Restriction map for
pF1KB4430
(Circular) (Six-base) MAPSORT of: pF1KB4430.seq Check: 5241 from: 1 to: 4814
pF1KB4430 4814 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1350 1350
Size: 4814
AccI GT'mk_AC
Cuts at: 1448 1852 1448
Size: 404 4410
Acc65I G'GTAC_C
Cuts at: 1830 1830
Size: 4814
AclI AA'CG_TT
Cuts at: 4654 4654
Size: 4814
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 893 916 1024 1704 1802 2639 3071 3424 4086
Size: 23 108 680 98 837 432 353 662
Cuts at: 4086 893
Size: 1621
Fragments arranged by size:
1621 837 680 662 432 353 108 98
23
AflIII A'CryG_T
Cuts at: 160 796 1577 2166 3966 4247 160
Size: 636 781 589 1800 281 727
Fragments arranged by size:
1800 781 727 636 589 281
AleI CACnn'nnGTG
Cuts at: 255 853 1553 255
Size: 598 700 3516
Fragments arranged by size:
3516 700 598
AlwNI CAG_nnn'CTG
Cuts at: 696 1029 1616 2119 3557 696
Size: 333 587 503 1438 1953
Fragments arranged by size:
1953 1438 587 503 333
ApaLI G'TGCA_C
Cuts at: 1234 3652 1234
Size: 2418 2396
ApoI r'AATT_y
Cuts at: 262 1410 1604 1818 4167 4423 262
Size: 1148 194 214 2349 256 653
Fragments arranged by size:
2349 1148 653 256 214 194
AseI AT'TA_AT
Cuts at: 20 20
Size: 4814
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
AvaI C'yCGr_G
Cuts at: 1172 1749 1834 1172
Size: 577 85 4152
Fragments arranged by size:
4152 577 85
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1096 1129 1096
Size: 33 4781
BamHI G'GATC_C
Cuts at: 1839 1839
Size: 4814
BanI G'GyrC_C
Cuts at: 1830 2244 2492 2527 1830
Size: 414 248 35 4117
Fragments arranged by size:
4117 414 248 35
BanII G_rGCy'C
Cuts at: 1255 1828 2858 1255
Size: 573 1030 3211
Fragments arranged by size:
3211 1030 573
BbeI G_GCGC'C
Cuts at: 2248 2496 2248
Size: 248 4566
BbsI GAAGACnn'nnnn_
Cuts at: 976 1054 1790 976
Size: 78 736 4000
Fragments arranged by size:
4000 736 78
BbvCI CC'TCA_GC
Cuts at: 875 1037 875
Size: 162 4652
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 736 770 1149 1183 736
Size: 34 379 34 4367
Fragments arranged by size:
4367 379 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2712 3768 4814 2712
Size: 1056 1046 2712
Fragments arranged by size:
2712 1056 1046
BclI T'GATC_A
Cuts at: 1076 1197 2334 1076
Size: 121 1137 3556
Fragments arranged by size:
3556 1137 121
BglII A'GATC_T
Cuts at: 938 2329 4366 938
Size: 1391 2037 1386
Fragments arranged by size:
2037 1391 1386
BlpI GC'TnA_GC
Cuts at: 1926 1926
Size: 4814
Bme1580I G_kGCm'C
Cuts at: 1238 2439 2532 3656 1238
Size: 1201 93 1124 2396
Fragments arranged by size:
2396 1201 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 643 1695 2026 2107 2442 4586 643
Size: 1052 331 81 335 2144 871
Fragments arranged by size:
2144 1052 871 335 331 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 978 3181 978
Size: 2203 2611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1370 1750 1006
Size: 364 380 4070
Fragments arranged by size:
4070 380 364
Bpu10I CC'TnA_GC
Cuts at: 124 875 1037 124
Size: 751 162 3901
Fragments arranged by size:
3901 751 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 1990 2869 3583 3881 1231
Size: 759 879 714 298 2164
Fragments arranged by size:
2164 879 759 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4518 4518
Size: 4814
BsaAI yAC'GTr
Cuts at: 797 2167 797
Size: 1370 3444
BsaBI GATnn'nnATC
Cuts at: 6 1228 2328 4365 6
Size: 1222 1100 2037 455
Fragments arranged by size:
2037 1222 1100 455
BsaHI Gr'CG_yC
Cuts at: 2245 2493 3195 4691 2245
Size: 248 702 1496 2368
Fragments arranged by size:
2368 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2003 2524 3202 3613 3760 4034 2003
Size: 521 678 411 147 274 2783
Fragments arranged by size:
2783 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 775 805 775
Size: 30 4784
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 967 1054 1770 1783 967
Size: 87 716 13 3998
Fragments arranged by size:
3998 716 87 13
BseYI C'CCAG_C
Cuts at: 1136 1155 1609 1667 2239 2997 3662 4070 1136
Size: 19 454 58 572 758 665 408 1880
Fragments arranged by size:
1880 758 665 572 454 408 58 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 257 127
Size: 130 4684
BsiEI CG_ry'CG
Cuts at: 104 2356 2402 3632 104
Size: 2252 46 1230 1286
Fragments arranged by size:
2252 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 752 1238 1255 1469 1550 1828 2606 2796 3656
Size: 486 17 214 81 278 778 190 860
Cuts at: 3656 752
Size: 1910
Fragments arranged by size:
1910 860 778 486 278 214 190 81
17
BsmBI CGTCTCn'nnnn_
Cuts at: 1645 1696 1645
Size: 51 4763
Bsp1286I G_dGCh'C
Cuts at: 752 1238 1255 1469 1550 1828 2439 2532 2606
Size: 486 17 214 81 278 611 93 74
Cuts at: 2606 2796 2858 3656 752
Size: 190 62 798 1910
Fragments arranged by size:
1910 798 611 486 278 214 190 93
81 74 62 17
BspHI T'CATG_A
Cuts at: 3246 3246
Size: 4814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1154 1291 1350 1864 2078 2380 2761 1154
Size: 137 59 514 214 302 381 3207
Fragments arranged by size:
3207 514 381 302 214 137 59
BsrBI CCG'CTC
Cuts at: 50 779 1044 3106 3160 4644 4809 50
Size: 729 265 2062 54 1484 165 55
Fragments arranged by size:
2062 1484 729 265 165 55 54
BsrDI GCAATG_nn'
Cuts at: 543 2726 543
Size: 2183 2631
BsrFI r'CCGG_y
Cuts at: 683 715 2812 2993 683
Size: 32 2097 181 2504
Fragments arranged by size:
2504 2097 181 32
BssSI C'ACGA_G
Cuts at: 1264 3085 3793 1264
Size: 1821 708 2285
Fragments arranged by size:
2285 1821 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2191 2191
Size: 4814
BstBI TT'CG_AA
Cuts at: 3175 3175
Size: 4814
BstXI CCAn_nnnn'nTGG
Cuts at: 765 843 1461 765
Size: 78 618 4118
Fragments arranged by size:
4118 618 78
BstYI r'GATC_y
Cuts at: 1 938 1839 2306 2329 2664 2910 3216 3228
Size: 937 901 467 23 335 246 306 12
Cuts at: 3228 3314 4366 1
Size: 86 1052 449
Fragments arranged by size:
1052 937 901 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1454 107
Size: 1347 3467
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2764 2945 3108 2764
Size: 181 163 4470
Fragments arranged by size:
4470 181 163
BtsI GCAGTG_nn'
Cuts at: 1502 2057 1502
Size: 555 4259
DraI TTT'AAA
Cuts at: 1813 1813
Size: 4814
DrdI GACnn_nn'nnGTC
Cuts at: 217 2520 3864 217
Size: 2303 1344 1167
Fragments arranged by size:
2303 1344 1167
EaeI y'GGCC_r
Cuts at: 136 2399 2573 2964 2991 4336 136
Size: 2263 174 391 27 1345 614
Fragments arranged by size:
2263 1345 614 391 174 27
EagI C'GGCC_G
Cuts at: 2399 2399
Size: 4814
EarI CTCTTCn'nnn_
Cuts at: 2837 3047 2837
Size: 210 4604
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3022 3764 3910 4624 3022
Size: 742 146 714 3212
Fragments arranged by size:
3212 742 714 146
EcoICRI GAG'CTC
Cuts at: 1253 1826 1253
Size: 573 4241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 893 916 1006 1024 1370 1704 1750 1802 2639
Size: 23 90 18 346 334 46 52 837
Cuts at: 2639 3071 3424 4086 893
Size: 432 353 662 1621
Fragments arranged by size:
1621 837 662 432 353 346 334 90
52 46 23 18
EcoO109I rG'GnC_Cy
Cuts at: 129 1143 1242 1953 129
Size: 1014 99 711 2990
Fragments arranged by size:
2990 1014 711 99
EcoRI G'AATT_C
Cuts at: 1818 1818
Size: 4814
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1002 2833 1002
Size: 1831 2983
FspI TGC'GCA
Cuts at: 1150 2595 1150
Size: 1445 3369
HaeII r_GCGC'y
Cuts at: 2248 2496 3726 4497 2248
Size: 248 1230 771 2565
Fragments arranged by size:
2565 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 763 775 795 807 973 1005 1435 1467 1732
Size: 12 20 12 166 32 430 32 265
Cuts at: 1732 1764 2656 2686 2688 2718 763
Size: 32 892 30 2 30 2859
Fragments arranged by size:
2859 892 430 265 166 32 32 32
30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 681 1658 1853 681
Size: 977 195 3642
Fragments arranged by size:
3642 977 195
HpaI GTT'AAC
Cuts at: 681 681
Size: 4814
KasI G'GCGC_C
Cuts at: 2244 2492 2244
Size: 248 4566
KpnI G_GTAC'C
Cuts at: 1834 1834
Size: 4814
MluI A'CGCG_T
Cuts at: 160 4247 160
Size: 4087 727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3573 3757 3573
Size: 184 4630
MscI TGG'CCA
Cuts at: 2575 2575
Size: 4814
MslI CAynn'nnrTG
Cuts at: 255 853 1553 1639 2803 2930 255
Size: 598 700 86 1164 127 2139
Fragments arranged by size:
2139 1164 700 598 127 86
MspA1I CmG'CkG
Cuts at: 141 1136 1925 2239 2599 3383 3628 141
Size: 995 789 314 360 784 245 1327
Fragments arranged by size:
1327 995 789 784 360 314 245
NaeI GCC'GGC
Cuts at: 2995 2995
Size: 4814
NarI GG'CG_CC
Cuts at: 2245 2493 2245
Size: 248 4566
NcoI C'CATG_G
Cuts at: 107 1454 107
Size: 1347 3467
NgoMIV G'CCGG_C
Cuts at: 2993 2993
Size: 4814
NspI r_CATG'y
Cuts at: 1129 1867 2367 3970 1129
Size: 738 500 1603 1973
Fragments arranged by size:
1973 1603 738 500
PciI A'CATG_T
Cuts at: 3966 3966
Size: 4814
PflMI CCAn_nnn'nTGG
Cuts at: 1616 1707 1616
Size: 91 4723
PfoI T'CCnGG_A
Cuts at: 764 1402 764
Size: 638 4176
PmeI GTTT'AAAC
Cuts at: 1813 1813
Size: 4814
PmlI CAC'GTG
Cuts at: 797 797
Size: 4814
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 4782
PpuMI rG'GwC_Cy
Cuts at: 1143 1242 1143
Size: 99 4715
PshAI GACnn'nnGTC
Cuts at: 1444 1444
Size: 4814
PstI C_TGCA'G
Cuts at: 322 1861 2546 322
Size: 1539 685 2590
Fragments arranged by size:
2590 1539 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4814
PvuII CAG'CTG
Cuts at: 1136 2239 2599 1136
Size: 1103 360 3351
Fragments arranged by size:
3351 1103 360
RsrII CG'GwC_CG
Cuts at: 3009 3009
Size: 4814
SacI G_AGCT'C
Cuts at: 1255 1828 1255
Size: 573 4241
SalI G'TCGA_C
Cuts at: 1851 1851
Size: 4814
SapI GCTCTTCn'nnn_
Cuts at: 2837 3047 2837
Size: 210 4604
SbfI CC_TGCA'GG
Cuts at: 1861 1861
Size: 4814
ScaI AGT'ACT
Cuts at: 4331 4331
Size: 4814
SfcI C'TryA_G
Cuts at: 33 318 519 1105 1857 2542 3510 3701 33
Size: 285 201 586 752 685 968 191 1146
Fragments arranged by size:
1146 968 752 685 586 285 201 191
SfoI GGC'GCC
Cuts at: 2246 2494 2246
Size: 248 4566
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
SmaI CCC'GGG
Cuts at: 1174 1836 1174
Size: 662 4152
SmlI C'TyrA_G
Cuts at: 1246 1969 2884 3598 3860 1246
Size: 723 915 714 262 2200
Fragments arranged by size:
2200 915 723 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4814
SphI G_CATG'C
Cuts at: 1867 2367 1867
Size: 500 4314
StyI C'CwwG_G
Cuts at: 107 1094 1454 1535 1948 2302 107
Size: 987 360 81 413 354 2619
Fragments arranged by size:
2619 987 413 360 354 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1456 2397 2530 2989 3198 1456
Size: 941 133 459 209 3072
Fragments arranged by size:
3072 941 459 209 133
TatI w'GTAC_w
Cuts at: 4329 4329
Size: 4814
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 561 593 4331 4363 561
Size: 32 3738 32 1012
Fragments arranged by size:
3738 1012 32 32
Tth111I GACn'n_nGTC
Cuts at: 2611 2611
Size: 4814
XbaI T'CTAG_A
Cuts at: 1845 1845
Size: 4814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1410 2309 1410
Size: 899 3915
XmaI C'CCGG_G
Cuts at: 1172 1834 1172
Size: 662 4152
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BfrBI BglI BmgBI BmtI BsiWI BsmI
BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII MfeI NdeI
NheI NotI NruI NsiI PacI PasI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI