Restriction map for
pF1KB4375
(Circular) (Six-base) MAPSORT of: pF1KB4375.seq Check: 7893 from: 1 to: 4280
pF1KB4375 4280 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 278 278
Size: 4280
AccI GT'mk_AC
Cuts at: 1028 1318 1028
Size: 290 3990
Acc65I G'GTAC_C
Cuts at: 1296 1296
Size: 4280
AclI AA'CG_TT
Cuts at: 4120 4120
Size: 4280
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 569 2105 2537 2890 3552 569
Size: 1536 432 353 662 1297
Fragments arranged by size:
1536 1297 662 432 353
AflIII A'CryG_T
Cuts at: 1632 3432 3713 1632
Size: 1800 281 2199
Fragments arranged by size:
2199 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1170 1170
Size: 4280
AlwNI CAG_nnn'CTG
Cuts at: 1585 3023 1585
Size: 1438 2842
ApaI G_GGCC'C
Cuts at: 198 198
Size: 4280
ApaLI G'TGCA_C
Cuts at: 1095 1195 3118 1095
Size: 100 1923 2257
Fragments arranged by size:
2257 1923 100
ApoI r'AATT_y
Cuts at: 516 710 728 1284 3633 3889 516
Size: 194 18 556 2349 256 907
Fragments arranged by size:
2349 907 556 256 194 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 4280
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
AvaI C'yCGr_G
Cuts at: 203 348 836 1300 203
Size: 145 488 464 3183
Fragments arranged by size:
3183 488 464 145
BamHI G'GATC_C
Cuts at: 1305 1305
Size: 4280
BanI G'GyrC_C
Cuts at: 352 361 370 497 1003 1296 1710 1958 1993
Size: 9 9 127 506 293 414 248 35
Cuts at: 1993 352
Size: 2639
Fragments arranged by size:
2639 506 414 293 248 127 35 9
9
BanII G_rGCy'C
Cuts at: 198 1294 2324 198
Size: 1096 1030 2154
Fragments arranged by size:
2154 1096 1030
BbeI G_GCGC'C
Cuts at: 1714 1962 1714
Size: 248 4032
BciVI GTATCCnnnnn_n'
Cuts at: 2178 3234 4280 2178
Size: 1056 1046 2178
Fragments arranged by size:
2178 1056 1046
BclI T'GATC_A
Cuts at: 1800 1800
Size: 4280
BglI GCCn_nnn'nGGC
Cuts at: 344 389 344
Size: 45 4235
BglII A'GATC_T
Cuts at: 1795 3832 1795
Size: 2037 2243
BlpI GC'TnA_GC
Cuts at: 1392 1392
Size: 4280
Bme1580I G_kGCm'C
Cuts at: 198 355 500 1099 1199 1905 1998 3122 198
Size: 157 145 599 100 706 93 1124 1356
Fragments arranged by size:
1356 1124 706 599 157 145 100 93
BmrI ACTGGGnnnn_n'
Cuts at: 607 1492 1573 1908 4052 607
Size: 885 81 335 2144 835
Fragments arranged by size:
2144 885 835 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 318 2647 318
Size: 2329 1951
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 671 749 784 1006 653
Size: 18 78 35 222 3927
Fragments arranged by size:
3927 222 78 35 18
Bpu10I CC'TnA_GC
Cuts at: 376 440 376
Size: 64 4216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 767 1147 1456 2335 3049 3347 767
Size: 380 309 879 714 298 1700
Fragments arranged by size:
1700 879 714 380 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 935 3984 935
Size: 3049 1231
BsaAI yAC'GTr
Cuts at: 1633 1633
Size: 4280
BsaBI GATnn'nnATC
Cuts at: 6 1794 3831 6
Size: 1788 2037 455
Fragments arranged by size:
2037 1788 455
BsaHI Gr'CG_yC
Cuts at: 1151 1711 1959 2661 4157 1151
Size: 560 248 702 1496 1274
Fragments arranged by size:
1496 1274 702 560 248
BsaWI w'CCGG_w
Cuts at: 1469 1990 2668 3079 3226 3500 1469
Size: 521 678 411 147 274 2249
Fragments arranged by size:
2249 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 469 499 469
Size: 30 4250
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1150 1150
Size: 4280
BseYI C'CCAG_C
Cuts at: 1254 1705 2463 3128 3536 1254
Size: 451 758 665 408 1998
Fragments arranged by size:
1998 758 665 451 408
BsiEI CG_ry'CG
Cuts at: 104 1822 1868 3098 104
Size: 1718 46 1230 1286
Fragments arranged by size:
1718 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 920 1099 1199 1294 2072 2262 3122 920
Size: 179 100 95 778 190 860 2078
Fragments arranged by size:
2078 860 778 190 179 100 95
BsmBI CGTCTCn'nnnn_
Cuts at: 1105 1105
Size: 4280
Bsp1286I G_dGCh'C
Cuts at: 198 355 500 920 1099 1199 1294 1905 1998
Size: 157 145 420 179 100 95 611 93
Cuts at: 1998 2072 2262 2324 3122 198
Size: 74 190 62 798 1356
Fragments arranged by size:
1356 798 611 420 190 179 157 145
100 95 93 74 62
BspHI T'CATG_A
Cuts at: 2712 2712
Size: 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 1330 1544 1846 2227 278
Size: 1052 214 302 381 2331
Fragments arranged by size:
2331 1052 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2572 2626 4110 4275 50
Size: 2522 54 1484 165 55
Fragments arranged by size:
2522 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 600 2192 600
Size: 1592 2688
BsrFI r'CCGG_y
Cuts at: 2278 2459 2278
Size: 181 4099
BssSI C'ACGA_G
Cuts at: 2551 3259 2551
Size: 708 3572
BstAPI GCAn_nnn'nTGC
Cuts at: 1657 1657
Size: 4280
BstBI TT'CG_AA
Cuts at: 2641 2641
Size: 4280
BstXI CCAn_nnnn'nTGG
Cuts at: 1254 1254
Size: 4280
BstYI r'GATC_y
Cuts at: 1 1305 1772 1795 2130 2376 2682 2694 2780
Size: 1304 467 23 335 246 306 12 86
Cuts at: 2780 3832 1
Size: 1052 449
Fragments arranged by size:
1304 1052 467 449 335 306 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1029 1029
Size: 4280
BtgI C'CryG_G
Cuts at: 107 157 107
Size: 50 4230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2230 2411 2574 2230
Size: 181 163 3936
Fragments arranged by size:
3936 181 163
BtsI GCAGTG_nn'
Cuts at: 1099 1523 1099
Size: 424 3856
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1221 1256 1221
Size: 35 4245
DraI TTT'AAA
Cuts at: 1279 1279
Size: 4280
DrdI GACnn_nn'nnGTC
Cuts at: 758 1986 3330 758
Size: 1228 1344 1708
Fragments arranged by size:
1708 1344 1228
EaeI y'GGCC_r
Cuts at: 1865 2039 2430 2457 3802 1865
Size: 174 391 27 1345 2343
Fragments arranged by size:
2343 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1865 1865
Size: 4280
EarI CTCTTCn'nnn_
Cuts at: 238 2303 2513 238
Size: 2065 210 2005
Fragments arranged by size:
2065 2005 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2488 3230 3376 4090 2488
Size: 742 146 714 2678
Fragments arranged by size:
2678 742 714 146
EcoICRI GAG'CTC
Cuts at: 1292 1292
Size: 4280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 569 653 671 749 784 1006 2105 2537 2890
Size: 84 18 78 35 222 1099 432 353
Cuts at: 2890 3552 569
Size: 662 1297
Fragments arranged by size:
1297 1099 662 432 353 222 84 78
35 18
EcoNI CCTnn'n_nnAGG
Cuts at: 1162 1162
Size: 4280
EcoO109I rG'GnC_Cy
Cuts at: 194 195 646 673 682 691 700 769 787
Size: 1 451 27 9 9 9 69 18
Cuts at: 787 805 1419 194
Size: 18 614 3055
Fragments arranged by size:
3055 614 451 69 27 18 18 9
9 9 1
EcoRI G'AATT_C
Cuts at: 710 728 1284 710
Size: 18 556 3706
Fragments arranged by size:
3706 556 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 4280
FspI TGC'GCA
Cuts at: 2061 2061
Size: 4280
HaeII r_GCGC'y
Cuts at: 157 1714 1962 3192 3963 157
Size: 1557 248 1230 771 474
Fragments arranged by size:
1557 1230 771 474 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2122 2152 2154 2184 2122
Size: 30 2 30 4218
Fragments arranged by size:
4218 30 30 2
HincII GTy'rAC
Cuts at: 1319 1319
Size: 4280
KasI G'GCGC_C
Cuts at: 1710 1958 1710
Size: 248 4032
KpnI G_GTAC'C
Cuts at: 1300 1300
Size: 4280
MluI A'CGCG_T
Cuts at: 3713 3713
Size: 4280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3039 3223 3039
Size: 184 4096
MscI TGG'CCA
Cuts at: 2041 2041
Size: 4280
MslI CAynn'nnrTG
Cuts at: 940 1216 2269 2396 940
Size: 276 1053 127 2824
Fragments arranged by size:
2824 1053 276 127
MspA1I CmG'CkG
Cuts at: 159 224 853 977 1391 1705 2065 2849 3094
Size: 65 629 124 414 314 360 784 245
Cuts at: 3094 159
Size: 1345
Fragments arranged by size:
1345 784 629 414 360 314 245 124
65
NaeI GCC'GGC
Cuts at: 2461 2461
Size: 4280
NarI GG'CG_CC
Cuts at: 1711 1959 1711
Size: 248 4032
NcoI C'CATG_G
Cuts at: 107 107
Size: 4280
NgoMIV G'CCGG_C
Cuts at: 2459 2459
Size: 4280
NspI r_CATG'y
Cuts at: 1333 1833 3436 1333
Size: 500 1603 2177
Fragments arranged by size:
2177 1603 500
PasI CC'CwG_GG
Cuts at: 696 696
Size: 4280
PciI A'CATG_T
Cuts at: 3432 3432
Size: 4280
PfoI T'CCnGG_A
Cuts at: 651 669 687 723 732 651
Size: 18 18 36 9 4199
Fragments arranged by size:
4199 36 18 18 9
PmeI GTTT'AAAC
Cuts at: 1279 1279
Size: 4280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2726 2758 2726
Size: 32 4248
PpuMI rG'GwC_Cy
Cuts at: 646 673 682 691 700 769 787 805 646
Size: 27 9 9 9 69 18 18 4121
Fragments arranged by size:
4121 69 27 18 18 9 9 9
PspOMI G'GGCC_C
Cuts at: 194 194
Size: 4280
PstI C_TGCA'G
Cuts at: 150 1094 1327 2012 150
Size: 944 233 685 2418
Fragments arranged by size:
2418 944 685 233
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4280
PvuII CAG'CTG
Cuts at: 224 853 1705 2065 224
Size: 629 852 360 2439
Fragments arranged by size:
2439 852 629 360
RsrII CG'GwC_CG
Cuts at: 2475 2475
Size: 4280
SacI G_AGCT'C
Cuts at: 1294 1294
Size: 4280
SacII CC_GC'GG
Cuts at: 160 160
Size: 4280
SalI G'TCGA_C
Cuts at: 1317 1317
Size: 4280
SanDI GG'GwC_CC
Cuts at: 700 700
Size: 4280
SapI GCTCTTCn'nnn_
Cuts at: 238 2303 2513 238
Size: 2065 210 2005
Fragments arranged by size:
2065 2005 210
SbfI CC_TGCA'GG
Cuts at: 1327 1327
Size: 4280
ScaI AGT'ACT
Cuts at: 3797 3797
Size: 4280
SexAI A'CCwGG_T
Cuts at: 756 756
Size: 4280
SfcI C'TryA_G
Cuts at: 33 146 865 1017 1090 1323 2008 2976 3167
Size: 113 719 152 73 233 685 968 191
Cuts at: 3167 33
Size: 1146
Fragments arranged by size:
1146 968 719 685 233 191 152 113
73
SfoI GGC'GCC
Cuts at: 1712 1960 1712
Size: 248 4032
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
SmaI CCC'GGG
Cuts at: 205 1302 205
Size: 1097 3183
SmlI C'TyrA_G
Cuts at: 782 836 1162 1435 2350 3064 3326 782
Size: 54 326 273 915 714 262 1736
Fragments arranged by size:
1736 915 714 326 273 262 54
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4280
SphI G_CATG'C
Cuts at: 1333 1833 1333
Size: 500 3780
SrfI GCCC'GGGC
Cuts at: 205 205
Size: 4280
StyI C'CwwG_G
Cuts at: 107 339 423 873 950 1414 1768 107
Size: 232 84 450 77 464 354 2619
Fragments arranged by size:
2619 464 450 354 232 84 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1246 1863 1996 2455 2664 1246
Size: 617 133 459 209 2862
Fragments arranged by size:
2862 617 459 209 133
TatI w'GTAC_w
Cuts at: 3795 3795
Size: 4280
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 469 501 3797 3829 469
Size: 32 3296 32 920
Fragments arranged by size:
3296 920 32 32
Tth111I GACn'n_nGTC
Cuts at: 2077 2077
Size: 4280
XbaI T'CTAG_A
Cuts at: 1311 1311
Size: 4280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 808 1062 1775 808
Size: 254 713 3313
Fragments arranged by size:
3313 713 254
XhoI C'TCGA_G
Cuts at: 836 836
Size: 4280
XmaI C'CCGG_G
Cuts at: 203 1300 203
Size: 1097 3183
XmnI GAAnn'nnTTC
Cuts at: 520 1084 520
Size: 564 3716
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BsgI BsiWI BsmI BspEI BsrGI BssHII BstEII Bsu36I
ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PshAI PsiI PspXI PsrI SfiI SgrAI SnaBI SspI
StuI SwaI ZraI