Restriction map for
pF1KB4334
(Circular) (Six-base) MAPSORT of: pF1KB4334.seq Check: 7410 from: 1 to: 4532
pF1KB4334 4532 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 1570 1570
Size: 4532
Acc65I G'GTAC_C
Cuts at: 1548 1548
Size: 4532
AclI AA'CG_TT
Cuts at: 1526 4372 1526
Size: 2846 1686
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 2357 2789 3536 3804 835
Size: 1522 432 747 268 1563
Fragments arranged by size:
1563 1522 747 432 268
AflIII A'CryG_T
Cuts at: 206 1028 1884 2988 3965 206
Size: 822 856 1104 977 773
Fragments arranged by size:
1104 977 856 822 773
AgeI A'CCGG_T
Cuts at: 2920 2920
Size: 4532
AhdI GACnn_n'nnGTC
Cuts at: 489 1458 489
Size: 969 3563
AleI CACnn'nnGTG
Cuts at: 952 952
Size: 4532
AlwNI CAG_nnn'CTG
Cuts at: 1837 3404 1837
Size: 1567 2965
ApaLI G'TGCA_C
Cuts at: 3302 3302
Size: 4532
ApoI r'AATT_y
Cuts at: 1275 1536 3885 4141 1275
Size: 261 2349 256 1666
Fragments arranged by size:
2349 1666 261 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4532
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4532
AvaI C'yCGr_G
Cuts at: 199 765 940 1331 1552 199
Size: 566 175 391 221 3179
Fragments arranged by size:
3179 566 391 221 175
BamHI G'GATC_C
Cuts at: 410 1557 410
Size: 1147 3385
BanI G'GyrC_C
Cuts at: 459 790 1548 1962 2210 2245 459
Size: 331 758 414 248 35 2746
Fragments arranged by size:
2746 758 414 331 248 35
BanII G_rGCy'C
Cuts at: 566 731 1546 2576 566
Size: 165 815 1030 2522
Fragments arranged by size:
2522 1030 815 165
BbeI G_GCGC'C
Cuts at: 1966 2214 1966
Size: 248 4284
BbsI GAAGACnn'nnnn_
Cuts at: 526 771 1387 1394 526
Size: 245 616 7 3664
Fragments arranged by size:
3664 616 245 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1426 1460 1426
Size: 34 4498
BciVI GTATCCnnnnn_n'
Cuts at: 832 2430 3191 4532 832
Size: 1598 761 1341 832
Fragments arranged by size:
1598 1341 832 761
BclI T'GATC_A
Cuts at: 2052 2052
Size: 4532
BfrBI ATG'CAT
Cuts at: 590 919 590
Size: 329 4203
BglII A'GATC_T
Cuts at: 2047 4084 2047
Size: 2037 2495
BlpI GC'TnA_GC
Cuts at: 1644 1644
Size: 4532
Bme1580I G_kGCm'C
Cuts at: 464 2157 2250 3306 464
Size: 1693 93 1056 1690
Fragments arranged by size:
1693 1690 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1744 1825 2160 4304 1744
Size: 81 335 2144 1972
Fragments arranged by size:
2144 1972 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 955 1346 2899 955
Size: 391 1553 2588
Fragments arranged by size:
2588 1553 391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1093 1093
Size: 4532
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1708 2587 3079 3377 1708
Size: 879 492 298 2863
Fragments arranged by size:
2863 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 768 985 1254 4236 768
Size: 217 269 2982 1064
Fragments arranged by size:
2982 1064 269 217
BsaAI yAC'GTr
Cuts at: 1885 1885
Size: 4532
BsaBI GATnn'nnATC
Cuts at: 6 304 2046 4083 6
Size: 298 1742 2037 455
Fragments arranged by size:
2037 1742 455 298
BsaHI Gr'CG_yC
Cuts at: 1963 2211 2913 4409 1963
Size: 248 702 1496 2086
Fragments arranged by size:
2086 1496 702 248
BsaWI w'CCGG_w
Cuts at: 709 1721 2242 2920 3194 3341 3752 709
Size: 1012 521 678 274 147 411 1489
Fragments arranged by size:
1489 1012 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 497 527 497
Size: 30 4502
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1410 1410
Size: 4532
BseYI C'CCAG_C
Cuts at: 1957 2715 3292 3788 1957
Size: 758 577 496 2701
Fragments arranged by size:
2701 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 615 615
Size: 4532
BsiEI CG_ry'CG
Cuts at: 104 2074 2120 3328 104
Size: 1970 46 1208 1308
Fragments arranged by size:
1970 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 731 755 1546 2324 2514 3306 731
Size: 24 791 778 190 792 1957
Fragments arranged by size:
1957 792 791 778 190 24
BsmI GAATG_Cn'
Cuts at: 1449 1449
Size: 4532
BsmBI CGTCTCn'nnnn_
Cuts at: 217 217
Size: 4532
Bsp1286I G_dGCh'C
Cuts at: 464 566 731 755 1546 2157 2250 2324 2514
Size: 102 165 24 791 611 93 74 190
Cuts at: 2514 2576 3306 464
Size: 62 730 1690
Fragments arranged by size:
1690 791 730 611 190 165 102 93
74 62 24
BspEI T'CCGG_A
Cuts at: 3752 3752
Size: 4532
BspHI T'CATG_A
Cuts at: 3708 3708
Size: 4532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1582 1796 2098 2479 1582
Size: 214 302 381 3635
Fragments arranged by size:
3635 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2824 2878 4362 4527 50
Size: 2774 54 1484 165 55
Fragments arranged by size:
2774 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2444 2444
Size: 4532
BsrFI r'CCGG_y
Cuts at: 2530 2711 2920 2530
Size: 181 209 4142
Fragments arranged by size:
4142 209 181
BssSI C'ACGA_G
Cuts at: 2803 3161 2803
Size: 358 4174
BstAPI GCAn_nnn'nTGC
Cuts at: 925 1909 925
Size: 984 3548
BstBI TT'CG_AA
Cuts at: 2893 2893
Size: 4532
BstYI r'GATC_y
Cuts at: 1 410 1557 2024 2047 2382 2628 3640 3726
Size: 409 1147 467 23 335 246 1012 86
Cuts at: 3726 3738 4084 1
Size: 12 346 449
Fragments arranged by size:
1147 1012 467 449 409 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 601 601
Size: 4532
BtgI C'CryG_G
Cuts at: 662 662
Size: 4532
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 297 1202 2482 2663 2826 297
Size: 905 1280 181 163 2003
Fragments arranged by size:
2003 1280 905 181 163
BtsI GCAGTG_nn'
Cuts at: 1775 1775
Size: 4532
DraI TTT'AAA
Cuts at: 1531 1531
Size: 4532
DrdI GACnn_nn'nnGTC
Cuts at: 2238 3096 2238
Size: 858 3674
EaeI y'GGCC_r
Cuts at: 2117 2291 2682 2709 4054 2117
Size: 174 391 27 1345 2595
Fragments arranged by size:
2595 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2117 2117
Size: 4532
EarI CTCTTCn'nnn_
Cuts at: 820 1101 2555 2765 820
Size: 281 1454 210 2587
Fragments arranged by size:
2587 1454 281 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2740 3050 3196 4342 2740
Size: 310 146 1146 2930
Fragments arranged by size:
2930 1146 310 146
EcoICRI GAG'CTC
Cuts at: 729 1544 729
Size: 815 3717
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 1093 2357 2789 3536 3804 835
Size: 258 1264 432 747 268 1563
Fragments arranged by size:
1563 1264 747 432 268 258
EcoNI CCTnn'n_nnAGG
Cuts at: 1334 1334
Size: 4532
EcoO109I rG'GnC_Cy
Cuts at: 114 280 1111 1671 114
Size: 166 831 560 2975
Fragments arranged by size:
2975 831 560 166
EcoRI G'AATT_C
Cuts at: 1275 1536 1275
Size: 261 4271
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 4532
FspI TGC'GCA
Cuts at: 2313 2313
Size: 4532
HaeII r_GCGC'y
Cuts at: 1966 2214 3236 4215 1966
Size: 248 1022 979 2283
Fragments arranged by size:
2283 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 475 507 1444 1476 2374 2404 2406 2436 475
Size: 32 937 32 898 30 2 30 2571
Fragments arranged by size:
2571 937 898 32 32 30 30 2
HincII GTy'rAC
Cuts at: 342 1089 1214 1524 1571 342
Size: 747 125 310 47 3303
Fragments arranged by size:
3303 747 310 125 47
HindIII A'AGCT_T
Cuts at: 1129 1129
Size: 4532
HpaI GTT'AAC
