Restriction map for
pF1KB4406
(Circular) (Six-base) MAPSORT of: pF1KB4406.seq Check: 412 from: 1 to: 9755
pF1KB4406 9755 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3218 5646 5927 3218
Size: 2428 281 7046
Fragments arranged by size:
7046 2428 281
AatII G_ACGT'C
Cuts at: 2540 6734 2540
Size: 4194 5561
AccI GT'mk_AC
Cuts at: 1376 4877 6793 1376
Size: 3501 1916 4338
Fragments arranged by size:
4338 3501 1916
Acc65I G'GTAC_C
Cuts at: 4427 5844 6771 4427
Size: 1417 927 7411
Fragments arranged by size:
7411 1417 927
AclI AA'CG_TT
Cuts at: 9595 9595
Size: 9755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 1450 2821 4774 6388 7580 8012 8365 9027
Size: 183 1371 1953 1614 1192 432 353 662
Cuts at: 9027 1267
Size: 1995
Fragments arranged by size:
1995 1953 1614 1371 1192 662 432 353
183
AfeI AGC'GCT
Cuts at: 354 2965 4576 4705 354
Size: 2611 1611 129 5404
Fragments arranged by size:
5404 2611 1611 129
AflIII A'CryG_T
Cuts at: 515 846 1025 1664 4360 4555 6293 6386 7107
Size: 331 179 639 2696 195 1738 93 721
Cuts at: 7107 8907 9188 515
Size: 1800 281 1082
Fragments arranged by size:
2696 1800 1738 1082 721 639 331 281
195 179 93
AleI CACnn'nnGTG
Cuts at: 1312 3701 4414 1312
Size: 2389 713 6653
Fragments arranged by size:
6653 2389 713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3014 3014
Size: 9755
AlwNI CAG_nnn'CTG
Cuts at: 210 1876 3662 3975 6187 7060 8498 210
Size: 1666 1786 313 2212 873 1438 1467
Fragments arranged by size:
2212 1786 1666 1467 1438 873 313
ApaI G_GGCC'C
Cuts at: 4355 4355
Size: 9755
ApaLI G'TGCA_C
Cuts at: 427 8593 427
Size: 8166 1589
ApoI r'AATT_y
Cuts at: 548 2218 6356 6759 9108 9364 548
Size: 1670 4138 403 2349 256 939
Fragments arranged by size:
4138 2349 1670 939 403 256
AseI AT'TA_AT
Cuts at: 20 1145 1394 20
Size: 1125 249 8381
Fragments arranged by size:
8381 1125 249
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9755
AvaI C'yCGr_G
Cuts at: 367 470 965 2141 2433 6775 367
Size: 103 495 1176 292 4342 3347
Fragments arranged by size:
4342 3347 1176 495 292 103
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6280 6313 6280
Size: 33 9722
BamHI G'GATC_C
Cuts at: 3798 6780 3798
Size: 2982 6773
BanI G'GyrC_C
Cuts at: 4427 5844 6528 6771 7185 7433 7468 4427
Size: 1417 684 243 414 248 35 6714
Fragments arranged by size:
6714 1417 684 414 248 243 35
BanII G_rGCy'C
Cuts at: 186 374 2748 3119 3926 4355 5130 6769 7799
Size: 188 2374 371 807 429 775 1639 1030
Cuts at: 7799 186
Size: 2142
Fragments arranged by size:
2374 2142 1639 1030 807 775 429 371
188
BbeI G_GCGC'C
Cuts at: 7189 7437 7189
Size: 248 9507
BbsI GAAGACnn'nnnn_
Cuts at: 930 1064 1887 3334 4558 4915 930
Size: 134 823 1447 1224 357 5770
Fragments arranged by size:
5770 1447 1224 823 357 134
BbvCI CC'TCA_GC
Cuts at: 2632 2632
Size: 9755
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 438 472 4015 4049 438
Size: 34 3543 34 6144
Fragments arranged by size:
6144 3543 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3511 3762 7653 8709 9755 3511
Size: 251 3891 1056 1046 3511
Fragments arranged by size:
3891 3511 1056 1046 251
BclI T'GATC_A
Cuts at: 2582 6216 7275 2582
Size: 3634 1059 5062
Fragments arranged by size:
5062 3634 1059
BfrBI ATG'CAT
Cuts at: 6481 6481
Size: 9755
BglI GCCn_nnn'nGGC
Cuts at: 310 3293 3845 4604 6416 310
Size: 2983 552 759 1812 3649
Fragments arranged by size:
3649 2983 1812 759 552
BglII A'GATC_T
Cuts at: 6018 6425 6502 7270 9307 6018
Size: 407 77 768 2037 6466
Fragments arranged by size:
6466 2037 768 407 77
BlpI GC'TnA_GC
Cuts at: 1541 3049 6553 6867 1541
Size: 1508 3504 314 4429
Fragments arranged by size:
4429 3504 1508 314
Bme1580I G_kGCm'C
Cuts at: 431 2762 3176 3737 4355 6533 7380 7473 8597
Size: 2331 414 561 618 2178 847 93 1124
Cuts at: 8597 431
Size: 1589
Fragments arranged by size:
2331 2178 1589 1124 847 618 561 414
93
BmgBI CAC'GTC
Cuts at: 4556 5735 4556
Size: 1179 8576
BmrI ACTGGGnnnn_n'
Cuts at: 441 597 654 1116 2658 2799 3039 3465 5133
Size: 156 57 462 1542 141 240 426 1668
Cuts at: 5133 6139 6967 7048 7383 9527 441
Size: 1006 828 81 335 2144 669
Fragments arranged by size:
2144 1668 1542 1006 828 669 462 426
335 240 156 141 81 57
BmtI G_CTAG'C
Cuts at: 1655 1655
Size: 9755
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8122 8122
Size: 9755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 1457 1552 1592 1706 1930 2620 2947 4936
Size: 999 95 40 114 224 690 327 1989
Cuts at: 4936 5062 5328 6627 458
Size: 126 266 1299 3586
Fragments arranged by size:
3586 1989 1299 999 690 327 266 224
126 114 95 40
Bpu10I CC'TnA_GC
Cuts at: 2632 2632
Size: 9755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 3289 3547 5635 6931 7810 8524 8822 1009
Size: 2280 258 2088 1296 879 714 298 1942
Fragments arranged by size:
2280 2088 1942 1296 879 714 298 258
BsaI GGTCTCn'nnnn_
Cuts at: 1095 1730 1807 2789 4912 9459 1095
Size: 635 77 982 2123 4547 1391
Fragments arranged by size:
4547 2123 1391 982 635 77
BsaAI yAC'GTr
Cuts at: 516 4361 4973 7108 516
Size: 3845 612 2135 3163
Fragments arranged by size:
3845 3163 2135 612
BsaBI GATnn'nnATC
Cuts at: 6 3797 7269 9306 6
Size: 3791 3472 2037 455
Fragments arranged by size:
3791 3472 2037 455
BsaHI Gr'CG_yC
Cuts at: 2537 6731 7186 7434 8136 9632 2537
Size: 4194 455 248 702 1496 2660
Fragments arranged by size:
4194 2660 1496 702 455 248
BsaWI w'CCGG_w
Cuts at: 338 1574 2214 2291 6593 6944 7465 8143 8554
Size: 1236 640 77 4302 351 521 678 411
Cuts at: 8554 8701 8975 338
Size: 147 274 1118
Fragments arranged by size:
4302 1236 1118 678 640 521 411 351
274 147 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2312 2339 2342 2369 4651 4681 6625 6655 2312
Size: 27 3 27 2282 30 1944 30 5412
Fragments arranged by size:
5412 2282 1944 30 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 724 1426 1549 1855 2336 3615 5120 5499 6657
Size: 702 123 306 481 1279 1505 379 1158
Cuts at: 6657 724
Size: 3822
Fragments arranged by size:
3822 1505 1279 1158 702 481 379 306
123
BseYI C'CCAG_C
Cuts at: 2040 3746 6462 6648 7180 7938 8603 9011 2040
Size: 1706 2716 186 532 758 665 408 2784
Fragments arranged by size:
2784 2716 1706 758 665 532 408 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 280 1965 2121 4460 5624 6494 196
Size: 84 1685 156 2339 1164 870 3457
Fragments arranged by size:
3457 2339 1685 1164 870 156 84
BsiEI CG_ry'CG
Cuts at: 104 7297 7343 8573 104
Size: 7193 46 1230 1286
Fragments arranged by size:
7193 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 186 431 1277 1891 2504 4697 6769 7547 7737
Size: 245 846 614 613 2193 2072 778 190
Cuts at: 7737 8597 186
Size: 860 1344
Fragments arranged by size:
2193 2072 1344 860 846 778 614 613
245 190
BsmI GAATG_Cn'
Cuts at: 1586 4543 1586
Size: 2957 6798
Bsp1286I G_dGCh'C
Cuts at: 186 374 431 1277 1891 2504 2748 2762 3119
Size: 188 57 846 614 613 244 14 357
Cuts at: 3119 3176 3737 3926 4355 4697 5130 6533 6769
Size: 57 561 189 429 342 433 1403 236
Cuts at: 6769 7380 7473 7547 7737 7799 8597 186
Size: 611 93 74 190 62 798 1344
Fragments arranged by size:
1403 1344 846 798 614 613 611 561
433 429 357 342 244 236 190 189
188 93 74 62 57 57 14
BspEI T'CCGG_A
Cuts at: 338 1574 2214 338
Size: 1236 640 7879
Fragments arranged by size:
7879 1236 640
BspHI T'CATG_A
Cuts at: 833 8187 833
Size: 7354 2401
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3218 4405 5646 5927 6805 7019 7321 7702 3218
Size: 1187 1241 281 878 214 302 381 5271
Fragments arranged by size:
5271 1241 1187 878 381 302 281 214
BsrBI CCG'CTC
Cuts at: 50 336 2022 2940 3623 8047 8101 9585 9750
Size: 286 1686 918 683 4424 54 1484 165
Cuts at: 9750 50
Size: 55
Fragments arranged by size:
4424 1686 1484 918 683 286 165 55
54
BsrDI GCAATG_nn'
Cuts at: 1379 1760 3002 3168 3831 7667 1379
Size: 381 1242 166 663 3836 3467
Fragments arranged by size:
3836 3467 1242 663 381 166
BsrFI r'CCGG_y
Cuts at: 1297 2112 5695 7753 7934 1297
Size: 815 3583 2058 181 3118
Fragments arranged by size:
3583 3118 2058 815 181
BsrGI T'GTAC_A
Cuts at: 1667 4772 6581 1667
Size: 3105 1809 4841
Fragments arranged by size:
4841 3105 1809
BssHII G'CGCG_C
Cuts at: 224 292 224
Size: 68 9687
BssSI C'ACGA_G
Cuts at: 253 2755 8026 8734 253
Size: 2502 5271 708 1274
Fragments arranged by size:
5271 2502 1274 708
BstAPI GCAn_nnn'nTGC
Cuts at: 210 6190 7132 210
Size: 5980 942 2833
Fragments arranged by size:
5980 2833 942
BstBI TT'CG_AA
Cuts at: 4535 8116 4535
Size: 3581 6174
BstEII G'GTnAC_C
Cuts at: 2006 2006
Size: 9755
BstXI CCAn_nnnn'nTGG
Cuts at: 2748 3490 5146 6430 2748
Size: 742 1656 1284 6073
Fragments arranged by size:
6073 1656 1284 742
BstYI r'GATC_y
Cuts at: 1 2211 3798 4059 6018 6425 6502 6780 7247
Size: 2210 1587 261 1959 407 77 278 467
Cuts at: 7247 7270 7605 7851 8157 8169 8255 9307 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
2210 1959 1587 1052 467 449 407 335
306 278 261 246 86 77 23 12
Bsu36I CC'TnA_GG
Cuts at: 3290 5038 3290
Size: 1748 8007
BtgI C'CryG_G
Cuts at: 107 1740 1958 3986 5145 5411 5948 6737 107
Size: 1633 218 2028 1159 266 537 789 3125
Fragments arranged by size:
3125 2028 1633 1159 789 537 266 218
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1143 1605 3443 4530 4754 7705 7886 8049 1143
Size: 462 1838 1087 224 2951 181 163 2849
Fragments arranged by size:
2951 2849 1838 1087 462 224 181 163
BtsI GCAGTG_nn'
Cuts at: 166 510 1065 1308 1686 2483 3258 3735 3915
Size: 344 555 243 378 797 775 477 180
Cuts at: 3915 3976 4245 4395 4484 4761 5684 6998 166
Size: 61 269 150 89 277 923 1314 2923
Fragments arranged by size:
2923 1314 923 797 775 555 477 378
344 277 269 243 180 150 89 61
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3057 3092 3396 3431 4491 4526 4576 4611 3057
Size: 35 304 35 1060 35 50 35 8201
Fragments arranged by size:
8201 1060 304 50 35 35 35 35
DraI TTT'AAA
Cuts at: 6251 6354 6754 6251
Size: 103 400 9252
Fragments arranged by size:
9252 400 103
DraIII CAC_nnn'GTG
Cuts at: 1356 2853 1356
Size: 1497 8258
DrdI GACnn_nn'nnGTC
Cuts at: 395 7461 8805 395
Size: 7066 1344 1345
Fragments arranged by size:
7066 1345 1344
EaeI y'GGCC_r
Cuts at: 819 1098 1757 2955 4242 4584 4605 7340 7514
Size: 279 659 1198 1287 342 21 2735 174
Cuts at: 7514 7905 7932 9277 819
Size: 391 27 1345 1297
Fragments arranged by size:
2735 1345 1297 1287 1198 659 391 342
279 174 27 21
EagI C'GGCC_G
Cuts at: 7340 7340
Size: 9755
EarI CTCTTCn'nnn_
Cuts at: 701 1879 2369 3323 5758 7778 7988 701
Size: 1178 490 954 2435 2020 210 2468
Fragments arranged by size:
2468 2435 2020 1178 954 490 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1760 2082 7963 8705 8851 9565 1760
Size: 322 5881 742 146 714 1950
Fragments arranged by size:
5881 1950 742 714 322 146
EcoICRI GAG'CTC
Cuts at: 184 6767 184
Size: 6583 3172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 1267 1450 1457 1552 1592 1706 1930 2620
Size: 809 183 7 95 40 114 224 690
Cuts at: 2620 2821 2947 4774 4936 5062 5328 6388 6627
Size: 201 126 1827 162 126 266 1060 239
Cuts at: 6627 7580 8012 8365 9027 458
Size: 953 432 353 662 1186
Fragments arranged by size:
1827 1186 1060 953 809 690 662 432
353 266 239 224 201 183 162 126
126 114 95 40 7
EcoNI CCTnn'n_nnAGG
Cuts at: 1314 1594 3202 4833 5208 1314
Size: 280 1608 1631 375 5861
Fragments arranged by size:
5861 1631 1608 375 280
EcoO109I rG'GnC_Cy
Cuts at: 779 3080 3270 4352 4394 5042 6894 779
Size: 2301 190 1082 42 648 1852 3640
Fragments arranged by size:
3640 2301 1852 1082 648 190 42
EcoRI G'AATT_C
Cuts at: 6759 6759
Size: 9755
EcoRV GAT'ATC
Cuts at: 5364 5424 5364
Size: 60 9695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 582 1003 5598 6263 7774 582
Size: 421 4595 665 1511 2563
Fragments arranged by size:
4595 2563 1511 665 421
FspI TGC'GCA
Cuts at: 7536 7536
Size: 9755
HaeII r_GCGC'y
Cuts at: 356 1548 2550 2967 4578 4707 7189 7437 8667
Size: 1192 1002 417 1611 129 2482 248 1230
Cuts at: 8667 9438 356
Size: 771 673
Fragments arranged by size:
2482 1611 1230 1192 1002 771 673 417
248 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1415 1447 1484 1516 2339 2371 2416 2448 2696
Size: 32 37 32 823 32 45 32 248
Cuts at: 2696 2728 3025 3057 3229 3261 4103 4135 5305
Size: 32 297 32 172 32 842 32 1170
Cuts at: 5305 5337 6451 6483 7597 7627 7629 7659 1415
Size: 32 1114 32 1114 30 2 30 3511
Fragments arranged by size:
3511 1170 1114 1114 842 823 297 248
172 45 37 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3807 4766 5253 6794 3807
Size: 959 487 1541 6768
Fragments arranged by size:
6768 1541 959 487
HindIII A'AGCT_T
Cuts at: 2612 5397 2612
Size: 2785 6970
KasI G'GCGC_C
Cuts at: 7185 7433 7185
Size: 248 9507
KpnI G_GTAC'C
Cuts at: 4431 5848 6775 4431
Size: 1417 927 7411
Fragments arranged by size:
7411 1417 927
MfeI C'AATT_G
Cuts at: 1503 4620 6005 1503
Size: 3117 1385 5253
Fragments arranged by size:
5253 3117 1385
MluI A'CGCG_T
Cuts at: 9188 9188
Size: 9755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 779 2150 2546 2727 3737 4872 6074 6150 6386
Size: 1371 396 181 1010 1135 1202 76 236
Cuts at: 6386 8514 8698 779
Size: 2128 184 1836
Fragments arranged by size:
2128 1836 1371 1202 1135 1010 396 236
184 181 76
MscI TGG'CCA
Cuts at: 821 2957 4244 7516 821
Size: 2136 1287 3272 3060
Fragments arranged by size:
3272 3060 2136 1287
MslI CAynn'nnrTG
Cuts at: 379 1312 2887 2996 3124 3701 3875 4414 5144
Size: 933 1575 109 128 577 174 539 730
Cuts at: 5144 5921 6266 7744 7871 379
Size: 777 345 1478 127 2263
Fragments arranged by size:
2263 1575 1478 933 777 730 577 539
345 174 128 127 109
MspA1I CmG'CkG
Cuts at: 727 798 1934 2640 3750 3836 4382 4801 5082
Size: 71 1136 706 1110 86 546 419 281
Cuts at: 5082 6866 7180 7540 8324 8569 727
Size: 1784 314 360 784 245 1913
Fragments arranged by size:
1913 1784 1136 1110 784 706 546 419
360 314 281 245 86 71
NaeI GCC'GGC
Cuts at: 1299 7936 1299
Size: 6637 3118
NarI GG'CG_CC
Cuts at: 7186 7434 7186
Size: 248 9507
NcoI C'CATG_G
Cuts at: 107 3986 5145 5411 6737 107
Size: 3879 1159 266 1326 3125
Fragments arranged by size:
3879 3125 1326 1159 266
NdeI CA'TA_TG
Cuts at: 4903 4903
Size: 9755
NgoMIV G'CCGG_C
Cuts at: 1297 7934 1297
Size: 6637 3118
NheI G'CTAG_C
Cuts at: 1651 1651
Size: 9755
NruI TCG'CGA
Cuts at: 138 138
Size: 9755
NsiI A_TGCA'T
Cuts at: 6483 6483
Size: 9755
NspI r_CATG'y
Cuts at: 850 1029 2087 3615 5614 5926 6297 6808 7308
Size: 179 1058 1528 1999 312 371 511 500
Cuts at: 7308 8911 850
Size: 1603 1694
Fragments arranged by size:
1999 1694 1603 1528 1058 511 500 371
312 179
PasI CC'CwG_GG
Cuts at: 1518 1767 2741 5093 1518
Size: 249 974 2352 6180
Fragments arranged by size:
6180 2352 974 249
PciI A'CATG_T
Cuts at: 846 1025 6293 8907 846
Size: 179 5268 2614 1694
Fragments arranged by size:
5268 2614 1694 179
PflMI CCAn_nnn'nTGG
Cuts at: 1356 1773 3987 4262 5198 1356
Size: 417 2214 275 936 5913
Fragments arranged by size:
5913 2214 936 417 275
PfoI T'CCnGG_A
Cuts at: 1710 4718 5891 1710
Size: 3008 1173 5574
Fragments arranged by size:
5574 3008 1173
PmeI GTTT'AAAC
Cuts at: 6754 6754
Size: 9755
PmlI CAC'GTG
Cuts at: 4361 4361
Size: 9755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2368 2400 8201 8233 2368
Size: 32 5801 32 3890
Fragments arranged by size:
5801 3890 32 32
PpuMI rG'GwC_Cy
Cuts at: 779 3080 3270 4394 5042 779
Size: 2301 190 1124 648 5492
Fragments arranged by size:
5492 2301 1124 648 190
PspOMI G'GGCC_C
Cuts at: 4351 4351
Size: 9755
PstI C_TGCA'G
Cuts at: 365 3229 3364 3604 3658 4387 4756 5679 6605
Size: 2864 135 240 54 729 369 923 926
Cuts at: 6605 6802 7487 365
Size: 197 685 2633
Fragments arranged by size:
2864 2633 926 923 729 685 369 240
197 135 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9755
PvuII CAG'CTG
Cuts at: 727 798 1934 2640 4801 5082 7180 7540 727
Size: 71 1136 706 2161 281 2098 360 2942
Fragments arranged by size:
2942 2161 2098 1136 706 360 281 71
RsrII CG'GwC_CG
Cuts at: 7950 7950
Size: 9755
SacI G_AGCT'C
Cuts at: 186 6769 186
Size: 6583 3172
SalI G'TCGA_C
Cuts at: 6792 6792
Size: 9755
SanDI GG'GwC_CC
Cuts at: 3080 3080
Size: 9755
SapI GCTCTTCn'nnn_
Cuts at: 1879 5758 7778 7988 1879
Size: 3879 2020 210 3646
Fragments arranged by size:
3879 3646 2020 210
SbfI CC_TGCA'GG
Cuts at: 6802 6802
Size: 9755
ScaI AGT'ACT
Cuts at: 9272 9272
Size: 9755
SexAI A'CCwGG_T
Cuts at: 1178 2849 1178
Size: 1671 8084
SfcI C'TryA_G
Cuts at: 33 361 3225 3360 3600 3654 4383 4752 4881
Size: 328 2864 135 240 54 729 369 129
Cuts at: 4881 5675 6601 6636 6798 7483 8451 8642 33
Size: 794 926 35 162 685 968 191 1146
Fragments arranged by size:
2864 1146 968 926 794 729 685 369
328 240 191 162 135 129 54 35
SfoI GGC'GCC
Cuts at: 7187 7435 7187
Size: 248 9507
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9755
SmaI CCC'GGG
Cuts at: 967 2143 6777 967
Size: 1176 4634 3945
Fragments arranged by size:
4634 3945 1176
SmlI C'TyrA_G
Cuts at: 988 3304 3526 5650 6910 7825 8539 8801 988
Size: 2316 222 2124 1260 915 714 262 1942
Fragments arranged by size:
2316 2124 1942 1260 915 714 262 222
SpeI A'CTAG_T
Cuts at: 67 5287 67
Size: 5220 4535
SphI G_CATG'C
Cuts at: 6808 7308 6808
Size: 500 9255
SspI AAT'ATT
Cuts at: 1153 1153
Size: 9755
StuI AGG'CCT
Cuts at: 751 1613 3709 4684 4750 751
Size: 862 2096 975 66 5756
Fragments arranged by size:
5756 2096 975 862 66
StyI C'CwwG_G
Cuts at: 107 464 1262 1310 1595 1967 2531 3203 3986
Size: 357 798 48 285 372 564 672 783
Cuts at: 3986 5145 5411 6327 6737 6889 7243 107
Size: 1159 266 916 410 152 354 2619
Fragments arranged by size:
2619 1159 916 798 783 672 564 410
372 357 354 285 266 152 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1416 3484 4404 4519 4538 5158 5485 7338 7471
Size: 2068 920 115 19 620 327 1853 133
Cuts at: 7471 7930 8139 1416
Size: 459 209 3032
Fragments arranged by size:
3032 2068 1853 920 620 459 327 209
133 115 19
TatI w'GTAC_w
Cuts at: 946 1022 1667 3593 4772 4779 4986 5162 6581
Size: 76 645 1926 1179 7 207 176 1419
Cuts at: 6581 9270 946
Size: 2689 1431
Fragments arranged by size:
2689 1926 1431 1419 1179 645 207 176
76 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1122 1154 2140 2172 2854 2884 2886 2916 3262
Size: 32 986 32 682 30 2 30 346
Cuts at: 3262 3294 3937 3969 4563 4595 4651 4683 5348
Size: 32 643 32 594 32 56 32 665
Cuts at: 5348 5380 6625 6657 9272 9304 1122
Size: 32 1245 32 2615 32 1573
Fragments arranged by size:
2615 1573 1245 986 682 665 643 594
346 56 32 32 32 32 32 32
32 32 32 30 30 2
Tth111I GACn'n_nGTC
Cuts at: 7552 7552
Size: 9755
XbaI T'CTAG_A
Cuts at: 6786 6786
Size: 9755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 762 1632 1965 3459 4105 5580 6369 7250 762
Size: 870 333 1494 646 1475 789 881 3267
Fragments arranged by size:
3267 1494 1475 881 870 789 646 333
XmaI C'CCGG_G
Cuts at: 965 2141 6775 965
Size: 1176 4634 3945
Fragments arranged by size:
4634 3945 1176
XmnI GAAnn'nnTTC
Cuts at: 1825 2383 3133 5452 1825
Size: 558 750 2319 6128
Fragments arranged by size:
6128 2319 750 558
ZraI GAC'GTC
Cuts at: 2538 6732 2538
Size: 4194 5561
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AloI AscI AvrII BsiWI BsmBI
BstZ17I ClaI FseI FspAI HpaI NotI PacI PshAI
PsiI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI
SwaI XhoI