Restriction map for
pF1KB4371
(Circular) (Six-base) MAPSORT of: pF1KB4371.seq Check: 3743 from: 1 to: 3587
pF1KB4371 3587 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 625 625
Size: 3587
Acc65I G'GTAC_C
Cuts at: 183 603 183
Size: 420 3167
AclI AA'CG_TT
Cuts at: 3427 3427
Size: 3587
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1412 1844 2197 2859 1412
Size: 432 353 662 2140
Fragments arranged by size:
2140 662 432 353
AfeI AGC'GCT
Cuts at: 415 415
Size: 3587
AflIII A'CryG_T
Cuts at: 573 939 2739 3020 573
Size: 366 1800 281 1140
Fragments arranged by size:
1800 1140 366 281
AlwNI CAG_nnn'CTG
Cuts at: 892 2330 892
Size: 1438 2149
ApaLI G'TGCA_C
Cuts at: 2425 2425
Size: 3587
ApoI r'AATT_y
Cuts at: 591 2940 3196 591
Size: 2349 256 982
Fragments arranged by size:
2349 982 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3587
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3587
AvaI C'yCGr_G
Cuts at: 607 607
Size: 3587
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 167 200 167
Size: 33 3554
BamHI G'GATC_C
Cuts at: 612 612
Size: 3587
BanI G'GyrC_C
Cuts at: 183 603 1017 1265 1300 183
Size: 420 414 248 35 2470
Fragments arranged by size:
2470 420 414 248 35
BanII G_rGCy'C
Cuts at: 601 1631 601
Size: 1030 2557
BbeI G_GCGC'C
Cuts at: 1021 1269 1021
Size: 248 3339
BbsI GAAGACnn'nnnn_
Cuts at: 224 224
Size: 3587
BciVI GTATCCnnnnn_n'
Cuts at: 1485 2541 3587 1485
Size: 1056 1046 1485
Fragments arranged by size:
1485 1056 1046
BclI T'GATC_A
Cuts at: 1107 1107
Size: 3587
BglII A'GATC_T
Cuts at: 1102 3139 1102
Size: 2037 1550
BlpI GC'TnA_GC
Cuts at: 699 699
Size: 3587
Bme1580I G_kGCm'C
Cuts at: 479 1212 1305 2429 479
Size: 733 93 1124 1637
Fragments arranged by size:
1637 1124 733 93
BmrI ACTGGGnnnn_n'
Cuts at: 238 799 880 1215 3359 238
Size: 561 81 335 2144 466
Fragments arranged by size:
2144 561 466 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1954 1954
Size: 3587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 427 458 551 104
Size: 323 31 93 3140
Fragments arranged by size:
3140 323 93 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 1642 2356 2654 763
Size: 879 714 298 1696
Fragments arranged by size:
1696 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3291 3291
Size: 3587
BsaAI yAC'GTr
Cuts at: 940 940
Size: 3587
BsaBI GATnn'nnATC
Cuts at: 6 319 1101 3138 6
Size: 313 782 2037 455
Fragments arranged by size:
2037 782 455 313
BsaHI Gr'CG_yC
Cuts at: 1018 1266 1968 3464 1018
Size: 248 702 1496 1141
Fragments arranged by size:
1496 1141 702 248
BsaWI w'CCGG_w
Cuts at: 776 1297 1975 2386 2533 2807 776
Size: 521 678 411 147 274 1556
Fragments arranged by size:
1556 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 424 424
Size: 3587
BseYI C'CCAG_C
Cuts at: 1012 1770 2435 2843 1012
Size: 758 665 408 1756
Fragments arranged by size:
1756 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 349
Size: 3587
BsiEI CG_ry'CG
Cuts at: 104 1129 1175 2405 104
Size: 1025 46 1230 1286
Fragments arranged by size:
1286 1230 1025 46
BsiHKAI G_wGCw'C
Cuts at: 311 601 1379 1569 2429 311
Size: 290 778 190 860 1469
Fragments arranged by size:
1469 860 778 290 190
Bsp1286I G_dGCh'C
Cuts at: 311 479 601 1212 1305 1379 1569 1631 2429
Size: 168 122 611 93 74 190 62 798
Cuts at: 2429 311
Size: 1469
Fragments arranged by size:
1469 798 611 190 168 122 93 74
62
BspHI T'CATG_A
Cuts at: 2019 2019
Size: 3587
BspMI ACCTGCnnnn'nnnn_
Cuts at: 637 851 1153 1534 637
Size: 214 302 381 2690
Fragments arranged by size:
2690 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1879 1933 3417 3582 50
Size: 1829 54 1484 165 55
Fragments arranged by size:
1829 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1499 1499
Size: 3587
BsrFI r'CCGG_y
Cuts at: 1585 1766 1585
Size: 181 3406
BssSI C'ACGA_G
Cuts at: 1858 2566 1858
Size: 708 2879
BstAPI GCAn_nnn'nTGC
Cuts at: 964 964
Size: 3587
BstBI TT'CG_AA
Cuts at: 1948 1948
Size: 3587
BstYI r'GATC_y
Cuts at: 1 612 1079 1102 1437 1683 1989 2001 2087
Size: 611 467 23 335 246 306 12 86
Cuts at: 2087 3139 1
Size: 1052 449
Fragments arranged by size:
1052 611 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 518 518
Size: 3587
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1537 1718 1881 1537
Size: 181 163 3243
Fragments arranged by size:
3243 181 163
BtsI GCAGTG_nn'
Cuts at: 830 830
Size: 3587
DraI TTT'AAA
Cuts at: 586 586
Size: 3587
DrdI GACnn_nn'nnGTC
Cuts at: 1293 2637 1293
Size: 1344 2243
EaeI y'GGCC_r
Cuts at: 1172 1346 1737 1764 3109 1172
Size: 174 391 27 1345 1650
Fragments arranged by size:
1650 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1172 1172
Size: 3587
EarI CTCTTCn'nnn_
Cuts at: 148 1610 1820 148
Size: 1462 210 1915
Fragments arranged by size:
1915 1462 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1795 2537 2683 3397 1795
Size: 742 146 714 1985
Fragments arranged by size:
1985 742 714 146
EcoICRI GAG'CTC
Cuts at: 599 599
Size: 3587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 427 458 551 1412 1844 2197 2859 104
Size: 323 31 93 861 432 353 662 832
Fragments arranged by size:
861 832 662 432 353 323 93 31
EcoNI CCTnn'n_nnAGG
Cuts at: 406 406
Size: 3587
EcoO109I rG'GnC_Cy
Cuts at: 433 514 726 433
Size: 81 212 3294
Fragments arranged by size:
3294 212 81
EcoRI G'AATT_C
Cuts at: 591 591
Size: 3587
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1606 1606
Size: 3587
FspI TGC'GCA
Cuts at: 1368 1368
Size: 3587
HaeII r_GCGC'y
Cuts at: 417 1021 1269 2499 3270 417
Size: 604 248 1230 771 734
Fragments arranged by size:
1230 771 734 604 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1429 1459 1461 1491 1429
Size: 30 2 30 3525
Fragments arranged by size:
3525 30 30 2
HincII GTy'rAC
Cuts at: 626 626
Size: 3587
KasI G'GCGC_C
Cuts at: 1017 1265 1017
Size: 248 3339
KpnI G_GTAC'C
Cuts at: 187 607 187
Size: 420 3167
MluI A'CGCG_T
Cuts at: 3020 3020
Size: 3587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2346 2530 2346
Size: 184 3403
MscI TGG'CCA
Cuts at: 1348 1348
Size: 3587
MslI CAynn'nnrTG
Cuts at: 325 1576 1703 325
Size: 1251 127 2209
Fragments arranged by size:
2209 1251 127
MspA1I CmG'CkG
Cuts at: 698 1012 1372 2156 2401 698
Size: 314 360 784 245 1884
Fragments arranged by size:
1884 784 360 314 245
NaeI GCC'GGC
Cuts at: 1768 1768
Size: 3587
NarI GG'CG_CC
Cuts at: 1018 1266 1018
Size: 248 3339
NgoMIV G'CCGG_C
Cuts at: 1766 1766
Size: 3587
NspI r_CATG'y
Cuts at: 577 640 1140 2743 577
Size: 63 500 1603 1421
Fragments arranged by size:
1603 1421 500 63
PciI A'CATG_T
Cuts at: 573 2739 573
Size: 2166 1421
PflMI CCAn_nnn'nTGG
Cuts at: 228 303 228
Size: 75 3512
PfoI T'CCnGG_A
Cuts at: 567 567
Size: 3587
PmeI GTTT'AAAC
Cuts at: 586 586
Size: 3587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2033 2065 2033
Size: 32 3555
PpuMI rG'GwC_Cy
Cuts at: 514 514
Size: 3587
PstI C_TGCA'G
Cuts at: 634 1319 634
Size: 685 2902
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3587
PvuII CAG'CTG
Cuts at: 1012 1372 1012
Size: 360 3227
RsrII CG'GwC_CG
Cuts at: 1782 1782
Size: 3587
SacI G_AGCT'C
Cuts at: 601 601
Size: 3587
SalI G'TCGA_C
Cuts at: 624 624
Size: 3587
SapI GCTCTTCn'nnn_
Cuts at: 148 1610 1820 148
Size: 1462 210 1915
Fragments arranged by size:
1915 1462 210
SbfI CC_TGCA'GG
Cuts at: 634 634
Size: 3587
ScaI AGT'ACT
Cuts at: 3104 3104
Size: 3587
SfcI C'TryA_G
Cuts at: 33 345 630 1315 2283 2474 33
Size: 312 285 685 968 191 1146
Fragments arranged by size:
1146 968 685 312 285 191
SfoI GGC'GCC
Cuts at: 1019 1267 1019
Size: 248 3339
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3587
SmaI CCC'GGG
Cuts at: 609 609
Size: 3587
SmlI C'TyrA_G
Cuts at: 742 1657 2371 2633 742
Size: 915 714 262 1696
Fragments arranged by size:
1696 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3587
SphI G_CATG'C
Cuts at: 640 1140 640
Size: 500 3087
StuI AGG'CCT
Cuts at: 483 483
Size: 3587
StyI C'CwwG_G
Cuts at: 179 302 721 1075 179
Size: 123 419 354 2691
Fragments arranged by size:
2691 419 354 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1170 1303 1762 1971 1170
Size: 133 459 209 2786
Fragments arranged by size:
2786 459 209 133
TatI w'GTAC_w
Cuts at: 3102 3102
Size: 3587
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3104 3136 3104
Size: 32 3555
Tth111I GACn'n_nGTC
Cuts at: 1384 1384
Size: 3587
XbaI T'CTAG_A
Cuts at: 618 618
Size: 3587
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1082 1082
Size: 3587
XmaI C'CCGG_G
Cuts at: 607 607
Size: 3587
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI