Restriction map for
pF1KB4366
(Circular) (Six-base) MAPSORT of: pF1KB4366.seq Check: 7505 from: 1 to: 5882
pF1KB4366 5882 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1767 1889 1767
Size: 122 5760
AccI GT'mk_AC
Cuts at: 2920 2920
Size: 5882
Acc65I G'GTAC_C
Cuts at: 1993 2898 1993
Size: 905 4977
AclI AA'CG_TT
Cuts at: 5722 5722
Size: 5882
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 2684 3707 4139 4886 5154 293
Size: 2391 1023 432 747 268 1021
Fragments arranged by size:
2391 1023 1021 747 432 268
AflIII A'CryG_T
Cuts at: 1759 3234 4338 5315 1759
Size: 1475 1104 977 2326
Fragments arranged by size:
2326 1475 1104 977
AgeI A'CCGG_T
Cuts at: 4270 4270
Size: 5882
AleI CACnn'nnGTG
Cuts at: 1873 1873
Size: 5882
AlwNI CAG_nnn'CTG
Cuts at: 3187 4754 3187
Size: 1567 4315
ApaLI G'TGCA_C
Cuts at: 2082 4652 2082
Size: 2570 3312
ApoI r'AATT_y
Cuts at: 492 1277 1303 2886 5235 5491 492
Size: 785 26 1583 2349 256 883
Fragments arranged by size:
2349 1583 883 785 256 26
AseI AT'TA_AT
Cuts at: 20 544 20
Size: 524 5358
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5882
AvaI C'yCGr_G
Cuts at: 329 2151 2902 329
Size: 1822 751 3309
Fragments arranged by size:
3309 1822 751
BamHI G'GATC_C
Cuts at: 2907 2907
Size: 5882
BanI G'GyrC_C
Cuts at: 1993 2898 3312 3560 3595 1993
Size: 905 414 248 35 4280
Fragments arranged by size:
4280 905 414 248 35
BanII G_rGCy'C
Cuts at: 1400 2896 3926 1400
Size: 1496 1030 3356
Fragments arranged by size:
3356 1496 1030
BbeI G_GCGC'C
Cuts at: 3316 3564 3316
Size: 248 5634
BbsI GAAGACnn'nnnn_
Cuts at: 2744 2744
Size: 5882
BciVI GTATCCnnnnn_n'
Cuts at: 2396 2559 3780 4541 5882 2396
Size: 163 1221 761 1341 2396
Fragments arranged by size:
2396 1341 1221 761 163
BclI T'GATC_A
Cuts at: 1581 3402 1581
Size: 1821 4061
BglII A'GATC_T
Cuts at: 1929 3397 5434 1929
Size: 1468 2037 2377
Fragments arranged by size:
2377 2037 1468
BlpI GC'TnA_GC
Cuts at: 2994 2994
Size: 5882
Bme1580I G_kGCm'C
Cuts at: 2086 2430 3507 3600 4656 2086
Size: 344 1077 93 1056 3312
Fragments arranged by size:
3312 1077 1056 344 93
BmgBI CAC'GTC
Cuts at: 960 960
Size: 5882
BmrI ACTGGGnnnn_n'
Cuts at: 2184 2405 3094 3175 3510 5654 2184
Size: 221 689 81 335 2144 2412
Fragments arranged by size:
2412 2144 689 335 221 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4249 4249
Size: 5882
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 1266 1370 1529 2722 2866 736
Size: 530 104 159 1193 144 3752
Fragments arranged by size:
3752 1193 530 159 144 104
Bpu10I CC'TnA_GC
Cuts at: 1766 1766
Size: 5882
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 291 3058 3937 4429 4727 208
Size: 83 2767 879 492 298 1363
Fragments arranged by size:
2767 1363 879 492 298 83
BsaI GGTCTCn'nnnn_
Cuts at: 2768 5586 2768
Size: 2818 3064
BsaAI yAC'GTr
Cuts at: 3235 3235
Size: 5882
BsaBI GATnn'nnATC
Cuts at: 6 1547 1553 1856 3396 5433 6
Size: 1541 6 303 1540 2037 455
Fragments arranged by size:
2037 1541 1540 455 303 6
BsaHI Gr'CG_yC
Cuts at: 258 3313 3561 4263 5759 258
Size: 3055 248 702 1496 381
Fragments arranged by size:
3055 1496 702 381 248
BsaWI w'CCGG_w
Cuts at: 3071 3592 4270 4544 4691 5102 3071
Size: 521 678 274 147 411 3851
Fragments arranged by size:
3851 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2619 2649 2619
Size: 30 5852
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 841 1526 2764 2767 841
Size: 685 1238 3 3956
Fragments arranged by size:
3956 1238 685 3
BseYI C'CCAG_C
Cuts at: 3307 4065 4642 5138 3307
Size: 758 577 496 4051
Fragments arranged by size:
4051 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1330 1868 1330
Size: 538 5344
BsiEI CG_ry'CG
Cuts at: 104 633 776 2215 3424 3470 4678 104
Size: 529 143 1439 1209 46 1208 1308
Fragments arranged by size:
1439 1308 1209 1208 529 143 46
BsiHKAI G_wGCw'C
