Restriction map for
pF1KB4374
(Circular) (Six-base) MAPSORT of: pF1KB4374.seq Check: 7881 from: 1 to: 4355
pF1KB4374 4355 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 889 1393 889
Size: 504 3851
Acc65I G'GTAC_C
Cuts at: 1371 1371
Size: 4355
AclI AA'CG_TT
Cuts at: 4195 4195
Size: 4355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2180 2612 3359 3627 2180
Size: 432 747 268 2908
Fragments arranged by size:
2908 747 432 268
AflIII A'CryG_T
Cuts at: 1707 2811 3788 1707
Size: 1104 977 2274
Fragments arranged by size:
2274 1104 977
AgeI A'CCGG_T
Cuts at: 2743 2743
Size: 4355
AlwNI CAG_nnn'CTG
Cuts at: 1660 3227 1660
Size: 1567 2788
ApaLI G'TGCA_C
Cuts at: 3125 3125
Size: 4355
ApoI r'AATT_y
Cuts at: 380 949 992 1110 1345 1359 3708 3964 380
Size: 569 43 118 235 14 2349 256 771
Fragments arranged by size:
2349 771 569 256 235 118 43 14
AseI AT'TA_AT
Cuts at: 20 506 560 20
Size: 486 54 3815
Fragments arranged by size:
3815 486 54
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
AvaI C'yCGr_G
Cuts at: 1375 1375
Size: 4355
BamHI G'GATC_C
Cuts at: 1380 1380
Size: 4355
BanI G'GyrC_C
Cuts at: 1371 1785 2033 2068 1371
Size: 414 248 35 3658
Fragments arranged by size:
3658 414 248 35
BanII G_rGCy'C
Cuts at: 1369 2399 1369
Size: 1030 3325
BbeI G_GCGC'C
Cuts at: 1789 2037 1789
Size: 248 4107
BbsI GAAGACnn'nnnn_
Cuts at: 1034 1034
Size: 4355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 976 1010 976
Size: 34 4321
BciVI GTATCCnnnnn_n'
Cuts at: 2253 3014 4355 2253
Size: 761 1341 2253
Fragments arranged by size:
2253 1341 761
BclI T'GATC_A
Cuts at: 1875 1875
Size: 4355
BglII A'GATC_T
Cuts at: 1870 3907 1870
Size: 2037 2318
BlpI GC'TnA_GC
Cuts at: 983 1467 983
Size: 484 3871
Bme1580I G_kGCm'C
Cuts at: 371 1980 2073 3129 371
Size: 1609 93 1056 1597
Fragments arranged by size:
1609 1597 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 487 690 1567 1648 1983 4127 487
Size: 203 877 81 335 2144 715
Fragments arranged by size:
2144 877 715 335 203 81
BmtI G_CTAG'C
Cuts at: 1171 1171
Size: 4355
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 4355
Bpu10I CC'TnA_GC
Cuts at: 829 829
Size: 4355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1531 2410 2902 3200 1531
Size: 879 492 298 2686
Fragments arranged by size:
2686 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 408 4059 408
Size: 3651 704
BsaAI yAC'GTr
Cuts at: 1708 1708
Size: 4355
BsaBI GATnn'nnATC
Cuts at: 6 1869 3906 6
Size: 1863 2037 455
Fragments arranged by size:
2037 1863 455
BsaHI Gr'CG_yC
Cuts at: 1786 2034 2736 4232 1786
Size: 248 702 1496 1909
Fragments arranged by size:
1909 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1544 2065 2743 3017 3164 3575 1544
Size: 521 678 274 147 411 2324
Fragments arranged by size:
2324 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 151 181 1216 1246 151
Size: 30 1035 30 3260
Fragments arranged by size:
3260 1035 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1188 1345 1188
Size: 157 4198
BseYI C'CCAG_C
Cuts at: 1780 2538 3115 3611 1780
Size: 758 577 496 2524
Fragments arranged by size:
2524 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 610 322
Size: 288 4067
BsiEI CG_ry'CG