Cuts at: 1524 1524
Size: 4532
KasI G'GCGC_C
Cuts at: 1962 2210 1962
Size: 248 4284
KpnI G_GTAC'C
Cuts at: 1552 1552
Size: 4532
MluI A'CGCG_T
Cuts at: 3965 3965
Size: 4532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3203 3387 3203
Size: 184 4348
MscI TGG'CCA
Cuts at: 2293 2293
Size: 4532
MslI CAynn'nnrTG
Cuts at: 325 542 952 1027 2521 2648 325
Size: 217 410 75 1494 127 2209
Fragments arranged by size:
2209 1494 410 217 127 75
MspA1I CmG'CkG
Cuts at: 1643 1957 2317 3330 3575 1643
Size: 314 360 1013 245 2600
Fragments arranged by size:
2600 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2713 2713
Size: 4532
NarI GG'CG_CC
Cuts at: 1963 2211 1963
Size: 248 4284
NgoMIV G'CCGG_C
Cuts at: 2711 2711
Size: 4532
NsiI A_TGCA'T
Cuts at: 592 921 592
Size: 329 4203
NspI r_CATG'y
Cuts at: 210 590 1032 1329 1585 2085 2992 210
Size: 380 442 297 256 500 907 1750
Fragments arranged by size:
1750 907 500 442 380 297 256
PciI A'CATG_T
Cuts at: 206 1028 2988 206
Size: 822 1960 1750
Fragments arranged by size:
1960 1750 822
PfoI T'CCnGG_A
Cuts at: 1462 1462
Size: 4532
PmeI GTTT'AAAC
Cuts at: 1531 1531
Size: 4532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 3671
Size: 32 4500
PpuMI rG'GwC_Cy
Cuts at: 280 280
Size: 4532
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 823 855 823
Size: 32 4500
PstI C_TGCA'G
Cuts at: 1579 2264 1579
Size: 685 3847
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4532
PvuII CAG'CTG
Cuts at: 1957 2317 1957
Size: 360 4172
RsrII CG'GwC_CG
Cuts at: 2727 2727
Size: 4532
SacI G_AGCT'C
Cuts at: 731 1546 731
Size: 815 3717
SalI G'TCGA_C
Cuts at: 1569 1569
Size: 4532
SanDI GG'GwC_CC
Cuts at: 280 280
Size: 4532
SapI GCTCTTCn'nnn_
Cuts at: 2555 2765 2555
Size: 210 4322
SbfI CC_TGCA'GG
Cuts at: 1579 1579
Size: 4532
ScaI AGT'ACT
Cuts at: 4049 4049
Size: 4532
SfcI C'TryA_G
Cuts at: 33 1575 2260 3253 3444 33
Size: 1542 685 993 191 1121
Fragments arranged by size:
1542 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1964 2212 1964
Size: 248 4284
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4532
SmaI CCC'GGG
Cuts at: 201 1554 201
Size: 1353 3179
SmlI C'TyrA_G
Cuts at: 765 1331 1687 2602 3094 3356 765
Size: 566 356 915 492 262 1941
Fragments arranged by size:
1941 915 566 492 356 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4532
SphI G_CATG'C
Cuts at: 590 1329 1585 2085 590
Size: 739 256 500 3037
Fragments arranged by size:
3037 739 500 256
SspI AAT'ATT
Cuts at: 379 1206 379
Size: 827 3705
StyI C'CwwG_G
Cuts at: 1148 1666 2020 1148
Size: 518 354 3660
Fragments arranged by size:
3660 518 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 447 882 994 2115 2248 2707 2916 447
Size: 435 112 1121 133 459 209 2063
Fragments arranged by size:
2063 1121 459 435 209 133 112
TatI w'GTAC_w
Cuts at: 1310 1514 4047 1310
Size: 204 2533 1795
Fragments arranged by size:
2533 1795 204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 497 529 4049 4081 497
Size: 32 3520 32 948
Fragments arranged by size:
3520 948 32 32
Tth111I GACn'n_nGTC
Cuts at: 2329 2329
Size: 4532
XbaI T'CTAG_A
Cuts at: 1563 1563
Size: 4532
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 324 2027 324
Size: 1703 2829
XhoI C'TCGA_G
Cuts at: 765 1331 765
Size: 566 3966
XmaI C'CCGG_G
Cuts at: 199 1552 199
Size: 1353 3179
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I BsiWI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI SacII SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XmnI ZraI