Cuts at: 1767 2086 2896 3674 3864 4656 1767
Size: 319 810 778 190 792 2993
Fragments arranged by size:
2993 810 792 778 319 190
BsmI GAATG_Cn'
Cuts at: 1321 1321
Size: 5882
BsmBI CGTCTCn'nnnn_
Cuts at: 2595 2595
Size: 5882
Bsp1286I G_dGCh'C
Cuts at: 1400 1767 2086 2430 2896 3507 3600 3674 3864
Size: 367 319 344 466 611 93 74 190
Cuts at: 3864 3926 4656 1400
Size: 62 730 2626
Fragments arranged by size:
2626 730 611 466 367 344 319 190
93 74 62
BspEI T'CCGG_A
Cuts at: 5102 5102
Size: 5882
BspHI T'CATG_A
Cuts at: 1455 1545 2016 5058 1455
Size: 90 471 3042 2279
Fragments arranged by size:
3042 2279 471 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1767 1889 2932 3146 3448 3829 1767
Size: 122 1043 214 302 381 3820
Fragments arranged by size:
3820 1043 381 302 214 122
BsrBI CCG'CTC
Cuts at: 50 1250 4174 4228 5712 5877 50
Size: 1200 2924 54 1484 165 55
Fragments arranged by size:
2924 1484 1200 165 55 54
BsrDI GCAATG_nn'
Cuts at: 794 3794 794
Size: 3000 2882
BsrFI r'CCGG_y
Cuts at: 121 3880 4061 4270 121
Size: 3759 181 209 1733
Fragments arranged by size:
3759 1733 209 181
BssSI C'ACGA_G
Cuts at: 194 4153 4511 194
Size: 3959 358 1565
Fragments arranged by size:
3959 1565 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3259 3259
Size: 5882
BstBI TT'CG_AA
Cuts at: 4243 4243
Size: 5882
BstXI CCAn_nnnn'nTGG
Cuts at: 2700 2844 2700
Size: 144 5738
BstYI r'GATC_y
Cuts at: 1 1929 2011 2290 2907 3374 3397 3732 3978
Size: 1928 82 279 617 467 23 335 246
Cuts at: 3978 4990 5076 5088 5434 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1928 1012 617 467 449 346 335 279
246 86 82 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5882
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3832 4013 4176 3832
Size: 181 163 5538
Fragments arranged by size:
5538 181 163
BtsI GCAGTG_nn'
Cuts at: 991 1318 1470 3125 991
Size: 327 152 1655 3748
Fragments arranged by size:
3748 1655 327 152
ClaI AT'CG_AT
Cuts at: 2211 2211
Size: 5882
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 348 383 348
Size: 35 5847
DraI TTT'AAA
Cuts at: 2881 2881
Size: 5882
DrdI GACnn_nn'nnGTC
Cuts at: 3588 4446 3588
Size: 858 5024
EaeI y'GGCC_r
Cuts at: 634 1877 2281 3467 3641 4032 4059 5404 634
Size: 1243 404 1186 174 391 27 1345 1112
Fragments arranged by size:
1345 1243 1186 1112 404 391 174 27
EagI C'GGCC_G
Cuts at: 3467 3467
Size: 5882
EarI CTCTTCn'nnn_
Cuts at: 2598 2660 2756 2792 3905 4115 2598
Size: 62 96 36 1113 210 4365
Fragments arranged by size:
4365 1113 210 96 62 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4090 4400 4546 5692 4090
Size: 310 146 1146 4280
Fragments arranged by size:
4280 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2894 2894
Size: 5882
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 736 1266 1370 1529 2684 2722 2866 3707
Size: 443 530 104 159 1155 38 144 841
Cuts at: 3707 4139 4886 5154 293
Size: 432 747 268 1021
Fragments arranged by size:
1155 1021 841 747 530 443 432 268
159 144 104 38
EcoNI CCTnn'n_nnAGG
Cuts at: 1409 2845 1409
Size: 1436 4446
EcoO109I rG'GnC_Cy
Cuts at: 1414 3021 1414
Size: 1607 4275
EcoRI G'AATT_C
Cuts at: 2886 2886
Size: 5882
EcoRV GAT'ATC
Cuts at: 2307 2483 2307
Size: 176 5706
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 442 2857 3901 442
Size: 2415 1044 2423
Fragments arranged by size:
2423 2415 1044
FspI TGC'GCA
Cuts at: 3663 3663
Size: 5882
HaeII r_GCGC'y
Cuts at: 3316 3564 4586 5565 3316
Size: 248 1022 979 3633
Fragments arranged by size:
3633 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 1749 1781 3724 3754 3756 3786 163
Size: 32 1554 32 1943 30 2 30 2259
Fragments arranged by size:
2259 1943 1554 32 32 30 30 2
HincII GTy'rAC
Cuts at: 666 2921 666
Size: 2255 3627
KasI G'GCGC_C
Cuts at: 3312 3560 3312
Size: 248 5634
KpnI G_GTAC'C
Cuts at: 1997 2902 1997
Size: 905 4977
MfeI C'AATT_G
Cuts at: 407 407
Size: 5882
MluI A'CGCG_T
Cuts at: 5315 5315
Size: 5882
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 796 4553 4737 796
Size: 3757 184 1941
Fragments arranged by size:
3757 1941 184
MscI TGG'CCA
Cuts at: 1879 2283 3643 1879
Size: 404 1360 4118
Fragments arranged by size:
4118 1360 404
MslI CAynn'nnrTG
Cuts at: 395 581 1073 1709 1862 1873 3871 3998 395
Size: 186 492 636 153 11 1998 127 2279
Fragments arranged by size:
2279 1998 636 492 186 153 127 11
MspA1I CmG'CkG
Cuts at: 466 2662 2771 2993 3307 3667 4680 4925 466
Size: 2196 109 222 314 360 1013 245 1423
Fragments arranged by size:
2196 1423 1013 360 314 245 222 109
NaeI GCC'GGC
Cuts at: 123 4063 123
Size: 3940 1942
NarI GG'CG_CC
Cuts at: 3313 3561 3313
Size: 248 5634
NcoI C'CATG_G
Cuts at: 107 107
Size: 5882
NdeI CA'TA_TG
Cuts at: 726 1100 726
Size: 374 5508
NgoMIV G'CCGG_C
Cuts at: 121 4061 121
Size: 3940 1942
NspI r_CATG'y
Cuts at: 1217 1763 2935 3435 4342 1217
Size: 546 1172 500 907 2757
Fragments arranged by size:
2757 1172 907 546 500
PciI A'CATG_T
Cuts at: 1759 4338 1759
Size: 2579 3303
PmeI GTTT'AAAC
Cuts at: 2881 2881
Size: 5882
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5021 5053 5021
Size: 32 5850
PpuMI rG'GwC_Cy
Cuts at: 1414 1414
Size: 5882
PspXI vC'TCGA_Gb
Cuts at: 329 329
Size: 5882
PstI C_TGCA'G
Cuts at: 570 998 2929 3614 570
Size: 428 1931 685 2838
Fragments arranged by size:
2838 1931 685 428
PvuI CG_AT'CG
Cuts at: 104 2215 104
Size: 2111 3771
PvuII CAG'CTG
Cuts at: 466 2662 3307 3667 466
Size: 2196 645 360 2681
Fragments arranged by size:
2681 2196 645 360
RsrII CG'GwC_CG
Cuts at: 4077 4077
Size: 5882
SacI G_AGCT'C
Cuts at: 2896 2896
Size: 5882
SalI G'TCGA_C
Cuts at: 2919 2919
Size: 5882
SapI GCTCTTCn'nnn_
Cuts at: 2792 3905 4115 2792
Size: 1113 210 4559
Fragments arranged by size:
4559 1113 210
SbfI CC_TGCA'GG
Cuts at: 2929 2929
Size: 5882
ScaI AGT'ACT
Cuts at: 156 812 5399 156
Size: 656 4587 639
Fragments arranged by size:
4587 656 639
SfcI C'TryA_G
Cuts at: 33 566 747 994 1391 1604 2006 2558 2925
Size: 533 181 247 397 213 402 552 367
Cuts at: 2925 3610 4603 4794 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 552 533 402 397 367
247 213 191 181
SfoI GGC'GCC
Cuts at: 3314 3562 3314
Size: 248 5634
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5882
SmaI CCC'GGG
Cuts at: 2904 2904
Size: 5882
SmlI C'TyrA_G
Cuts at: 187 306 329 3037 3952 4444 4706 187
Size: 119 23 2708 915 492 262 1363
Fragments arranged by size:
2708 1363 915 492 262 119 23
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5882
SphI G_CATG'C
Cuts at: 2935 3435 2935
Size: 500 5382
SspI AAT'ATT
Cuts at: 2168 2168
Size: 5882
StuI AGG'CCT
Cuts at: 945 945
Size: 5882
StyI C'CwwG_G
Cuts at: 107 2778 3016 3370 107
Size: 2671 238 354 2619
Fragments arranged by size:
2671 2619 354 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 552 1202 3465 3598 4057 4266 552
Size: 650 2263 133 459 209 2168
Fragments arranged by size:
2263 2168 650 459 209 133
TatI w'GTAC_w
Cuts at: 154 810 1695 2171 2420 5397 154
Size: 656 885 476 249 2977 639
Fragments arranged by size:
2977 885 656 639 476 249
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2619 2651 5399 5431 2619
Size: 32 2748 32 3070
Fragments arranged by size:
3070 2748 32 32
Tth111I GACn'n_nGTC
Cuts at: 3679 3679
Size: 5882
XbaI T'CTAG_A
Cuts at: 2913 2913
Size: 5882
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3377 3377
Size: 5882
XhoI C'TCGA_G
Cuts at: 329 329
Size: 5882
XmaI C'CCGG_G
Cuts at: 2902 2902
Size: 5882
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PmeI PpiI PpuMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BsiWI
BsrGI BssHII BstEII BstZ17I Bsu36I DraIII FseI FspAI
HindIII HpaI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XmnI
ZraI