI G_GGCC'C
Cuts at: 156 353 156
Size: 197 3435
ApaLI G'TGCA_C
Cuts at: 2402 2402
Size: 3632
ApoI r'AATT_y
Cuts at: 636 2985 3241 636
Size: 2349 256 1027
Fragments arranged by size:
2349 1027 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3632
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3632
AvaI C'yCGr_G
Cuts at: 345 652 345
Size: 307 3325
BamHI G'GATC_C
Cuts at: 657 657
Size: 3632
BanI G'GyrC_C
Cuts at: 142 400 648 1062 1310 1345 142
Size: 258 248 414 248 35 2429
Fragments arranged by size:
2429 414 258 248 248 35
BanII G_rGCy'C
Cuts at: 156 353 646 1676 156
Size: 197 293 1030 2112
Fragments arranged by size:
2112 1030 293 197
BbeI G_GCGC'C
Cuts at: 1066 1314 1066
Size: 248 3384
BbsI GAAGACnn'nnnn_
Cuts at: 127 168 127
Size: 41 3591
BciVI GTATCCnnnnn_n'
Cuts at: 1530 2291 3632 1530
Size: 761 1341 1530
Fragments arranged by size:
1530 1341 761
BclI T'GATC_A
Cuts at: 599 1152 599
Size: 553 3079
BglI GCCn_nnn'nGGC
Cuts at: 151 151
Size: 3632
BglII A'GATC_T
Cuts at: 1147 3184 1147
Size: 2037 1595
BlpI GC'TnA_GC
Cuts at: 394 589 744 394
Size: 195 155 3282
Fragments arranged by size:
3282 195 155
Bme1580I G_kGCm'C
Cuts at: 147 156 353 1257 1350 2406 147
Size: 9 197 904 93 1056 1373
Fragments arranged by size:
1373 1056 904 197 93 9
BmrI ACTGGGnnnn_n'
Cuts at: 844 925 1260 3404 844
Size: 81 335 2144 1072
Fragments arranged by size:
2144 1072 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1999 1999
Size: 3632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1687 2179 2477 808
Size: 879 492 298 1963
Fragments arranged by size:
1963 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 409 3336 409
Size: 2927 705
BsaAI yAC'GTr
Cuts at: 597 985 597
Size: 388 3244
BsaBI GATnn'nnATC
Cuts at: 6 1146 3183 6
Size: 1140 2037 455
Fragments arranged by size:
2037 1140 455
BsaHI Gr'CG_yC
Cuts at: 326 407 1063 1311 2013 3509 326
Size: 81 656 248 702 1496 449
Fragments arranged by size:
1496 702 656 449 248 81
BsaWI w'CCGG_w
Cuts at: 821 1342 2020 2294 2441 2852 821
Size: 521 678 274 147 411 1601
Fragments arranged by size:
1601 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 427 628 637 427
Size: 201 9 3422
Fragments arranged by size:
3422 201 9
BseYI C'CCAG_C
Cuts at: 1057 1815 2392 2888 1057
Size: 758 577 496 1801
Fragments arranged by size:
1801 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1174 1220 2428 104
Size: 1070 46 1208 1308
Fragments arranged by size:
1308 1208 1070 46
BsiHKAI G_wGCw'C
Cuts at: 646 1424 1614 2406 646
Size: 778 190 792 1872
Fragments arranged by size:
1872 792 778 190
BsmI GAATG_Cn'
Cuts at: 220 220
Size: 3632
Bsp1286I G_dGCh'C
Cuts at: 147 156 353 646 1257 1350 1424 1614 1676
Size: 9 197 293 611 93 74 190 62
Cuts at: 1676 2406 147
Size: 730 1373
Fragments arranged by size:
1373 730 611 293 197 190 93 74
62 9
BspEI T'CCGG_A
Cuts at: 2852 2852
Size: 3632
BspHI T'CATG_A
Cuts at: 2808 2808
Size: 3632
BspMI ACCTGCnnnn'nnnn_
Cuts at: 682 896 1198 1579 682
Size: 214 302 381 2735
Fragments arranged by size:
2735 381 302 214
BsrBI CCG'CTC
Cuts at: 50 398 1924 1978 3462 3627 50
Size: 348 1526 54 1484 165 55
Fragments arranged by size:
1526 1484 348 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1544 1544
Size: 3632
BsrFI r'CCGG_y
Cuts at: 1630 1811 2020 1630
Size: 181 209 3242
Fragments arranged by size:
3242 209 181
BssSI C'ACGA_G
Cuts at: 1903 2261 1903
Size: 358 3274
BstAPI GCAn_nnn'nTGC
Cuts at: 1009 1009
Size: 3632
BstBI TT'CG_AA
Cuts at: 1993 1993
Size: 3632
BstXI CCAn_nnnn'nTGG
Cuts at: 561 561
Size: 3632
BstYI r'GATC_y
Cuts at: 1 200 657 1124 1147 1482 1728 2740 2826
Size: 199 457 467 23 335 246 1012 86
Cuts at: 2826 2838 3184 1
Size: 12 346 449
Fragments arranged by size:
1012 467 457 449 346 335 246 199
86 23 12
BtgI C'CryG_G
Cuts at: 107 606 107
Size: 499 3133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1582 1763 1926 1582
Size: 181 163 3288
Fragments arranged by size:
3288 181 163
BtsI GCAGTG_nn'
Cuts at: 875 875
Size: 3632
ClaI AT'CG_AT
Cuts at: 557 557
Size: 3632
DraI TTT'AAA
Cuts at: 631 631
Size: 3632
DrdI GACnn_nn'nnGTC
Cuts at: 1338 2196 1338
Size: 858 2774
EaeI y'GGCC_r
Cuts at: 110 1217 1391 1782 1809 3154 110
Size: 1107 174 391 27 1345 588
Fragments arranged by size:
1345 1107 588 391 174 27
EagI C'GGCC_G
Cuts at: 1217 1217
Size: 3632
EarI CTCTTCn'nnn_
Cuts at: 518 1655 1865 518
Size: 1137 210 2285
Fragments arranged by size:
2285 1137 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 535 1840 2150 2296 3442 535
Size: 1305 310 146 1146 725
Fragments arranged by size:
1305 1146 725 310 146
EcoICRI GAG'CTC
Cuts at: 644 644
Size: 3632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 1457 1889 2636 2904 292
Size: 1165 432 747 268 1020
Fragments arranged by size:
1165 1020 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 267 349 771 267
Size: 82 422 3128
Fragments arranged by size:
3128 422 82
EcoRI G'AATT_C
Cuts at: 636 636
Size: 3632
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1651 1651
Size: 3632
FspI TGC'GCA
Cuts at: 1413 1413
Size: 3632
HaeII r_GCGC'y
Cuts at: 589 1066 1314 2336 3315 589
Size: 477 248 1022 979 906
Fragments arranged by size:
1022 979 906 477 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1474 1504 1506 1536 1474
Size: 30 2 30 3570
Fragments arranged by size:
3570 30 30 2
HincII GTy'rAC
Cuts at: 671 671
Size: 3632
KasI G'GCGC_C
Cuts at: 1062 1310 1062
Size: 248 3384
KpnI G_GTAC'C
Cuts at: 404 652 404
Size: 248 3384
MluI A'CGCG_T
Cuts at: 3065 3065
Size: 3632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 206 2303 2487 206
Size: 2097 184 1351
Fragments arranged by size:
2097 1351 184
MscI TGG'CCA
Cuts at: 112 1393 112
Size: 1281 2351
MslI CAynn'nnrTG
Cuts at: 229 559 1621 1748 229
Size: 330 1062 127 2113
Fragments arranged by size:
2113 1062 330 127
MspA1I CmG'CkG
Cuts at: 743 1057 1417 2430 2675 743
Size: 314 360 1013 245 1700
Fragments arranged by size:
1700 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1813 1813
Size: 3632
NarI GG'CG_CC
Cuts at: 1063 1311 1063
Size: 248 3384
NcoI C'CATG_G
Cuts at: 107 107
Size: 3632
NgoMIV G'CCGG_C
Cuts at: 1811 1811
Size: 3632
NspI r_CATG'y
Cuts at: 685 1185 2092 685
Size: 500 907 2225
Fragments arranged by size:
2225 907 500
PasI CC'CwG_GG
Cuts at: 291 291
Size: 3632
PciI A'CATG_T
Cuts at: 2088 2088
Size: 3632
PfoI T'CCnGG_A
Cuts at: 203 203
Size: 3632
PmeI GTTT'AAAC
Cuts at: 631 631
Size: 3632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2771 2803 2771
Size: 32 3600
PpuMI rG'GwC_Cy
Cuts at: 267 267
Size: 3632
PshAI GACnn'nnGTC
Cuts at: 304 304
Size: 3632
PspOMI G'GGCC_C
Cuts at: 152 349 152
Size: 197 3435
PstI C_TGCA'G
Cuts at: 679 1364 679
Size: 685 2947
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3632
PvuII CAG'CTG
Cuts at: 1057 1417 1057
Size: 360 3272
RsrII CG'GwC_CG
Cuts at: 1827 1827
Size: 3632
SacI G_AGCT'C
Cuts at: 646 646
Size: 3632
SalI G'TCGA_C
Cuts at: 669 669
Size: 3632
SapI GCTCTTCn'nnn_
Cuts at: 1655 1865 1655
Size: 210 3422
SbfI CC_TGCA'GG
Cuts at: 679 679
Size: 3632
ScaI AGT'ACT
Cuts at: 3149 3149
Size: 3632
SfcI C'TryA_G
Cuts at: 33 675 1360 2353 2544 33
Size: 642 685 993 191 1121
Fragments arranged by size:
1121 993 685 642 191
SfoI GGC'GCC
Cuts at: 1064 1312 1064
Size: 248 3384
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3632
SmaI CCC'GGG
Cuts at: 654 654
Size: 3632
SmlI C'TyrA_G
Cuts at: 787 1702 2194 2456 787
Size: 915 492 262 1963
Fragments arranged by size:
1963 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3632
SphI G_CATG'C
Cuts at: 685 1185 685
Size: 500 3132
StuI AGG'CCT
Cuts at: 343 343
Size: 3632
StyI C'CwwG_G
Cuts at: 107 766 1120 107
Size: 659 354 2619
Fragments arranged by size:
2619 659 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 619 1215 1348 1807 2016 619
Size: 596 133 459 209 2235
Fragments arranged by size:
2235 596 459 209 133
TatI w'GTAC_w
Cuts at: 476 3147 476
Size: 2671 961
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3600
Tth111I GACn'n_nGTC
Cuts at: 1429 1429
Size: 3632
XbaI T'CTAG_A
Cuts at: 663 663
Size: 3632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1127 1127
Size: 3632
XmaI C'CCGG_G
Cuts at: 652 652
Size: 3632
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BpmI
Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI