Restriction map for
pF1KB4370
(Circular) (Six-base) MAPSORT of: pF1KB4370.seq Check: 2353 from: 1 to: 8468
pF1KB4370 8468 bp
With 182 enzymes: SgfI *
July 1, 2016 17:01 ..
AatII G_ACGT'C
Cuts at: 1957 1957
Size: 8468
AccI GT'mk_AC
Cuts at: 3343 4719 5506 3343
Size: 1376 787 6305
Fragments arranged by size:
6305 1376 787
Acc65I G'GTAC_C
Cuts at: 5436 5484 5436
Size: 48 8420
AclI AA'CG_TT
Cuts at: 8308 8308
Size: 8468
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1430 2873 3202 4520 6293 6725 7472 7740 1430
Size: 1443 329 1318 1773 432 747 268 2158
Fragments arranged by size:
2158 1773 1443 1318 747 432 329 268
AflIII A'CryG_T
Cuts at: 601 4557 4922 5820 6924 7901 601
Size: 3956 365 898 1104 977 1168
Fragments arranged by size:
3956 1168 1104 977 898 365
AgeI A'CCGG_T
Cuts at: 3127 6856 3127
Size: 3729 4739
AhdI GACnn_n'nnGTC
Cuts at: 1140 4165 4635 1140
Size: 3025 470 4973
Fragments arranged by size:
4973 3025 470
AleI CACnn'nnGTG
Cuts at: 2854 5457 2854
Size: 2603 5865
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1241 4868 1241
Size: 3627 4841
AlwNI CAG_nnn'CTG
Cuts at: 212 675 949 1045 1453 1614 3808 4079 4227
Size: 463 274 96 408 161 2194 271 148
Cuts at: 4227 4740 5773 7340 212
Size: 513 1033 1567 1340
Fragments arranged by size:
2194 1567 1340 1033 513 463 408 274
271 161 148 96
ApaI G_GGCC'C
Cuts at: 150 493 150
Size: 343 8125
ApaLI G'TGCA_C
Cuts at: 7238 7238
Size: 8468
ApoI r'AATT_y
Cuts at: 566 2269 3066 4489 5208 5472 7821 8077 566
Size: 1703 797 1423 719 264 2349 256 957
Fragments arranged by size:
2349 1703 1423 957 797 719 264 256
AseI AT'TA_AT
Cuts at: 20 2341 4127 20
Size: 2321 1786 4361
Fragments arranged by size:
4361 2321 1786
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8468
AvaI C'yCGr_G
Cuts at: 143 1977 4022 5488 143
Size: 1834 2045 1466 3123
Fragments arranged by size:
3123 2045 1834 1466
BamHI G'GATC_C
Cuts at: 2090 3485 5099 5493 2090
Size: 1395 1614 394 5065
Fragments arranged by size:
5065 1614 1395 394
BanI G'GyrC_C
Cuts at: 5436 5484 5898 6146 6181 5436
Size: 48 414 248 35 7723
Fragments arranged by size:
7723 414 248 48 35
BanII G_rGCy'C
Cuts at: 150 435 493 963 1469 1778 3110 5360 5482
Size: 285 58 470 506 309 1332 2250 122
Cuts at: 5482 6512 150
Size: 1030 2106
Fragments arranged by size:
2250 2106 1332 1030 506 470 309 285
122 58
BbeI G_GCGC'C
Cuts at: 5902 6150 5902
Size: 248 8220
BbsI GAAGACnn'nnnn_
Cuts at: 2141 3142 4919 2141
Size: 1001 1777 5690
Fragments arranged by size:
5690 1777 1001
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2885 2919 90
Size: 34 2761 34 5639
Fragments arranged by size:
5639 2761 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 795 5408 6366 7127 8468 795
Size: 4613 958 761 1341 795
Fragments arranged by size:
4613 1341 958 795 761
BclI T'GATC_A
Cuts at: 3810 5988 3810
Size: 2178 6290
BfrBI ATG'CAT
Cuts at: 3092 3092
Size: 8468
BglI GCCn_nnn'nGGC
Cuts at: 3185 3185
Size: 8468
BglII A'GATC_T
Cuts at: 2532 3080 5983 8020 2532
Size: 548 2903 2037 2980
Fragments arranged by size:
2980 2903 2037 548
BlpI GC'TnA_GC
Cuts at: 1777 3113 5580 1777
Size: 1336 2467 4665
Fragments arranged by size:
4665 2467 1336
Bme1580I G_kGCm'C
Cuts at: 150 404 493 6093 6186 7242 150
Size: 254 89 5600 93 1056 1376
Fragments arranged by size:
5600 1376 1056 254 93 89
BmrI ACTGGGnnnn_n'
Cuts at: 750 1632 3347 4344 5680 5761 6096 8240 750
Size: 882 1715 997 1336 81 335 2144 978
Fragments arranged by size:
2144 1715 1336 997 978 882 335 81
BmtI G_CTAG'C
Cuts at: 331 331
Size: 8468
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2568 3315 6835 2568
Size: 747 3520 4201
Fragments arranged by size:
4201 3520 747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 1087 1271 1819 2086 2336 2818 2964 3124
Size: 915 184 548 267 250 482 146 160
Cuts at: 3124 172
Size: 5516
Fragments arranged by size:
5516 915 548 482 267 250 184 160
146
Bpu10I CC'TnA_GC
Cuts at: 1730 2009 3765 4271 5354 1730
Size: 279 1756 506 1083 4844
Fragments arranged by size:
4844 1756 1083 506 279
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1804 2538 3634 5644 6523 7015 7313 1804
Size: 734 1096 2010 879 492 298 2959
Fragments arranged by size:
2959 2010 1096 879 734 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3286 3506 3698 4569 8172 3286
Size: 220 192 871 3603 3582
Fragments arranged by size:
3603 3582 871 220 192
BsaAI yAC'GTr
Cuts at: 2241 5821 2241
Size: 3580 4888
BsaBI GATnn'nnATC
Cuts at: 6 1390 4622 4658 5982 8019 6
Size: 1384 3232 36 1324 2037 455
Fragments arranged by size:
3232 2037 1384 1324 455 36
BsaHI Gr'CG_yC
Cuts at: 1439 1954 2803 5899 6147 6849 8345 1439
Size: 515 849 3096 248 702 1496 1562
Fragments arranged by size:
3096 1562 1496 849 702 515 248
BsaWI w'CCGG_w
Cuts at: 3127 5657 6178 6856 7130 7277 7688 3127
Size: 2530 521 678 274 147 411 3907
Fragments arranged by size:
3907 2530 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2215 2245 2215
Size: 30 8438
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1330 2176 4744 1330
Size: 846 2568 5054
Fragments arranged by size:
5054 2568 846
BseYI C'CCAG_C
Cuts at: 188 197 1752 2118 4859 4931 5893 6651 7228
Size: 9 1555 366 2741 72 962 758 577
Cuts at: 7228 7724 188
Size: 496 932
Fragments arranged by size:
2741 1555 962 932 758 577 496 366
72 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 1649 2137 257
Size: 1392 488 6588
Fragments arranged by size:
6588 1392 488
BsiEI CG_ry'CG
Cuts at: 104 6010 6056 7264 104
Size: 5906 46 1208 1308
Fragments arranged by size:
5906 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1469 1592 1778 3110 4847 5360 5482 6260 6450
Size: 123 186 1332 1737 513 122 778 190
Cuts at: 6450 7242 1469
Size: 792 2695
Fragments arranged by size:
2695 1737 1332 792 778 513 190 186
123 122
BsmI GAATG_Cn'
Cuts at: 2377 2921 2377
Size: 544 7924
BsmBI CGTCTCn'nnnn_
Cuts at: 905 1446 4014 4888 905
Size: 541 2568 874 4485
Fragments arranged by size:
4485 2568 874 541
Bsp1286I G_dGCh'C
Cuts at: 150 404 435 493 963 1469 1592 1778 3110
Size: 254 31 58 470 506 123 186 1332
Cuts at: 3110 4847 5360 5482 6093 6186 6260 6450 6512
Size: 1737 513 122 611 93 74 190 62
Cuts at: 6512 7242 150
Size: 730 1376
Fragments arranged by size:
1737 1376 1332 730 611 513 506 470
254 190 186 123 122 93 74 62
58 31
BspEI T'CCGG_A
Cuts at: 7688 7688
Size: 8468
BspHI T'CATG_A
Cuts at: 570 3813 4181 7644 570
Size: 3243 368 3463 1394
Fragments arranged by size:
3463 3243 1394 368
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4318 4525 5020 5518 5732 6034 6415 4318
Size: 207 495 498 214 302 381 6371
Fragments arranged by size:
6371 498 495 381 302 214 207
BsrBI CCG'CTC
Cuts at: 50 1244 3764 6760 6814 8298 8463 50
Size: 1194 2520 2996 54 1484 165 55
Fragments arranged by size:
2996 2520 1484 1194 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2597 3725 6380 2597
Size: 1128 2655 4685
Fragments arranged by size:
4685 2655 1128
BsrFI r'CCGG_y
Cuts at: 3127 6466 6647 6856 3127
Size: 3339 181 209 4739
Fragments arranged by size:
4739 3339 209 181
BsrGI T'GTAC_A
Cuts at: 2672 2672
Size: 8468
BssSI C'ACGA_G
Cuts at: 1381 1807 3963 6739 7097 1381
Size: 426 2156 2776 358 2752
Fragments arranged by size:
2776 2752 2156 426 358
BstAPI GCAn_nnn'nTGC
Cuts at: 212 2115 4327 5845 212
Size: 1903 2212 1518 2835
Fragments arranged by size:
2835 2212 1903 1518
BstBI TT'CG_AA
Cuts at: 4109 5109 6829 4109
Size: 1000 1720 5748
Fragments arranged by size:
5748 1720 1000
BstXI CCAn_nnnn'nTGG
Cuts at: 729 2766 5082 729
Size: 2037 2316 4115
Fragments arranged by size:
4115 2316 2037
BstYI r'GATC_y
Cuts at: 1 158 1622 2090 2532 3080 3154 3485 4623
Size: 157 1464 468 442 548 74 331 1138
Cuts at: 4623 5099 5493 5960 5983 6318 6564 7576 7662
Size: 476 394 467 23 335 246 1012 86
Cuts at: 7662 7674 8020 1
Size: 12 346 449
Fragments arranged by size:
1464 1138 1012 548 476 468 467 449
442 394 346 335 331 246 157 86
74 23 12
Bsu36I CC'TnA_GG
Cuts at: 1783 2465 1783
Size: 682 7786
BtgI C'CryG_G
Cuts at: 1958 3401 1958
Size: 1443 7025
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 375 6418 6599 6762 375
Size: 6043 181 163 2081
Fragments arranged by size:
6043 2081 181 163
BtsI GCAGTG_nn'
Cuts at: 266 551 647 2408 4411 5184 5711 266
Size: 285 96 1761 2003 773 527 3023
Fragments arranged by size:
3023 2003 1761 773 527 285 96
ClaI AT'CG_AT
Cuts at: 3899 3899
Size: 8468
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4387 4422 4544 4579 4387
Size: 35 122 35 8276
Fragments arranged by size:
8276 122 35 35
DraI TTT'AAA
Cuts at: 1506 1679 5467 1506
Size: 173 3788 4507
Fragments arranged by size:
4507 3788 173
DrdI GACnn_nn'nnGTC
Cuts at: 6174 7032 6174
Size: 858 7610
EaeI y'GGCC_r
Cuts at: 620 2779 3266 3752 4194 6053 6227 6618 6645
Size: 2159 487 486 442 1859 174 391 27
Cuts at: 6645 7990 620
Size: 1345 1098
Fragments arranged by size:
2159 1859 1345 1098 487 486 442 391
174 27
EagI C'GGCC_G
Cuts at: 6053 6053
Size: 8468
EarI CTCTTCn'nnn_
Cuts at: 539 1449 1496 2537 2561 3200 4180 4945 5417
Size: 910 47 1041 24 639 980 765 472
Cuts at: 5417 6491 6701 539
Size: 1074 210 2306
Fragments arranged by size:
2306 1074 1041 980 910 765 639 472
210 47 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2296 3260 6676 6986 7132 8278 2296
Size: 964 3416 310 146 1146 2486
Fragments arranged by size:
3416 2486 1146 964 310 146
EcoICRI GAG'CTC
Cuts at: 1467 1776 3108 5358 5480 1467
Size: 309 1332 2250 122 4455
Fragments arranged by size:
4455 2250 1332 309 122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 1087 1271 1430 1819 2086 2336 2818 2873
Size: 915 184 159 389 267 250 482 55
Cuts at: 2873 2964 3124 3202 4520 6293 6725 7472 7740
Size: 91 160 78 1318 1773 432 747 268
Cuts at: 7740 172
Size: 900
Fragments arranged by size:
1773 1318 915 900 747 482 432 389
268 267 250 184 160 159 91 78
55
EcoNI CCTnn'n_nnAGG
Cuts at: 1882 2155 4668 1882
Size: 273 2513 5682
Fragments arranged by size:
5682 2513 273
EcoO109I rG'GnC_Cy
Cuts at: 147 209 489 490 1282 1982 3662 4224 5607
Size: 62 280 1 792 700 1680 562 1383
Cuts at: 5607 147
Size: 3008
Fragments arranged by size:
3008 1680 1383 792 700 562 280 62
1
EcoRI G'AATT_C
Cuts at: 3066 5208 5472 3066
Size: 2142 264 6062
Fragments arranged by size:
6062 2142 264
EcoRV GAT'ATC
Cuts at: 3464 4884 3464
Size: 1420 7048
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 306 999 1245 3798 4396 6487 306
Size: 693 246 2553 598 2091 2287
Fragments arranged by size:
2553 2287 2091 693 598 246
FspI TGC'GCA
Cuts at: 205 6249 205
Size: 6044 2424
HaeII r_GCGC'y
Cuts at: 226 1704 2169 5902 6150 7172 8151 226
Size: 1478 465 3733 248 1022 979 543
Fragments arranged by size:
3733 1478 1022 979 543 465 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1093 1125 1126 1158 1282 1314 2876 2908 3510
Size: 32 1 32 124 32 1562 32 602
Cuts at: 3510 3542 4067 4099 4151 4183 4264 4296 4621
Size: 32 525 32 52 32 81 32 325
Cuts at: 4621 4653 4887 4919 6310 6340 6342 6372 1093
Size: 32 234 32 1391 30 2 30 3189
Fragments arranged by size:
3189 1562 1391 602 525 325 234 124
81 52 32 32 32 32 32 32
32 32 32 32 30 30 2 1
HincII GTy'rAC
Cuts at: 1662 3372 3582 5507 1662
Size: 1710 210 1925 4623
Fragments arranged by size:
4623 1925 1710 210
HindIII A'AGCT_T
Cuts at: 4361 4361
Size: 8468
KasI G'GCGC_C
Cuts at: 5898 6146 5898
Size: 248 8220
KpnI G_GTAC'C
Cuts at: 5440 5488 5440
Size: 48 8420
MfeI C'AATT_G
Cuts at: 4399 4399
Size: 8468
MluI A'CGCG_T
Cuts at: 7901 7901
Size: 8468
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3513 3751 4511 4757 4931 7139 7323 3513
Size: 238 760 246 174 2208 184 4658
Fragments arranged by size:
4658 2208 760 246 238 184 174
MscI TGG'CCA
Cuts at: 622 3268 4196 6229 622
Size: 2646 928 2033 2861
Fragments arranged by size:
2861 2646 2033 928
MslI CAynn'nnrTG
Cuts at: 136 694 2854 2992 3047 3967 4168 4616 5203
Size: 558 2160 138 55 920 201 448 587
Cuts at: 5203 5457 6457 6584 136
Size: 254 1000 127 2020
Fragments arranged by size:
2160 2020 1000 920 587 558 448 254
201 138 127 55
MspA1I CmG'CkG
Cuts at: 201 1021 1335 2661 2922 3102 3117 3383 3722
Size: 820 314 1326 261 180 15 266 339
Cuts at: 3722 4709 5010 5579 5893 6253 7266 7511 201
Size: 987 301 569 314 360 1013 245 1158
Fragments arranged by size:
1326 1158 1013 987 820 569 360 339
314 314 301 266 261 245 180 15
NaeI GCC'GGC
Cuts at: 6649 6649
Size: 8468
NarI GG'CG_CC
Cuts at: 5899 6147 5899
Size: 248 8220
NcoI C'CATG_G
Cuts at: 1958 3401 1958
Size: 1443 7025
NgoMIV G'CCGG_C
Cuts at: 6647 6647
Size: 8468
NheI G'CTAG_C
Cuts at: 327 327
Size: 8468
NsiI A_TGCA'T
Cuts at: 3094 3094
Size: 8468
NspI r_CATG'y
Cuts at: 135 605 874 2118 3438 3517 4561 4914 4926
Size: 470 269 1244 1320 79 1044 353 12
Cuts at: 4926 5302 5521 6021 6928 135
Size: 376 219 500 907 1675
Fragments arranged by size:
1675 1320 1244 1044 907 500 470 376
353 269 219 79 12
PasI CC'CwG_GG
Cuts at: 436 436
Size: 8468
PciI A'CATG_T
Cuts at: 601 4557 4922 6924 601
Size: 3956 365 2002 2145
Fragments arranged by size:
3956 2145 2002 365
PflMI CCAn_nnn'nTGG
Cuts at: 969 3195 969
Size: 2226 6242
PfoI T'CCnGG_A
Cuts at: 2102 3704 2102
Size: 1602 6866
PmeI GTTT'AAAC
Cuts at: 5467 5467
Size: 8468
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7607 7639 7607
Size: 32 8436
PpuMI rG'GwC_Cy
Cuts at: 1982 4224 1982
Size: 2242 6226
PspOMI G'GGCC_C
Cuts at: 146 489 146
Size: 343 8125
PstI C_TGCA'G
Cuts at: 546 590 780 1220 1334 1772 3894 5515 6200
Size: 44 190 440 114 438 2122 1621 685
Cuts at: 6200 546
Size: 2814
Fragments arranged by size:
2814 2122 1621 685 440 438 190 114
44
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8468
PvuII CAG'CTG
Cuts at: 201 1335 2922 3102 3117 4709 5893 6253 201
Size: 1134 1587 180 15 1592 1184 360 2416
Fragments arranged by size:
2416 1592 1587 1184 1134 360 180 15
RsrII CG'GwC_CG
Cuts at: 6663 6663
Size: 8468
SacI G_AGCT'C
Cuts at: 1469 1778 3110 5360 5482 1469
Size: 309 1332 2250 122 4455
Fragments arranged by size:
4455 2250 1332 309 122
SalI G'TCGA_C
Cuts at: 5505 5505
Size: 8468
SanDI GG'GwC_CC
Cuts at: 1982 1982
Size: 8468
SapI GCTCTTCn'nnn_
Cuts at: 539 4180 5417 6491 6701 539
Size: 3641 1237 1074 210 2306
Fragments arranged by size:
3641 2306 1237 1074 210
SbfI CC_TGCA'GG
Cuts at: 5515 5515
Size: 8468
ScaI AGT'ACT
Cuts at: 7985 7985
Size: 8468
SfcI C'TryA_G
Cuts at: 33 542 586 776 1216 1330 1768 2447 2820
Size: 509 44 190 440 114 438 679 373
Cuts at: 2820 3493 3890 4580 5511 6196 7189 7380 33
Size: 673 397 690 931 685 993 191 1121
Fragments arranged by size:
1121 993 931 690 685 679 673 509
440 438 397 373 191 190 114 44
SfoI GGC'GCC
Cuts at: 5900 6148 5900
Size: 248 8220
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8468
SmaI CCC'GGG
Cuts at: 145 5490 145
Size: 5345 3123
SmlI C'TyrA_G
Cuts at: 1819 2553 3613 5623 6538 7030 7292 1819
Size: 734 1060 2010 915 492 262 2995
Fragments arranged by size:
2995 2010 1060 915 734 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8468
SphI G_CATG'C
Cuts at: 2118 5521 6021 2118
Size: 3403 500 4565
Fragments arranged by size:
4565 3403 500
StuI AGG'CCT
Cuts at: 1796 1925 4020 4987 5230 1796
Size: 129 2095 967 243 5034
Fragments arranged by size:
5034 2095 967 243 129
StyI C'CwwG_G
Cuts at: 349 653 1272 1316 1958 2379 3060 3219 3401
Size: 304 619 44 642 421 681 159 182
Cuts at: 3401 5602 5956 349
Size: 2201 354 2861
Fragments arranged by size:
2861 2201 681 642 619 421 354 304
182 159 44
SwaI ATTT'AAAT
Cuts at: 1679 1679
Size: 8468
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 933 1870 2194 2351 6051 6184 6643 6852 933
Size: 937 324 157 3700 133 459 209 2549
Fragments arranged by size:
3700 2549 937 459 324 209 157 133
TatI w'GTAC_w
Cuts at: 2672 4919 5309 7983 2672
Size: 2247 390 2674 3157
Fragments arranged by size:
3157 2674 2247 390
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1175 1207 3116 3148 7985 8017 1175
Size: 32 1909 32 4837 32 1626
Fragments arranged by size:
4837 1909 1626 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2773 3376 4075 6265 2773
Size: 603 699 2190 4976
Fragments arranged by size:
4976 2190 699 603
XbaI T'CTAG_A
Cuts at: 5499 5499
Size: 8468
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2715 3283 3408 3708 5963 2715
Size: 568 125 300 2255 5220
Fragments arranged by size:
5220 2255 568 300 125
XmaI C'CCGG_G
Cuts at: 143 5488 143
Size: 5345 3123
XmnI GAAnn'nnTTC
Cuts at: 509 3977 4376 5380 509
Size: 3468 399 1004 3597
Fragments arranged by size:
3597 3468 1004 399
ZraI GAC'GTC
Cuts at: 1955 1955
Size: 8468
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AloI AscI AvrII BaeI BbvCI
BmgBI BsiWI BssHII BstEII BstZ17I DraIII FseI FspAI
HpaI NdeI NotI NruI PacI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI XhoI