Cuts at: 104 1897 1943 3151 104
Size: 1793 46 1208 1308
Fragments arranged by size:
1793 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1369 2147 2337 3129 1369
Size: 778 190 792 2595
Fragments arranged by size:
2595 792 778 190
BsmI GAATG_Cn'
Cuts at: 1147 1262 1147
Size: 115 4240
Bsp1286I G_dGCh'C
Cuts at: 371 1369 1980 2073 2147 2337 2399 3129 371
Size: 998 611 93 74 190 62 730 1597
Fragments arranged by size:
1597 998 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3575 3575
Size: 4355
BspHI T'CATG_A
Cuts at: 3531 3531
Size: 4355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1405 1619 1921 2302 1405
Size: 214 302 381 3458
Fragments arranged by size:
3458 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2647 2701 4185 4350 50
Size: 2597 54 1484 165 55
Fragments arranged by size:
2597 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 124 324 2267 124
Size: 200 1943 2212
Fragments arranged by size:
2212 1943 200
BsrFI r'CCGG_y
Cuts at: 2353 2534 2743 2353
Size: 181 209 3965
Fragments arranged by size:
3965 209 181
BssSI C'ACGA_G
Cuts at: 2626 2984 2626
Size: 358 3997
BstAPI GCAn_nnn'nTGC
Cuts at: 1732 1732
Size: 4355
BstBI TT'CG_AA
Cuts at: 1151 2716 1151
Size: 1565 2790
BstXI CCAn_nnnn'nTGG
Cuts at: 477 477
Size: 4355
BstYI r'GATC_y
Cuts at: 1 1278 1380 1847 1870 2205 2451 3463 3549
Size: 1277 102 467 23 335 246 1012 86
Cuts at: 3549 3561 3907 1
Size: 12 346 449
Fragments arranged by size:
1277 1012 467 449 346 335 246 102
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 667 667
Size: 4355
BtgI C'CryG_G
Cuts at: 155 1274 155
Size: 1119 3236
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2305 2486 2649 2305
Size: 181 163 4011
Fragments arranged by size:
4011 181 163
BtsI GCAGTG_nn'
Cuts at: 1598 1598
Size: 4355
DraI TTT'AAA
Cuts at: 569 1354 569
Size: 785 3570
DrdI GACnn_nn'nnGTC
Cuts at: 2061 2919 2061
Size: 858 3497
EaeI y'GGCC_r
Cuts at: 585 1940 2114 2505 2532 3877 585
Size: 1355 174 391 27 1345 1063
Fragments arranged by size:
1355 1345 1063 391 174 27
EagI C'GGCC_G
Cuts at: 1940 1940
Size: 4355
EarI CTCTTCn'nnn_
Cuts at: 194 2378 2588 194
Size: 2184 210 1961
Fragments arranged by size:
2184 1961 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2563 2873 3019 4165 2563
Size: 310 146 1146 2753
Fragments arranged by size:
2753 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1367 1367
Size: 4355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2180 2612 3359 3627 2180
Size: 432 747 268 2908
Fragments arranged by size:
2908 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 200 1141 200
Size: 941 3414
EcoO109I rG'GnC_Cy
Cuts at: 205 909 1020 1494 205
Size: 704 111 474 3066
Fragments arranged by size:
3066 704 474 111
EcoRI G'AATT_C
Cuts at: 380 1359 380
Size: 979 3376
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 789 2374 789
Size: 1585 2770
FspI TGC'GCA
Cuts at: 2136 2136
Size: 4355
HaeII r_GCGC'y
Cuts at: 1789 2037 3059 4038 1789
Size: 248 1022 979 2106
Fragments arranged by size:
2106 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 183 203 235 1051 1083 2197 2227 2229
Size: 32 20 32 816 32 1114 30 2
Cuts at: 2229 2259 151
Size: 30 2247
Fragments arranged by size:
2247 1114 816 32 32 32 30 30
20 2
HincII GTy'rAC
Cuts at: 1394 1394
Size: 4355
HindIII A'AGCT_T
Cuts at: 766 794 1215 766
Size: 28 421 3906
Fragments arranged by size:
3906 421 28
KasI G'GCGC_C
Cuts at: 1785 2033 1785
Size: 248 4107
KpnI G_GTAC'C
Cuts at: 1375 1375
Size: 4355
MluI A'CGCG_T
Cuts at: 3788 3788
Size: 4355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 221 323 3026 3210 221
Size: 102 2703 184 1366
Fragments arranged by size:
2703 1366 184 102
MscI TGG'CCA
Cuts at: 587 2116 587
Size: 1529 2826
MslI CAynn'nnrTG
Cuts at: 520 2344 2471 520
Size: 1824 127 2404
Fragments arranged by size:
2404 1824 127
MspA1I CmG'CkG
Cuts at: 1466 1780 2140 3153 3398 1466
Size: 314 360 1013 245 2423
Fragments arranged by size:
2423 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2536 2536
Size: 4355
NarI GG'CG_CC
Cuts at: 1786 2034 1786
Size: 248 4107
NgoMIV G'CCGG_C
Cuts at: 2534 2534
Size: 4355
NheI G'CTAG_C
Cuts at: 1167 1167
Size: 4355
NspI r_CATG'y
Cuts at: 1408 1908 2815 1408
Size: 500 907 2948
Fragments arranged by size:
2948 907 500
PasI CC'CwG_GG
Cuts at: 1137 1137
Size: 4355
PciI A'CATG_T
Cuts at: 2811 2811
Size: 4355
PflMI CCAn_nnn'nTGG
Cuts at: 113 228 279 113
Size: 115 51 4189
Fragments arranged by size:
4189 115 51
PmeI GTTT'AAAC
Cuts at: 1354 1354
Size: 4355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 3494
Size: 32 4323
PpuMI rG'GwC_Cy
Cuts at: 205 909 1020 205
Size: 704 111 3540
Fragments arranged by size:
3540 704 111
PstI C_TGCA'G
Cuts at: 1402 2087 1402
Size: 685 3670
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4355
PvuII CAG'CTG
Cuts at: 1780 2140 1780
Size: 360 3995
RsrII CG'GwC_CG
Cuts at: 2550 2550
Size: 4355
SacI G_AGCT'C
Cuts at: 1369 1369
Size: 4355
SalI G'TCGA_C
Cuts at: 1392 1392
Size: 4355
SapI GCTCTTCn'nnn_
Cuts at: 2378 2588 2378
Size: 210 4145
SbfI CC_TGCA'GG
Cuts at: 1402 1402
Size: 4355
ScaI AGT'ACT
Cuts at: 745 3872 745
Size: 3127 1228
SfcI C'TryA_G
Cuts at: 33 385 449 890 1398 2083 3076 3267 33
Size: 352 64 441 508 685 993 191 1121
Fragments arranged by size:
1121 993 685 508 441 352 191 64
SfoI GGC'GCC
Cuts at: 1787 2035 1787
Size: 248 4107
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
SmaI CCC'GGG
Cuts at: 1377 1377
Size: 4355
SmlI C'TyrA_G
Cuts at: 1510 2425 2917 3179 1510
Size: 915 492 262 2686
Fragments arranged by size:
2686 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4355
SphI G_CATG'C
Cuts at: 1408 1908 1408
Size: 500 3855
StyI C'CwwG_G
Cuts at: 1489 1843 1489
Size: 354 4001
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 644 773 1938 2071 2530 2739 644
Size: 129 1165 133 459 209 2260
Fragments arranged by size:
2260 1165 459 209 133 129
TatI w'GTAC_w
Cuts at: 743 3870 743
Size: 3127 1228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3872 3904 3872
Size: 32 4323
Tth111I GACn'n_nGTC
Cuts at: 2152 2152
Size: 4355
XbaI T'CTAG_A
Cuts at: 1386 1386
Size: 4355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1850 1850
Size: 4355
XmaI C'CCGG_G
Cuts at: 1375 1375
Size: 4355
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NspI PasI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BpmI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI