Restriction map for
pF1KB4407
(Circular) (Six-base) MAPSORT of: pF1KB4407.seq Check: 8317 from: 1 to: 8342
pF1KB4407 8342 bp
With 182 enzymes: SgfI *
January 29, 2016 11:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2674 3208 4254 2674
Size: 534 1046 6762
Fragments arranged by size:
6762 1046 534
AatII G_ACGT'C
Cuts at: 3210 3210
Size: 8342
AccI GT'mk_AC
Cuts at: 910 5380 910
Size: 4470 3872
Acc65I G'GTAC_C
Cuts at: 5358 5358
Size: 8342
AclI AA'CG_TT
Cuts at: 8182 8182
Size: 8342
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 962 1074 1306 1358 1913 2327 3725 5015 6167
Size: 112 232 52 555 414 1398 1290 1152
Cuts at: 6167 6599 7346 7614 962
Size: 432 747 268 1690
Fragments arranged by size:
1690 1398 1290 1152 747 555 432 414
268 232 112 52
AfeI AGC'GCT
Cuts at: 5176 5176
Size: 8342
AflII C'TTAA_G
Cuts at: 490 490
Size: 8342
AflIII A'CryG_T
Cuts at: 2971 3874 5694 6798 7775 2971
Size: 903 1820 1104 977 3538
Fragments arranged by size:
3538 1820 1104 977 903
AgeI A'CCGG_T
Cuts at: 6730 6730
Size: 8342
AhdI GACnn_n'nnGTC
Cuts at: 1737 3951 4587 1737
Size: 2214 636 5492
Fragments arranged by size:
5492 2214 636
AleI CACnn'nnGTG
Cuts at: 4114 4114
Size: 8342
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1540 2614 4286 4555 4753 1540
Size: 1074 1672 269 198 5129
Fragments arranged by size:
5129 1672 1074 269 198
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 205 237 4735 4767 205
Size: 32 4498 32 3780
Fragments arranged by size:
4498 3780 32 32
AlwNI CAG_nnn'CTG
Cuts at: 922 2562 2666 3060 4845 5647 7214 922
Size: 1640 104 394 1785 802 1567 2050
Fragments arranged by size:
2050 1785 1640 1567 802 394 104
ApaI G_GGCC'C
Cuts at: 1624 5220 1624
Size: 3596 4746
ApaLI G'TGCA_C
Cuts at: 2720 7112 2720
Size: 4392 3950
ApoI r'AATT_y
Cuts at: 340 687 811 857 1391 1876 2679 2920 3392
Size: 347 124 46 534 485 803 241 472
Cuts at: 3392 5346 7695 7951 340
Size: 1954 2349 256 731
Fragments arranged by size:
2349 1954 803 731 534 485 472 347
256 241 124 46
AseI AT'TA_AT
Cuts at: 20 20
Size: 8342
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8342
AvaI C'yCGr_G
Cuts at: 998 4575 5089 5362 998
Size: 3577 514 273 3978
Fragments arranged by size:
3978 3577 514 273
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3984 4017 3984
Size: 33 8309
BamHI G'GATC_C
Cuts at: 2835 5367 2835
Size: 2532 5810
BanI G'GyrC_C
Cuts at: 3334 5094 5358 5772 6020 6055 3334
Size: 1760 264 414 248 35 5621
Fragments arranged by size:
5621 1760 414 264 248 35
BanII G_rGCy'C
Cuts at: 823 1023 1624 3449 3985 4685 5090 5145 5220
Size: 200 601 1825 536 700 405 55 75
Cuts at: 5220 5356 6386 823
Size: 136 1030 2779
Fragments arranged by size:
2779 1825 1030 700 601 536 405 200
136 75 55
BbeI G_GCGC'C
Cuts at: 5098 5776 6024 5098
Size: 678 248 7416
Fragments arranged by size:
7416 678 248
BbsI GAAGACnn'nnnn_
Cuts at: 2251 4937 2251
Size: 2686 5656
BbvCI CC'TCA_GC
Cuts at: 704 3488 4505 704
Size: 2784 1017 4541
Fragments arranged by size:
4541 2784 1017
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 796 830 796
Size: 34 8308
BciVI GTATCCnnnnn_n'
Cuts at: 5286 6240 7001 8342 5286
Size: 954 761 1341 5286
Fragments arranged by size:
5286 1341 954 761
BclI T'GATC_A
Cuts at: 1784 3222 3561 4832 5862 1784
Size: 1438 339 1271 1030 4264
Fragments arranged by size:
4264 1438 1271 1030 339
BfrBI ATG'CAT
Cuts at: 2143 2143
Size: 8342
BglI GCCn_nnn'nGGC
Cuts at: 112 4105 112
Size: 3993 4349
BglII A'GATC_T
Cuts at: 1591 5857 7894 1591
Size: 4266 2037 2039
Fragments arranged by size:
4266 2039 2037
BlpI GC'TnA_GC
Cuts at: 3053 4663 5454 3053
Size: 1610 791 5941
Fragments arranged by size:
5941 1610 791
Bme1580I G_kGCm'C
Cuts at: 1624 2121 2724 5220 5967 6060 7116 1624
Size: 497 603 2496 747 93 1056 2850
Fragments arranged by size:
2850 2496 1056 747 603 497 93
BmrI ACTGGGnnnn_n'
Cuts at: 1559 2511 5554 5635 5970 8114 1559
Size: 952 3043 81 335 2144 1787
Fragments arranged by size:
3043 2144 1787 952 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4590 5193 6709 4590
Size: 603 1516 6223
Fragments arranged by size:
6223 1516 603
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1037 3626 3981 4154 4354 4450 4648 4669 4750
Size: 2589 355 173 200 96 198 21 81
Cuts at: 4750 4762 4867 5104 5147 5335 1037
Size: 12 105 237 43 188 4044
Fragments arranged by size:
4044 2589 355 237 200 198 188 173
105 96 81 43 21 12
Bpu10I CC'TnA_GC
Cuts at: 704 1850 3118 3411 3488 4505 704
Size: 1146 1268 293 77 1017 4541
Fragments arranged by size:
4541 1268 1146 1017 293 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 296 2189 3937 5518 6397 6889 7187 296
Size: 1893 1748 1581 879 492 298 1451
Fragments arranged by size:
1893 1748 1581 1451 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 297 2171 3479 8046 297
Size: 1874 1308 4567 593
Fragments arranged by size:
4567 1874 1308 593
BsaAI yAC'GTr
Cuts at: 1473 5695 1473
Size: 4222 4120
BsaBI GATnn'nnATC
Cuts at: 6 2840 3842 4648 5856 7893 6
Size: 2834 1002 806 1208 2037 455
Fragments arranged by size:
2834 2037 1208 1002 806 455
BsaHI Gr'CG_yC
Cuts at: 506 1444 1537 3207 3688 4613 5095 5150 5773
Size: 938 93 1670 481 925 482 55 623
Cuts at: 5773 6021 6723 8219 506
Size: 248 702 1496 629
Fragments arranged by size:
1670 1496 938 925 702 629 623 482
481 248 93 55
BsaWI w'CCGG_w
Cuts at: 1245 4695 5531 6052 6730 7004 7151 7562 1245
Size: 3450 836 521 678 274 147 411 2025
Fragments arranged by size:
3450 2025 836 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1227 1257 4360 4390 4735 4765 5120 5150 1227
Size: 30 3103 30 345 30 355 30 4419
Fragments arranged by size:
4419 3103 355 345 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 1152 1685 2845 3220 3694 3776 3779 3975
Size: 1007 533 1160 375 474 82 3 196
Cuts at: 3975 4351 4354 4579 4726 4813 4816 4819 4894
Size: 376 3 225 147 87 3 3 75
Cuts at: 4894 4984 4987 145
Size: 90 3 3500
Fragments arranged by size:
3500 1160 1007 533 474 376 375 225
196 147 90 87 82 75 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 1022 3756 4127 5767 6525 7102 7598 1022
Size: 2734 371 1640 758 577 496 1766
Fragments arranged by size:
2734 1766 1640 758 577 496 371
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1546 2332 2620 2842 3029 3657 4415 1546
Size: 786 288 222 187 628 758 5473
Fragments arranged by size:
5473 786 758 628 288 222 187
BsiEI CG_ry'CG
Cuts at: 104 123 209 4526 4722 5884 5930 7138 104
Size: 19 86 4317 196 1162 46 1208 1308
Fragments arranged by size:
4317 1308 1208 1162 196 86 46 19
BsiHKAI G_wGCw'C
Cuts at: 823 1714 2494 2724 3449 3985 4685 5356 6134
Size: 891 780 230 725 536 700 671 778
Cuts at: 6134 6324 7116 823
Size: 190 792 2049
Fragments arranged by size:
2049 891 792 780 778 725 700 671
536 230 190
BsmI GAATG_Cn'
Cuts at: 365 809 1026 365
Size: 444 217 7681
Fragments arranged by size:
7681 444 217
BsmBI CGTCTCn'nnnn_
Cuts at: 4128 4982 4128
Size: 854 7488
Bsp1286I G_dGCh'C
Cuts at: 823 1023 1624 1714 2121 2494 2724 3449 3985
Size: 200 601 90 407 373 230 725 536
Cuts at: 3985 4685 5090 5145 5220 5356 5967 6060 6134
Size: 700 405 55 75 136 611 93 74
Cuts at: 6134 6324 6386 7116 823
Size: 190 62 730 2049
Fragments arranged by size:
2049 730 725 700 611 601 536 407
405 373 230 200 190 136 93 90
75 74 62 55
BspEI T'CCGG_A
Cuts at: 1245 7562 1245
Size: 6317 2025
BspHI T'CATG_A
Cuts at: 3287 7518 3287
Size: 4231 4111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1872 2674 2945 3208 4154 4254 5066 5392 5606
Size: 802 271 263 946 100 812 326 214
Cuts at: 5606 5908 6289 1872
Size: 302 381 3925
Fragments arranged by size:
3925 946 812 802 381 326 302 271
263 214 100
BsrBI CCG'CTC
Cuts at: 50 135 1764 3921 4344 4884 6634 6688 8172
Size: 85 1629 2157 423 540 1750 54 1484
Cuts at: 8172 8337 50
Size: 165 55
Fragments arranged by size:
2157 1750 1629 1484 540 423 165 85
55 54
BsrDI GCAATG_nn'
Cuts at: 631 2048 6254 631
Size: 1417 4206 2719
Fragments arranged by size:
4206 2719 1417
BsrFI r'CCGG_y
Cuts at: 176 1985 4104 4183 4721 4958 6340 6521 6730
Size: 1809 2119 79 538 237 1382 181 209
Cuts at: 6730 176
Size: 1788
Fragments arranged by size:
2119 1809 1788 1382 538 237 209 181
79
BsrGI T'GTAC_A
Cuts at: 444 444
Size: 8342
BssHII G'CGCG_C
Cuts at: 5071 5071
Size: 8342
BssSI C'ACGA_G
Cuts at: 6613 6971 6613
Size: 358 7984
BstAPI GCAn_nnn'nTGC
Cuts at: 931 3139 4644 5719 931
Size: 2208 1505 1075 3554
Fragments arranged by size:
3554 2208 1505 1075
BstBI TT'CG_AA
Cuts at: 6703 6703
Size: 8342
BstEII G'GTnAC_C
Cuts at: 2466 4041 2466
Size: 1575 6767
BstXI CCAn_nnnn'nTGG
Cuts at: 180 1381 1455 1722 2987 4116 180
Size: 1201 74 267 1265 1129 4406
Fragments arranged by size:
4406 1265 1201 1129 267 74
BstYI r'GATC_y
Cuts at: 1 918 1591 2748 2835 4673 5119 5367 5834
Size: 917 673 1157 87 1838 446 248 467
Cuts at: 5834 5857 6192 6438 7450 7536 7548 7894 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1838 1157 1012 917 673 467 449 446
346 335 248 246 87 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 518 758 980 518
Size: 240 222 7880
Fragments arranged by size:
7880 240 222
BtgI C'CryG_G
Cuts at: 225 2621 3345 4205 4600 225
Size: 2396 724 860 395 3967
Fragments arranged by size:
3967 2396 860 724 395
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 978 6292 6473 6636 978
Size: 5314 181 163 2684
Fragments arranged by size:
5314 2684 181 163
BtsI GCAGTG_nn'
Cuts at: 2783 2811 5585 2783
Size: 28 2774 5540
Fragments arranged by size:
5540 2774 28
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3287 3322 3287
Size: 35 8307
DraI TTT'AAA
Cuts at: 875 2241 5341 875
Size: 1366 3100 3876
Fragments arranged by size:
3876 3100 1366
DraIII CAC_nnn'GTG
Cuts at: 4653 4653
Size: 8342
DrdI GACnn_nn'nnGTC
Cuts at: 6048 6906 6048
Size: 858 7484
EaeI y'GGCC_r
Cuts at: 120 146 1099 3353 3802 4077 4106 4118 4471
Size: 26 953 2254 449 275 29 12 353
Cuts at: 4471 5927 6101 6492 6519 7864 120
Size: 1456 174 391 27 1345 598
Fragments arranged by size:
2254 1456 1345 953 598 449 391 353
275 174 29 27 26 12
EagI C'GGCC_G
Cuts at: 120 5927 120
Size: 5807 2535
EarI CTCTTCn'nnn_
Cuts at: 610 1129 2303 2422 2434 4753 4854 4901 4946
Size: 519 1174 119 12 2319 101 47 45
Cuts at: 4946 6365 6575 610
Size: 1419 210 2377
Fragments arranged by size:
2377 2319 1419 1174 519 210 119 101
47 45 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 153 3683 4142 4224 6550 6860 7006 8152 153
Size: 3530 459 82 2326 310 146 1146 343
Fragments arranged by size:
3530 2326 1146 459 343 310 146 82
EcoICRI GAG'CTC
Cuts at: 821 3447 3983 4683 5354 821
Size: 2626 536 700 671 3809
Fragments arranged by size:
3809 2626 700 671 536
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 962 1037 1074 1306 1358 1913 2327 3626 3725
Size: 75 37 232 52 555 414 1299 99
Cuts at: 3725 3981 4154 4354 4450 4648 4669 4750 4762
Size: 256 173 200 96 198 21 81 12
Cuts at: 4762 4867 5015 5104 5147 5335 6167 6599 7346
Size: 105 148 89 43 188 832 432 747
Cuts at: 7346 7614 962
Size: 268 1690
Fragments arranged by size:
1690 1299 832 747 555 432 414 268
256 232 200 198 188 173 148 105
99 96 89 81 75 52 43 37
21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 668 839 4336 668
Size: 171 3497 4674
Fragments arranged by size:
4674 3497 171
EcoO109I rG'GnC_Cy
Cuts at: 1620 5216 5217 5481 1620
Size: 3596 1 264 4481
Fragments arranged by size:
4481 3596 264 1
EcoRI G'AATT_C
Cuts at: 1391 1876 5346 1391
Size: 485 3470 4387
Fragments arranged by size:
4387 3470 485
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1194 3907 4585 6361 1194
Size: 2713 678 1776 3175
Fragments arranged by size:
3175 2713 1776 678
FspI TGC'GCA
Cuts at: 4262 4390 4729 4915 6123 4262
Size: 128 339 186 1208 6481
Fragments arranged by size:
6481 1208 339 186 128
FspAI rTGC'GCAy
Cuts at: 4390 4390
Size: 8342
HaeII r_GCGC'y
Cuts at: 4709 4845 4959 5098 5178 5776 6024 7046 8025
Size: 136 114 139 80 598 248 1022 979
Cuts at: 8025 4709
Size: 5026
Fragments arranged by size:
5026 1022 979 598 248 139 136 114
80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1351 1383 1723 1755 2003 2035 2502 2534 3771
Size: 32 340 32 248 32 467 32 1237
Cuts at: 3771 3803 3937 3969 4186 4218 4377 4409 4573
Size: 32 134 32 217 32 159 32 164
Cuts at: 4573 4605 6184 6214 6216 6246 1351
Size: 32 1579 30 2 30 3447
Fragments arranged by size:
3447 1579 1237 467 340 248 217 164
159 134 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2061 5381 2061
Size: 3320 5022
HindIII A'AGCT_T
Cuts at: 944 1309 3905 5291 944
Size: 365 2596 1386 3995
Fragments arranged by size:
3995 2596 1386 365
KasI G'GCGC_C
Cuts at: 5094 5772 6020 5094
Size: 678 248 7416
Fragments arranged by size:
7416 678 248
KpnI G_GTAC'C
Cuts at: 5362 5362
Size: 8342
MluI A'CGCG_T
Cuts at: 7775 7775
Size: 8342
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1337 2024 2167 2807 3237 4063 5118 7013 7197
Size: 687 143 640 430 826 1055 1895 184
Cuts at: 7197 1337
Size: 2482
Fragments arranged by size:
2482 1895 1055 826 687 640 430 184
143
MscI TGG'CCA
Cuts at: 1101 3355 3804 6103 1101
Size: 2254 449 2299 3340
Fragments arranged by size:
3340 2299 2254 449
MslI CAynn'nnrTG
Cuts at: 1031 1406 1522 2443 4114 6331 6458 1031
Size: 375 116 921 1671 2217 127 2915
Fragments arranged by size:
2915 2217 1671 921 375 127 116
MspA1I CmG'CkG
Cuts at: 404 2850 3492 4470 5134 5453 5767 6127 7140
Size: 2446 642 978 664 319 314 360 1013
Cuts at: 7140 7385 404
Size: 245 1361
Fragments arranged by size:
2446 1361 1013 978 664 642 360 319
314 245
NaeI GCC'GGC
Cuts at: 4960 6523 4960
Size: 1563 6779
NarI GG'CG_CC
Cuts at: 5095 5773 6021 5095
Size: 678 248 7416
Fragments arranged by size:
7416 678 248
NcoI C'CATG_G
Cuts at: 225 2621 4205 225
Size: 2396 1584 4362
Fragments arranged by size:
4362 2396 1584
NdeI CA'TA_TG
Cuts at: 1965 3621 3827 1965
Size: 1656 206 6480
Fragments arranged by size:
6480 1656 206
NgoMIV G'CCGG_C
Cuts at: 4958 6521 4958
Size: 1563 6779
NsiI A_TGCA'T
Cuts at: 2145 2145
Size: 8342
NspI r_CATG'y
Cuts at: 400 723 1985 2286 4662 5395 5895 6802 400
Size: 323 1262 301 2376 733 500 907 1940
Fragments arranged by size:
2376 1940 1262 907 733 500 323 301
PciI A'CATG_T
Cuts at: 6798 6798
Size: 8342
PflMI CCAn_nnn'nTGG
Cuts at: 2872 3351 2872
Size: 479 7863
PfoI T'CCnGG_A
Cuts at: 1014 3603 4676 4766 1014
Size: 2589 1073 90 4590
Fragments arranged by size:
4590 2589 1073 90
PmeI GTTT'AAAC
Cuts at: 5341 5341
Size: 8342
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4015 4047 4735 4767 7481 7513 4015
Size: 32 688 32 2714 32 4844
Fragments arranged by size:
4844 2714 688 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1565 2183 5153 1565
Size: 618 2970 4754
Fragments arranged by size:
4754 2970 618
PsiI TTA'TAA
Cuts at: 1918 1918
Size: 8342
PspOMI G'GGCC_C
Cuts at: 1620 5216 1620
Size: 3596 4746
PstI C_TGCA'G
Cuts at: 927 1511 2389 2849 3198 3219 4502 5389 6074
Size: 584 878 460 349 21 1283 887 685
Cuts at: 6074 927
Size: 3195
Fragments arranged by size:
3195 1283 887 878 685 584 460 349
21
PvuI CG_AT'CG
Cuts at: 104 4526 104
Size: 4422 3920
PvuII CAG'CTG
Cuts at: 2850 3492 5134 5767 6127 2850
Size: 642 1642 633 360 5065
Fragments arranged by size:
5065 1642 642 633 360
RsrII CG'GwC_CG
Cuts at: 4698 6537 4698
Size: 1839 6503
SacI G_AGCT'C
Cuts at: 823 3449 3985 4685 5356 823
Size: 2626 536 700 671 3809
Fragments arranged by size:
3809 2626 700 671 536
SalI G'TCGA_C
Cuts at: 5379 5379
Size: 8342
SapI GCTCTTCn'nnn_
Cuts at: 2422 2434 4854 4946 6365 6575 2422
Size: 12 2420 92 1419 210 4189
Fragments arranged by size:
4189 2420 1419 210 92 12
SbfI CC_TGCA'GG
Cuts at: 5389 5389
Size: 8342
ScaI AGT'ACT
Cuts at: 300 7859 300
Size: 7559 783
SexAI A'CCwGG_T
Cuts at: 2979 2979
Size: 8342
SfcI C'TryA_G
Cuts at: 33 914 923 1507 1937 2027 2385 2845 3194
Size: 881 9 584 430 90 358 460 349
Cuts at: 3194 3215 4498 5385 6070 7063 7254 33
Size: 21 1283 887 685 993 191 1121
Fragments arranged by size:
1283 1121 993 887 881 685 584 460
430 358 349 191 90 21 9
SfoI GGC'GCC
Cuts at: 5096 5774 6022 5096
Size: 678 248 7416
Fragments arranged by size:
7416 678 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8342
SgrAI Cr'CCGG_yG
Cuts at: 4958 4958
Size: 8342
SmaI CCC'GGG
Cuts at: 1000 5364 1000
Size: 4364 3978
SmlI C'TyrA_G
Cuts at: 311 490 2204 3916 5497 6412 6904 7166 311
Size: 179 1714 1712 1581 915 492 262 1487
Fragments arranged by size:
1714 1712 1581 1487 915 492 262 179
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8342
SphI G_CATG'C
Cuts at: 1985 5395 5895 1985
Size: 3410 500 4432
Fragments arranged by size:
4432 3410 500
SspI AAT'ATT
Cuts at: 885 2109 885
Size: 1224 7118
StuI AGG'CCT
Cuts at: 1607 1607
Size: 8342
StyI C'CwwG_G
Cuts at: 225 669 1196 2621 3496 4205 4616 5476 5830
Size: 444 527 1425 875 709 411 860 354
Cuts at: 5830 225
Size: 2737
Fragments arranged by size:
2737 1425 875 860 709 527 444 411
354
SwaI ATTT'AAAT
Cuts at: 2241 2241
Size: 8342
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 223 1209 2189 2485 3962 4029 4949 5925 6058
Size: 986 980 296 1477 67 920 976 133
Cuts at: 6058 6517 6726 223
Size: 459 209 1839
Fragments arranged by size:
1839 1477 986 980 976 920 459 296
209 133 67
TatI w'GTAC_w
Cuts at: 298 444 2006 4544 7857 298
Size: 146 1562 2538 3313 783
Fragments arranged by size:
3313 2538 1562 783 146
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1227 1259 2804 2836 3152 3184 4157 4189 7859
Size: 32 1545 32 316 32 973 32 3670
Cuts at: 7859 7891 1227
Size: 32 1678
Fragments arranged by size:
3670 1678 1545 973 316 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3248 6139 3248
Size: 2891 5451
XbaI T'CTAG_A
Cuts at: 1294 2295 5373 1294
Size: 1001 3078 4263
Fragments arranged by size:
4263 3078 1001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1176 4623 5837 1176
Size: 3447 1214 3681
Fragments arranged by size:
3681 3447 1214
XmaI C'CCGG_G
Cuts at: 998 5362 998
Size: 4364 3978
XmnI GAAnn'nnTTC
Cuts at: 747 1055 3890 747
Size: 308 2835 5199
Fragments arranged by size:
5199 2835 308
ZraI GAC'GTC
Cuts at: 3208 3208
Size: 8342
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BmgBI BmtI BsiWI BstZ17I ClaI EcoRV
FseI HpaI MfeI NheI NotI NruI PacI PasI
PmlI PpuMI PspXI PsrI SacII SanDI SfiI SnaBI
SrfI XhoI