Restriction map for
pF1KB4372
(Circular) (Six-base) MAPSORT of: pF1KB4372.seq Check: 9213 from: 1 to: 5294
pF1KB4372 5294 bp
With 182 enzymes: SgfI *
November 16, 2012 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1396 1396
Size: 5294
AccI GT'mk_AC
Cuts at: 758 2332 758
Size: 1574 3720
Acc65I G'GTAC_C
Cuts at: 2310 2310
Size: 5294
AclI AA'CG_TT
Cuts at: 5134 5134
Size: 5294
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1193 1958 3119 3551 4298 4566 1193
Size: 765 1161 432 747 268 1921
Fragments arranged by size:
1921 1161 765 747 432 268
AflIII A'CryG_T
Cuts at: 2646 3750 4727 2646
Size: 1104 977 3213
Fragments arranged by size:
3213 1104 977
AgeI A'CCGG_T
Cuts at: 3682 3682
Size: 5294
AleI CACnn'nnGTG
Cuts at: 1750 1750
Size: 5294
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 255 1047 255
Size: 792 4502
AlwNI CAG_nnn'CTG
Cuts at: 291 2599 4166 291
Size: 2308 1567 1419
Fragments arranged by size:
2308 1567 1419
ApaLI G'TGCA_C
Cuts at: 4064 4064
Size: 5294
ApoI r'AATT_y
Cuts at: 313 940 1693 2105 2155 2298 4647 4903 313
Size: 627 753 412 50 143 2349 256 704
Fragments arranged by size:
2349 753 704 627 412 256 143 50
AseI AT'TA_AT
Cuts at: 20 20
Size: 5294
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5294
AvaI C'yCGr_G
Cuts at: 2314 2314
Size: 5294
BamHI G'GATC_C
Cuts at: 2319 2319
Size: 5294
BanI G'GyrC_C
Cuts at: 2310 2724 2972 3007 2310
Size: 414 248 35 4597
Fragments arranged by size:
4597 414 248 35
BanII G_rGCy'C
Cuts at: 708 1537 2308 3338 708
Size: 829 771 1030 2664
Fragments arranged by size:
2664 1030 829 771
BbeI G_GCGC'C
Cuts at: 2728 2976 2728
Size: 248 5046
BbsI GAAGACnn'nnnn_
Cuts at: 877 877
Size: 5294
BbvCI CC'TCA_GC
Cuts at: 691 691
Size: 5294
BciVI GTATCCnnnnn_n'
Cuts at: 3192 3953 5294 3192
Size: 761 1341 3192
Fragments arranged by size:
3192 1341 761
BclI T'GATC_A
Cuts at: 2814 2814
Size: 5294
BglI GCCn_nnn'nGGC
Cuts at: 1639 1639
Size: 5294
BglII A'GATC_T
Cuts at: 2809 4846 2809
Size: 2037 3257
BlpI GC'TnA_GC
Cuts at: 284 2406 284
Size: 2122 3172
Bme1580I G_kGCm'C
Cuts at: 2919 3012 4068 2919
Size: 93 1056 4145
Fragments arranged by size:
4145 1056 93
BmgBI CAC'GTC
Cuts at: 948 1768 948
Size: 820 4474
BmrI ACTGGGnnnn_n'
Cuts at: 636 2506 2587 2922 5066 636
Size: 1870 81 335 2144 864
Fragments arranged by size:
2144 1870 864 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3661 3661
Size: 5294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 712
Size: 5294
Bpu10I CC'TnA_GC
Cuts at: 691 691
Size: 5294
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 2470 3349 3841 4139 850
Size: 1620 879 492 298 2005
Fragments arranged by size:
2005 1620 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4998 4998
Size: 5294
BsaAI yAC'GTr
Cuts at: 2647 2647
Size: 5294
BsaBI GATnn'nnATC
Cuts at: 6 1156 2808 4845 6
Size: 1150 1652 2037 455
Fragments arranged by size:
2037 1652 1150 455
BsaHI Gr'CG_yC
Cuts at: 2725 2973 3675 5171 2725
Size: 248 702 1496 2848
Fragments arranged by size:
2848 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2483 3004 3682 3956 4103 4514 2483
Size: 521 678 274 147 411 3263
Fragments arranged by size:
3263 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 740 770 1161 1191 2273 2303 740
Size: 30 391 30 1082 30 3731
Fragments arranged by size:
3731 1082 391 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 164 350 353 164
Size: 186 3 5105
Fragments arranged by size:
5105 186 3
BseYI C'CCAG_C
Cuts at: 1104 2719 3477 4054 4550 1104
Size: 1615 758 577 496 1848
Fragments arranged by size:
1848 1615 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1579 1728 1579
Size: 149 5145
BsiEI CG_ry'CG
Cuts at: 104 1160 2836 2882 4090 104
Size: 1056 1676 46 1208 1308
Fragments arranged by size:
1676 1308 1208 1056 46
BsiHKAI G_wGCw'C
Cuts at: 159 2308 3086 3276 4068 159
Size: 2149 778 190 792 1385
Fragments arranged by size:
2149 1385 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 159 708 1537 2308 2919 3012 3086 3276 3338
Size: 549 829 771 611 93 74 190 62
Cuts at: 3338 4068 159
Size: 730 1385
Fragments arranged by size:
1385 829 771 730 611 549 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4514 4514
Size: 5294
BspHI T'CATG_A
Cuts at: 485 531 4470 485
Size: 46 3939 1309
Fragments arranged by size:
3939 1309 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 554 1396 2344 2558 2860 3241 554
Size: 842 948 214 302 381 2607
Fragments arranged by size:
2607 948 842 381 302 214
BsrBI CCG'CTC
Cuts at: 50 905 3586 3640 5124 5289 50
Size: 855 2681 54 1484 165 55
Fragments arranged by size:
2681 1484 855 165 55 54
BsrDI GCAATG_nn'
Cuts at: 577 800 3206 577
Size: 223 2406 2665
Fragments arranged by size:
2665 2406 223
BsrFI r'CCGG_y
Cuts at: 2204 3292 3473 3682 2204
Size: 1088 181 209 3816
Fragments arranged by size:
3816 1088 209 181
BssSI C'ACGA_G
Cuts at: 3565 3923 3565
Size: 358 4936
BstAPI GCAn_nnn'nTGC
Cuts at: 2671 2671
Size: 5294
BstBI TT'CG_AA
Cuts at: 3655 3655
Size: 5294
BstXI CCAn_nnnn'nTGG
Cuts at: 846 846
Size: 5294
BstYI r'GATC_y
Cuts at: 1 2319 2786 2809 3144 3390 4402 4488 4500
Size: 2318 467 23 335 246 1012 86 12
Cuts at: 4500 4846 1
Size: 346 449
Fragments arranged by size:
2318 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 453 974 107
Size: 346 521 4427
Fragments arranged by size:
4427 521 346
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3244 3425 3588 3244
Size: 181 163 4950
Fragments arranged by size:
4950 181 163
BtsI GCAGTG_nn'
Cuts at: 131 1745 2537 131
Size: 1614 792 2888
Fragments arranged by size:
2888 1614 792
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2246 2281 2246
Size: 35 5259
DraI TTT'AAA
Cuts at: 1478 2293 1478
Size: 815 4479
DrdI GACnn_nn'nnGTC
Cuts at: 3000 3858 3000
Size: 858 4436
EaeI y'GGCC_r
Cuts at: 1631 2879 3053 3444 3471 4816 1631
Size: 1248 174 391 27 1345 2109
Fragments arranged by size:
2109 1345 1248 391 174 27
EagI C'GGCC_G
Cuts at: 2879 2879
Size: 5294
EarI CTCTTCn'nnn_
Cuts at: 1272 1990 2228 2244 3317 3527 1272
Size: 718 238 16 1073 210 3039
Fragments arranged by size:
3039 1073 718 238 210 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3502 3812 3958 5104 3502
Size: 310 146 1146 3692
Fragments arranged by size:
3692 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2306 2306
Size: 5294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1193 1958 3119 3551 4298 4566 712
Size: 481 765 1161 432 747 268 1440
Fragments arranged by size:
1440 1161 765 747 481 432 268
EcoO109I rG'GnC_Cy
Cuts at: 979 2433 979
Size: 1454 3840
EcoRI G'AATT_C
Cuts at: 2298 2298
Size: 5294
EcoRV GAT'ATC
Cuts at: 321 469 1221 1377 321
Size: 148 752 156 4238
Fragments arranged by size:
4238 752 156 148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 544 3313 544
Size: 2769 2525
FspI TGC'GCA
Cuts at: 1000 3075 1000
Size: 2075 3219
HaeII r_GCGC'y
Cuts at: 2728 2976 3998 4977 2728
Size: 248 1022 979 3045
Fragments arranged by size:
3045 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 400 403 432 435 740 772 1271 1303 1318
Size: 3 29 3 305 32 499 32 15
Cuts at: 1318 1333 1350 1365 1410 1442 1752 1784 3136
Size: 15 17 15 45 32 310 32 1352
Cuts at: 3136 3166 3168 3198 400
Size: 30 2 30 2496
Fragments arranged by size:
2496 1352 499 310 305 45 32 32
32 32 30 30 29 17 15 15
15 3 3 2
HincII GTy'rAC
Cuts at: 258 1943 2333 258
Size: 1685 390 3219
Fragments arranged by size:
3219 1685 390
HpaI GTT'AAC
Cuts at: 258 1943 258
Size: 1685 3609
KasI G'GCGC_C
Cuts at: 2724 2972 2724
Size: 248 5046
KpnI G_GTAC'C
Cuts at: 2314 2314
Size: 5294
MfeI C'AATT_G
Cuts at: 666 752 666
Size: 86 5208
MluI A'CGCG_T
Cuts at: 4727 4727
Size: 5294
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 126 473 1204 1653 3965 4149 126
Size: 347 731 449 2312 184 1271
Fragments arranged by size:
2312 1271 731 449 347 184
MscI TGG'CCA
Cuts at: 1633 3055 1633
Size: 1422 3872
MslI CAynn'nnrTG
Cuts at: 458 536 779 1750 3283 3410 458
Size: 78 243 971 1533 127 2342
Fragments arranged by size:
2342 1533 971 243 127 78
MspA1I CmG'CkG
Cuts at: 2166 2405 2719 3079 4092 4337 2166
Size: 239 314 360 1013 245 3123
Fragments arranged by size:
3123 1013 360 314 245 239
NaeI GCC'GGC
Cuts at: 2206 3475 2206
Size: 1269 4025
NarI GG'CG_CC
Cuts at: 2725 2973 2725
Size: 248 5046
NcoI C'CATG_G
Cuts at: 107 453 107
Size: 346 4948
NgoMIV G'CCGG_C
Cuts at: 2204 3473 2204
Size: 1269 4025
NspI r_CATG'y
Cuts at: 901 2347 2847 3754 901
Size: 1446 500 907 2441
Fragments arranged by size:
2441 1446 907 500
PciI A'CATG_T
Cuts at: 3750 3750
Size: 5294
PflMI CCAn_nnn'nTGG
Cuts at: 1045 1045
Size: 5294
PmeI GTTT'AAAC
Cuts at: 2293 2293
Size: 5294
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4433 4465 4433
Size: 32 5262
PpuMI rG'GwC_Cy
Cuts at: 979 979
Size: 5294
PsiI TTA'TAA
Cuts at: 1865 1865
Size: 5294
PstI C_TGCA'G
Cuts at: 476 1341 2232 2341 3026 476
Size: 865 891 109 685 2744
Fragments arranged by size:
2744 891 865 685 109
PvuI CG_AT'CG
Cuts at: 104 1160 104
Size: 1056 4238
PvuII CAG'CTG
Cuts at: 2166 2719 3079 2166
Size: 553 360 4381
Fragments arranged by size:
4381 553 360
RsrII CG'GwC_CG
Cuts at: 3489 3489
Size: 5294
SacI G_AGCT'C
Cuts at: 2308 2308
Size: 5294
SalI G'TCGA_C
Cuts at: 2331 2331
Size: 5294
SapI GCTCTTCn'nnn_
Cuts at: 3317 3527 3317
Size: 210 5084
SbfI CC_TGCA'GG
Cuts at: 2341 2341
Size: 5294
ScaI AGT'ACT
Cuts at: 4811 4811
Size: 5294
SfcI C'TryA_G
Cuts at: 33 472 1337 1400 2228 2337 3022 4015 4206
Size: 439 865 63 828 109 685 993 191
Cuts at: 4206 33
Size: 1121
Fragments arranged by size:
1121 993 865 828 685 439 191 109
63
SfoI GGC'GCC
Cuts at: 2726 2974 2726
Size: 248 5046
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5294
SmaI CCC'GGG
Cuts at: 2316 2316
Size: 5294
SmlI C'TyrA_G
Cuts at: 865 2449 3364 3856 4118 865
Size: 1584 915 492 262 2041
Fragments arranged by size:
2041 1584 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5294
SphI G_CATG'C
Cuts at: 2347 2847 2347
Size: 500 4794
StuI AGG'CCT
Cuts at: 1621 1621
Size: 5294
StyI C'CwwG_G
Cuts at: 107 453 2428 2782 107
Size: 346 1975 354 2619
Fragments arranged by size:
2619 1975 354 346
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1418 2877 3010 3469 3678 1418
Size: 1459 133 459 209 3034
Fragments arranged by size:
3034 1459 459 209 133
TatI w'GTAC_w
Cuts at: 4809 4809
Size: 5294
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4811 4843 4811
Size: 32 5262
Tth111I GACn'n_nGTC
Cuts at: 3091 3091
Size: 5294
XbaI T'CTAG_A
Cuts at: 2325 2325
Size: 5294
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 489 602 1042 2789 489
Size: 113 440 1747 2994
Fragments arranged by size:
2994 1747 440 113
XmaI C'CCGG_G
Cuts at: 2314 2314
Size: 5294
XmnI GAAnn'nnTTC
Cuts at: 527 527
Size: 5294
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI FseI
FspAI HindIII NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI