Restriction map for
pF1KB4343
(Circular) (Six-base) MAPSORT of: pF1KB4343.seq Check: 8675 from: 1 to: 4205
pF1KB4343 4205 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 707 1243 707
Size: 536 3669
Acc65I G'GTAC_C
Cuts at: 1221 1221
Size: 4205
AclI AA'CG_TT
Cuts at: 4045 4045
Size: 4205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 548 815 2030 2462 2815 3477 548
Size: 267 1215 432 353 662 1276
Fragments arranged by size:
1276 1215 662 432 353 267
AflIII A'CryG_T
Cuts at: 1557 3357 3638 1557
Size: 1800 281 2124
Fragments arranged by size:
2124 1800 281
AleI CACnn'nnGTG
Cuts at: 852 852
Size: 4205
AlwNI CAG_nnn'CTG
Cuts at: 132 1510 2948 132
Size: 1378 1438 1389
Fragments arranged by size:
1438 1389 1378
ApaI G_GGCC'C
Cuts at: 117 117
Size: 4205
ApaLI G'TGCA_C
Cuts at: 767 1107 3043 767
Size: 340 1936 1929
Fragments arranged by size:
1936 1929 340
ApoI r'AATT_y
Cuts at: 713 1163 1209 3558 3814 713
Size: 450 46 2349 256 1104
Fragments arranged by size:
2349 1104 450 256 46
AseI AT'TA_AT
Cuts at: 20 20
Size: 4205
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
AvaI C'yCGr_G
Cuts at: 1225 1225
Size: 4205
BamHI G'GATC_C
Cuts at: 1230 1230
Size: 4205
BanI G'GyrC_C
Cuts at: 1221 1635 1883 1918 1221
Size: 414 248 35 3508
Fragments arranged by size:
3508 414 248 35
BanII G_rGCy'C
Cuts at: 117 1148 1219 2249 117
Size: 1031 71 1030 2073
Fragments arranged by size:
2073 1031 1030 71
BbeI G_GCGC'C
Cuts at: 1639 1887 1639
Size: 248 3957
BbvCI CC'TCA_GC
Cuts at: 397 397
Size: 4205
BciVI GTATCCnnnnn_n'
Cuts at: 2103 3159 4205 2103
Size: 1056 1046 2103
Fragments arranged by size:
2103 1056 1046
BclI T'GATC_A
Cuts at: 1725 1725
Size: 4205
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4205
BglII A'GATC_T
Cuts at: 1720 3757 1720
Size: 2037 2168
BlpI GC'TnA_GC
Cuts at: 1317 1317
Size: 4205
Bme1580I G_kGCm'C
Cuts at: 117 771 1111 1830 1923 3047 117
Size: 654 340 719 93 1124 1275
Fragments arranged by size:
1275 1124 719 654 340 93
BmrI ACTGGGnnnn_n'
Cuts at: 142 1417 1498 1833 3977 142
Size: 1275 81 335 2144 370
Fragments arranged by size:
2144 1275 370 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4205
Bpu10I CC'TnA_GC
Cuts at: 397 397
Size: 4205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 1381 2260 2974 3272 289
Size: 1092 879 714 298 1222
Fragments arranged by size:
1222 1092 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 327 618 3909 327
Size: 291 3291 623
Fragments arranged by size:
3291 623 291
BsaAI yAC'GTr
Cuts at: 1558 1558
Size: 4205
BsaBI GATnn'nnATC
Cuts at: 6 512 1719 3756 6
Size: 506 1207 2037 455
Fragments arranged by size:
2037 1207 506 455
BsaHI Gr'CG_yC
Cuts at: 557 1636 1884 2586 4082 557
Size: 1079 248 702 1496 680
Fragments arranged by size:
1496 1079 702 680 248
BsaWI w'CCGG_w
Cuts at: 1394 1915 2593 3004 3151 3425 1394
Size: 521 678 411 147 274 2174
Fragments arranged by size:
2174 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1084 1114 1084
Size: 30 4175
BseYI C'CCAG_C
Cuts at: 1630 2388 3053 3461 1630
Size: 758 665 408 2374
Fragments arranged by size:
2374 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 876 1044 1212 876
Size: 168 168 3869
Fragments arranged by size:
3869 168 168
BsiEI CG_ry'CG
Cuts at: 104 1747 1793 3023 104
Size: 1643 46 1230 1286
Fragments arranged by size:
1643 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 771 1111 1148 1219 1997 2187 3047 771
Size: 340 37 71 778 190 860 1929
Fragments arranged by size:
1929 860 778 340 190 71 37
BsmI GAATG_Cn'
Cuts at: 693 693
Size: 4205
Bsp1286I G_dGCh'C
Cuts at: 117 771 1111 1148 1219 1830 1923 1997 2187
Size: 654 340 37 71 611 93 74 190
Cuts at: 2187 2249 3047 117
Size: 62 798 1275
Fragments arranged by size:
1275 798 654 611 340 190 93 74
71 62 37
BspHI T'CATG_A
Cuts at: 2637 2637
Size: 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 364 1255 1469 1771 2152 364
Size: 891 214 302 381 2417
Fragments arranged by size:
2417 891 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2497 2551 4035 4200 50
Size: 2447 54 1484 165 55
Fragments arranged by size:
2447 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2117 2117
Size: 4205
BsrFI r'CCGG_y
Cuts at: 2203 2384 2203
Size: 181 4024
BssSI C'ACGA_G
Cuts at: 2476 3184 2476
Size: 708 3497
BstAPI GCAn_nnn'nTGC
Cuts at: 1582 1582
Size: 4205
BstBI TT'CG_AA
Cuts at: 2566 2566
Size: 4205
BstYI r'GATC_y
Cuts at: 1 1230 1697 1720 2055 2301 2607 2619 2705
Size: 1229 467 23 335 246 306 12 86
Cuts at: 2705 3757 1
Size: 1052 449
Fragments arranged by size:
1229 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 118 118
Size: 4205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2155 2336 2499 2155
Size: 181 163 3861
Fragments arranged by size:
3861 181 163
BtsI GCAGTG_nn'
Cuts at: 1448 1448
Size: 4205
DraI TTT'AAA
Cuts at: 1204 1204
Size: 4205
DraIII CAC_nnn'GTG
Cuts at: 1115 1115
Size: 4205
DrdI GACnn_nn'nnGTC
Cuts at: 1911 3255 1911
Size: 1344 2861
EaeI y'GGCC_r
Cuts at: 1790 1964 2355 2382 3727 1790
Size: 174 391 27 1345 2268
Fragments arranged by size:
2268 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1790 1790
Size: 4205
EarI CTCTTCn'nnn_
Cuts at: 179 250 2228 2438 179
Size: 71 1978 210 1946
Fragments arranged by size:
1978 1946 210 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2413 3155 3301 4015 2413
Size: 742 146 714 2603
Fragments arranged by size:
2603 742 714 146
EcoICRI GAG'CTC
Cuts at: 1146 1217 1146
Size: 71 4134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 548 815 2030 2462 2815 3477 548
Size: 267 1215 432 353 662 1276
Fragments arranged by size:
1276 1215 662 432 353 267
EcoNI CCTnn'n_nnAGG
Cuts at: 428 428
Size: 4205
EcoO109I rG'GnC_Cy
Cuts at: 114 1344 114
Size: 1230 2975
EcoRI G'AATT_C
Cuts at: 1163 1209 1163
Size: 46 4159
EcoRV GAT'ATC
Cuts at: 199 199
Size: 4205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 611 2224 611
Size: 1613 2592
FspI TGC'GCA
Cuts at: 1986 1986
Size: 4205
HaeII r_GCGC'y
Cuts at: 1639 1887 3117 3888 1639
Size: 248 1230 771 1956
Fragments arranged by size:
1956 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2047 2077 2079 2109 2047
Size: 30 2 30 4143
Fragments arranged by size:
4143 30 30 2
HincII GTy'rAC
Cuts at: 1244 1244
Size: 4205
KasI G'GCGC_C
Cuts at: 1635 1883 1635
Size: 248 3957
KpnI G_GTAC'C
Cuts at: 1225 1225
Size: 4205
MluI A'CGCG_T
Cuts at: 3638 3638
Size: 4205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2964 3148 2964
Size: 184 4021
MscI TGG'CCA
Cuts at: 1966 1966
Size: 4205
MslI CAynn'nnrTG
Cuts at: 852 2194 2321 852
Size: 1342 127 2736
Fragments arranged by size:
2736 1342 127
MspA1I CmG'CkG
Cuts at: 120 1316 1630 1990 2774 3019 120
Size: 1196 314 360 784 245 1306
Fragments arranged by size:
1306 1196 784 360 314 245
NaeI GCC'GGC
Cuts at: 2386 2386
Size: 4205
NarI GG'CG_CC
Cuts at: 1636 1884 1636
Size: 248 3957
NgoMIV G'CCGG_C
Cuts at: 2384 2384
Size: 4205
NspI r_CATG'y
Cuts at: 1258 1758 3361 1258
Size: 500 1603 2102
Fragments arranged by size:
2102 1603 500
PasI CC'CwG_GG
Cuts at: 179 736 179
Size: 557 3648
PciI A'CATG_T
Cuts at: 3357 3357
Size: 4205
PflMI CCAn_nnn'nTGG
Cuts at: 742 742
Size: 4205
PmeI GTTT'AAAC
Cuts at: 1204 1204
Size: 4205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2651 2683 2651
Size: 32 4173
PspOMI G'GGCC_C
Cuts at: 113 113
Size: 4205
PstI C_TGCA'G
Cuts at: 1252 1937 1252
Size: 685 3520
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4205
PvuII CAG'CTG
Cuts at: 1630 1990 1630
Size: 360 3845
RsrII CG'GwC_CG
Cuts at: 2400 2400
Size: 4205
SacI G_AGCT'C
Cuts at: 1148 1219 1148
Size: 71 4134
SacII CC_GC'GG
Cuts at: 121 121
Size: 4205
SalI G'TCGA_C
Cuts at: 1242 1242
Size: 4205
SapI GCTCTTCn'nnn_
Cuts at: 2228 2438 2228
Size: 210 3995
SbfI CC_TGCA'GG
Cuts at: 1252 1252
Size: 4205
ScaI AGT'ACT
Cuts at: 3722 3722
Size: 4205
SfcI C'TryA_G
Cuts at: 33 1248 1933 2901 3092 33
Size: 1215 685 968 191 1146
Fragments arranged by size:
1215 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1637 1885 1637
Size: 248 3957
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
SmaI CCC'GGG
Cuts at: 1227 1227
Size: 4205
SmlI C'TyrA_G
Cuts at: 268 1360 2275 2989 3251 268
Size: 1092 915 714 262 1222
Fragments arranged by size:
1222 1092 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4205
SphI G_CATG'C
Cuts at: 1258 1758 1258
Size: 500 3705
SspI AAT'ATT
Cuts at: 659 659
Size: 4205
StyI C'CwwG_G
Cuts at: 161 1339 1693 161
Size: 1178 354 2673
Fragments arranged by size:
2673 1178 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 473 1788 1921 2380 2589 473
Size: 1315 133 459 209 2089
Fragments arranged by size:
2089 1315 459 209 133
TatI w'GTAC_w
Cuts at: 577 994 3720 577
Size: 417 2726 1062
Fragments arranged by size:
2726 1062 417
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3722 3754 3722
Size: 32 4173
Tth111I GACn'n_nGTC
Cuts at: 2002 2002
Size: 4205
XbaI T'CTAG_A
Cuts at: 1236 1236
Size: 4205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 638 1700 638
Size: 1062 3143
XmaI C'CCGG_G
Cuts at: 1225 1225
Size: 4205
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BpmI BseRI BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI